Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,691	T→A	L191Q (CTG→CAG)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,691	0	T	A	100.0%	345.1 / NA	96	L191Q (CTG→CAG)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (41/55);  total (41/55)

GTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCT‑‑‑GAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACG  >  NC_000913/3486402‑3486975                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                              gctgaaatttcgtacaaaaaatttcgccaattgattcaggtaaacccggacattcggatgcgtttgtctgcacagatggcgcttcgtctgcaagtcacttcatagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcaccctgacggtatgcaaatcaaaattacccgtcaggaaattgttcagattgtcggctgttctcgtgaaaccgtgggacgcattttaaT‑‑‑GAAGATGCAGTAAGATCAg                                                                                                                                                                                                                                                                                     <  2:305550‑M2/20‑1 (MQ=255)                   agatttngccaattgattcaggtaaacccggacatgctgatgcgtttgtctgcanagatggcgcttcgtctgcaagtcacttcagagaaagtgggcagcctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCacac                                                                                                                                                                                                                                                                   <  2:697534‑M2/38‑1 (MQ=255)                             aattgattcaggtaaacccggacattctgatgcgtttgtctgcacagatggcgcgtcgtctgcaagtcacttcatagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCa                                                                                                                                                                                                                                                         <  2:431250‑M2/48‑1 (MQ=255)                                 gattcangtaaacccggacattctgatgcatttgtctgcacagatggcgcgtcgnctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCAgcgt                                                                                                                                                                                                                                                     <  2:689582‑M2/52‑1 (MQ=255)                                      aggtaaacccggacattctgatgcgtttgtctgcacagatggcgcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGttt                                                                                                                                                                                                                                                >  1:736337‑M2/244‑300 (MQ=255)                                       ggtaaacccggacattctgatgcgtttgtctgcacagatggcgcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgnatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgncaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTa                                                                                                                                                                                                                                               >  2:666897‑M2/243‑300 (MQ=255)                                       ggtaaaaccggacattctgatgcgtttgtctgcacagatggcgcgtcgtctgcaagtcacttaagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgtacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaacttacccgtcagggaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctcaT‑‑‑GAAGATGCAGGAAGGTCAGAACCTGATCTCCGCACACGGTAAAaccatcgtcgctca                                                                                                                                                                                                                                               >  2:10974‑M2/243‑296 (MQ=255)                                         taaacccggacattctgatgcgtttgtctgcacagatggcgcgtcgtctgcaagtcacttcatagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACg                                                                                                                                                                                                                                             <  2:648540‑M2/60‑1 (MQ=255)                                               cggacattctgatgcgtttgtctgcacagatggcgcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcnaaacaaccagacgctatgactcacccngacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTc                                                                                                                                                                                                                                       >  2:568057‑M2/235‑300 (MQ=255)                                                  acattctgatgcgtttgtctgcacagatggcgcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGtt                                                                                                                                                                                                                                    <  2:476210‑M2/69‑1 (MQ=255)                                                    attctgatgcgtttgtctgcacagatggcgcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTaa                                                                                                                                                                                                                                  >  1:542670‑M2/230‑300 (MQ=255)                                                     ttctgatgcgtttgtctgcacagatggcgcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAAt                                                                                                                                                                                                                                 >  1:349018‑M2/229‑300 (MQ=255)                                                       ctgatgcgtttgtctgcacagatggcgcttcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATcc                                                                                                                                                                                                                               <  1:384099‑M2/74‑1 (MQ=255)                                                       ctgatgcgtttgtctgcacagatggcgcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtgagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATcc                                                                                                                                                                                                                               <  1:664883‑M2/74‑1 (MQ=255)                                                       ctgatgcgtttgtctgcacagatggcgcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATcc                                                                                                                                                                                                                               <  2:541293‑M2/74‑1 (MQ=255)                                                       ctgatgcgtttgtctgcacagatggcgcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATcc                                                                                                                                                                                                                               <  1:186078‑M2/74‑1 (MQ=255)                                                                ttgtctgcacagatggcgcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgtattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGt                                                                                                                                                                                                                      <  1:771507‑M2/83‑1 (MQ=255)                                                                 tgtctgcacagatggcgcgtcgtctgcaagtcacttcagcgaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTg                                                                                                                                                                                                                     >  1:448940‑M2/217‑300 (MQ=255)                                                                 tgtctgcacagatggcgcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTg                                                                                                                                                                                                                     >  1:13409‑M2/217‑300 (MQ=255)                                                                  gtctgcacagatggcgcgtcgtctgcaagtcactgcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTg‑g                                                                                                                                                                                                                   <  1:440822‑M2/85‑1 (MQ=255)                                                                     tgcacatatggcgcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATTAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑gcgc                                                                                                                                                                                                                <  2:61335‑M2/88‑1 (MQ=255)                                                                      gcacagatggcgcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑Gcgcg                                                                                                                                                                                                               >  1:54317‑M2/212‑300 (MQ=255)                                                                            atggcgcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgttggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCt                                                                                                                                                                                                         <  2:818784‑M2/95‑1 (MQ=255)                                                                            atggcgcgtcgtctgcaagtcacgtcagatacagtggtcaacctggtgttcctcgacttcacgtgccgcattgcacagactctgctgaatctggcaaaacaaccatacgctatgactcacccgggcggtatgcaaatcaaaactacccgtcaggaaattgttcagactgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GGAGGTGCAGGAAGATCAGAACCTAATCTCCGCACACGGTAAAACCATCTTCGTTTACTGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTaccc                                                                                                                                                                                                         <  2:450890‑M2/95‑2 (MQ=255)                                                                               gcgtgtcgtctgcgagtcacttcggagaaagtgggcaaccttgcgttcctcgacttgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCTTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGt                                                                                                                                                                                                      <  2:756608‑M2/98‑1 (MQ=255)                                                                               gcgcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacntgacgggccgcattgcacagactctgccgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGt                                                                                                                                                                                                      <  2:628943‑M2/98‑1 (MQ=255)                                                                                 gcgtcgtctgcaagtcacttcagagaaagtgggcaaccttgcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAg                                                                                                                                                                                                    <  2:344217‑M2/100‑1 (MQ=255)                                                                                 gcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgttggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAg                                                                                                                                                                                                    <  1:411423‑M2/100‑1 (MQ=255)                                                                                 gcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAg                                                                                                                                                                                                    <  1:122876‑M2/100‑1 (MQ=255)                                                                                 gcgtcgtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaactacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAg                                                                                                                                                                                                    <  2:798701‑M2/100‑1 (MQ=255)                                                                                      gtctgcaagtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAgcgcgc                                                                                                                                                                                               >  1:831738‑M2/196‑300 (MQ=255)                                                                                             agtcacttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattggacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTg                                                                                                                                                                                        >  2:527340‑M2/189‑300 (MQ=255)                                                                                              gtcacttcagagaaagtgggcaacctggcgttcctcgacgcgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAATCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGt                                                                                                                                                                                       <  2:61610‑M2/113‑1 (MQ=255)                                                                                                 acttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTc                                                                                                                                                                                    >  1:236577‑M2/185‑300 (MQ=255)                                                                                                  cttcagagaaagtgggcaacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCATTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTcc                                                                                                                                                                                   >  1:276444‑M2/184‑300 (MQ=255)                                                                                                                   aacctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACATGGTAGCGCGCCATCTTGTTTCCACCGATGTGGCGCAGAc                                                                                                                                                                  >  2:313130‑M2/167‑300 (MQ=255)                                                                                                                    acctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatcttgcaaaacaaccagacgctatgacccacccggacggtatgcaaattaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACt                                                                                                                                                                 <  2:411582‑M2/135‑1 (MQ=255)                                                                                                                    acctggcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACt                                                                                                                                                                 >  1:410282‑M2/166‑300 (MQ=255)                                                                                                                         gcgttcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGAttt                                                                                                                                                            <  1:5384‑M2/140‑1 (MQ=255)                                                                                                                            gtgTTCCTCGAAGTGGCGGGCCGCATTGCACAGACTTTGCTGAATCTGGTAAAACAACCAGATGCGATGACTCACCTGGGCGGTATGCAAAGTAAAATTACCCTTCAGGAGAGNGGTCAGATTGTCTGCTGTTCTCGTGAAACCGTTGTACGCATTCTAACGAAGATGCAGGAAGNTCAGAACCTGATCTCCGCACACGGTAAAACCATCGGCGTTTACGGCACTCGTTAATCCCTTCGGA‑TGTGCGCGTTACCTGGTAGTGCGCCATTTTGTTTCCCCCGGTGTGGCGCAGACTGAttt                                                                                                                                                            <  2:668220/298‑1 (MQ=255)                                                                                                                             tcctcgacgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCa                                                                                                                                                        >  2:1618‑M2/157‑300 (MQ=255)                                                                                                                                    cgtgacgggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGat                                                                                                                                                 <  1:300138‑M2/151‑1 (MQ=255)                                                                                                                                      tgacgggccgcattgcacagactctgctgaatctgtcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattgttcagattgtcggctgttcttgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCTTCGGAGTG‑GCGCGTTACCTGTTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGatat                                                                                                                                               <  1:490665‑M2/153‑1 (MQ=255)                                                                                                                                          gggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctattactcacccggacggtatgcaaatcaaaattacccgtcaggaagttgttcagattgtcggctgttctcgtgaaaccgttggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCTTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAAc                                                                                                                                           <  2:640750‑M2/157‑1 (MQ=255)                                                                                                                                          gggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAAc                                                                                                                                           <  1:383482‑M2/157‑1 (MQ=255)                                                                                                                                          gggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTAACTGGTAGCGCGCCATTTTGTTTACCCCGATGTGGCGCAGACTGACTTATCACCCCGATATCAAc                                                                                                                                           >  1:202111‑M2/144‑300 (MQ=255)                                                                                                                                          gggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCAGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAAc                                                                                                                                           <  1:52722‑M2/157‑1 (MQ=255)                                                                                                                                          gggccgcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagatngtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAAc                                                                                                                                           <  2:719711‑M2/157‑1 (MQ=255)                                                                                                                                               gcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGc                                                                                                                                      >  1:727251‑M2/139‑300 (MQ=255)                                                                                                                                               gcattgcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCACCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGc                                                                                                                                      >  2:821926‑M2/139‑300 (MQ=255)                                                                                                                                               gcagtgcacagactctgcttcctctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAATCTCCGAACCTGATCTCCTCACACGTTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGc                                                                                                                                      <  2:348826‑M2/162‑1 (MQ=255)                                                                                                                                                    gcacagactctgctgaatctggcaaaacaacnagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacncattctaaT‑‑‑GAAGATGCAGGAAGATCCGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTc                                                                                                                                 >  2:583617‑M2/134‑300 (MQ=255)                                                                                                                                                    gcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTNCGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTc                                                                                                                                 >  1:597823‑M2/134‑300 (MQ=255)                                                                                                                                                    gcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCCCCCCGATATCAACTATGCACTTc                                                                                                                                 >  1:383586‑M2/134‑300 (MQ=255)                                                                                                                                                    gcacagactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTc                                                                                                                                 >  1:250916‑M2/134‑300 (MQ=255)                                                                                                                                                         gactctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGTGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATTACTCCGATATCAACTATGCACTTCGACaa                                                                                                                            >  2:83494‑M2/129‑300 (MQ=255)                                                                                                                                                         gactctgctgaatctggcaaaacaaacagacgctatgacccacccggacggtatgcaaatcaaaattaccagtcaggaaattggtcagattgtcggctgttctcgtgaacccgtgggacgcattctaaT‑‑‑GAAGATGTAGGAAGATCAGAACCTGATCTCCGACCACGGTACAACCATCGTCGTTTACGACACTCGTTAATCCAGTCGGAGTG‑GCGCGTCAACTGGTAGCGCGCCATTTTGTTTACCCCGATGTGGCGCAGACTGATTTATCAACCAGATATCAACTATGCACTTAGACaa                                                                                                                            >  2:83013‑M2/129‑300 (MQ=255)                                                                                                                                                             ctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTAGGAGTG‑GCGCGTTACGTGGTAGCGCGCCATTTTGTTTCCCCCGNTGTGGCGCAGACTGATTTATCACCCAGATATCAACTATGCACTTCGACAAACGc                                                                                                                        >  2:614134‑M2/125‑300 (MQ=255)                                                                                                                                                             ctgctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCAGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGc                                                                                                                        >  2:631447‑M2/125‑300 (MQ=255)                                                                                                                                                               gctgaatctggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAAGCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGTGCCATTTTGTTTCCCCCGATNTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTg                                                                                                                      >  2:612772‑M2/123‑300 (MQ=255)                                                                                                                                                                        ggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgttaaaccgttggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGt                                                                                                             <  2:419635‑M2/187‑1 (MQ=255)                                                                                                                                                                        ggcaaaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGt                                                                                                             <  1:208644‑M2/187‑1 (MQ=255)                                                                                                                                                                            aaacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGc                                                                                                         >  1:395335‑M2/110‑300 (MQ=255)                                                                                                                                                                             aacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑NAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGcc                                                                                                        <  1:579749‑M2/192‑1 (MQ=255)                                                                                                                                                                             aacaaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGcc                                                                                                        <  1:140689‑M2/192‑1 (MQ=255)                                                                                                                                                                               caaccagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCg                                                                                                      <  2:812114‑M2/194‑1 (MQ=255)                                                                                                                                                                                   cagacgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGc                                                                                                  <  1:435416‑M2/198‑1 (MQ=255)                                                                                                                                                                                       cgctatgactcacccggacggtatgtaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAAACATCGTAGTTTACGGCACTCGTTAATCCAGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCAGATGTGGCGCAGACTGCTTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTACCCGTGGCCGtt                                                                                              >  2:107004‑M2/99‑300 (MQ=255)                                                                                                                                                                                       cgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACATGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACACACGCTGGTGCTATGTTTGCCCGTGAccgct                                                                                              >  2:143797‑M2/99‑298 (MQ=255)                                                                                                                                                                                       cgctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGtt                                                                                              >  1:437522‑M2/99‑300 (MQ=255)                                                                                                                                                                                        gctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaatcggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCAAGTTTGTTTCCCCCGATGTGGCGCAGAATGATTTATCACCCCGATATCCACTCTGCCCTTCGACAAACGCTGATGCTATGTTTGCCCGTGGCCGTTg                                                                                             >  2:113534‑M2/98‑300 (MQ=255)                                                                                                                                                                                         ctatgactcacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTgg                                                                                            >  1:426539‑M2/97‑300 (MQ=255)                                                                                                                                                                                                 cacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGANCCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGc                                                                                    <  1:560385‑M2/212‑1 (MQ=255)                                                                                                                                                                                                 cacccggacggtatgcaaatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGc                                                                                    <  1:22460‑M2/212‑1 (MQ=255)                                                                                                                                                                                                                  aatcaaaattacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTc                                                                   <  1:702509‑M2/229‑1 (MQ=255)                                                                                                                                                                                                                    tcaaaactacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCgg                                                                 <  1:49622‑M2/231‑1 (MQ=255)                                                                                                                                                                                                                           tacccgtcaggaaattggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGctc                                                          <  2:747991‑M2/238‑1 (MQ=255)                                                                                                                                                                                                                                         ttggtcagattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTcc                                            <  1:403655‑M2/252‑1 (MQ=255)                                                                                                                                                                                                                                            gtcagattgtctgctgtgctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCTTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTcctgc                                         <  2:158934‑M2/255‑1 (MQ=255)                                                                                                                                                                                                                                                gattgtcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGATGGTGTTGTGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGGGAATTACGATTTGGTCTGCTCTTCTACCTCGTTCCTACCTGt                                     >  2:345106‑M2/42‑300 (MQ=255)                                                                                                                                                                                                                                                 attgtcggctgttctagtgagaccgggggacgcattccaaT‑‑‑GAAGATGCAGGAAGACCAGAACCTGATCTCCGCACATGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCGTTCTGTTTCCACCGATGTGGCGCAGACTGATTTATCACCCAGATATCCACTATGTACCTCGACAAACGCTGGTGCTATGTTTGCACGTGGCTGTTGGGTTAATGCTCGGCGACTTACGATTCGGTCTGCCCTTCTCCCTCGTTCATGCCTGtt                                    >  2:319091‑M2/41‑300 (MQ=255)                                                                                                                                                                                                                                                     tcggctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGTTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCaa                                <  1:468278‑M2/264‑1 (MQ=255)                                                                                                                                                                                                                                                        gctgttctcgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAatat                             <  1:29550‑M2/267‑1 (MQ=255)                                                                                                                                                                                                                                                          tgttctngtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACNTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTg                           <  2:692946‑M2/269‑1 (MQ=255)                                                                                                                                                                                                                                                                cgtgaaaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGcc                     >  1:742649‑M2/26‑300 (MQ=255)                                                                                                                                                                                                                                                                     aaccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCNCAGACTGATTTATCACCCCGATATCANCTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGAt                <  2:569635‑M2/280‑1 (MQ=255)                                                                                                                                                                                                                                                                       ccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTNCCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATAc              <  1:605178‑M2/282‑1 (MQ=255)                                                                                                                                                                                                                                                                       ccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGNTATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCACGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTACCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATAc              >  2:183220‑M2/19‑300 (MQ=255)                                                                                                                                                                                                                                                                       ccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATAc              <  2:164254‑M2/282‑1 (MQ=255)                                                                                                                                                                                                                                                                       ccgtgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATCGCGGGCCTTGATAc              >  2:347367‑M2/19‑300 (MQ=255)                                                                                                                                                                                                                                                                       aagtgggacgcattataaT‑‑‑GAAGATGTAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCAGGTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGGGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTGGGCGAATTACGATTCGGTCTGCGCTTCTCCCGCGTTCCTGCCTGTTGCAATATTGCGGGCCgtgagac              <  2:460429‑M2/282‑4 (MQ=255)                                                                                                                                                                                                                                                                          tgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATNCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTNCCTGTTGCAATATTGCGGGCCTTGATACGcc           <  2:578984‑M2/285‑1 (MQ=255)                                                                                                                                                                                                                                                                          tgggacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGcc           <  1:673036‑M2/285‑1 (MQ=255)                                                                                                                                                                                                                                                                             gacgcattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCa        >  1:329503‑M2/13‑300 (MQ=255)                                                                                                                                                                                                                                                                                 cattctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCTTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATaaa    <  1:709613‑M2/292‑1 (MQ=255)                                                                                                                                                                                                                                                                                   ttctaaT‑‑‑GAAGATGCAGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTAATAAACg  >  2:532396‑M2/7‑300 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                              GTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCT‑‑‑GAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAATCCCGTCGGAGTG‑GCGCGTTACCTGGTAGCGCGCCATTTTGTTTCCCCCGATGTGGCGCAGACTGATTTATCACCCCGATATCAACTATGCACTTCGACAAACGCTGGTGCTATGTTTGCCCGTGGCCGTTGGGTTAATGCTTGGCGAATTACGATTCGGTCTGCTCTTCTCCCTCGTTCCTGCCTGTTGCAATATTGCGGGCCTTGATACGCCTCATAAACG  >  NC_000913/3486402‑3486975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑