Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,125,037	G→A	S237F (TCC→TTC)	wcaI ←	colanic acid biosynthesis fucosyltransferase WcaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,125,037	0	G	A	100.0%	209.5 / NA	62	S237F (TCC→TTC)	wcaI	colanic acid biosynthesis fucosyltransferase WcaI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/31);  total (31/31)

CTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGGAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGTTCGAACGCCGTTGCCAGCTGTGCCACTTTGCCGCCTTTGCCTTTTCCGGCAAGGCCCAGCCCCAGCATGGCGTCCACTTC  >  NC_000913/2124756‑2125326                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                   cTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGAtttttt                                                                                                                                                                                                                                                                                  >  2:25262/1‑300 (MQ=255) cTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCATGCTTTTCACCAATATTGCCGAAGTAAAGAATGAtttttt                                                                                                                                                                                                                                                                                  >  2:406938/1‑300 (MQ=255)         atatTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCTCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGATTCAATAACGTTTTCCAGCCCATGCTTTTCAACAATATTGCCGACGTAAGGAATGATTTTTTTGTTATct                                                                                                                                                                                                                                                                          >  2:560489/1‑299 (MQ=255)                      tctaCGGCAATACTGCATCTGTCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCt                                                                                                                                                                                                                                                             <  2:476601/297‑1 (MQ=255)                           ggCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGTAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCGTGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAATGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCTGCAGGTCAAGCTGGTTa                                                                                                                                                                                                                                                        <  2:133330/300‑1 (MQ=255)                            gCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTAc                                                                                                                                                                                                                                                       >  1:191432/1‑300 (MQ=255)                              aaTACGGCATCTGCCGCNCCGCGTTTTTGCACCACCNGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCANGTCAAGCTGGTTACGa                                                                                                                                                                                                                                                     <  1:578411/300‑1 (MQ=255)                                         tGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAAc                                                                                                                                                                                                                                          <  1:443957/300‑1 (MQ=255)                                         tGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGAATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCaa                                                                                                                                                                                                                                           <  1:390574/299‑1 (MQ=255)                                                  gcgTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCa                                                                                                                                                                                                                                   <  1:14979/298‑1 (MQ=255)                                                          gCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCTGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGGAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACAt                                                                                                                                                                                                                         <  2:581564/300‑1 (MQ=255)                                                          gCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGTTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACAt                                                                                                                                                                                                                         <  2:182180/300‑1 (MQ=255)                                                          gCACCACCAGATGGCAATCACCCATCTTCAGCAGTGCGGTTAAAGCGTCATACGATTGCAGCGGTAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACAt                                                                                                                                                                                                                         <  2:182291/300‑1 (MQ=255)                                                            accaccAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGTCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACAt                                                                                                                                                                                                                         <  2:183414/298‑1 (MQ=255)                                                                ccaGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCATGCTTTTCACCAATATTGCAGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCCACATCGGCATCTGCAACATGCTGaa                                                                                                                                                                                                                   >  2:239250/1‑300 (MQ=255)                                                                              cccATCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTcc                                                                                                                                                                                                     >  1:398214/1‑300 (MQ=255)                                                                                 aTCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGc                                                                                                                                                                                                                                    >  2:111143/1‑266 (MQ=255)                                                                                 aTCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGc                                                                                                                                                                                                                                    <  1:111143/266‑1 (MQ=255)                                                                                   cTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCa                                                                                                                                                                                                >  1:534014/1‑300 (MQ=255)                                                                                               gggTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTNAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGAACAGTTGGGgaagaa                                                                                                                                                                                    >  1:150841/1‑300 (MQ=255)                                                                                                                     gcgagatgtgtataagagacaggcAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCAc                                                                                                                                                              >  1:680415/23‑299 (MQ=255)                                                                                                                                         gcgcAGTCCACGCTGCNTGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGACGAAGATGNGCGTTTCCCCCCACCACGNCTTTTTCAGTGGCttt                                                                                                                                            >  1:576565/1‑299 (MQ=255)                                                                                                                                              gTCCACGCTGC‑TGCGCCATTTTTTCCCGCCGCGCTTTGCCGCCGCCCTGCCCGACACTGGCAAAAATCAGCGGTTCATCGCGCAGACGCTCGGCAGCTTCAATAACGTTTTCCAGCCACTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTCTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGCAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGGTGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGTTTTATtcatc                                                                                                                                     >  2:598637/1‑299 (MQ=255)                                                                                                                                              gTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATtcatca                                                                                                                                    >  1:39799/1‑300 (MQ=255)                                                                                                                                              gTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATtcatca                                                                                                                                    >  2:661627/1‑300 (MQ=255)                                                                                                                                              gTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGAACAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATtcatca                                                                                                                                    >  1:95613/1‑300 (MQ=255)                                                                                                                                              gTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGAACAGTTGGGGAAGAAGATGA‑CGTTTTCCGACGCCACGCCTTTTTCGATGGCTTTATtcatca                                                                                                                                    >  2:454914/1‑300 (MQ=255)                                                                                                                                              gTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTCCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTAAAAACGGGCAATTTCCGAACAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTCCGATGGCTTTATtcatca                                                                                                                                    >  1:225654/1‑300 (MQ=255)                                                                                                                                               tCCACGCTGC‑TGCGCCATTTTTTCCAGCTACGCTTGGCCGTCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAAGAACGTTTTCCAGCCCCTGCTTGTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGTTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAGGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTcatcat                                                                                                                                   <  2:9296/300‑1 (MQ=255)                                                                                                                                                  aCGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCAACAATATTGCAGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGAACAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGa                                                                                                                                >  2:36631/1‑300 (MQ=255)                                                                                                                                                  aCGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTGCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTGTATTcatcat                                                                                                                                   <  2:9385/297‑1 (MQ=255)                                                                                                                                                     ctgc‑tgCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAAc                                                                                                                              >  2:483956/1‑299 (MQ=255)                                                                                                                                                                   ttttCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGgag                                                                                                                <  1:554292/300‑1 (MQ=255)                                                                                                                                                                   ttgtCCAGCCGTGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGgag                                                                                                                <  1:230856/297‑1 (MQ=255)                                                                                                                                                                      tCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACg                                                                                                             <  1:101193/300‑1 (MQ=255)                                                                                                                                                                        cAGCCGCCCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGtt                                                                                                           >  1:390731/1‑300 (MQ=255)                                                                                                                                                                             gcgcTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATc                                                                                                        <  2:380581/298‑1 (MQ=255)                                                                                                                                                                             gcgcTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGa                                                                                                      <  1:58460/300‑1 (MQ=255)                                                                                                                                                                                       gccgccCTGCCCGACAACTGCAAAAATCAGCGGTTCATCGCGCAGACGATCTGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAg                                                                                            <  2:293216/300‑1 (MQ=255)                                                                                                                                                                                          gccCTGCCCGACCAGGGCAAAAATCAGCGGTTCATCGCGTAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCCGGTTACGAAGGGCATCAACATCGGGATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGTGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCAGCATCGAACGCGAAAGCGTGGAGACGTTATCGACGTTATGCat                                                                                            <  2:470859/297‑2 (MQ=255)                                                                                                                                                                                             cTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGNTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAAAGGGCAATTTCCGAACAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCAAGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCt                                                                                      >  2:497428/1‑300 (MQ=255)                                                                                                                                                                                               gccggACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTAc                                                                                    <  1:160401/296‑1 (MQ=255)                                                                                                                                                                                               gCCCGACAATTGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCTGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGTAGACGTTATCGACGTTATGCAGTCCGCTAc                                                                                    <  1:603694/300‑1 (MQ=255)                                                                                                                                                                                               gCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGTAGACGTTATCGACGTTATGCAGTCCGCTAc                                                                                    <  1:541487/300‑1 (MQ=255)                                                                                                                                                                                               gCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGTAGACATTATCGACGTTATGCAGTCCGCTAc                                                                                    <  1:560489/300‑1 (MQ=255)                                                                                                                                                                                               gCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTAc                                                                                    >  1:545518/1‑300 (MQ=255)                                                                                                                                                                                                        tGGCAAAAATCAGCGGTTCGTCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCTAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGACTTTTTTGTTATCCGGCAGGTCAAGCTGTTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGTAGACGTTATCGa                                                                                                      <  2:200077/273‑1 (MQ=255)                                                                                                                                                                                                               aaTCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGTTCGAACGCCGTTGc                                                                    >  1:221663/1‑300 (MQ=255)                                                                                                                                                                                                                   aGCGGTTCATCGCGCAGACGATCGGCAGCTTCAGTAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGTTCGAACGCCGTTGCCAGc                                                                <  1:400549/300‑1 (MQ=255)                                                                                                                                                                                                                   aGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGTGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGTAGACGTTATCGACGTTATGCAGTCCGCTACGTTCGAACGCCGTTGCCAGc                                                                <  2:191432/300‑1 (MQ=255)                                                                                                                                                                                                                                   gACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAAAGCGAAGTCGTGGAGACGTTATCGACGTTATGCAGTCCGCTAAGTTCGAACGCCGTTGCCAGCTGTGCCACTTTgccgc                                                >  2:63722/1‑300 (MQ=255)                                                                                                                                                                                                                                    aCGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCAGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGAGCAATTTCCGAACAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGACCGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTAAGTTCGAACGCCATTGCCAGCTGTGCCACTTTgcctcc                                               >  2:30596/1‑297 (MQ=255)                                                                                                                                                                                                                                          ggCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGTTCGAACGCCGTTGCCAGCTGTGCCACTTTGCCgcctttgcc                                         <  1:549126/300‑1 (MQ=255)                                                                                                                                                                                                                                           gCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGTTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGTAGACGTTATCGACGTTATGCAGTCCGCTACGTTCGAACGCCGTTGCCAGCTGTGCCACTTTGCCgcctttgcct                                        <  2:273666/300‑1 (MQ=255)                                                                                                                                                                                                                                             aGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCGAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGATGTTATCGACGTTATGCAGTCCGCTACGTTCGAATGCAGTTGCCCGCTGTGCCACTTTGCCgcctttgccttt                                      >  2:83275/1‑300 (MQ=255)                                                                                                                                                                                                                                               cTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGTTCGAACGCCGTTGCCAGCTGTGCCACTTTGCCGCCTTTGCCTTTTc                                    >  2:688546/1‑300 (MQ=255)                                                                                                                                                                                                                                                 tCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGTTCGAACGCCGTTGCCAGCTGTGCCACTTTGCCGCCTTTGCCTTTTcc                                   >  1:556154/1‑299 (MQ=255)                                                                                                                                                                                                                                                            ttCCAGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGTTCGAACGCCGTTGCCAGCTGTGCCACTTTGCAGCCTTTGCCTTTTCCGGCAAGGccca                        >  2:571228/1‑299 (MQ=255)                                                                                                                                                                                                                                                                aGCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGNCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGTTCGAACGCCGTTGCCAGCTGTGCCACTTTGCCGCCTTTGCCTTTTCCGGCAAGGcccagcccc                   <  1:133217/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 gCCCCTGCTTTTCACCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGTTCGAACGCAGTTGCCAGCTGTGCCACTTTGCCGCCTTTGCCTTTTCCGGCAAGGcccagcccca                  >  1:233670/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              aCCAATATTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGTTCGAACGCCGTTGCCAGCTGTGCCACTTTGCCGCCTTTGCCTTTTCCGGCAAGGCCCAGCCCCAGCATGGCGTCCAc     <  1:392112/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  atatTGCCGAAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGTTCNAACGCCGTTGCCAGCTGTGCCACTTTGCCGCCTTTGCCTTTTCCGGCAAGGCCCAGCCCCAGCATGGCGTCCACTTc  >  1:503013/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                   CTGCCAGAATATTGGTCAGTTTCGACGGCAATACGGCATCTGCCGCGCCGCGTTTTTGCACCACCAGATGGCAATCGCCCATCTTCAGCAGTGCGGGTAAAGCGTCATACGATTGCAGCGGGAAAAATTGCATGTTGCGCAGTCCACGCTGC‑TGCGCCATTTTTTCCAGCCGCGCTTTGCCGCCGCCCTGCCCGACAATGGCAAAAATCAGCGGTTCATCGCGCAGACGATCGGCAGCTTCAATAACGTTTTCCAGCCCCTGCTTTTCACCAATATTGCCGGAGTAAAGAATGATTTTTTTGTTATCCGGCAGGTCAAGCTGGTTACGAAGGGCATCAACATCGGCATCTGCAACATGCTGAAAACGGGCAATTTCCGACCAGTTGGGGAAGAAGATGA‑CGTTTTCCGCCGCCACGCCTTTTTCGATGGCTTTATTCATCATCGAACGCGAAATCGTGGAGACGTTATCGACGTTATGCAGTCCGCTACGTTCGAACGCCGTTGCCAGCTGTGCCACTTTGCCGCCTTTGCCTTTTCCGGCAAGGCCCAGCCCCAGCATGGCGTCCACTTC  >  NC_000913/2124756‑2125326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑