Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,396,772 T→C F236F (TTT→TTC)  amiB → N‑acetylmuramoyl‑L‑alanine amidase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,396,7720TC98.8% 279.6 / NA 81F236F (TTT→TTC) amiBN‑acetylmuramoyl‑L‑alanine amidase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (32/48);  minor base A (0/1);  total (32/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGATTTACAGTTC  >  NC_000913/4396484‑4397066
                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                      
gAAACGCGTTGAAACACCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGtttt                                                                                                                                                                                                                                                                                             <  2:458551/300‑1 (MQ=255)
    cgcgTTTAAACGCTTGTGGTTGTCGCACCGCGCTTCAGCGAACCTGCGTGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGTGTGTTAGCAGCAGTAATACGGTAACGCGTCCGGCAGCACACACGACGGCTAACACTGGCGATAAAATCATCATCGCTATTGATGCCGGACAC‑GGCAGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGTAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAAcc                                                                                                                                                                                                                                                                                         <  2:125863/300‑1 (MQ=255)
    cgcgTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAAcc                                                                                                                                                                                                                                                                                         <  1:162175/300‑1 (MQ=255)
     gcgTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAAcca                                                                                                                                                                                                                                                                                        >  2:299927/1‑299 (MQ=255)
                     ggTTTTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTTGCGATAANATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGTAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACtt                                                                                                                                                                                                                                                                        <  2:189736/300‑1 (MQ=255)
                      gTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGNTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACttt                                                                                                                                                                                                                                                                       >  1:573524/1‑300 (MQ=255)
                      gTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACttt                                                                                                                                                                                                                                                                       >  1:145110/1‑300 (MQ=255)
                      gTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACttt                                                                                                                                                                                                                                                                       >  2:315008/1‑300 (MQ=255)
                      gTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAACATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACttt                                                                                                                                                                                                                                                                       >  1:387191/1‑300 (MQ=255)
                          gcGCACCGCGCGTCAGCGAACCGGCACGCAGTCCGTTTAAAACGGAAAGTAACCGCACGACGGGTGTGATCAGCAGTAAGATGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATCATCATCGCTATTGATGCCGGACAC‑GGCAGTCAGGACCCCGGCGCTATCGGCCTCGGGGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACCTTGCTC‑AACGACGATCCGATGTTCAAATGCGTTTTAACCCGTGACGGGGATTACTTTAtct                                                                                                                                                                                                                                                                   <  2:340965/299‑1 (MQ=255)
                           cGCACCGCGTGTCAGCGAACCGGCGCGCAATCCATTTGAAACGTAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGTATAAAAATGTCATCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTGACCCGTGACGGGGATTACTTTAtctc                                                                                                                                                                                                                                                                  <  2:323050/300‑1 (MQ=255)
                                    cgTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGCTGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATggg                                                                                                                                                                                                                                                         >  1:679098/1‑300 (MQ=255)
                                     ttcagctgccccgcgctcagtCCGTTTAAAACGGAAAGTAACCGCACTAAGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGTGACGCGTGCAACGGCTAATACTGGCGATAAAACCATCACCACTATTGACACCGTACAC‑GGCGGTCAGGACCCCGGCGCTATCGTCCCCAGTCGTACCCGGGAGAAAAGTGTCACCATCTCTAGTGCGCGTAACCTGCGCACTCTGTTC‑AATGACGATCCGATGTTCAAAGGCGTCTTAACCCGTGACGGGGATTACGGTATCTGGGGGATgggg                                                                                                                                                                                                                                                        <  2:225368/280‑1 (MQ=255)
                                           gAACCGGCGCGCAATCCGTTTAAAACGTAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGt                                                                                                                                                                                                                                                  <  1:132287/300‑1 (MQ=255)
                                           gAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGt                                                                                                                                                                                                                                                  <  1:132456/300‑1 (MQ=255)
                                             aCCGGCGCGCAATCCGTGTAAAACGAAAAATAACCGCACTACGGGTGTGATCAGCAGTAATACGGTAACGCGTCCTGCAGCGCGCGCGACGGCTAACACTGTCGGTAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTTGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AACTACGATCCGATGTTCAAAGTCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGa                                                                                                                                                                                                                                                <  2:388031/300‑1 (MQ=255)
                                                tgcgcgtagtCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGGAAGACGGTAACGCGTCCGGCAGTACGCGCGACGGCTAACACTGGCGATAAAACTATCACCGCTATTGATGCCGGACAC‑GGCAGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGTAGAAAAATGTCACCACCGCCATCGCGCGTAAAGTGCGTACTTTGCTC‑ACTGACGATCCGATGTTCAACTTCTTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGAtgt                                                                                                                                                                                                                                             <  2:351394/291‑1 (MQ=255)
                                                  cgcgcAGTCCTTTTAAAACGGAAAATAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTGACGTTGGCGATAAAGTGATCATCGCTATTGATGCCGGATAC‑GGCGGTCAGTACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTGCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTgg                                                                                                                                                                                                                                           <  2:351516/300‑1 (MQ=255)
                                                  cgcgcAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTgg                                                                                                                                                                                                                                           <  1:95644/300‑1 (MQ=255)
                                                     acaattcggttAAAACGGAAAGGAACCGCACTACGGGGGTTATCAGCGGGAATACAGGAGAGCGTCCGGCAGTGCGCGCGACGGATAACACTTGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCCGGCAAGACCCTGGCGCTAGCGGCCCCGGTGGTACGCGGGAGAAAACTGTCACCATTGCCATCGCGCGTAAATTGCGTGCTCTGCTC‑AACCACGATCCGATGTCCAAATTCGTTTTAACCCTTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCAc                                                                                                                                                                                                                                        <  2:422702/291‑1 (MQ=255)
                                                         tCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTaa                                                                                                                                                                                                                                    <  1:406520/300‑1 (MQ=255)
                                                                                   cTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAAAGCCAATTTCCTCGTGTCGa                                                                                                                                                                                                          >  2:334260/1‑300 (MQ=255)
                                                                                        ggTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGGTGGGGGGCAGCGATGCGGGAAGTAAGGAAAAGGAAAATTTATTCGTGTCGAtttag                                                                                                                                                                                                     >  2:17470/1‑297 (MQ=255)
                                                                                        ggTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCAc                                                                                                                                                                                                     >  1:666086/1‑300 (MQ=255)
                                                                                         gtgtTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGTGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACg                                                                                                                                                                                                    >  1:168704/1‑300 (MQ=255)
                                                                                          tgtTATCAGCATTAATACGGCAACGCGTCCGGTAGCGCACGCGACGGCTAACACTGGCGATAAAACTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTTGCGCTATCTGCCCCGTTGGTACGCGGGAGAAAAATGTCACCATCGCTATCGCGCGTAAGTTGCGTATTTTGCTC‑AATGACGATCCGATGTTCAAATGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGc                                                                                                                                                                                                   <  2:24083/300‑1 (MQ=255)
                                                                                          tgtTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGc                                                                                                                                                                                                   <  1:835273/300‑1 (MQ=255)
                                                                                           gtTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCt                                                                                                                                                                                                  >  1:168987/1‑300 (MQ=255)
                                                                                           gtTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTAGGTGGTGGGGAGCAGTGATGTGGCACGTAAGAAAAACGCCAATTTCCTCGTGTGGATTCACGCt                                                                                                                                                                                                  >  2:449933/1‑300 (MQ=255)
                                                                                             tATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGa                                                                                                                                                                                                >  2:399489/1‑300 (MQ=255)
                                                                                                cagcagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGc                                                                                                                                                                                             <  1:766074/300‑1 (MQ=255)
                                                                                                  gcggTAATGCGCTAACGCGCCCGGCAACACTCGAGACGGCTAACACTGACAATAGAGTTATCATCGATATTGGGGACGGACAC‑GGCGGTCAGGACCCTGGCGCTGTCGGCCCCGGTGGTGCGCGGTAGAAAAATGGCACCACCACCATCGCGCGTGAATTGCGTA‑TTTGGTCCAAGTACGATCCGATGTTCAAATGCGTTTTAACCCGGGACGGGGATTACTTTACCTCGGTGATTGGGCGCATTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCg                                                                                                                                                                                           <  2:177986/297‑1 (MQ=255)
                                                                                                   cagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGGTGGGGCGCAGTGGTGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGc                                                                                                                                                                                          >  2:784265/1‑300 (MQ=255)
                                                                                                   cagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAANTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGNTGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGcagc                                                                                                                                                                                          >  2:767796/1‑297 (MQ=255)
                                                                                                     gTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTAGTGTTGATTCACGCTGATGCAGCAc                                                                                                                                                                                        >  2:496305/1‑300 (MQ=255)
                                                                                                     ctaatgcgtcaacgactCCGGCATCGCGCCCGACCGCGAACCCCGTCGATAAAACCATCATCGCTATTTATGCCGGACACTATAGGT‑AGGACCCTATCGCTGTCGGCCTCGTGGGTACCCGTTAGAAAGGTGTCAACATCGCCATCGCGCGTAAATTGCGGACCTTGCGC‑AACGACGCTCCGATGTTCAAATTCGTTTGAACCCGTGACGGGGATTACTTTATCTCTGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGGGTCGATTCACGCTGATGCCGCAc                                                                                                                                                                                        <  2:474582/284‑1 (MQ=255)
                                                                                                             gTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTANGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGc                                                                                                                                                                                <  1:564545/300‑1 (MQ=255)
                                                                                                             gTAACGCGTCCGGCAGCGCGCGCGACGGCGAACACTGGCAATAAAATTATAATCGCGATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTNGTACGCGGGAGAAAAATGCCACCATCGCCATCACGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCATTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGc                                                                                                                                                                                <  2:741079/300‑1 (MQ=255)
                                                                                                               aaCGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAg                                                                                                                                                                              <  1:24251/300‑1 (MQ=255)
                                                                                                                    gTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCTCAAGGGTGTGGAACGTAAGCAANACGCCAATTTCCTCGTGTCGATTTAAGATGATGCCGCAGCGAACAGCNGTGCGa                                                                                                                                                                         >  2:689202/1‑300 (MQ=255)
                                                                                                                                   cgACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTAt                                                                                                                                                          <  1:28726/300‑1 (MQ=255)
                                                                                                                                       ggCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGt                                                                                                                                                      >  1:495911/1‑300 (MQ=255)
                                                                                                                                       ggCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGAGCAGTGATGTGGAACGTAGGCAAAACGAAAATTTCCTAGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGt                                                                                                                                                      >  2:154952/1‑300 (MQ=255)
                                                                                                                                                 gttgATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGNGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAAc                                                                                                                                            <  2:722507/297‑1 (MQ=255)
                                                                                                                                                  tagagaaaatcaccatcGCTATTGGGGACGGACAC‑GGCAGGCAGGACCCCGGCGCTAGCGGCCCCGTTGGTACGCGGGAGAAAAAGGTCACCAGCGCCATCGCGCGTAAATTACGTACTTTGCGC‑AATGACAATCCGAGGTTCAAAGGCGTTTGAACCCGGGACGGGGATCACTTTACCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAAcc                                                                                                                                           <  2:707250/287‑1 (MQ=255)
                                                                                                                                                  gCGATAAGATTAGCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATTTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGTTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAAcc                                                                                                                                           <  2:480461/300‑1 (MQ=255)
                                                                                                                                                  gCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAAcc                                                                                                                                           <  1:402438/300‑1 (MQ=255)
                                                                                                                                                   cGATAAAATTATCATCACTATTGACGCCGGACAC‑GGCGGTCATTACCCTGGCGCTATCGGCCCCGGGGGTACGCGGGAGGAAAATGTCACCATCGCCAGCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCNTTTTAACCCGTGACGGGGATTACTTTNTCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCg                                                                                                                                          <  2:564458/300‑1 (MQ=255)
                                                                                                                                                                      aTTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATg                                                                                                                       >  2:462547/1‑300 (MQ=255)
                                                                                                                                                                         gATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGcc                                                                                                                    <  1:726848/300‑1 (MQ=255)
                                                                                                                                                                            gCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTAGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAgc                                                                                                                 >  1:271030/1‑300 (MQ=255)
                                                                                                                                                                                   ac‑GGCGTTCAGGACCCTTGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCACCAGCGCGCGTAGATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGNAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGNCGCGCCAACAGTGAAATGGCCAGctggctgg                                                                                                          <  2:594099/300‑1 (MQ=255)
                                                                                                                                                                                       gcggTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGagc                                                                                                       <  1:12552/300‑1 (MQ=255)
                                                                                                                                                                                         ggTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGACATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGCGCTGCGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGCATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCAAGCGGGCTGGagcag                                                                                                     >  2:339533/1‑300 (MQ=255)
                                                                                                                                                                                              ggACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACga                                                                                                >  1:375870/1‑300 (MQ=255)
                                                                                                                                                                                              ggACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGNCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACga                                                                                                >  1:556710/1‑300 (MQ=255)
                                                                                                                                                                                                 cccTGGCGCTATCTGCCCCGTTGGGACGCGGGAGAAAAATGTCACCATCGCCATNGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTNCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGaa                                                                                             <  2:762963/300‑1 (MQ=255)
                                                                                                                                                                                                 cccTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTAGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGaa                                                                                             >  2:747219/1‑300 (MQ=255)
                                                                                                                                                                                                      gcgcTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGt                                                                                        >  2:521206/1‑300 (MQ=255)
                                                                                                                                                                                                          tATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGTGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTAGTGTCGATTCACGCTGATGCAGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTTTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGa                                                                                    >  2:70756/1‑300 (MQ=255)
                                                                                                                                                                                                          tATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTAGGTGATGGAGGGCCTTGGGGTGGAACGTAAGGAAAATGACANTTTCCTAGTGTCGATTAACGTTGATGCAGCACCGAACAGCAGTGTGACTGGCGCGTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAGTGGCCCGTTGGCTGGAGCAGCACGAGAAAccgtagaa                                                                                    >  2:203206/1‑293 (MQ=255)
                                                                                                                                                                                                                 cccGGTGTTATGCGGGAGAAAAATGTCACCATTACCATCGCGCGTAGAGTGCGTACTTTGCTC‑AATGCTGATTCGGTGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCGCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTTTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGctgctg                                                                             <  2:231051/300‑1 (MQ=255)
                                                                                                                                                                                                                 cccGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGctgctg                                                                             <  1:686897/300‑1 (MQ=255)
                                                                                                                                                                                                                    ggtggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtgggt                                                                          <  1:131789/300‑1 (MQ=255)
                                                                                                                                                                                                                     gtggtCCGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtgggtg                                                                         >  1:227674/1‑300 (MQ=255)
                                                                                                                                                                                                                     gtggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtgggtg                                                                         >  1:337559/1‑300 (MQ=255)
                                                                                                                                                                                                                      tggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtgggtgg                                                                        <  1:815826/300‑1 (MQ=255)
                                                                                                                                                                                                                         tACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGc                                                                     <  1:658428/300‑1 (MQ=255)
                                                                                                                                                                                                                         ggcgcgGTAGAAAAATGGCACCATCGCCATCGCGCGTAAATTACGTACGTGGCGC‑AACGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGGAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGc                                                                     <  2:796143/298‑1 (MQ=255)
                                                                                                                                                                                                                               ggAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGa                                                               <  1:703809/300‑1 (MQ=255)
                                                                                                                                                                                                                                  gaAAAGTGTCACCAGCGCCATCGCGCGTAAATTGCATACTTTGCGC‑AGGGATGATCCGATGGTCAAAGGCGTTTTAACCCTTGACGGGGATTACTGTATCTCGTTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCACACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCGCTAACCGTCGCGCCAACATTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGGGAtgt                                                            <  2:247261/300‑1 (MQ=255)
                                                                                                                                                                                                                                                    catcgcGCTTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACATTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACagtcagtc                                          <  2:463518/300‑1 (MQ=255)
                                                                                                                                                                                                                                                     atcgcGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCt                                         <  1:344442/300‑1 (MQ=255)
                                                                                                                                                                                                                                                         cgcgTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCCGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATAGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGAcc                                     >  2:810936/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                    cGTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGc                          <  1:227638/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                     gTACTTTGCTC‑AATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGTTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGcc                         <  1:324217/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                     gTACGTTGCTC‑AATGACGATCCGATGTTCAAAGTCGTTGTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAATGGTGAAATGGCCAACTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTAATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGcc                         <  2:27588/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                      gtgtttggcttaaTGACGATCCGATGTTAAAATGCGTTTTAACCTCTGACGGGTATTAATTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCAATTCACGCTGATGCCACACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAATAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGcc                         <  2:122522/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    gACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGANTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGAt           <  1:571242/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        aTCCTATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCATTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTGTGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATTGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTAGTTAAGCCAGGCGGTGCTGGATGTAc       <  2:120088/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                             aTGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGATTTACAGTTc  >  1:43853/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                      
GAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACAC‑GGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTC‑AATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGATTTACAGTTC  >  NC_000913/4396484‑4397066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑