Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,396,772	T→C	F236F (TTT→TTC)	amiB →	N‑acetylmuramoyl‑L‑alanine amidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,396,772	0	T	C	100.0%	216.8 / NA	58	F236F (TTT→TTC)	amiB	N‑acetylmuramoyl‑L‑alanine amidase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/33);  total (25/33)

GGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGATTTACAGTT  >  NC_000913/4396478‑4397065                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        ggTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAgg                                                                                                                                                                                                                                                                                                    <  1:324113/300‑1 (MQ=255)       gAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGtttt                                                                                                                                                                                                                                                                                              >  1:701566/1‑300 (MQ=255)                     cGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACgggg                                                                                                                                                                                                                                                                                <  2:234412/300‑1 (MQ=255)                           cGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACt                                                                                                                                                                                                                                                                          <  2:453922/300‑1 (MQ=255)                             gTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGNGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACttt                                                                                                                                                                                                                                                                        <  1:529592/300‑1 (MQ=255)                                gTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTAtc                                                                                                                                                                                                                                                                     <  2:658162/300‑1 (MQ=255)                                 tCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTAtct                                                                                                                                                                                                                                                                    <  1:155534/300‑1 (MQ=255)                                             tCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAACTTATCATCGCTATTGCTGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑Ggc                                                                                                                                                                                                                                                      >  2:349758/1‑300 (MQ=255)                                                  gAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGt                                                                                                                                                                                                                                                 <  1:614368/300‑1 (MQ=255)                                                    aCCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGa                                                                                                                                                                                                                                               <  1:711682/300‑1 (MQ=255)                                                      cGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGAtg                                                                                                                                                                                                                                             <  1:344654/300‑1 (MQ=255)                                                       ggCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGAtgt                                                                                                                                                                                                                                            <  1:449692/300‑1 (MQ=255)                                                         cgcgcAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTgg                                                                                                                                                                                                                                          <  1:359763/300‑1 (MQ=255)                                                         cgcgcAATCCGTGTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTANGACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGTTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGTGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTgg                                                                                                                                                                                                                                          <  2:540955/300‑1 (MQ=255)                                                            gcAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGTTGATGG‑‑GGCGCAGTGATGTGGCAc                                                                                                                                                                                                                                       <  1:389502/300‑1 (MQ=255)                                                                   gTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCa                                                                                                                                                                                                                                >  2:436644/1‑300 (MQ=255)                                                                    tttAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCaa                                                                                                                                                                                                                               <  1:198223/300‑1 (MQ=255)                                                                         aaCGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGATGATCCGATGTTCAAAGGCGTTTTAACCCGTGAAGGGGACTACTTTATCTCGGTGGTGG‑‑GGTGCAGTGATGTGGAACGTAAGTaaaaaga                                                                                                                                                                                                                          >  2:258077/1‑297 (MQ=255)                                                                                    aCCGCATTATGGTTGTTATCAGCAGTAATACGGTAACACGTCCGGCAGAGCGCGAGAATGCTAACACTTTCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTAAGGACTCTGGCGCTATCTGCCCCGGGGGTACGCGGGAGAAAAATGCCACCACCGCCATCGCGCGNAACTTGCGTACTTTGCTCAGTGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGANGTGGCACGTAAGCAAAACGCCAATTTCCTCg                                                                                                                                                                                                               <  2:513946/300‑1 (MQ=255)                                                                                        cACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGAAAGTAGGCAAAACGCCAATTTCCTCGTGTc                                                                                                                                                                                                           >  2:401081/1‑300 (MQ=255)                                                                                               ggTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGGTGG‑‑GGAGCAGTGGTGTGGCACGTAAGCAAAACGCAAATTTCATCGTGTCGATTccc                                                                                                                                                                                                    >  2:271319/1‑298 (MQ=255)                                                                                                       cagcagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCNGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGc                                                                                                                                                                                            <  1:491814/300‑1 (MQ=255)                                                                                                          cagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGGTGG‑‑GGAGCAGTGGTGTGGAAAGTAAGAAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGc                                                                                                                                                                                         >  2:296376/1‑300 (MQ=255)                                                                                                                  cGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGGTGG‑‑GGTGCAGTGATGTGGCANGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCANCGaaac                                                                                                                                                                                 >  2:601745/1‑298 (MQ=255)                                                                                                                                              ggCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGt                                                                                                                                                     >  1:303357/1‑300 (MQ=255)                                                                                                                                              ggCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTAGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGACAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGt                                                                                                                                                     >  2:664345/1‑300 (MQ=255)                                                                                                                                                  aaCACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGctc                                                                                                                                                 <  1:447495/300‑1 (MQ=255)                                                                                                                                                        gtcGATAGAATCATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCATGGANATAGCGGCCCCGGTGGGACGCGGGAGAAAACTGTCACCATCGCCATCGCGCGGAAATTGCGTACTTTGCTCACTGACGATCCGATGTTCAAAGGCGTTTGAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACNCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAAc                                                                                                                                           <  2:671626/298‑1 (MQ=255)                                                                                                                                                         gCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGTTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAANTTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAAcc                                                                                                                                          <  1:522465/300‑1 (MQ=255)                                                                                                                                                          cggtAAAATTATCATCGCTATTGATGCAGGACACGGCGGTCAGGACCCTGGCGCTAGCGGCCCCGGTGGGACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGGAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCg                                                                                                                                         <  2:560310/297‑1 (MQ=255)                                                                                                                                                          caatagatgtactcgctcTATTGATGCCGGACACTGCGGTCAGTACCCTGGCACTAGCTGCCCCGGTGGTACGCTGTAGAAAAACGTCACCATCGCCATCGTGCGTAAATTGCTTACTTTGCTCAATTACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGTTGATGG‑‑GGCGCAGTGAGGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTaatcg                                                                                                                                         <  2:385815/283‑4 (MQ=255)                                                                                                                                                          cGATAGAATTATCACCGCTATTGATGCAGAGCGCGGCGGTCAGGATTCGGGCGCTGTAGGCCCCGTTGGTACGCGGGAGAAAAAGGTAACAATCACCATCGCGCGTAAACTACCTACTTTGCTCAATGACGATCCGAGGTTCAAAGGTGTTTTAACCCGTGACGGGGATTGCTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGGTTCACGCTGAGGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGTTCTCTAACCg                                                                                                                                         <  2:24635/300‑1 (MQ=255)                                                                                                                                                          cGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCg                                                                                                                                         <  1:441124/300‑1 (MQ=255)                                                                                                                                                              aaaaTTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTAGGTGATGG‑‑GGCGAAGTTGTGTGGGAAGTAGGAAAAACGCCAATTTCCTAGTGTCGAGTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGATTCCGTCTGGGTGCTTTCTAACCATcgc                                                                                                                                     >  2:350003/1‑300 (MQ=255)                                                                                                                                                                           cTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCAAGTAAGAAAAAGGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTATAACCGTCGCGCCAACCGTGaaa                                                                                                                        >  2:152628/1‑300 (MQ=255)                                                                                                                                                                                             gcggGCAGGACCCTGTCGCTAGCTGCCCCGGTGGTATGCAGGAGAAAACGGCCACCAGCGCCATCGCGCGTAAATTACGTACTTCGCGCAACTACGATCCGATATTCAAAGGCNTTTTAACCCGTGACGGGGACTACTTTATCTCGGTGATGG‑‑GGCGCAGTAATGTGGCACGTAANCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGagc                                                                                                      <  2:585056/300‑1 (MQ=255)                                                                                                                                                                                               ggTCAGGACCCTGGCGCTATCGGCCCCGGTGNTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGNCGGGGATTACTTTATCTCGGTGGTGG‑‑GGCGCAGTGGGGTGGCAAGTAAGCAAAAAGCCAATTTACTAGTGTCGATTAACGCTGATGCCGCACCGAACAGCAGTGTGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAGTGGCCAGCTGGCTGGagcag                                                                                                    >  2:514727/1‑300 (MQ=255)                                                                                                                                                                                               ggTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGagcag                                                                                                    >  1:734325/1‑300 (MQ=255)                                                                                                                                                                                                      aCCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAAAGCCAATTTCCTAGTGTAGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCAAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACgaaa                                                                                             >  2:397966/1‑298 (MQ=255)                                                                                                                                                                                                       cccTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGaa                                                                                            >  1:35460/1‑300 (MQ=255)                                                                                                                                                                                                               cTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGGTGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCgg                                                                                    >  2:694514/1‑300 (MQ=255)                                                                                                                                                                                                               cTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCgg                                                                                    >  1:623737/1‑300 (MQ=255)                                                                                                                                                                                                                 aTCGGCCCCGGTGGTACGCGGGAGAAAAATGNCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGGTTACTTTATCTNGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCCGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCAAGAGAAACAGTCGGAg                                                                                  >  2:522907/1‑300 (MQ=255)                                                                                                                                                                                                                     gCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTNGTGG‑‑GGCGCAGTGGTGTGGAAAGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGNTGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAgctgc                                                                              >  2:586480/1‑300 (MQ=255)                                                                                                                                                                                                                          tttattACGCTGGAGAAAAATGTAACCACCGCCGTCGTGCGTAAATTGCGTACTCTACTCAACTACACTCCGATATTCAAAGCCGGTGGAACCCGGGACGGGGATTAATTTATCTCGGTGATGG‑‑GGCGCAGTGATGGGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCACTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTTAAATGGCCAGCTGGCTGGAGCAGCACGAGAAATAGCCTGAGCTGCtgggt                                                                         <  2:35111/295‑1 (MQ=255)                                                                                                                                                                                                                           gtggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtgggtg                                                                        >  1:298579/1‑300 (MQ=255)                                                                                                                                                                                                                            tggtACGCGGGAGAAAAATTTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCATTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAATCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtgggtgg                                                                       <  2:302226/300‑1 (MQ=255)                                                                                                                                                                                                                            tggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtgggtgg                                                                       <  1:258255/300‑1 (MQ=255)                                                                                                                                                                                                                                   cgGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGt                                                                >  1:17619/1‑300 (MQ=255)                                                                                                                                                                                                                                    gggAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTg                                                               <  1:382998/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          cGTACTTTGCTCAATGACGATCCAATGTTCAAAGACGTTTTAACCCGTGACGGGNATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGNTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGc                         <  2:686980/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                ttGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGGTGGAAGGCCCCTCCA‑‑CGGCACGTAGGGAGAACGCCAATTTCCTAGTGGCGATTCACGCTGATGCAGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTGCTCTCTAAACGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGAAGTAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGGGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCtccctacaccaagcg                   >  2:313043/1‑286 (MQ=255)                                                                                                                                                                                                                                                                                ttGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCg                   >  1:574160/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    tCAATGNCGATCCGACGGTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTANCTCGGGGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACCGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGc               <  2:605039/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         gACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGAt          <  1:356958/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         gACGAGCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCANGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCNCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGAt          <  2:566827/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               ccGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGATTTACAg    <  2:706286/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 gATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGGTGG‑‑GGGGCCGTGCTGTGGAAAGTAGGCAGAACGCCAATTTCCTAGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGATGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCNGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAGCAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGATTTACAGtt  >  2:630185/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        GGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGG‑‑GGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGATTTACAGTT  >  NC_000913/4396478‑4397065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑