Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,604	T→C	L306L (TTA→TTG)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,604	0	T	C	100.0%	167.4 / NA	47	L306L (TTA→TTG)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/20);  total (27/20)

GATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGAT‑CACCGCCAGTAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCAC  >  NC_000913/73314‑73882                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                        gATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGANGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGg‑c                                                                                                                                                                                                                                                                               >  2:546316/1‑300 (MQ=255)     gcggCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGg‑cagca                                                                                                                                                                                                                                                                           <  2:580623/298‑1 (MQ=255)               aatggtgtTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGAAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAAcagcagc                                                                                                                                                                                                                                                                   <  1:399275/294‑1 (MQ=255)                 tggtgtTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAgcg                                                                                                                                                                                                                                                               <  1:168227/298‑1 (MQ=255)                   gtgtTGTTGAGCAGTANAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCAGCGACGATNCACCGCCAGCAACGGTGGNCAGCAAcagcagggagc                                                                                                                                                                                                                                                               >  1:574829/1‑295 (MQ=255)                                 tAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGNAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAacac                                                                                                                                                                                                                                               <  2:502307/300‑1 (MQ=255)                                 tAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAacac                                                                                                                                                                                                                                               <  1:288685/300‑1 (MQ=255)                                             ggTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCaa                                                                                                                                                                                                                                     <  1:199281/298‑1 (MQ=255)                                                                     ggCGAGGATCGACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCAAAGCACCNGTTGTGCCAGCACTTCCGGCAAATGGCGATTTAGCCCATCGACGAT‑CAACGCAAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCGGCACAATTAACACCGTGTAGCAGATGCGGATATGCAGACGATCGTcc                                                                                                                                                                                                             >  2:213350/1‑298 (MQ=255)                                                                     ggCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAAGTCCACAGCGCCTGCCACAGCACCAGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CAACGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCATAATTAACACCGTGTAGCAAATGCGGATATGCAGACGATCGTcc                                                                                                                                                                                                             >  2:558104/1‑298 (MQ=255)                                                                           gATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CAGCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGTAGACGATCGTCCGGGTcgc                                                                                                                                                                                                      >  2:447367/1‑299 (MQ=255)                                                                                           aTCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGcagcag                                                                                                                                                                                      <  1:49952/299‑1 (MQ=255)                                                                                             ctcCAGCGCCTGACCCGCCAGTATTTTCTGCCGCGCCAGAAGTTCGCGACTACTCCATAGCCGCATCATGGTAAGCACTACGCACAATACACCTGCCGACAGCGCAATACGCAACGAGGTCCACAGCGCCTGCAACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCGATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCAAGcaca                                                                                                                                                                                                                                                       >  2:72583/1‑232 (MQ=255)                                                                                                                      ttCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCTTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTc                                                                                                                                                          <  2:570426/300‑1 (MQ=255)                                                                                                                                               tGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCaacc                                                                                                                                 <  1:546316/300‑4 (MQ=255)                                                                                                                                                gCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTCACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACCCAATCgattactccgccacaccaaacc                                                                                                                                >  2:7901/1‑279 (MQ=255)                                                                                                                                                 cTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCaaacc                                                                                                                                >  1:453548/1‑295 (MQ=255)                                                                                                                                                 cTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTACGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAAccc                                                                                                                                >  2:480763/1‑299 (MQ=255)                                                                                                                                                   ccATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAAccc                                                                                                                                >  1:457846/1‑297 (MQ=255)                                                                                                                                                           gcaTCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CCTCCACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAAcaca                                                                                                                     >  1:270387/1‑300 (MQ=255)                                                                                                                                                           gcaTCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAAcaca                                                                                                                     >  1:270492/1‑300 (MQ=255)                                                                                                                                                             atcatGGTCAGTACTACGCACCATACACCTGCCGCCAGCGCAATACGCAACGAAGTCCACAGCGCCTACCACAGCACCGGTTGTGCCAGCACTTACGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCACCAGCCACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCATGTCGCAAATGCGGCTATGTAGACAACCGTACGGGTCGAGCCAGCCTTGCAACAGCGTGGTGCCTGGAGCCATGGCCTTAATCAACCGCCGACTCAGCAGCAACAACCCGAGgcacaacccc                                                                                                                   >  2:461718/1‑293 (MQ=255)                                                                                                                                                                  ggTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCTAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACCCACCATCt                                                                                                               >  2:468024/1‑299 (MQ=255)                                                                                                                                                                  ggTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCt                                                                                                               >  1:23910/1‑299 (MQ=255)                                                                                                                                                                      aGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGatc                                                                                                          <  2:651372/300‑3 (MQ=255)                                                                                                                                                                      aGCACTACGCACAATACACCCGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCTGCAACTGGCGATTTAGCCCATCGGCGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCTTGTCGCAAATGCGGCTATGCAGACAATCGTCCGGTTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGatc                                                                                                          <  2:65902/300‑3 (MQ=255)                                                                                                                                                                       gCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGatca                                                                                                         >  1:367924/1‑297 (MQ=255)                                                                                                                                                                                 caATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAgtat                                                                                               >  2:416554/1‑297 (MQ=255)                                                                                                                                                                                                  gcgcAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGAt                                                                              <  1:164137/300‑1 (MQ=255)                                                                                                                                                                                                        tACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCTAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGt                                                                        <  2:650394/300‑1 (MQ=255)                                                                                                                                                                                                         aCGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTc                                                                       >  2:571239/1‑300 (MQ=255)                                                                                                                                                                                                                   gTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCCAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGNAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAgc                                                             >  1:138194/1‑300 (MQ=255)                                                                                                                                                                                                                     ccACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAgcgc                                                           >  1:569020/1‑300 (MQ=255)                                                                                                                                                                                                                          gcgcCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGCCTCAGCAGCACAAACCCGAGGTAACACACCATCTGGATCAGCGCCAGCATCGCCGAGCGGGTAGGATCGTAGTCGTAACTAAGCGCCTGAt                                                      >  2:121446/1‑300 (MQ=255)                                                                                                                                                                                                                          gcgcCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGAACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCCTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCCATTGCTGACTCAGCAGCCCAAACCCGAGGCAACACACCATCTGGATCAGCGTCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAgtgc                                                           >  2:125828/1‑292 (MQ=255)                                                                                                                                                                                                                                      gCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCt                                          >  1:397974/1‑300 (MQ=255)                                                                                                                                                                                                                                               gtgCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGt                                   >  1:253401/1‑298 (MQ=255)                                                                                                                                                                                                                                               gtgCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTgg                                 <  2:262819/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gCCAGCACTTCCGGCAACTGTCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGtt                               <  2:390416/300‑2 (MQ=255)                                                                                                                                                                                                                                                 gCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAACTCGATAGTGGtt                               <  1:7901/300‑2 (MQ=255)                                                                                                                                                                                                                                                    aGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGcc                            <  1:369768/300‑1 (MQ=255)                                                                                                                                                                                                                                                        cTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGc                         >  2:383055/1‑299 (MQ=255)                                                                                                                                                                                                                                                         ttCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCgg                       <  2:589072/300‑1 (MQ=255)                                                                                                                                                                                                                                                          tCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAAAGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGAAAAACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGc                         >  2:620117/1‑297 (MQ=255)                                                                                                                                                                                                                                                            cGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGAcc                    >  1:681082/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         tttAGCCCATCTACGAT‑CACCATCAGCAACAGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAGCACCGTTTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCTAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCgg                       <  2:248187/284‑1 (MQ=255)                                                                                                                                                                                                                                                                                 aTCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGACCGCCGCCCAGCGATAGCAc  <  2:401063/297‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                        GATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGAT‑CACCGCCAGTAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACCGCCCCCCAGCGATAGCAC  >  NC_000913/73314‑73882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑