Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,752	T→A	G99G (GGA→GGT)	araB ←	ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,752	0	T	A	100.0%	188.8 / NA	57	G99G (GGA→GGT)	araB	ribulokinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (25/32);  total (25/32)

GGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTT‑CTT‑CAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATC  >  NC_000913/69472‑70027                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                    gggTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGt                                                                                                                                                                                                                                                                   >  1:161742/1‑299 (MQ=255)     ggtACCGGAAAGCAGAACTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGAAGATTGCGCCACGGTGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACAGCCAATATAGAGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTA‑GCTT‑CTTTCAACCGCAATGTAGTCTCTCCACAACACGAACATCGCGTTCGGGTTTCCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGt                                                                                                                                                                                                                                                                   >  2:298426/1‑295 (MQ=255)              aatcaaagCTGGCCCCCCATCGCCCAGCTCAATCCCCGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCCGGATTTTTGCCCAGACCCATTCGCTGGACTAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCCCAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCNCAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGcc                                                                                                                                                                                                                                                     >  2:540000/7‑300 (MQ=255)                   tagCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCGGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGGGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCACGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTAc                                                                                                                                                                                                                                                <  2:437223/299‑1 (MQ=255)                     gCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACt                                                                                                                                                                                                                                               >  1:369070/1‑299 (MQ=255)                        ggCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCACTCGGTGCGGGCGTCGCGCCGGTACTGTCa                                                                                                                                                                                                                                           >  2:372753/1‑300 (MQ=255)                        ggCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCa                                                                                                                                                                                                                                           <  1:174277/300‑1 (MQ=255)                              cAGTCGCACAGCTCAANCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCNATCGGTGCGGGCGTCGAGNCGGTACTGTCAACGCCa                                                                                                                                                                                                                                     >  1:587547/1‑300 (MQ=255)                                                 aCGATGCTGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTgcgc                                                                                                                                                                                                                  <  2:554535/300‑1 (MQ=255)                                                 aCGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTgcgc                                                                                                                                                                                                                  <  2:572572/300‑1 (MQ=255)                                                             aTTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACCCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGc                                                                                                                                                                                                      <  2:603209/300‑1 (MQ=255)                                                                        gcgcTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCCGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTTGACGCTAAGct                                                                                                                                                                                                >  2:101702/1‑295 (MQ=255)                                                                            tGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCa                                                                                                                                                                                       <  1:101325/300‑1 (MQ=255)                                                                             gTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCAc                                                                                                                                                                                      >  2:667571/1‑300 (MQ=255)                                                                             gTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGACGTCGAGCCGGTACTGTCAACGCAAATCCCGACCACAGCTACGCGCTGTTCGACGCTAAGCTCTGCAAGCa                                                                                                                                                                                       >  2:147974/1‑299 (MQ=255)                                                                                       gTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGt                                                                                                                                                                            >  1:75805/1‑300 (MQ=255)                                                                                          acaTGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCTTGGCACAAACGGTTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGc                                                                                                                                                                          <  1:104505/299‑1 (MQ=255)                                                                                                  gATTTTTGCCCAGAACCATTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GATTATTT‑CAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCAt                                                                                                                                                                 <  1:514668/300‑1 (MQ=255)                                                                                                                   aTTCGCTGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCa                                                                                                                                                 <  1:53934/299‑1 (MQ=255)                                                                                                                         tGGAATAAATACCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGgat                                                                                                                                          <  2:400608/300‑1 (MQ=255)                                                                                                                               aaataCCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGAc                                                                                                                                    <  1:281314/300‑1 (MQ=255)                                                                                                                                 ataCCGCCAATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACgg                                                                                                                                  <  1:112927/300‑1 (MQ=255)                                                                                                                                     cgccaATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACt                                                                                                                              >  2:654065/3‑300 (MQ=255)                                                                                                                                      gccaATATAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAAc                                                                                                                               <  1:367053/297‑1 (MQ=255)                                                                                                                                             tAGCGGGAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTc                                                                                                                      <  1:458427/300‑1 (MQ=255)                                                                                                                                                  ggAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGc                                                                                                                 >  1:112678/1‑300 (MQ=255)                                                                                                                                                   gAGTAGTCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGNTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGc                                                                                                                 <  2:226190/299‑1 (MQ=255)                                                                                                                                                       agtCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGNTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATcac                                                                                                            <  2:493381/300‑1 (MQ=255)                                                                                                                                                       agtCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCAAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCACTGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCAta                                                                                                              >  2:278585/1‑297 (MQ=255)                                                                                                                                                       agtCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCAGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATcac                                                                                                            >  2:278599/1‑300 (MQ=255)                                                                                                                                                       agtCAACATTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTA‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTTGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGAAGGAACTGGTTATTCGGGGCATca                                                                                                             >  2:280332/1‑299 (MQ=255)                                                                                                                                                            acattGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAAt                                                                                                       >  2:589683/4‑300 (MQ=255)                                                                                                                                                            acattGCCCGGCGCGTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAAt                                                                                                       >  1:154328/4‑300 (MQ=255)                                                                                                                                                                        gcgTGGCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGc                                                                                           >  1:531158/1‑300 (MQ=255)                                                                                                                                                                             gCACAAACGGGTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAAc                                                                                       >  1:484069/1‑299 (MQ=255)                                                                                                                                                                                     gggTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATAcc                                                                              <  1:382791/300‑1 (MQ=255)                                                                                                                                                                                       ttAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCAc                                                                            <  1:219881/299‑1 (MQ=255)                                                                                                                                                                                       gTAATCTCTTC‑GCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCAc                                                                            <  1:220264/300‑1 (MQ=255)                                                                                                                                                                                                ttcGCTT‑CTTTCAACCNCAGTGTGGTCTTTCCACANTACGAACATCGCGTTAGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGNGGATACCACTCTACGc                                                                     <  1:588957/297‑1 (MQ=255)                                                                                                                                                                                                ttcGCTT‑CTTTCAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCTGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGc                                                                     <  2:611922/297‑1 (MQ=255)                                                                                                                                                                                                 ttcgcttcctgcAACCGCAGTGTGTTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGc                                                                     <  2:68501/288‑1 (MQ=255)                                                                                                                                                                                                         tt‑cAACCGCAGTTTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGAt                                                           <  1:280332/300‑1 (MQ=255)                                                                                                                                                                                                         tt‑cAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGAt                                                           >  2:580727/1‑300 (MQ=255)                                                                                                                                                                                                         tt‑cAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGAt                                                           <  1:278599/300‑1 (MQ=255)                                                                                                                                                                                                         tt‑cAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCGGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGAt                                                           <  1:278585/300‑1 (MQ=255)                                                                                                                                                                                                              aCCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCTGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCTTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATTTAGTCACTCGGATGATGACGGAACTGGTTATTCGTGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGcc                                                                    <  2:367279/287‑2 (MQ=255)                                                                                                                                                                                                                   aGTGTGGTCTTTCCACAATACGAACATCGCTTTCTGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGTGGATACCACTCTACGCTGGTGGCGATCTCTTCAc                                                   <  2:34419/299‑1 (MQ=255)                                                                                                                                                                                                                   aGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCAcc                                                  >  2:442554/1‑300 (MQ=255)                                                                                                                                                                                                                    gtgtGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTNCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGAAGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCAcc                                                  >  2:503981/1‑299 (MQ=255)                                                                                                                                                                                                                     tgtgGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGNATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCgc                                                <  1:133246/300‑2 (MQ=255)                                                                                                                                                                                                                                 acaATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCTTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTGCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTcc                                     <  1:375955/299‑1 (MQ=255)                                                                                                                                                                                                                                 acaATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTcc                                     <  2:587547/299‑1 (MQ=255)                                                                                                                                                                                                                                 acaATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCa                                    >  1:622899/1‑300 (MQ=255)                                                                                                                                                                                                                                     tACGAACATCGCGTTCGAGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTGCTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGTGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGc                                <  2:370155/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  aCTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTGTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAAt   >  2:412351/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   cTCCGGGCGCAGCGCCAGCACGTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCAACGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCaaactc  >  2:128378/1‑297 (MQ=255)                                                                                                                                                                                                                                                                    tCCGGGCGCAGCGCCAGCACTTTACCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTTTGCCAACGTGGATACCACTCTACGCTGGTGGCAATCTCTTCACCGCTGGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATc  <  2:161742/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                    GGGTGGTACCGGAAAGCAGAGCTGGCACCCAGTCGCACAGCTCAATCCACGATGCGGCAGATTGCGCCACGGCGCTGTCCTGGCGAGTCACATGCAGGATTTTTGCCCAGAACCATTCGCTGGAATAAATACCACCAATGTAGCGGGAGTAGTCAACGTTGCCCGGCGCGTGGCACAAACGGGTAATCTCTTCCGCTT‑CTT‑CAACCGCAGTGTGGTCTTTCCACAATACGAACATCGCGTTCGGGTTTTCGGCAAACTCCGGGCGCAGCGCCAGCACGTTTCCGTCGGCATCAATCGGTGCGGGCGTCGAGCCGGTACTGTCAACGCCAATCCCGACCACAGCTGCGCGCTGTTCGACGCTAAGCTCTGCAAGCACGGTTTTCAGTGCCGCTTCCATTGACTCAATGTAGTCACGCGGATGATGACGGAACTGGTTATTCGGGGCATCACAAAATTGCCCTTTCTGCCAACGGGGATACCACTCTACGCTGGTGGCGATCTCTTCACCGGTAGCGCAGTCCACCGCCAAAGCTCGCACAGAATCACTGCCAAAATC  >  NC_000913/69472‑70027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑