Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,466,438	0	T	G	25.0%	91.6 / 28.5	53	pseudogene (1047/2518 nt)	ydbA	putative outer membrane protein, N‑terminal fragment
Reads supporting (aligned to +/- strand):  ref base T (22/17);  new base G (7/6);  total (30/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTC  >  NC_000913/1466143‑1466736                                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                            tGATTCAGGACGGCGATCTGGATGTCAGCGGCTGCGTTTACGGTATTGATATCACCGTTGACAGCTCGACGGTGGATAACAAGGGCACCATGACCATCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTc                                                                                                                                                                                                                                                                                                         <  1:205012/300‑1 (MQ=255) tGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTc                                                                                                                                                                                                                                                                                                         <  1:759003/300‑1 (MQ=255)                aTCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCAACGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCAACAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑CC‑GGCAAGGATTCCA‑CCAGCAAGGAAATC‑AACGGCAATAAAGGAAAAGTTATTCAGGACGGCGATCTgg                                                                                                                                                                                                                                                                                          >  1:362452/1‑300 (MQ=18)                                           gTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACTCAGATCAACGGTAATGACGCCACGGCGAATAACAACGGCAAAACCACCGTTG‑AT‑GGCAAAGATTCCA‑CAGGTACAGAAATC‑GAGGGTAATAACGGAAAGGTAAATCAGGACGGTGGCCTGGATGTCAgaggcagagatcatatcacca                                                                                                                                                                                                                                                               >  1:741311/1‑284 (MQ=17)                                             aTTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCGCCATCACCAACGGTGGCA‑CCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATT‑GCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGat                                                                                                                                                                                                                                                             >  1:232185/1‑300 (MQ=11)                                              ttGATATCACCGGTGACAGCGCGACGGTGGATAACAATGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGata                                                                                                                                                                                                                                                            <  1:28569/300‑1 (MQ=255)                                              ttGATATCACCGGTGACAGCGCGACGGTGGATAACAATGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGAGCGACGGTGACGAGGCCATCGTCAAGAACGAAGGCGAGA‑GCATCAGCACCAACGGTGGCA‑CCGGCACCCGTATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAGGGATTCCA‑CCGGTACGGAAATT‑GCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGata                                                                                                                                                                                                                                                            <  1:98390/300‑1 (MQ=11)                                              ttGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGata                                                                                                                                                                                                                                                            <  1:300479/300‑1 (MQ=255)                                                    tCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGACGGCGAGC‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATCAATGGTGCTGACGCCGCGGCAAACCAAACCGGCAAAACCACCGTTG‑AC‑GGAAAGGATTCCA‑CCGGTACGGAAATT‑GCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGTGGTCACGGTATTAATATCACCg                                                                                                                                                                                                                                                      >  2:243188/1‑300 (MQ=14)                                                      aCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATT‑GCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGc                                                                                                                                                                                                                                                    >  1:527135/1‑300 (MQ=11)                                                      aCCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACACAGATTAATGGTGATGACGCCACGGCNAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑ACCGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCAACGGc                                                                                                                                                                                                                                                    >  2:215273/1‑300 (MQ=255)                                                         ggTGACAGCTCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGAc                                                                                                                                                                                                                                                 <  1:750172/300‑1 (MQ=255)                                                                gcgcGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGACACCCAGACTAATGGTGCTGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑CC‑GGTAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACGGCGCaa                                                                                                                                                                                                                                          >  2:114030/1‑300 (MQ=255)                                                                        gTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATACCGACGGC‑ACACGCACCATCAACAACGGTGGCA‑CCGGCAACCNGATTAATGGTGCTGACACCACAGCCCCCAACAACAGCAAGACCACCATTG‑AA‑GGCAAGGATNCAA‑AAGGTACGGAAATC‑AACGGTAATAAAGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACAGTGGAt                                                                                                                                                                                                                                  >  2:695231/1‑300 (MQ=18)                                                                        gTGGATAACAAGGGCACCATGACCGGCACCGATCCGGAGTCCAGGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGTAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAt                                                                                                                                                                                                                                  <  1:785261/300‑1 (MQ=255)                                                                           gATAACAAGGGCACCATGACCGGCACCGATCCGGAGGCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGACACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AAc                                                                                                                                                                                                                              <  1:715383/300‑1 (MQ=255)                                                                                        ccATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATT‑GCTGGCAATAACGGGAAGGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGa                                                                                                                                                                                                                 >  1:743063/1‑300 (MQ=11)                                                                                        ccATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATCAACGGTAATGACGCCACGGCGAATAACAACGGCAAAACCTATGTTG‑CA‑TACACATCTCCAA‑ACAG‑CCAAAAACCGATCGGTCATAGAGGACGAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACGAGGGCACCATGa                                                                                                                                                                                                                 >  1:307860/1‑300 (MQ=12)                                                                                         cATGACCGGCACCGATCCGGAGTCCATGGGTATCCAGATCGACGTTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGTAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGAc                                                                                                                                                                                                                <  1:556451/300‑1 (MQ=255)                                                                                                  cACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACCAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCAATAGCCAGCC‑GGCTAACGGTGATGACGCCGCGGTACACAAAAACGGCAAAACCAACGTCG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAAGT‑GAGGGCAATAGGGGGAAGGTGGTTAAGGATGGCGAGCTGGATGTCCGCGGCGGCGGTCAAGGTGTTGATGTTACGGGTGACAGGGCAACGGTGGATCAACA‑GGGCACCATGACCGTCGCCgg                                                                                                                                                                                                       >  2:464172/1‑299 (MQ=255)                                                                                                         gacaggTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAg                                                                                                                                                                                                <  1:161878/295‑1 (MQ=255)                                                                                                         ccGGAGTCCATGGGTATCCATATCTACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGGTGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAg                                                                                                                                                                                                <  1:868201/300‑1 (MQ=255)                                                                                                          cGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATT‑GCTGGCAATAACGGGAAGGTGATGCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGt                                                                                                                                                                                               <  2:517395/300‑1 (MQ=11)                                                                                                              gTCCATGGGTATCCAGATCGACGGTGACCAGGCCATCGTCAATAACGCAGGCACGA‑GCCCCATCACCAAGGGTGGCA‑CCGGCCAACAGCGCAACAGTGATAACACCAAGGCAAACCCCAACGGCNAAACCACCATTA‑AC‑AGCAAGGATTCCN‑CCGGTAAGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGTGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCAGGAGTccct                                                                                                                                                                                           >  2:593661/1‑298 (MQ=17)                                                                                                              gTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AA‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCCCCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCAACGATCCagagctgtc                                                                                                                                                                                           >  2:161007/1‑295 (MQ=255)                                                                                                                 gctagatgtgtataatagacaggACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCTGCACCCAGATTAATGTTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCgg                                                                                                                                                                                        <  2:262487/278‑1 (MQ=17)                                                                                                                     ggTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGCGGCA‑CCGGCACCCAGATTAACGGTAACGACTACCCATCACACA‑CCTCT‑AAGAACCCCCAAGGCACAGTCACGGATTTAA‑CAGGTGAGGAGATC‑AACGGTAATAACGGAGGAGTGGTTAGGGAAGGAGGTCTGGATGTCGGTGGCGGCGGTCACGGTATTGATATCAACGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACAATGACCGTCACCGATCCGGAGTCCATCGGTATc                                                                                                                                                                                    >  1:387778/1‑300 (MQ=9)                                                                                                                           cAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGtt                                                                                                                                                                              <  1:710873/300‑1 (MQ=255)                                                                                                                                     gTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAACGGTGATGACGCCACAGCCCACAACCATGTCAAAACCACTATTA‑AC‑GG‑AAAGACTCCAACCGATCCTAACCTC‑GACAGCCCTAACGGACCATTTCTTCAGTACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGNGCACCATGACCGTCAACGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGAct                                                                                                                                                                    >  2:653661/1‑299 (MQ=9)                                                                                                                                       gACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCTGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCggctgtctcttatacacatctccg                                                                                                                                                                  >  1:264166/1‑278 (MQ=255)                                                                                                                                             gCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTCCGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGgtt                                                                                                                                                            >  1:322734/1‑300 (MQ=255)                                                                                                                                                   gTCAAGAACGGAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTaac                                                                                                                                                      <  1:661300/300‑1 (MQ=255)                                                                                                                                                     cAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTaacaa                                                                                                                                                    <  1:506548/300‑1 (MQ=255)                                                                                                                                                        tAACGAAGGCGAGA‑GCACCATCACCAACGGTNGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑NACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCAACGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGGGTCCATCGGTATCCAGGTTGACGGCGACCAGGccatcgtcaataacga                                                                                                                                                 >  2:604439/1‑285 (MQ=255)                                                                                                                                                                            aTCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCAAAGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCAACGATCAGGAGTCCATCGGTATCCAGGTTGACGGAGAACAGACGGTTGTCAATAACGAAGGCGAGAgcgctattact                                                                                                                              >  2:390807/1‑293 (MQ=17)                                                                                                                                                                                  taatggtggcactggaatccgtaTTAATGGTAATGACGCCACAGCAAACAACAACGGCAAAACCACCGTCG‑AC‑TGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAAGAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCATCGGCGGCGGTCACGTTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCTGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACgg                                                                                                                         <  2:38929/278‑1 (MQ=18)                                                                                                                                                                                             ccggcaccCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCAGTTGTCAATAACGAAGGCGAGAGCACCATCACCAACGGTGGCACCGGCa                                                                                                              >  1:665488/1‑300 (MQ=255)                                                                                                                                                                                                   cacAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGAACGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCCGGCAGTTGTTAACAACGAAGGCGAGAGCGCCATCAACAACGGCGGCACCGGCACGCAGa                                                                                                        >  2:376499/3‑300 (MQ=255)                                                                                                                                                                                                       gATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGNCCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTaa                                                                                                    >  1:639185/1‑300 (MQ=255)                                                                                                                                                                                                       gATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCACTATCAACAATGGTGGCACCGGCACTCAGAtcaa                                                                                                    >  2:133565/1‑297 (MQ=255)                                                                                                                                                                                                                 gatgaCGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGAc                                                                                          >  1:290952/1‑300 (MQ=255)                                                                                                                                                                                                                      cGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGccgc                                                                                     >  1:128756/1‑298 (MQ=255)                                                                                                                                                                                                                        ccACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACgg                                                                                   >  1:387259/1‑300 (MQ=255)                                                                                                                                                                                                                                aacaacaacGGCAAAACCACCGTTG‑AC‑GGTAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGGAAAGTGATTCAGGACGGCGAGCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGAGGACGCCACGGCAaacaac                                                                           <  1:653780/300‑1 (MQ=255)                                                                                                                                                                                                                                     caacGGCAAAACCACCGTTG‑AC‑GGCAAAGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACgg                                                                      <  2:422859/300‑1 (MQ=255)                                                                                                                                                                                                                                                        g‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCAACGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGc                                                   >  2:476843/1‑300 (MQ=255)                                                                                                                                                                                                                                                               taaagATTCCA‑CCGGTACGGAAATC‑AGCGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTTACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGa                                              <  2:78081/296‑1 (MQ=255)                                                                                                                                                                                                                                                                  ggATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTc                                           >  1:247946/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     ttCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCAc                                        <  1:386912/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       ccA‑CCGGTACGGAAATC‑AACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCg                                      <  1:702058/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     aaTC‑AACGGTAATAACGGGAAAGTGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGGTTGACGGTGACCAGGCAGTTGTCAATAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGGAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTga                         >  2:523605/1‑299 (MQ=11)                                                                                                                                                                                                                                                                                                     cGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGATTGACGGCGACCAGGCGATCGTCAACAACGAAGGCGAGAGCACCATCACCAACGGCGGCACCGGCACTCAGATTACCGGTAACGACGCCACAGCAAACAACAACGGAAAAACCACCGTTGACGGAAAAGATTCCACAGGTACCGAAATTGCTGGCAATACCggggc          >  2:296903/1‑298 (MQ=11)                                                                                                                                                                                                                                                                                                           aGTTATTCAGGACGGCGATCTGGATGTCAGTGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTCGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAGGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGAt    <  2:26971/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                             ttATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACCAAAATCGCGGGCAATATCggcattgtaaatc  <  1:483694/300‑12 (MQ=17)                                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                            TGATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGTGACAGCGCGACGGTGGATAACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATGGGTATCCAGATCGACGGTGACAAGGCCATCGTCAATAACGAAGGCGAGA‑GCACCATCACCAACGGTGGCA‑CCGGCACCCAGATTAATGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTG‑AC‑GGCAAGGATTCCA‑CCGGTACGGAAATC‑AACGGTAATAACGGAAAAGTTATTCAGGACGGCGATCTGGATGTCAGCGGCGGCGGTCACGGTATTGATATCACCGGCGACAGCGCAACGGTGGAT‑AACAAGGGCACCATGACCGTCACCGATCCGGAGTCCATCGGTATCCAGGTTGACGGCGACCAGGCGGTTGTTAACAACGAAGGCGAGAGCGCCATCACCAACGGTGGCACCGGCACGCAGATTAACGGTGATGACGCCACGGCAAACAACAACGGCAAAACCACCGTTGACGGCAAGGATTCCACCGGTACGGAAATTGCTGGCAATAACGGGAAGGTGATTC  >  NC_000913/1466143‑1466736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑