Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	74,009	T→C	Q171Q (CAA→CAG)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	74,009	0	T	C	100.0%	209.2 / NA	60	Q171Q (CAA→CAG)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/29);  total (31/29)

CAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCGGGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCAGATTAAAAAACACATGGGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGC  >  NC_000913/73742‑74284                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                    cagcAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGc                                                                                                                                                                                                                                                     >  1:626846/4‑300 (MQ=255) cagcAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAACGTTTTCCAGTGc                                                                                                                                                                                                                                                     >  2:328602/4‑300 (MQ=255)        aCCAACCCTAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGTCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAg                                                                                                                                                                                                                                               <  2:270387/299‑1 (MQ=255)            acccGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCACGCTGGGCGGCAAGCTGAAGTTGTTCGACGGGAATGTTTTCCAGTGCCTGGAGtaat                                                                                                                                                                                                                                           >  2:397300/2‑299 (MQ=255)            acccGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCAATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGtaata                                                                                                                                                                                                                                          <  1:447367/299‑1 (MQ=255)                taGGCAACACACCATCTGGATCAGCGTCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAg                                                                                                                                                                                                                                               <  2:270492/290‑1 (MQ=255)                 aGGCAACACACCATCTGGATAAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGtaataa                                                                                                                                                                                                                                         <  1:72583/296‑1 (MQ=255)                   gcaacacaCCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGAGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCAAGCCGCGCATCCAAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAGTGTTTTCCAGTGCCTGGAGTACTAAGCGGCt                                                                                                                                                                                                                                   >  2:337058/5‑300 (MQ=255)                    caacacaCCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTc                                                                                                                                                                                                                                  >  2:403503/4‑300 (MQ=255)                        acacCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCAAGTGCCTGGAGTAATAAGCGGCTCGCCa                                                                                                                                                                                                                              >  2:587514/1‑300 (MQ=255)                        acacCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAACGCCTGATAGATTGCCAGCTCGATATTTGTTGCCTGCGGACCGCCGCCCCGCGATAGCACGGTGGCGAAGCTGGCGACACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTCGTTCGAAGGGAATGTTTTCCAGTGCCTGGAGta                                                                                                                                                                                                                                             >  2:587612/1‑285 (MQ=255)                           ccATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACTAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATc                                                                                                                                                                                                                            >  1:322144/1‑299 (MQ=255)                             aTCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTACCTCCGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCAATAGCACGGTAGCAAAGCTGGCGAAACAGAGCACAAAGATAAGTGCAGCAAACGGCGGGATTTGTCGCAGTAACCACGGCCATTCGACGAAGAGGAAAAAATGCCAGCCGCGCATCCAAAGCTGGGCGGCAAGCTGACGTTGTTCGCCAGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCa                                                                                                                                                                                                                              >  2:354955/1‑295 (MQ=255)                                      agcGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAATTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACTGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTACCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTaaaa                                                                                                                                                                                                                <  2:457846/300‑1 (MQ=255)                                                          ggaaGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCAGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAAt                                                                                                                                                                                            >  1:321002/4‑300 (MQ=255)                                                          ggCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAAt                                                                                                                                                                                            >  1:321050/1‑300 (MQ=255)                                                          ggCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCAGGGAATGTTTTCCAGTGCATGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGTAAAAt                                                                                                                                                                                            >  2:47222/1‑300 (MQ=255)                                                          ggCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCAGTAACCACGGCCATTCGAAGAAGCGGACAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTACAGTGCCTGGAATAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAAt                                                                                                                                                                                            >  1:321038/1‑300 (MQ=255)                                                              ggATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCt                                                                                                                                                                                        <  1:416554/300‑1 (MQ=255)                                                                aTCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCtt                                                                                                                                                                                       >  1:22927/1‑299 (MQ=255)                                                                 tCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGANAAGATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGAAGTTGTTCGCAGGGGATGTTTTCCAGTGTCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAACAATACGTGCGACAGCAAAATACCTTGc                                                                                                                                                                                     >  2:219073/1‑300 (MQ=255)                                                                       tCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGATCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCAAAGCTGGGCGGCAAGCTGATGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATAGGTAGATTAACAACCACGTGCGCCAGCAAAACACCTTGCAGGc                                                                                                                                                                                 >  2:673595/1‑298 (MQ=255)                                                                                 gcgcCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGaaa                                                                                                                                                                     >  1:193125/1‑300 (MQ=255)                                                                                   gcCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGAAGAAGCGGAAAAAATGCCAGCCGCGCANCCAAAGCTGGGCGGAAAGCTGACGTTGTCCGCCGGGCATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTCGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGaaaa                                                                                                                                                                    >  2:216952/1‑299 (MQ=255)                                                                                      tGATGGATTGCCAGCTCGAGAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGTCGAAGCTGGCGAAACAGAGCATAAAGAGAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACTGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCTGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGc                                                                                                                                                                         <  2:453548/291‑1 (MQ=255)                                                                                                 cAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCa                                                                                                                                                         >  2:578592/1‑296 (MQ=255)                                                                                                  agcgcGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGTCGAAGCTGGCGAAACAGAGCATAAAGATAAATGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTCCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCGCTCCAGAc                                                                                                                                                      <  2:608653/294‑1 (MQ=255)                                                                                                  aGCTCGATAGTGGTTGNCTGCGGACCGCCGCCCAGNGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAANAGGTCCACTCCAGAcc                                                                                                                                                     <  1:571239/299‑1 (MQ=255)                                                                                                  aGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGTGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGTTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGAcc                                                                                                                                                     <  1:461718/299‑1 (MQ=255)                                                                                                  aGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCTGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGAc                                                                                                                                                      <  1:558104/298‑1 (MQ=255)                                                                                                  aGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGAc                                                                                                                                                      <  1:213350/298‑1 (MQ=255)                                                                                                     tCGATAGTTGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGTCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGAc                                                                                                                                                      <  2:253401/295‑1 (MQ=255)                                                                                                      cGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCAGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGc                                                                                                                                                 >  1:260670/1‑299 (MQ=255)                                                                                                       gATAGTGGTTGCCTGCGGACCTCCGCCCAGCGATAACACGGTGGCGAAGCTGTCGAAACAGAGCATAAATATAACTGCAACAACCGTCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTGGTTCGCCTGGAATGTTTTCCAGTGCCTGGAGTAATAAGCAGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCg                                                                                                                                                <  2:367924/299‑1 (MQ=255)                                                                                                                  ccTGTGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGACGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTCAAAAATACGTGCGCCAGCCAAATCCCTTGCAGGACGTAGGGAGAAAAGGTCCACTACAGACCGAGTGATTGGCagcgt                                                                                                                                     >  2:132217/1‑296 (MQ=255)                                                                                                                  ccTGCGGACCGCCGCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCAAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCATGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGTGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAACAGGTCCACTCCAGACCGAGCGATTGGCAGAgt                                                                                                                                     >  2:131654/1‑299 (MQ=255)                                                                                                                          ccgccgCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCATGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTgccagc                                                                                                                            >  1:284550/1‑300 (MQ=255)                                                                                                                                   aGCGATAGCACGGGGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGccc                                                                                                                      <  2:569020/297‑1 (MQ=255)                                                                                                                                    gCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGTGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGc                                                                                                                  >  2:390543/1‑300 (MQ=255)                                                                                                                                             cGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCGGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCGGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGACAGAGTGTTGCAAGCCAGCCCTGGCGACCATaaa                                                                                                         >  2:606581/1‑298 (MQ=255)                                                                                                                                                      aGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCTGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATAGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGaa                                                                                                <  1:620117/300‑1 (MQ=255)                                                                                                                                                      aGCTGGCGAAACAGAGCATAAAGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGaa                                                                                                <  1:125828/300‑1 (MQ=255)                                                                                                                                                                           aGATAAGTGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGTAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCagca                                                                           >  1:451876/1‑297 (MQ=255)                                                                                                                                                                                            ggcggGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGTAAAAAATGCCAGCCGCGCATCCCAAGCTGTGCGGCAAGCTGACGTTGTTCGCCTGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCCGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTc                                                          <  2:23910/300‑1 (MQ=255)                                                                                                                                                                                            ggcggGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCNCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGt                                                           <  2:213026/299‑1 (MQ=255)                                                                                                                                                                                            ggcggGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAgg                                                            <  1:480763/298‑1 (MQ=255)                                                                                                                                                                                               gggATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCAt                                                        <  1:468024/299‑1 (MQ=255)                                                                                                                                                                                                 gattttgcGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCAGCCCAAGCTGGGCGGCAAGCTGACATTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGc                                                     <  2:300042/293‑1 (MQ=255)                                                                                                                                                                                                 gATTTTTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGTGCATCCCAAGCTGGGCTGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACATGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGc                                                     <  2:62933/300‑1 (MQ=255)                                                                                                                                                                                                         gCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGAc                                             >  1:638648/1‑300 (MQ=255)                                                                                                                                                                                                              aaCCACAGCCACTCGACGAAACAAAAAAAATGCCAGCAGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGCTTTCCAGTGCCTGGAGTAATAAGCAGCTCGCAATCGGTAGATTAAAACATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGACTGGCAAAGCGCTGCCAGCAAGCCCTGGCGACAATAGACACTAAGAACACCGAAAACAGcaaacagc                                                                            >  2:18344/1‑257 (MQ=255)                                                                                                                                                                                                               aCCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAAACAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACa                                       >  2:552580/1‑300 (MQ=255)                                                                                                                                                                                                                    ggCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAgcgc                                   <  1:121446/299‑1 (MQ=255)                                                                                                                                                                                                                     gCCATTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGTCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTTGAGTAATAAGCGGCTCGCCATCGGTAGATGAAAAAATACGTGCGCCATCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAgcgc                                   <  2:397974/298‑1 (MQ=255)                                                                                                                                                                                                                        aTTCGACGAAGCGGAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGcc                              <  1:177420/300‑1 (MQ=255)                                                                                                                                                                                                                                     gAAAAAATGCCAGCCGCGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAgc                  >  1:230194/1‑299 (MQ=255)                                                                                                                                                                                                                                             gccagccgcGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCTGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGTGCCTGCGAt         <  1:446856/292‑1 (MQ=255)                                                                                                                                                                                                                                             gccagccgcGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGAt         <  1:446782/292‑1 (MQ=255)                                                                                                                                                                                                                                                 gccgcGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGAATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCAACCAGCACCGGGAGGATTAAGGTCATTGCGCACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATagag     <  1:663720/296‑1 (MQ=255)                                                                                                                                                                                                                                                    gcGCATCCCAAGCTGGGCGGCAAGCTGACGTTGTTCGCCGGGGATGTTTTACAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGTAGATTAAAAAATACGTGCGCCAGCAAAATACCTTGCAGGCAGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGAAGATAAGAATGCCGAAAACAGCAACAAGCACCGGGAGGATTAAGGTCATTGCGCACCGACGCAACAGCGCCCGCCGACCCGGACAGCACATGCGATAGAgcgc  >  2:198071/2‑300 (MQ=255)                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                    CAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCACGGTGGCGAAGCTGGCGAAACAGAGCATAAAGATAAGCGCAGCAACCGGCGGGATTTGTCGCCGTAACCACGGCCATTCGACGAAGCGGAAAAAATGCCAGCTACGCATCCCAAGCTGGGCGGCAAGTTGACGCTGTTCGCCGGGGATGTTTTCCAGTGCCTGGAGTAATAAGCGGCTCGCCATCGGCAGATTAAAAAACACATGGGCCAGCAAAATACCTTGCAGGCCGTAGGGCGAAAAGGTCCACTCCAGACCGAGCGATTGGCAGAGTGTTGCCAGCCAGCCCTGGCGACCATAGACGCTAAGAATGCCGAAAACAGCGACCAACACCGGGAGGATCAAGGTCATTGCACACAGACGCAACAGCGCCAGCCGACCCGGAAAGCGCCTGCGATAGAGCGC  >  NC_000913/73742‑74284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑