Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	339,958	G→A	D64N (GAC→AAC)	yahH →	putative uncharacterized protein YahH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	339,958	0	G	A	100.0%	106.8 / NA	38	D64N (GAC→AAC)	yahH	putative uncharacterized protein YahH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/23);  total (15/23)

CGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGC‑CGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGG  >  NC_000913/339670‑340246                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                 cgGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATcc                                                                                                                                                                                                                                                                                       <  1:87615/300‑1 (MQ=255)           gCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGNCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACTAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAg                                                                                                                                                                                                                                                                             <  2:844018/300‑1 (MQ=255)               ccACCTTTTGCCTGCTTAGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCGCCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAAGAATGGCGCAAGAACCTGTAGGCATGATAAGCGTAGCGAATCAGGNATTTGTAACCATTGC‑CGGATGCGGCGTGAACGCCCTATCCGGcatcagcgtggc                                                                                                                                                                                                                                                                         >  2:724336/1‑289 (MQ=18)                  ccTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGcgcg                                                                                                                                                                                                                                                                      >  1:292170/1‑300 (MQ=255)                            gCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCAACATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAg                                                                                                                                                                                                                                                           >  1:86902/1‑300 (MQ=255)                                    aaaGCCATTCTTGGCTGGGGCGAACGTTACGGCTTTTGACCTGCCTGTTCATTCCGGATGCGGCGTGAACGCCTTATCCTGCCTACGAATGGCGCAAGGATCTGTAGGCCTGATAAGCGTAGCGCAGCAGGCATTGGTCACTATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTAAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATa                                                                                                                                                                                                                                                   <  2:183032/300‑1 (MQ=14)                                      aGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCTGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAg                                                                                                                                                                                                                                                 <  1:157769/300‑1 (MQ=255)                                      aGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAg                                                                                                                                                                                                                                                 <  1:862514/300‑1 (MQ=255)                                         cATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGt                                                                                                                                                                                                                                              <  1:198730/300‑1 (MQ=255)                                          aTTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTa                                                                                                                                                                                                                                             >  1:267125/1‑300 (MQ=255)                                                     gTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGNCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGANTCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGc                                                                                                                                                                                                                                  <  2:780079/300‑1 (MQ=255)                                                     gTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCCGGc                                                                                                                                                                                                                                  >  1:485127/1‑300 (MQ=255)                                                     gTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGc                                                                                                                                                                                                                                  >  1:480719/1‑300 (MQ=255)                                                              ttACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGCCGCGCATCAGGCATTTGTCAACATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGGGGAT‑TGTAGGCCTAATAAGCGTAGCGCATTAGGCagtctggcg                                                                                                                                                                                                                         >  2:586138/1‑292 (MQ=11)                                                               tACGGCGTCTGACCTATATGTTCGTGCCGGATGCGGCGTGGACGCCTTATCCTGCCTACGAATGGCGCAAGAATCTGGAGGCGTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCagtctggcgt                                                                                                                                                                                                                        <  2:505789/300‑10 (MQ=11)                                                               tACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCTGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCagtctggcgt                                                                                                                                                                                                                        <  1:971174/300‑10 (MQ=11)                                                               tACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCagtctggcgt                                                                                                                                                                                                                        <  1:128172/300‑10 (MQ=255)                                                                   gCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAAT‑TGTAGGCCTGATAAGCGTAGCGCATCAGGCagtctggcgttggt                                                                                                                                                                                                                    <  1:347306/300‑14 (MQ=11)                                                                                                                                                                       acctacatgttcatGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTNGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAAcc                                                                                                                 >  1:696500/14‑300 (MQ=11)                                                                                                                                                                            catgttcatGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGTGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATc                                                                                                            >  1:486583/9‑300 (MQ=11)                                                                                                                                                                                ttcatgctggATGCGGCGTGAACGCCTTATCCTGCCTACGGATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAccc                                                                                                        <  2:343491/292‑1 (MQ=11)                                                                                                                                                                                     gtcGGATGCGGCGTGAACGCCTTATCCGACCTACGAATGGCGCAAGGATCTTTAGGCCTGATAAGCGTATCGCATCAGGCGTTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGt                                                                                                   <  2:369448/298‑1 (MQ=14)                                                                                                                                                                                          aTGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTCTGGCGTTGGTCATAACCCCATCACCctctttatctg                                                                                              <  2:865977/300‑8 (MQ=11)                                                                                                                                                                                          aTGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCgg                                                                                              <  1:223320/300‑1 (MQ=255)                                                                                                                                                                                            gcggcgTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGAc                                                                                            <  1:377565/300‑1 (MQ=255)                                                                                                                                                                                            gaggcgTGAACGCCTTAGCTTGCTTATGAATGGCTCGAGAATCTGTATGCCTGATAAGCTTAGCGCATCAGGCATTCTCCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGTGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGTCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCACATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGAc                                                                                            <  2:97093/298‑1 (MQ=17)                                                                                                                                                                                                 gTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATaac                                                                                       <  1:778547/300‑1 (MQ=255)                                                                                                                                                                                                                  ccTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAACCTCTGTAGCGGACATAACAACATGAAAGAGCTtgt                                                                      >  1:478867/1‑300 (MQ=255)                                                                                                                                                                                                                     aCGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGt                                                                   <  1:211974/300‑1 (MQ=255)                                                                                                                                                                                                                                      cTGTAGGCCTGATAAACGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATCTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAAACCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCa                                                  >  1:441287/1‑300 (MQ=18)                                                                                                                                                                                                                                           ggCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGCCGTTGGTCATAACCCCATCAACCTCTGNAGCGGACATAAAAACATGGAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGc                                             >  2:708163/1‑300 (MQ=17)                                                                                                                                                                                                                                                  tAAGCGTAGCGCATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCa                                      <  1:39419/300‑1 (MQ=255)                                                                                                                                                                                                                                                       gTAGCGCATCAGGCATTTGGCGCCA‑TCCTCGTATGCGGCGTGAACGCTTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCCTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCCGTAGCGGACATAACAACATGGAAGAACTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGac                                 <  2:91949/300‑2 (MQ=18)                                                                                                                                                                                                                                                             cATCAGGCATTTGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTTTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTTTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACgcc                           <  1:974859/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      tttGTCACCATTGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCCTCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTCGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGATATAACAACATGAAAGAGCGTGTGGTCGTTGCCATTGGTGGCAACAGCATTCTCAAAGATAACGCCAGCAAGTca                  >  2:591337/1‑299 (MQ=21)                                                                                                                                                                                                                                                                             ccattGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGGATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCAACCTATGTAGCGGACATAGCAACATGAAAGAGCTTGTGGTAGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCACGCCAGTCGATTGAGc           >  2:582096/5‑300 (MQ=21)                                                                                                                                                                                                                                                                               attGC‑CGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAGCCAGTCGATTGAGCAt         <  1:526691/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                       gATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCNATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCaggcgg  >  1:679790/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                 CGGGCATTGTGCATCCACCTTTTGCCTGCTTCGAGAAAGCCATTCTTGGCTGGTGCGAACGTTACGGCGTCTGACCTACATGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAATGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGCCACCACTGC‑CGGATGCGGCGTGGACGCCTTATCCGGCCTACGAGTGGCGCGAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTTGTCACCATTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGAGTGGCGCGAGAATTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCCTCTGTAGCGGACATAACAACATGAAAGAGCTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGCGG  >  NC_000913/339670‑340246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑