Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,857	T→C	C388R (TGC→CGC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,857	0	T	C	97.4%	259.2 / NA	77	C388R (TGC→CGC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (30/45);  total (32/45)
Fisher's exact test for biased strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑ACG‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCC  >  NC_000913/4637567‑4638152                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                           cTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGNAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCACGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCNTGGGAATTTACTGGATAACGCCGTTGATGTTACCACCGAGAGCGGTCGCATAACGc                                                                                                                                                                                                                                                                                                   <  2:795313/300‑1 (MQ=255) cTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGc                                                                                                                                                                                                                                                                                                   <  1:1604/300‑1 (MQ=255) cTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATGGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGANTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGTTGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGc                                                                                                                                                                                                                                                                                                   <  2:752261/300‑1 (MQ=255)    gTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTaa                                                                                                                                                                                                                                                                                                >  1:473982/1‑300 (MQ=255)      tttACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAgc                                                                                                                                                                                                                                                                                              <  1:11305/300‑1 (MQ=255)      tttACCGACAACACGCTGACGAAAACTGCGCGTATGCATGCAGTGGTAGAAACGTTACTACGCCAGGCAAGACGGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCTGCATTATTTCGCCGCGTCAGCTAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGATGTTAATGTTGCTGCTGAACCGGCGCTACTGTAGCATGCCCTTGTGAATCTACTCTATCACACCACTGATTTGACCACCGAGAGCGGTCGCATAACGCTAAgc                                                                                                                                                                                                                                                                                              <  2:387717/300‑1 (MQ=255)                acaTTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTAAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTAATGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTAACCCCGAGAGCGGTCGCATCAAGCTAAGTGCCGAAGTgg                                                                                                                                                                                                                                                                                    >  2:745406/1‑300 (MQ=255)                           cAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTCTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAacac                                                                                                                                                                                                                                                                         <  2:775516/300‑1 (MQ=255)                             aaacgcgcGTATGCAGGCACGGGTAGAAACGTTACTACGCCAGGCAAGATTGGAGAATCTGCAGGGAGTCGTTCTGGCTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCTTCGTTACTGGAGCAGGCGCTTTTGAATTTACTGGCTAATACTAGCAATGCTACCACCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGt                                                                                                                                                                                                                                                                       <  2:161437/296‑1 (MQ=255)                             aaaTGCGCGTATGCAGGCATTGTTATAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGGCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATCTACTGGATAACAACAGCGAGTTGACCACCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGt                                                                                                                                                                                                                                                                       <  2:161788/300‑1 (MQ=255)                              aatacacggaTGCAGGCATTGGGAGAAACGTTATTACGCCAGGAGAGACTTGAGAATCGTTAGGAAGTCATTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCACGTCGGCTAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTGCATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCTGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACACCAGCGATTCGACCACCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTc                                                                                                                                                                                                                                                                      <  2:29279/291‑1 (MQ=255)                                        tGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAgg                                                                                                                                                                                                                                                          <  2:851031/300‑1 (MQ=255)                                        tGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAgg                                                                                                                                                                                                                                                          <  1:20288/300‑1 (MQ=255)                                                  ggTAGAAACGCTACTATGCCAGGCAAGACTGGAGAATCGTCAGTAAGTCGTGCTGACTGTTGTTGATGTGTCGACATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTTAGGTTAATGTTGCTGCTGAACCCGCGTTACTGGAGCAGGCGCTGGGTAATCTACTTGATAACACCATCGATTCTACCACCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATAc                                                                                                                                                                                                                                                <  2:2274/300‑1 (MQ=255)                                                     aGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCgg                                                                                                                                                                                                                                             <  1:174482/300‑1 (MQ=255)                                                       aaaCGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTNGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTa                                                                                                                                                                                                                                           >  1:628520/1‑300 (MQ=255)                                                           gTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTgg                                                                                                                                                                                                                                       <  1:358433/300‑1 (MQ=255)                                                            ttcctacGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTCTTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCAGAGAGCGGTCGCACAACGCCCCGCACAGAAGTGGATCAGGAATACGTC‑GCT‑CTTCATGTGCTGGCTGCCGGTAGTGGt                                                                                                                                                                                                                                      >  2:451213/4‑300 (MQ=255)                                                            ttACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGTCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCATGAACACGTC‑GCC‑CTTAAGGGGCTGGATACCGGTAGTGGt                                                                                                                                                                                                                                      <  1:679258/300‑1 (MQ=255)                                                            ttACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAACAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGACCCGGCGTTACTGGAGCCGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATACCGCTCCGCACCGAAGTGGATCAGGAACACGTC‑GCC‑CTCAAGGTGCTGGCTACCGGTAGTGGt                                                                                                                                                                                                                                      >  2:493790/1‑300 (MQ=255)                                                            ttACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGTGGTCGCATCACGCTACGCGCCGAAGTGGATCAGGAACCCGCC‑GCC‑CTTAAGGTGCTGGCTACCGGTAGTGGt                                                                                                                                                                                                                                      >  2:680760/1‑300 (MQ=255)                                                                 acGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTcc                                                                                                                                                                                                                                 <  1:280206/300‑1 (MQ=255)                                                                  cGCCATGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCt                                                                                                                                                                                                                                >  1:689583/1‑300 (MQ=255)                                                                   gccattcacgACTGGAGAATCGTCGGGAATTCGTTCTGACTGTTGTTGATGTGTCGGCATTATTTCGCCGCGTCATCAAAGCGCGCACCGTGCAGTGGGCAGAAAAAAACATCACTCTACATTTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCTTCGTTACTTTACCATGCGCTTTGGAATTTACTTGATAACGCCATTGATTTTACCACCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTg                                                                                                                                                                                                                               <  2:69053/291‑1 (MQ=255)                                                                   gCCAGGCAAGACTTGAGAAGCGTCGGGAAGTCGTTCTGACTGGTGTGGATTTGGCGGCATTGTTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACGTAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTTGGGAATTTACTGGATAACGCCATTGATTTGACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTg                                                                                                                                                                                                                               <  2:187641/300‑1 (MQ=255)                                                                   gCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTg                                                                                                                                                                                                                               <  1:541689/300‑1 (MQ=255)                                                                   gCCAGGCAAGACGGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTg                                                                                                                                                                                                                               <  2:480392/300‑1 (MQ=255)                                                                      aGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACt                                                                                                                                                                                                                            >  1:879384/1‑300 (MQ=255)                                                                            gACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACgcgc                                                                                                                                                                                                                      <  2:490976/300‑1 (MQ=255)                                                                                              aaGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTAGGGAATTTACTGGATAACGCCATCGATTTTACCCCAGAGAGCGGTCGCATAACGCTAAGCGCAGAAGTGGATCAGGAACACGTC‑GCA‑CCTAAGGTTCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGacc                                                                                                                                                                                                    >  1:354425/1‑298 (MQ=255)                                                                                                        gACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTActc                                                                                                                                                                                          <  1:650719/300‑1 (MQ=255)                                                                                                            gttgTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAANGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTg                                                                                                                                                                                      >  1:587069/3‑300 (MQ=255)                                                                                                            gttgTTGATGTGGCGACATTATTTCGCCGCGTCAGCGAAGCGTGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCAATGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATAAAGCTAAGCGCCGGAGTGGATCGGGAGCAAGT‑‑AGGAGTTAAGGTGCTGGATACTTTTAGTGGTATTCCTGACTACGCGCTGTCATGTACTTTCGAACACTTTTACTCTTTg                                                                                                                                                                                      >  2:434097/3‑300 (MQ=255)                                                                                                             ttgTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGc                                                                                                                                                                                     >  1:190633/2‑300 (MQ=255)                                                                                                             ttgTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCAATCTAAATGTTATGCCCAATGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGCGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCAGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTCCTCTTTGc                                                                                                                                                                                     >  1:335462/2‑300 (MQ=255)                                                                                                               gTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGccg                                                                                                                                                                                   >  1:797177/1‑299 (MQ=255)                                                                                                                                  ttcgccgcGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACACCACTCTATGTTTTATTCCTACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATANCGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCaaaaaa                                                                                                                                                               <  2:217271/298‑1 (MQ=255)                                                                                                                                                gAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTgggg                                                                                                                                                 <  2:563374/300‑1 (MQ=255)                                                                                                                                                gAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTgggg                                                                                                                                                 <  1:842248/300‑1 (MQ=255)                                                                                                                                                 aaGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACCTGTTATGCCCACTTATGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGTGGAATCTACTGGATAACGCCATCGATTTTANCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGt                                                                                                                                                <  2:219491/300‑1 (MQ=255)                                                                                                                                                 aaGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTCTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGt                                                                                                                                                <  2:780058/300‑1 (MQ=255)                                                                                                                                                  aGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGtt                                                                                                                                               >  1:562728/1‑300 (MQ=255)                                                                                                                                                      cgcACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCg                                                                                                                                           <  1:433720/300‑1 (MQ=255)                                                                                                                                                      cgcACCGTGCAGTGGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCATGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCNAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCg                                                                                                                                           <  2:200296/300‑1 (MQ=255)                                                                                                                                                                   tGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAgg                                                                                                                              <  2:551341/300‑1 (MQ=255)                                                                                                                                                                                     ctctACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGGTAAGCGCCGAAGTGGATAAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAACGGTATTCCTGACTACGCGCTGCCACGTATTTTTGAACGCTCTTACTCTTTGCCGCGTGCCCCT‑GGGCACAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGCGAGGTCGCCCGTTTGTTTaccg                                                                                                            >  2:849080/1‑297 (MQ=255)                                                                                                                                                                                     ctctACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACg                                                                                                            >  1:128627/1‑300 (MQ=255)                                                                                                                                                                                     ctctACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAAAGCTAAGCGCCGAAGTGGATCAAGAAAACGTCGAC‑‑CTTAAGGTGCTGGATACAGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCACCT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCCGTGAGGTCGCCCGTTTGTTTaccg                                                                                                            >  2:542849/1‑297 (MQ=255)                                                                                                                                                                                     ctctACATGTTATGCCCACTGAGGTTAATGTNGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGACTTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGTATAACGCTAAGNGCGGAAGTGGATAAGGAATGAGTG‑GGC‑CTTAAGGTGTTGGGTACGGATAGCGGTATTCTTGAGTAAGAGCTGTCAAGTATTTTTGACCGATCGTACTCTTTGCCGCGTGCACCT‑GGGCCAAACGGAACCGGTCTGGGATTGGCGTTcttccacacggtcgcccttttatttacct                                                                                                            >  2:623558/1‑272 (MQ=255)                                                                                                                                                                                       ctacATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCA‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGc                                                                                                          >  1:420828/4‑300 (MQ=255)                                                                                                                                                                                       ctacATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATCTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACAAGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTAAGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGc                                                                                                          >  1:421814/4‑300 (MQ=255)                                                                                                                                                                                             gTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTNGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTCAGCGCCGAAATGGATAAGGAACAAGTC‑GAA‑CTTAAGGTTTTGGGTAGTGGTGGTGGTCTTCCTGCCTACGCGCTGTAACGTCTTTTTGAACGCTTTTACTATTTGTAGCGTGCAGATAGGACAACAAA‑CACNGATCTGAGGTTGACATTCGTCAATGCGGTCGCCAGTTTGTTTACCGCCGAAGTc                                                                                                    >  2:799147/1‑300 (MQ=255)                                                                                                                                                                                             gTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTc                                                                                                    >  1:219502/1‑300 (MQ=255)                                                                                                                                                                                                 tgCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGc                                                                                                <  1:801059/299‑1 (MQ=255)                                                                                                                                                                                                      actgAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTgcgc                                                                                           <  1:452063/297‑1 (MQ=255)                                                                                                                                                                                                         gcggTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAAc                                                                                        >  1:780451/3‑300 (MQ=255)                                                                                                                                                                                                         gAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAAc                                                                                        >  1:836018/1‑300 (MQ=255)                                                                                                                                                                                                           ggttaatattGCTGCTGAACCTGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGt                                                                                      <  2:22009/292‑1 (MQ=255)                                                                                                                                                                                                           ggTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGt                                                                                      >  1:738951/1‑300 (MQ=255)                                                                                                                                                                                                            gTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATTAGGAACACGTC‑GCA‑CTTAAGGCGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAC‑GGGCAAAAAAGCAGCGGTATGTGGTTGTCGCTCGTCAGTGAGATCGACCGTCCATCTCACGGCGAAATCCCGCTGCGCAACGTg                                                                                     >  2:311069/1‑300 (MQ=255)                                                                                                                                                                                                                  gTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGaa                                                                               <  1:92940/300‑1 (MQ=255)                                                                                                                                                                                                                     gctgctGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGt                                                                            <  2:484171/300‑1 (MQ=255)                                                                                                                                                                                                                     gctgctGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGt                                                                            <  1:543463/300‑1 (MQ=255)                                                                                                                                                                                                                       tgctgAACCTGCGTTACTGGAGCAGGCGCTGTGGAATTTACTGGATAGCGCCAGCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTgg                                                                          <  2:446137/300‑1 (MQ=255)                                                                                                                                                                                                                        gctgAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGc                                                                         >  1:337455/1‑300 (MQ=255)                                                                                                                                                                                                                              ccGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTg                                                                   <  1:259754/300‑1 (MQ=255)                                                                                                                                                                                                                                 gCGTTACTGGAGCATGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGcc                                                                <  2:546064/300‑1 (MQ=255)                                                                                                                                                                                                                                  cggtACTGGAGCAGGCGCTGGTGAATTTACTGGATAACGCCATCGATTTTACCCCCTAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCNCTGTCACGTATTTTTGAACGCTTTTANTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCt                                                               <  2:584603/297‑1 (MQ=255)                                                                                                                                                                                                                                                        gaaTTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACtt                                         >  1:219922/2‑300 (MQ=255)                                                                                                                                                                                                                                                           tttACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTcac                                      <  1:31543/300‑1 (MQ=255)                                                                                                                                                                                                                                                             tACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACAt                                    <  1:59034/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 ggATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCt                                >  1:703709/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       cGCCATCGATTCTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCATGAACACGTC‑GCC‑CTTAAGGTGCTAGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAAt                          <  2:141893/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         ccATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAAttc                        >  1:564556/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               cGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATcc  <  2:101507/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               cGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTTAAGGTGCNGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTACTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCNACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATcc  >  2:807064/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               cGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑GCC‑CTGAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTGTCACGTATTTTTGAACGCTTTTCCTCTTTGCCGCGTGCAAAT‑GGGCAAAAAAGCAGCNGTCTGGGGTTGGCGTTCGTCAGTGAGNTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGCCTTCGTATcc  <  2:571566/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                           CTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTC‑ACG‑CTTAAGGTGCTGGATACCGGTAGTGGTATTCCTGACTACGCGCTTTCACGTATTTTTGAACGCTTTTACTCTTTGCCTCGTGCAAAT‑GGGCAAAAAAGCAGCGGTCTGGGGTTGGCGTTCGTCAGTGAGGTCGCCCGTTTGTTTAACGGCGAAGTCACGCTGCGCAACGTGCAGGAAGGTGGCGTGCTGGCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCC  >  NC_000913/4637567‑4638152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑