Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,213	A→C	S190A (TCG→GCG)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,213	0	A	C	100.0%	218.5 / NA	60	S190A (TCG→GCG)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/38);  total (22/38)

GAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGC  >  NC_000913/64926‑65497                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                             gAACGCGGATTTCAGCGCCTCGATACCGTCGAGAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGcc                                                                                                                                                                                                                                                                                  <  2:442546/300‑1 (MQ=255)   aCGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGTGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCAt                                                                                                                                                                                                                                                                                <  2:119085/300‑1 (MQ=255)            tCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGcc                                                                                                                                                                                                                                                                       <  1:427741/300‑1 (MQ=255)            tCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTAACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGcc                                                                                                                                                                                                                                                                       <  1:443123/300‑1 (MQ=255)                  ccTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCa                                                                                                                                                                                                                                                                 >  1:190799/1‑300 (MQ=255)                         aCCGTCGATAATTAGCCNACCTTTAGCCTGGGCAAANAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTNGCCCCAGCATATAAAc                                                                                                                                                                                                                                                          <  1:571375/300‑1 (MQ=255)                         aCCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAAc                                                                                                                                                                                                                                                          <  1:420413/300‑1 (MQ=255)                                            ccTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATNGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCACGCAGCCt                                                                                                                                                                                                                                       >  2:219204/1‑300 (MQ=255)                                            ccTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCt                                                                                                                                                                                                                                       >  2:692274/1‑300 (MQ=255)                                            ccTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCAAATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCt                                                                                                                                                                                                                                       >  2:119375/1‑300 (MQ=255)                                                     tGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGNAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCATGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGc                                                                                                                                                                                                                                <  1:494617/298‑1 (MQ=255)                                                     tGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGNAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGc                                                                                                                                                                                                                                <  1:494613/298‑1 (MQ=255)                                                              aaGACTCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCACAGCCGCAGCGGAATACGTTAATGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCANGCGTTGAGTTTTTCCAGTAGCTGCGAGCGGCTNGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCAGTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTAc                                                                                                                                                                                                                     <  2:511095/300‑1 (MQ=255)                                                              aaGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAggac                                                                                                                                                                                                                     <  1:341672/300‑4 (MQ=255)                                                              aaGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTAc                                                                                                                                                                                                                     <  1:322961/300‑1 (MQ=255)                                                              aaGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTAc                                                                                                                                                                                                                     <  2:390787/300‑1 (MQ=255)                                                                gACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCANGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTNGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTAc                                                                                                                                                                                                                     <  2:511107/298‑1 (MQ=255)                                                                   gCCGTTTTTAAGGCCGTACTCGCGCCACTCTAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTTGCGACGTATTCCAGTTCAAAATCGAGCGCGTAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAtctc                                                                                                                                                                                                                <  2:43344/300‑1 (MQ=255)                                                                   gCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCNCATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCTGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCGGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTc                                                                                                                                                                                                                <  2:222444/300‑1 (MQ=255)                                                                   gCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGTAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTc                                                                                                                                                                                                                <  1:548545/300‑1 (MQ=255)                                                                        ttttAAAGCCGTGCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGt                                                                                                                                                                                                           <  1:685951/300‑1 (MQ=255)                                                                                    gCTCGCGCCACTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTAcctgtctcttatacacatctcgc                                                                                                                                                                                               <  1:254798/300‑23 (MQ=255)                                                                                             aCTCCAGCTCACTGTTATCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTACAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCGTTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCCGCCTTCCAGGCCGATGCAGTACAGCTAACCGTGGCGGGTGGTTTCAATATca                                                                                                                                                                                       >  2:192642/1‑298 (MQ=255)                                                                                                     tcactgttatCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAAACGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAAcc                                                                                                                                                                              <  1:324322/294‑1 (MQ=255)                                                                                                        ctgttatCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCAc                                                                                                                                                                           >  1:681756/4‑300 (MQ=255)                                                                                                        ctgttatCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCACTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCAc                                                                                                                                                                           >  1:291480/4‑300 (MQ=255)                                                                                                           ttatCGCGCCCCAGCCGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTg                                                                                                                                                                        <  2:52582/300‑1 (MQ=255)                                                                                                                        gccGCAGCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCTTATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGAt                                                                                                                                                           <  2:602405/298‑1 (MQ=255)                                                                                                                           gCAGCGGAATACGGTAACACTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGTAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGAt                                                                                                                                                           <  2:68609/297‑1 (MQ=255)                                                                                                                              gCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTc                                                                                                                                                       >  1:196649/1‑298 (MQ=255)                                                                                                                              gCGGAATACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCAAATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTc                                                                                                                                                       >  1:196804/1‑298 (MQ=255)                                                                                                                                 gaataCGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCAAATCAGGATCGTAGTTGGCAAACTAGGCGTTGAGTTTTTACAGTAGCTGCGGGCGGCTGGCGACGTATTCAAGTTCAAAATCGAGCGCGGAGGCGTCGCCCTTCTCTGGCCCCAGCATATACACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCACTATCAATAGAAACCCACTTGAGCGGCGGACGATGGTCGGGAt                                                                                                                                                  >  2:351820/5‑300 (MQ=255)                                                                                                                                   ataCGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGTCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGc                                                                                                                                                >  1:649462/3‑300 (MQ=255)                                                                                                                                                       tttttGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAAc                                                                                                                            >  1:203223/1‑300 (MQ=255)                                                                                                                                                               gcaTTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGTGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATa                                                                                                                        >  2:279735/1‑296 (MQ=255)                                                                                                                                                                       aGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGc                                                                                                            <  1:260378/300‑1 (MQ=255)                                                                                                                                                                            gAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGNGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATc                                                                                                       <  1:487308/300‑1 (MQ=255)                                                                                                                                                                            gAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATc                                                                                                       <  1:219408/300‑1 (MQ=255)                                                                                                                                                                            gAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGCGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATc                                                                                                       <  2:258609/300‑1 (MQ=255)                                                                                                                                                                            gAACTGCACCACGTTCCAACCGATGATCAAATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGACATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATc                                                                                                       >  2:328798/1‑300 (MQ=255)                                                                                                                                                                            gAACTACACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGNGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTGCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATc                                                                                                       <  1:127256/300‑1 (MQ=255)                                                                                                                                                                             accTGCACCACGTTCCAACCGATGAGCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGTGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATc                                                                                                       <  2:379166/297‑1 (MQ=255)                                                                                                                                                                             aaCTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTAGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCa                                                                                                      >  1:559366/1‑300 (MQ=255)                                                                                                                                                                                 gCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCATGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGNCTTCCAGGCCGATGCAGTACAGCTCACCGTGGNGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCt                                                                                                  <  2:516168/300‑1 (MQ=255)                                                                                                                                                                                 gCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGTCGGCTGTCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATGACCGATGCGCTGCCCGCAGNCTTCCATGCCGATGCAGTACAGCTCACCGTGGNGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATGGTGCATATCACCCt                                                                                                  <  2:516160/300‑1 (MQ=255)                                                                                                                                                                                 gCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCAGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCt                                                                                                  >  1:652261/1‑300 (MQ=255)                                                                                                                                                                                       cGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGAccc                                                                                            >  1:47781/1‑300 (MQ=255)                                                                                                                                                                                             aaCCGATGATCACATCAGGATCTTAGGTGGCAAACCAGTCTTTGAGTTTTTCCAGTAGCTGCGTTCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCTACCCacac                                                                                        <  2:428002/298‑1 (MQ=255)                                                                                                                                                                                             aaCCGATGATCACATCAGGATCGTAGTTGGCAAACCATGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGTATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACAcc                                                                                       <  2:679026/299‑1 (MQ=255)                                                                                                                                                                                             aaCCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACNTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTAGACCCacac                                                                                        <  1:133131/298‑1 (MQ=255)                                                                                                                                                                                               ccGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCggt                                                                                    >  2:637719/1‑300 (MQ=255)                                                                                                                                                                                                           tCAGGATCGTAGTTGGCAAACCATGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAAc                                                                        <  1:320050/300‑1 (MQ=255)                                                                                                                                                                                                           tCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGTCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTGCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAAc                                                                        <  1:320923/300‑1 (MQ=255)                                                                                                                                                                                                           tCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAAc                                                                        <  1:323078/300‑1 (MQ=255)                                                                                                                                                                                                              ggATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGc                                                                     >  2:397239/1‑300 (MQ=255)                                                                                                                                                                                                                                   gCGTTGAGTTTTTCCAGTAGCTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCAATCAGATAGCGTTCt                                                   >  1:272500/1‑297 (MQ=255)                                                                                                                                                                                                                                       tgattttttccattagcTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTTGCCCCAGCATATAGACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTANAGCTCACCTTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCGGATAGCGTTCTGGCg                                               <  2:210503/281‑1 (MQ=255)                                                                                                                                                                                                                                                 cagtagcTGCGGGCGGCTGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGc                                  >  1:523419/7‑300 (MQ=255)                                                                                                                                                                                                                                                                  tGGCGACGTATTCCAGTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATACCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGTTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAAc                   <  2:559366/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                 gTTCAAAATCGAGCGCGGAGGCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACAGCTCCATCAGATAGCGTTCTGGCGTGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCAAGCAGgc  >  2:365829/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                             GAACGCGGATTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCTCGGCATGTTTTTGCAGCATTCGCAGATCGAACTGCACCACGTTCCAACCGATGATCACATCAGGATCGTAGTTGGCAAACCAGGCGTTGAGTTTTTCCAGCAACTGCGGGCGGCTGGCGACGTATTCCAGTTCGAAATCAAGCGAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGC  >  NC_000913/64926‑65497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑