| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 76,283 | C→T | L339L (CTG→CTA) | sgrR ← | DNA‑binding transcriptional dual regulator SgrR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 76,283 | 0 | C | T | 98.8% | 295.8 / NA | 86 | L339L (CTG→CTA) | sgrR | DNA‑binding transcriptional dual regulator SgrR |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (47/38); minor base G (0/1); total (47/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTCTTTGATTTTCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTTAGGGTGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACG‑GACCGGT‑GCCGATGGGATGGCTGGC‑AAAGTTA‑CTGAGG‑GTTTCCCATTCGCGCGGC‑AGGATCATCGCCGGAACTTGCCCCA > NC_000913/75988‑76572 | tctcTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGCTCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTa > 1:516099/1‑300 (MQ=255)tctcTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGCCTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTa > 1:516105/1‑300 (MQ=255) tttGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTTTGATCCTGATAAAAGGTCAGGATGAGGCTTTCAAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGTAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAAt < 1:155474/299‑1 (MQ=255) tttGATTTCCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCAAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAAt < 1:154296/299‑1 (MQ=255) tgatttcaaGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAAtagt < 1:426361/292‑1 (MQ=255) aGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCt < 1:206177/300‑1 (MQ=255) aCCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTtcctcc > 1:100626/1‑300 (MQ=255) aCCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACCGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTtcctcc < 2:583103/300‑1 (MQ=255) cTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCa > 2:295716/1‑299 (MQ=255) gctgcATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTTCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCttttt > 1:657154/1‑300 (MQ=255) gcATGATCCCGGCAATCACCCGATGTTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTAAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTACTCAAGGCGGTTTTCAATCTCTTTTTCCt > 2:368819/1‑300 (MQ=255) gcATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCt > 1:321135/1‑300 (MQ=255) aTCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGTTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCAccc < 2:631673/300‑1 (MQ=255) aTCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCTAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCTGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCAccc < 2:360149/300‑1 (MQ=255) aaTCACCCGATACTCACTGTGATCCTGAGAGAAGGTCAGGATGAGGCTTTCCAGTCCAGCCGGTTTTTCGCTCGTTATGGTGCGGGCATGGTGCCAACGGTGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCATCGAAAGAGACCAGATTAGTTTGAGAAAGCACATAGCTTACCCAGTCCCTGACTGGCTGA‑TTCGCCCCGCGAGGGGTGCTGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCGTTTTCCTCGCCCGGTGGAcc < 2:615973/300‑1 (MQ=255) cccGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAAt > 2:291284/1‑271 (MQ=255) cccGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAAt < 1:625839/271‑1 (MQ=255) cccGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAAt > 2:625839/1‑271 (MQ=255) cccGATGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAAt < 1:291284/271‑1 (MQ=255) tGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCNCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATc > 2:498694/1‑300 (MQ=255) tGCTCACTGTGATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCNCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTAACCCTGTGGACCTTTTAGCAt > 2:498684/1‑299 (MQ=255) tgtgATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTc < 1:592244/300‑1 (MQ=255) gATCCTGATAAAGGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGATCTTTATGGTGCGGGCATGGTGCAAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCATCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAAACTTCCTCAAGGCGGCTTTCAATCTCTTTTTCCTCACCCTTTGGGCCTTTTAGCATCAGACCTcc > 2:400921/1‑298 (MQ=255) gATCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTcca > 2:400929/1‑298 (MQ=255) aTCCTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTccagc < 2:378450/300‑4 (MQ=255) ccTGATAAAAGGTCAGGATGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCagct > 2:187722/1‑299 (MQ=255) tttCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCAAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTAACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGc > 2:136457/1‑296 (MQ=255) ttCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAAc > 1:542112/1‑300 (MQ=255) aGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGTAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAAccc < 2:677546/298‑1 (MQ=255) aGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAAccc < 2:677508/298‑1 (MQ=255) ggCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGa < 2:453120/300‑1 (MQ=255) gaccAGCCGGTTTTTCGCTCTTTATTGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGa < 2:183384/298‑1 (MQ=255) aGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACg < 1:540701/298‑1 (MQ=255) aGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGtt > 1:527224/1‑300 (MQ=255) aGCCGGTTTTTCACTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCATATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACg < 2:242519/298‑1 (MQ=255) tttttCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACtt < 2:328845/299‑1 (MQ=255) ctctTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGAtt > 1:458974/1‑300 (MQ=255) ctTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGANAGCACATAGCTTACCCAGTCCCTGACTTGCTGN‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTAGTCGATTaa > 2:519332/1‑300 (MQ=255) ctTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTaa > 1:387802/1‑300 (MQ=255) ttctGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAAt > 1:382128/4‑299 (MQ=255) tgtGGTGCGGGCATGGTGCCAACGTGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTTGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACTTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTGTAGCATCAGACCTCCAGCTGGCTCGTCGGCGATTTCCGGTAGAGCCCAGACGTTGACTTTGTCGGTTAGTGc < 2:260316/298‑1 (MQ=255) gTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCTTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGccc > 1:421586/1‑298 (MQ=255) gggCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAAc > 2:417584/1‑300 (MQ=255) tGCCAACGTGGTAGCAGGCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCTAAATAGACCAGATTAGTTGTAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCATGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGaagaag < 2:103711/300‑1 (MQ=255) tGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGaagaag < 2:630479/300‑1 (MQ=255) aaCGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCACGGTAACCGAAGAAGTCAt > 2:319041/1‑300 (MQ=255) gggggAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAANCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGa < 1:498694/300‑1 (MQ=255) gggggAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAANCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGGAACTGAAGAAGTCATCGa < 1:498684/300‑1 (MQ=255) gTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAAtagtag < 2:611213/143‑1 (MQ=255) gTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAAtagtag > 1:611292/1‑143 (MQ=255) gTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAAtagtag < 2:611292/143‑1 (MQ=255) gTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAAtagtag > 1:611213/1‑143 (MQ=255) gAACCACAGTTGCTGGTACTGTTCCTCAGCTAAATAGACCAGATTAGTTGTAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGAtt < 2:391827/300‑1 (MQ=255) caGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACGCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTAGATTAATGCCCGGTAACCGAAGCAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCt > 2:566548/1‑300 (MQ=255) gTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTg > 1:74451/1‑296 (MQ=255) tGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCCGTTGATTGGTGCTGtt > 1:54869/1‑299 (MQ=255) tGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGtt > 1:415532/1‑299 (MQ=255) tGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCATCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACTGAAGAAGTCATCGAATGCCTGAATTTTCAGTCGATTGGTGCTGtt > 2:235176/1‑299 (MQ=255) tGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCAGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGNACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGtt > 2:220370/1‑299 (MQ=255) gTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCggat < 1:66441/300‑4 (MQ=255) agctaaaTAGACCAGATTAGTTGGAGAAAGCATATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGTACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGATGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACg < 2:370781/295‑1 (MQ=255) aGCGAAATAGACCAGATTAGTTGTAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCATGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACg < 2:267225/299‑1 (MQ=255) aGCGAAATAGACCAGATTAGTTGGATAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCTCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGATGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTACTGTTGCGGATCACCGCATACg < 2:370589/299‑1 (MQ=255) aaaTAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GAcc > 2:414005/1‑299 (MQ=255) aGATTAGTTGTAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GACCGGT‑GCCgatg < 2:41665/300‑1 (MQ=255) aGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GACCGGT‑GCCgatg < 2:41699/300‑1 (MQ=255) aGCTTCCCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GACCGGT‑GCCGATGGGATGGCTGGC‑AAAGTTA‑CTGa > 2:113755/1‑300 (MQ=255) aGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GACCGGT‑GCCGATGGGATGGCTGGC‑AAAGTTA‑CTGa > 1:269627/1‑300 (MQ=255) aGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCACGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GACCGGT‑GTCGATGGGATGGCTGGC‑AAAGtt > 2:315743/1‑295 (MQ=255) aGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTATTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCCGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GACCGGT‑GCCGATGGGATGGCTGGC‑AAAGTTA‑CTGa > 2:114675/1‑300 (MQ=255) aGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAAACTTCATACAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGATGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTAATCGAATGCCTGAATTTTCAGTTTATTGGTGCTGTTGCGGATCACCGCATACG‑GACCGGT‑GCAGATGGGGTGGGTGGC‑AAAGTTA‑Ct > 2:34393/1‑298 (MQ=255) aGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAACAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCACGGTAACCGAAGAAGTCATCGAATGTCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCACACG‑GAACGGT‑GCCGATGGGATGGCTGGA‑AAAGTTA‑Ct > 2:40918/1‑298 (MQ=255) gCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GAACGGT‑GCCGATGGGATGGCTGGC‑AAAGTTA‑CTGa > 2:333214/1‑299 (MQ=255) gCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAAGAGTAAAGAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GACCGGT‑GCCGATGGGATGGCTGGC‑AAAGTTA‑CTGAg < 2:555767/300‑1 (MQ=255) aGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GACCGGT‑GCCGATGGGATGGCTGGC‑AAAGTTA‑CTGAGG‑GTTTccc < 1:448415/300‑1 (MQ=255) aGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GACCGGT‑GCCGATGGGATGGCTGGC‑AAAGTTA‑CTGAGG‑GTTTccc < 2:594853/300‑1 (MQ=255) aGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGNTAACTTCGTCGATTAATGCCCGGTAACCGAAGNAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGCTGCGGATCACCGCATACG‑GACCGGT‑GCCGATGGGATGGCTGGC‑AAAGTTA‑CTGAGG‑GTTTccc < 2:516105/300‑1 (MQ=255) aGTCCCTGACGTGCTTA‑TTCGCCCCGCGATGGGTGCGACTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGTCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACTTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGNTAACTTCGTCGATTAATGCCCGGTAACCGAAGNAGTCATCGAATGCCTGAATTTTCAGTTGAGTGGTGCTGCTGCGGATCACCGCATACG‑GACCGGT‑GCCGATGGGATGGCTGGC‑AAAGTGA‑CTGATG‑TTTTccc < 2:516099/300‑1 (MQ=255) gTCCCTGACTTGCTGANTTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGCGACCGGTNGCCGATGGGATGGCTGGCNAAAGTTA‑CTGAgcggtt > 1:574417/1‑295 (MQ=255) gTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GACCGGT‑GCCGATGGGATGGCTGGC‑AAAGTTA‑CTGAGG‑GTTTCCCa > 1:688566/1‑300 (MQ=255) gTCCCTGACTTGCTGA‑TTCGCCCAGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GACCGGT‑GCCGATGGGATGGCTGGC‑AAAGTTA‑CTGAGG‑GTTTCCCa > 1:688581/1‑300 (MQ=255) gTCCATGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACATTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GACCGGT‑GCCGATGGGATGGCTGGC‑AAAGTTA‑CTGAGG‑GTTTCCCa > 2:349771/1‑300 (MQ=255) a‑TTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GACCGGT‑GCCGATGGGATGGCTGGC‑AAAGTTA‑CTGAGG‑GTTTCCCATTCGCGCGGC‑AGGat < 2:382809/300‑1 (MQ=255) gggTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTACAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTCGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GACCGGT‑GCCGATGGGATGGCTGGC‑AAAGTTA‑CTGAGG‑GTTTCCCATTCGCGCGGC‑AGGATCATCGACGGAACtt > 2:297850/1‑300 (MQ=255) ggTGCGGCTGTCGAATNGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GACCGGT‑GCCGATGGGATGGCTGGC‑AAAGTTGGCTGAGGNGTTTCCCATTCGCGCGGCNAGGATCATCGcggggaa > 1:572390/1‑294 (MQ=255) gCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACG‑GACCGGT‑GCCGATGGGATGGCTGGC‑AAAGTTA‑CTGAGG‑GTTTCCCATTCGCGCGGC‑AGGATCATCGCCGGAACTTGCCCca > 1:360424/1‑299 (MQ=255) | TCTCTTTGATTTTCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGCATGATCCCGGCAATCACCCGATGCTCACTGTGATCCTGATAAAAGGTTAGGGTGAGGCTTTCCAGGCCAGCCGGTTTTTCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGA‑TTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACG‑GACCGGT‑GCCGATGGGATGGCTGGC‑AAAGTTA‑CTGAGG‑GTTTCCCATTCGCGCGGC‑AGGATCATCGCCGGAACTTGCCCCA > NC_000913/75988‑76572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |