Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,582	C→G	V314L (GTA→CTA)	thiP ←	thiamine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,582	0	C	G	100.0%	162.9 / NA	46	V314L (GTA→CTA)	thiP	thiamine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (27/19);  total (27/19)

GATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGAT‑CACCGCCAGTAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACC  >  NC_000913/73314‑73864                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                             gATTGCGGCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGANGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGg‑c                                                                                                                                                                                                                                                             >  2:546316/1‑300 (MQ=255)     gcggCAGGCCAATGGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGg‑cagca                                                                                                                                                                                                                                                         <  2:580623/298‑1 (MQ=255)               aatggtgtTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGAAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAAcagcagc                                                                                                                                                                                                                                                 <  1:399275/294‑1 (MQ=255)                 tggtgtTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAgcg                                                                                                                                                                                                                                             <  1:168227/298‑1 (MQ=255)                   gtgtTGTTGAGCAGTANAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCAGCGACGATNCACCGCCAGCAACGGTGGNCAGCAAcagcagggagc                                                                                                                                                                                                                                             >  1:574829/1‑295 (MQ=255)                                 tAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGNAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAacac                                                                                                                                                                                                                             <  2:502307/300‑1 (MQ=255)                                 tAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAacac                                                                                                                                                                                                                             <  1:288685/300‑1 (MQ=255)                                             ggTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCaa                                                                                                                                                                                                                   <  1:199281/298‑1 (MQ=255)                                                                     ggCGAGGATCGACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCAAAGCACCNGTTGTGCCAGCACTTCCGGCAAATGGCGATTTAGCCCATCGACGAT‑CAACGCAAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCGGCACAATTAACACCGTGTAGCAGATGCGGATATGCAGACGATCGTcc                                                                                                                                                                                           >  2:213350/1‑298 (MQ=255)                                                                     ggCGAGGATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAAGTCCACAGCGCCTGCCACAGCACCAGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CAACGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCATAATTAACACCGTGTAGCAAATGCGGATATGCAGACGATCGTcc                                                                                                                                                                                           >  2:558104/1‑298 (MQ=255)                                                                           gATCAACATGCCGCTCATCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CAGCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGTAGACGATCGTCCGGGTcgc                                                                                                                                                                                    >  2:447367/1‑299 (MQ=255)                                                                                           aTCTCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGcagcag                                                                                                                                                                    <  1:49952/299‑1 (MQ=255)                                                                                             ctcCAGCGCCTGACCCGCCAGTATTTTCTGCCGCGCCAGAAGTTCGCGACTACTCCATAGCCGCATCATGGTAAGCACTACGCACAATACACCTGCCGACAGCGCAATACGCAACGAGGTCCACAGCGCCTGCAACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCGATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCAAGcaca                                                                                                                                                                                                                                     >  2:72583/1‑232 (MQ=255)                                                                                                                      ttCTGCCGCGCCCGAAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCTTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTc                                                                                                                                        <  2:570426/300‑1 (MQ=255)                                                                                                                                               tGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCaacc                                                                                                               <  1:546316/300‑4 (MQ=255)                                                                                                                                                gCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTCACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACCCAATCgattactccgccacaccaaacc                                                                                                              >  2:7901/1‑279 (MQ=255)                                                                                                                                                 cTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCaaacc                                                                                                              >  1:453548/1‑295 (MQ=255)                                                                                                                                                 cTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTACGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAAccc                                                                                                              >  2:480763/1‑299 (MQ=255)                                                                                                                                                   ccATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAAccc                                                                                                              >  1:457846/1‑297 (MQ=255)                                                                                                                                                           gcaTCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CCTCCACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAAcaca                                                                                                   >  1:270387/1‑300 (MQ=255)                                                                                                                                                           gcaTCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAAcaca                                                                                                   >  1:270492/1‑300 (MQ=255)                                                                                                                                                             atcatGGTCAGTACTACGCACCATACACCTGCCGCCAGCGCAATACGCAACGAAGTCCACAGCGCCTACCACAGCACCGGTTGTGCCAGCACTTACGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCACCAGCCACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCATGTCGCAAATGCGGCTATGTAGACAACCGTACGGGTCGAGCCAGCCTTGCAACAGCGTGGTGCCTGGAGCCATGGCCTTAATCAACCGCCGACTCAGCAGCAACAACCCGAGgcacaacccc                                                                                                 >  2:461718/1‑293 (MQ=255)                                                                                                                                                                  ggTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCTAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATTGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACCCACCATCt                                                                                             >  2:468024/1‑299 (MQ=255)                                                                                                                                                                  ggTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCt                                                                                             >  1:23910/1‑299 (MQ=255)                                                                                                                                                                      aGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGatc                                                                                        <  2:651372/300‑3 (MQ=255)                                                                                                                                                                      aGCACTACGCACAATACACCCGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCTGCAACTGGCGATTTAGCCCATCGGCGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCTTGTCGCAAATGCGGCTATGCAGACAATCGTCCGGTTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGatc                                                                                        <  2:65902/300‑3 (MQ=255)                                                                                                                                                                       gCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGatca                                                                                       >  1:367924/1‑297 (MQ=255)                                                                                                                                                                                 caATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAgtat                                                                             >  2:416554/1‑297 (MQ=255)                                                                                                                                                                                                  gcgcAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGAt                                                            <  1:164137/300‑1 (MQ=255)                                                                                                                                                                                                        tACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCTAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGt                                                      <  2:650394/300‑1 (MQ=255)                                                                                                                                                                                                         aCGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTc                                                     >  2:571239/1‑300 (MQ=255)                                                                                                                                                                                                                   gTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCCAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGNAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAgc                                           >  1:138194/1‑300 (MQ=255)                                                                                                                                                                                                                     ccACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAgcgc                                         >  1:569020/1‑300 (MQ=255)                                                                                                                                                                                                                          gcgcCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGCCTCAGCAGCACAAACCCGAGGTAACACACCATCTGGATCAGCGCCAGCATCGCCGAGCGGGTAGGATCGTAGTCGTAACTAAGCGCCTGAt                                    >  2:121446/1‑300 (MQ=255)                                                                                                                                                                                                                          gcgcCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGAACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCCTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCCATTGCTGACTCAGCAGCCCAAACCCGAGGCAACACACCATCTGGATCAGCGTCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAgtgc                                         >  2:125828/1‑292 (MQ=255)                                                                                                                                                                                                                                      gCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCt                        >  1:397974/1‑300 (MQ=255)                                                                                                                                                                                                                                               gtgCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGt                 >  1:253401/1‑298 (MQ=255)                                                                                                                                                                                                                                               gtgCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTgg               <  2:262819/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gCCAGCACTTCCGGCAACTGTCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGtt             <  2:390416/300‑2 (MQ=255)                                                                                                                                                                                                                                                 gCCAGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAACTCGATAGTGGtt             <  1:7901/300‑2 (MQ=255)                                                                                                                                                                                                                                                    aGCACTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGcc          <  1:369768/300‑1 (MQ=255)                                                                                                                                                                                                                                                        cTTCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGc       >  2:383055/1‑299 (MQ=255)                                                                                                                                                                                                                                                         ttCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCgg     <  2:589072/300‑1 (MQ=255)                                                                                                                                                                                                                                                          tCCGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAAAGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGAAAAACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGc       >  2:620117/1‑297 (MQ=255)                                                                                                                                                                                                                                                            cGGCAACTGGCGATTTAGCCCATCGACGAT‑CACCGCCAGCAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCGAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCGGAcc  >  1:681082/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         tttAGCCCATCTACGAT‑CACCATCAGCAACAGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAGCACCGTTTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCTGGCGCAATGGCCTTACTCAATCGCTGACTCAGCAGCACCAACCCTAGGCAACACACCATCTGGATCAGCGCCAGCATCGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTTGCCTGCgg     <  2:248187/284‑1 (MQ=255)                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                             GATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAGCCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTTCCGGCAACTGGCGATTTACCCCATCGACGAT‑CACCGCCAGTAACGGTGG‑CAGCAACAGCAGCAGCGCCAGCACAATTAACACCGTGTCGCAAATGCGGCTATGCAGACGATCGTCCGGGTCGCGCCAGCCTTGCAGCAGCGTGGTGCCGGGCGCAATGGCCTTACTCAATCGCTGACTCAACAGCACCAGCCCGAGGCAGCACACCATCTGGAGCAGCGCCAGCATTGCCGCGCGGGCAGGATCGTAGTCGTAACTCAGCGCCTGATAGATTGCCAGCTCGATAGTGGTCGCCTGCGGACC  >  NC_000913/73314‑73864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑