Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,579,943	G→A	G103D (GGC→GAC)	tktB →	transketolase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,579,943	0	G	A	100.0%	210.4 / NA	60	G103D (GGC→GAC)	tktB	transketolase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/31);  total (29/31)

TTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGA  >  NC_000913/2579652‑2580231                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                  ttGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGc                                                                                                                                                                                                                                                                                           <  1:371508/300‑1 (MQ=255)                       cAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAANCCTACCGACCCAACCTGGTATGATCGCGACCG‑NTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAaccaccacc                                                                                                                                                                                                                                                                       >  2:515384/1‑298 (MQ=255)                       cAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCAAGGCGTTGAAaccaccacc                                                                                                                                                                                                                                                                       >  2:157402/1‑298 (MQ=255)                       cAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCAGGAGATTGACTATACGCCAGGCGTTGAAaccaacaac                                                                                                                                                                                                                                                                       >  2:156931/1‑293 (MQ=255)                       cAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCAGCTGGAAGAACTGAAGAACTTACGTCAGTTGCA‑TTCGAAAACCCCAGGCCAACCGGAGATCGACTATACGCCAGGCGTTGAAaccaccacc                                                                                                                                                                                                                                                                       >  2:405048/1‑298 (MQ=255)                       cAGTATGGATGCGGTACAAAAAGCAAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAATGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCC‑TTCGAAAACCCACGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAaccaccacc                                                                                                                                                                                                                                                                       >  2:404976/1‑298 (MQ=255)                         gTATGGATGCGGTACACAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGCCCTGCCGCTGGAAGAACTGAAGAACTTCCGCCAGTTGCA‑TTCGAAAACCCAAGGCAACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACTGGcc                                                                                                                                                                                                                                                                   >  2:113835/1‑300 (MQ=255)                         gTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGcc                                                                                                                                                                                                                                                                   >  1:353321/1‑300 (MQ=255)                         gTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTACGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGcc                                                                                                                                                                                                                                                                   >  1:276387/1‑300 (MQ=255)                         gTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATACTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTAACGACCCAAACTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGACGTTGAAACCACCCCCGGcc                                                                                                                                                                                                                                                                   >  2:562492/1‑300 (MQ=255)                                   ggTACAAAAAGCCAACTCTGTTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGTAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGAc                                                                                                                                                                                                                                                          <  1:156931/299‑1 (MQ=255)                                   ggTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGAc                                                                                                                                                                                                                                                          <  1:515384/299‑1 (MQ=255)                                   ggTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGAc                                                                                                                                                                                                                                                          <  1:157402/299‑1 (MQ=255)                                    gTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACaa                                                                                                                                                                                                                                                        >  1:30676/1‑300 (MQ=255)                                            aGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGc                                                                                                                                                                                                                                                >  1:528252/1‑300 (MQ=255)                                             gCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCg                                                                                                                                                                                                                                               <  1:76493/300‑1 (MQ=255)                                                                    gcCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGTAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGc                                                                                                                                                                                                                       <  2:309384/300‑1 (MQ=255)                                                                    gcCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGc                                                                                                                                                                                                                       <  2:600200/300‑1 (MQ=255)                                                                    gcCGATGGGCAGGGCTGATATTGCCGAAGTGCTGTGGAATGATTGTCTTAAACATAACCCTACCAACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCATGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGc                                                                                                                                                                                                                       <  2:274673/300‑1 (MQ=255)                                                                       gATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCTGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCag                                                                                                                                                                                                                     <  2:71135/299‑1 (MQ=255)                                                                               tGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCATGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTacac                                                                                                                                                                                                            <  2:352809/300‑1 (MQ=255)                                                                                        ttGCCGAAGTGCTTGGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATAATCGCGACCG‑TTTTATGCTTTCCAACGGGCACGCGTTGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCACTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCATGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTacac                                                                                                                                                                                                            <  2:352549/291‑1 (MQ=255)                                                                                                gTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTAAAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTACGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAAc                                                                                                                                                                                           >  1:251074/1‑300 (MQ=255)                                                                                                        gAACGATTTTCTTAAANATAACCCTACCGACCCAANCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTacacctgtctcntatacacatctcgcag                                                                                                                                                                                    <  1:583674/299‑25 (MQ=255)                                                                                                                       aCATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGAtcgtc                                                                                                                                                                      >  2:390135/1‑298 (MQ=255)                                                                                                                       aCATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGa                                                                                                                                                                    >  1:519441/1‑300 (MQ=255)                                                                                                                       aCATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGACCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGAAGTTTAACCAGCCAGACCATGGGATCGTCGa                                                                                                                                                                    >  2:153483/1‑300 (MQ=255)                                                                                                                            aCCCTACCGACCCAACCTGGTATGATCGCGACCGGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGGAGAACTGAAGCACTTCCGTCAGTTGCACGTCGAAAACCCCAGGCCCCCCGGAGATTGACTATCCGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAAGCGCCGTCGGGCTGGCGATAGCAGAGCGt                                                                                                                                                                                                                >  2:571902/1‑254 (MQ=255)                                                                                                                            aCCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACt                                                                                                                                                               >  1:196150/1‑300 (MQ=255)                                                                                                                                    gACCCAACCTGTTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTTAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATTAGATCGTCGATCACTTCACCTa                                                                                                                                                        <  2:669812/299‑1 (MQ=255)                                                                                                                                    gACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTa                                                                                                                                                        <  1:602421/299‑1 (MQ=255)                                                                                                                                    gACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACANCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTa                                                                                                                                                        <  1:137496/299‑1 (MQ=255)                                                                                                                                     aCCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCACAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCAACTAt                                                                                                                                                       >  2:238075/1‑299 (MQ=255)                                                                                                                                         aaCCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACTCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGANAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTAtgtg                                                                                                                                                    <  2:210945/298‑1 (MQ=255)                                                                                                                                                                   aTTCTTTCCCACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCAGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGaa                                                                                                                         >  1:446298/1‑300 (MQ=255)                                                                                                                                                                   aTTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGaa                                                                                                                         >  1:445837/1‑300 (MQ=255)                                                                                                                                                                         tCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCTGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTAtt                                                                                                                   <  2:400719/300‑1 (MQ=255)                                                                                                                                                                                   aCGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAg                                                                                                         <  1:373742/300‑1 (MQ=255)                                                                                                                                                                                               tgctCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTgg                                                                                             <  1:110072/300‑1 (MQ=255)                                                                                                                                                                                                gctCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGCCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTggc                                                                                            >  1:434482/1‑300 (MQ=255)                                                                                                                                                                                                   ctACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGc                                                                                                                                                                                                                                 <  2:419087/164‑1 (MQ=255)                                                                                                                                                                                                   ctACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGc                                                                                                                                                                                                                                 >  1:419087/1‑164 (MQ=255)                                                                                                                                                                                                              tACATCTGACCGGTTACGATCTGCCGCTGGAAGAACTGAAGAACTTCCGTTATTTGCA‑TTCGAAAACCCCATGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCTGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGTCGGCGCAGTTTAACCAGCCAGACCATGAGGTCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGAc                                                                              <  2:280076/300‑1 (MQ=255)                                                                                                                                                                                                              tACATCTGACCGGTTACGACCTGCCGCTGTAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGTAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTTGCAGGCACGCTGGGAc                                                                              <  2:362407/300‑1 (MQ=255)                                                                                                                                                                                                              tACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTTAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGTCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTTGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCAATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGAc                                                                              <  2:331223/300‑1 (MQ=255)                                                                                                                                                                                                              tACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGAc                                                                              <  2:666046/300‑1 (MQ=255)                                                                                                                                                                                                              tACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGAc                                                                              <  1:519831/300‑1 (MQ=255)                                                                                                                                                                                                               aCATCTGACCGGTTACGACCTGCCGCTGGAAGAACTTAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCNGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCTGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCGATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTTGGAc                                                                              <  1:493838/299‑1 (MQ=255)                                                                                                                                                                                                                         ggTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCt                                                                    >  1:634619/1‑299 (MQ=255)                                                                                                                                                                                                                          gTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGa                                                                  >  1:323620/1‑300 (MQ=255)                                                                                                                                                                                                                               gACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGTAGATTGACTATACGCCAGGCGTTGAAACCACCACCTGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGNGCAGTTTAACCAGCCAGACCATGAGATCGTCGNTCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTgg                                                              <  2:519441/299‑1 (MQ=255)                                                                                                                                                                                                                                       gCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCATGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGACCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACg                                                      <  2:319362/299‑1 (MQ=255)                                                                                                                                                                                                                                          ggAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCAACTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATca                                                  >  2:161961/1‑300 (MQ=255)                                                                                                                                                                                                                                          ggAAGAACTGAAGAACTTACGTCAGTTGCA‑TTCGAAAACCCCAGGCCAACCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCACGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAAACAGCCAGACCATGAGATCGTCGATCAATTCACCTATGTGTTCATGGGCGACAGCTGCCTGATGGAAGGTATTTCCCAAGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACAATca                                                  >  2:161887/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 ccccAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCANGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGt            >  1:134251/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          aCCCTGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACcga     <  2:446298/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                          aCCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACcgac    >  1:336906/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                          aCCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACcga     <  2:445837/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                          aCCCGGAGAGTGACTATACACCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACcgac    <  2:30676/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                           cacGGAGATTGACTATACGCCAGGCGTTGAAACCACCAACGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTCCcgccga  >  2:350394/3‑300 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                  TTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCG‑CTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCA‑TTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAA‑CGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGA  >  NC_000913/2579652‑2580231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑