Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	1,333,261	Δ8 bp	coding (2214‑2221/2598 nt)	topA →	DNA topoisomerase 1

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1333260	1 (0.010)	67 (0.640)	51/596	0.8	98.5%	coding (2213/2598 nt)	topA	DNA topoisomerase 1
	?	NC_000913	1333269 =	1 (0.010)					coding (2222/2598 nt)	topA	DNA topoisomerase 1

GGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAACACACGTAAGATTTTACGTAACGGCGAAGTGGCACCACCGAAAGAAGATCCGGTGCCATTACCTGAGCTGCCGTGCGAAAAATCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAG  >  NC_000913/1332961‑1333558                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                           ggAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTaaaaa                                                                                                                                                                                                                                                                                                            <  1:483417/300‑1 (MQ=255)           gTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGCCGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTAAAAATGGGGCGTTTCGGTAAATACATGACATGCACCAACGAAGAGTGTAAAAAagattttacg                                                                                                                                                                                                                                                                                                  >  2:46745‑M1/1‑290 (MQ=255)                             gtcgaAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCATGCACCAACGAAGAGTGTCAAAAagattttacgtagcggcgaagtggcacc                                                                                                                                                                                                                                                                                >  1:402951‑M1/3‑272 (MQ=255)                                      gCTGAAACCAACGCGCTGCGCGCAAAACGTCGGTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaaga                                                                                                                                                                                                                                                                       <  1:5098‑M1/300‑38 (MQ=255)                                                        cgcgcAAAACGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTTTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacc                                                                                                                                                                                                                                                     <  1:1948‑M1/300‑56 (MQ=255)                                                         gcgcAAAACGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCACGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGCGTGTAAAAAagattttacgtcacggcgaaatggcaccaccgaaagaagatccggtgccattacct                                                                                                                                                                                                                                                    >  2:164241‑M1/1‑244 (MQ=255)                                                             aaaaCGCCGTTGCCTGAAATGCGGCACGTCGATGGACAGCTACCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtgacggcgaagtggcaccaccgagagaagatccggtgccattacctgagc                                                                                                                                                                                                                                                <  2:798597‑M1/300‑61 (MQ=255)                                                                   tcgtTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgt                                                                                                                                                                                                                                          >  1:624613‑M1/1‑234 (MQ=255)                                                                        gCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGNATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaaaggngaagtggcaccaccgacagaagatccggtgccattacctgagctgccgtgcgaa                                                                                                                                                                                                                                     >  2:778012‑M1/1‑229 (MQ=255)                                                                             aaaTGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCACGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatc                                                                                                                                                                                                                                >  2:814149‑M1/1‑224 (MQ=255)                                                                               aTGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGCAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgcccttacctgagctgccgtgcgaaaaatcag                                                                                                                                                                                                                              >  2:401780‑M1/1‑222 (MQ=255)                                                                                         gCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgcgctgccgtgcgaaaaatcagatgcttattt                                                                                                                                                                                                                    >  1:825896‑M1/1‑212 (MQ=255)                                                                                         gCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttattt                                                                                                                                                                                                                    <  1:91151‑M1/300‑89 (MQ=255)                                                                                         gCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttattt                                                                                                                                                                                                                    >  2:380833‑M1/1‑212 (MQ=255)                                                                                              ggACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgc                                                                                                                                                                                                               >  1:616712‑M1/1‑207 (MQ=255)                                                                                                atagcTATCTCATCGAGCCGAAACGTGAGTTGCATGTCTGGGGTAATAACCCAACCTGCGACGGTTACGAGATCTAAGAGGGCGAATTCCGCATTAAAGTTTATGACTGCCCGATCGTTGAGTGTTAAAAATTTGGCTCGGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTTTAAAAAacattctacgtagcggcgaagtggcaccaccgagagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctg                                                                                                                                                                                                             <  2:164785‑M1/298‑96 (MQ=255)                                                                                                 cAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgc                                                                                                                                                                                                            >  1:829658‑M1/1‑204 (MQ=255)                                                                                                           aTCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgc                                                                                                                                                                                                  <  1:375415‑M1/300‑107 (MQ=255)                                                                                                               aTCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgcc                                                                                                                                                                                              <  1:473388‑M1/300‑111 (MQ=255)                                                                                                                tCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTNAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccg                                                                                                                                                                                             <  1:582267‑M1/300‑112 (MQ=255)                                                                                                                 ccGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttctgtctcttatacacatctcgcag                                                                                                                                                                                            <  1:646998‑M1/300‑113 (MQ=255)                                                                                                                             ttGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggc                                                                                                                                                                                <  1:456839‑M1/300‑125 (MQ=255)                                                                                                                             ttGCATGTCCGTGGTAATAACCCAACCTGCGACGGTTACTAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTTGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcttgacggtgctgccggtgtgttcctggc                                                                                                                                                                                <  1:456848‑M1/300‑125 (MQ=255)                                                                                                                                         ggTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAGTTCCGGATTAAAGGTTATGACTGCCCGGTCGTTGAGTGTGGAAAATGTGGCTCTGAAATGCACCTGCACATGGGGCGTGTCGGTAAATAAATGTCCTGCACCAAAGAAGAGTGTAAAA‑‑‑‑‑‑‑‑AAGATTTTACGTAACGGCGACGTGGTACCACAAAAAGAAGATCCGATGCCATTCCCTGATCTTCCGTGCAAAAACTCCGATGCTTGTTTCGTGCTGCATGACAGTTCCGCCGGTGCGTTCCTAACTGCCAACACttt                                                                                                                                                            >  1:86240/1‑300 (MQ=255)                                                                                                                                          gTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttc                                                                                                                                                                   >  2:784569‑M1/1‑163 (MQ=255)                                                                                                                                          gTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCCGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcatggctgccaacactttc                                                                                                                                                                   >  1:828240‑M1/1‑163 (MQ=255)                                                                                                                                               aaCCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaa                                                                                                                                                              >  1:758534‑M1/1‑158 (MQ=255)                                                                                                                                               aaCCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTATGAAATGCACCTGAAAATGGGGCGTTTAGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggccccaacaaaagaagatccggtgccattcaccgagctgccgtgagaaaaatcaaatgcttattccgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcacgaa                                                                                                                                                              >  2:404142‑M1/1‑158 (MQ=255)                                                                                                                                                            aCGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacg                                                                                                                                                 <  1:510666‑M1/300‑156 (MQ=255)                                                                                                                                                              ggTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcg                                                                                                                                               >  1:32553‑M1/1‑143 (MQ=255)                                                                                                                                                                     cgatCGACGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTAGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgcccacactttcccgaaatcgcgtgcaacgcgtgcgcca                                                                                                                                        >  2:120755‑M1/2‑136 (MQ=255)                                                                                                                                                                               gggCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaag                                                                                                                              <  1:483702‑M1/300‑175 (MQ=255)                                                                                                                                                                                         cGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcg                                                                                                                    <  1:397507‑M1/300‑185 (MQ=255)                                                                                                                                                                                                 aGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcg                                                                                                            >  1:726352‑M1/1‑108 (MQ=255)                                                                                                                                                                                                     tATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGTGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccg                                                                                                        <  1:122898‑M1/300‑197 (MQ=255)                                                                                                                                                                                                               cGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcntgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaa                                                                                              >  1:586870‑M1/1‑94 (MQ=255)                                                                                                                                                                                                                 aTCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttccagaaatcgcgtgaaacgcgtgcgccaatggtggaagagctttatcgcttccgcgaccgtatgcaggaaaa                                                                                            >  2:214494‑M1/1‑92 (MQ=255)                                                                                                                                                                                                                  tCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagacccggtgccattccctgagctgccgtgcgaaaactcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaagcgcgtgcgcaaatggtggaagagctttatcgcttccgcgaccgtctgccggaaaaa                                                                                           >  2:238700‑M1/1‑91 (MQ=255)                                                                                                                                                                                                                        aGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgt                                                                                     <  1:322143‑M1/300‑216 (MQ=255)                                                                                                                                                                                                                             gAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatct                                                                                <  1:443352‑M1/300‑221 (MQ=255)                                                                                                                                                                                                                                  atttggctCTGAAATGCACCGGAAAATGGTGCGTTTCGTTAAATACATGGCCTGCACCAACGAAGAGGGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtganggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgancgtctgccggaaaaactgcgttatctggccg                                                                           <  2:599978‑M1/297‑226 (MQ=255)                                                                                                                                                                                                                                  aTGTTGCTCTGAAATACACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccg                                                                           <  2:362287‑M1/300‑226 (MQ=255)                                                                                                                                                                                                                                      ggCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgc                                                                       <  1:170888‑M1/300‑230 (MQ=255)                                                                                                                                                                                                                                       gCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcg                                                                      >  1:82269‑M1/1‑70 (MQ=255)                                                                                                                                                                                                                                            gAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccaca                                                                 >  1:762903‑M1/1‑65 (MQ=255)                                                                                                                                                                                                                                              aaTGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagc                                                               <  1:756088‑M1/300‑238 (MQ=255)                                                                                                                                                                                                                                                         aaaTGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaa                                                    <  1:88596‑M1/300‑249 (MQ=255)                                                                                                                                                                                                                                                                  gTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataag                                           <  2:6647‑M1/300‑258 (MQ=255)                                                                                                                                                                                                                                                                  gTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataag                                           <  1:302163‑M1/300‑258 (MQ=255)                                                                                                                                                                                                                                                                    ttCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcttgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataagac                                         <  1:311002‑M1/300‑260 (MQ=255)                                                                                                                                                                                                                                                                    ttCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataagac                                         <  1:310541‑M1/300‑260 (MQ=255)                                                                                                                                                                                                                                                                    ttCGGGAAATACATGGCCTGCACCAACGAAGAGCGTAAAAAagattttgcttaacggcgaagttgcaccaccgagagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcttgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtgtaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataagac                                         <  2:293845‑M1/300‑260 (MQ=255)                                                                                                                                                                                                                                                                       ggTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataagaccat                                      >  2:751584‑M1/1‑38 (MQ=255)                                                                                                                                                                                                                                                                       ggTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataagaccat                                      >  1:784417‑M1/1‑38 (MQ=255)                                                                                                                                                                                                                                                                               cATGACCTGCACCAACGAAGAGTGTAAAAAagattctacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataagaccatggttcgct                              <  2:466290‑M1/300‑271 (MQ=255)                                                                                                                                                                                                                                                                                 tGGCATGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataagaccatggttcgcttt                            <  2:700159‑M1/300‑273 (MQ=255)                                                                                                                                                                                                                                                                                  ggCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatgtggccgatgcgccacagcaggatccggaaggtaataagaccatggttcgcttta                           >  2:783907‑M1/1‑27 (MQ=255)                                                                                                                                                                                                                                                                                  ggCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataagaccatggttcgcttta                           >  1:817746‑M1/1‑27 (MQ=255)                                                                                                                                                                                                                                                                                  ggCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgcaggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataagaccatggttcgcttta                           >  1:654343‑M1/1‑27 (MQ=255)                                                                                                                                                                                                                                                                                   gCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataagaccatggttcgctttag                          >  1:521493‑M1/1‑26 (MQ=255)                                                                                                                                                                                                                                                                                   gCCTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggnggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataagaccatggttcgctttag                          >  2:726274‑M1/1‑26 (MQ=255)                                                                                                                                                                                                                                                                                     cTGCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataagaccatggttcgctttagcc                        <  1:70199‑M1/300‑277 (MQ=255)                                                                                                                                                                                                                                                                                       gCACCAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataagaccatggttcgctttagccgt                      >  1:82277‑M1/1‑22 (MQ=255)                                                                                                                                                                                                                                                                                          ccAACGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgaaggtgctgccggtgtgttcatggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttacgcggccgtctgcaggaaaaactgcgttatctggccgatgcgccacagcaggatcaggaaggtaataagaccatggttcgctttagccgtaca                   >  2:91970‑M1/1‑19 (MQ=255)                                                                                                                                                                                                                                                                                            aaCGAAGAGTGTAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataagaccatggttcgctttagccgtaaaac                 >  1:78030‑M1/1‑17 (MQ=255)                                                                                                                                                                                                                                                                                                  gagTGTAAAAAagattttacgtaacggcgaagtggcaccaacgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatacttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttccagaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacggcaggatccggaaggtaataagaccatggttcgctttagccgtaaaaccaaaca           >  2:784999‑M1/1‑11 (MQ=255)                                                                                                                                                                                                                                                                                                    gtgtAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataagaccatggttcgctttagccgtaaaaccaaacagc         >  1:775183‑M1/1‑9 (MQ=255)                                                                                                                                                                                                                                                                                                    gtgtAAAAAagattttacgtaacggcgaagtggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaacgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataagaccatggttcgctttagccgtaaaaccaaacagc         >  1:32409‑M1/1‑9 (MQ=255)                                                                                                                                                                                                                                                                                                           aaagattttacgtaacggcgaattggcaccaccgaaagaagatccggtgccattacctgagctgccgtgcgaaaaatcagatgcttatttcgtgctgcgtgacggtgctgccggtgtgttcctggctgccaacactttcccgaaatcgcgtgaaacgcgtgcgccactggtggaagagctttatcgcttccgcgaccgtctgccggaaaaactgcgttatctggccgatgcgccacagcaggatccggaaggtaataagaccatggttcgctttagccgtaaaaccaaacagcaatatgt  <  2:788677‑M1/300‑299 (MQ=255)                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                           GGAAAACGAAGTGCTGAACGTGCTGGAAGGCGAAGATGCTGAAACCAACGCGCTGCGCGCAAAACGTCGTTGCCCGAAATGCGGCACGGCGATGGACAGCTATCTCATCGATCCGAAACGTAAGTTGCATGTCTGTGGTAATAACCCAACCTGCGACGGTTACGAGATCGAAGAGGGCGAATTCCGCATTAAAGGTTATGACGGCCCGATCGTTGAGTGTGAAAAATGTGGCTCTGAAATGCACCTGAAAATGGGGCGTTTCGGTAAATACATGGCCTGCACCAACGAAGAGTGTAAAAACACACGTAAGATTTTACGTAACGGCGAAGTGGCACCACCGAAAGAAGATCCGGTGCCATTACCTGAGCTGCCGTGCGAAAAATCAGATGCTTATTTCGTGCTGCGTGACGGTGCTGCCGGTGTGTTCCTGGCTGCCAACACTTTCCCGAAATCGCGTGAAACGCGTGCGCCACTGGTGGAAGAGCTTTATCGCTTCCGCGACCGTCTGCCGGAAAAACTGCGTTATCTGGCCGATGCGCCACAGCAGGATCCGGAAGGTAATAAGACCATGGTTCGCTTTAGCCGTAAAACCAAACAG  >  NC_000913/1332961‑1333558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.