Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,630,960	T→C	D76D (GAT→GAC)	slt →	soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,960	0	T	C	97.9%	323.8 / NA	96	D76D (GAT→GAC)	slt	soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (49/45);  minor base A (2/0);  total (51/45)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCG  >  NC_000913/4630667‑4631253                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                      ggTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCtgatg                                                                                                                                                                                                                                                                                                 <  1:43433/300‑1 (MQ=255)   ttAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGaa                                                                                                                                                                                                                                                                                               <  1:518508/300‑1 (MQ=255)     aGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATc                                                                                                                                                                                                                                                                                             <  2:132797/300‑1 (MQ=255)                      cgGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTc                                                                                                                                                                                                                                                                            >  1:536945/1‑300 (MQ=255)                        gCATGACGCTGCATCGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTAACTGGAATACCGTCAGATCACCGATGACCTGCTGAATCAACAGGCGGTGACTGTCAc                                                                                                                                                                                                                                                                          >  2:401575/1‑300 (MQ=255)                         cATGACGCTGCAGTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACt                                                                                                                                                                                                                                                                         <  2:454701/300‑1 (MQ=255)                          aTGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTa                                                                                                                                                                                                                                                                        <  1:82480/300‑1 (MQ=255)                            gACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCNCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATANTCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAAc                                                                                                                                                                                                                                                                      <  2:692966/300‑1 (MQ=255)                             aCGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTNGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAAACGGCGGTGACTGTCACTAACt                                                                                                                                                                                                                                                                     >  1:645984/1‑300 (MQ=255)                             aCGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACt                                                                                                                                                                                                                                                                     >  2:544150/1‑300 (MQ=255)                             aCGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACt                                                                                                                                                                                                                                                                     >  1:469148/1‑300 (MQ=255)                              cGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACtt                                                                                                                                                                                                                                                                    <  1:511288/300‑1 (MQ=255)                                  gcatttaggTATTTACACTTAGAGGATGCGCGTGTGGAAAAAGCCAAACAAGTTACCTGTCGTCTGTTGGCTGCCTGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGAGGAGCAGCGTAGCCGTTACGCGCAAATCAAGCATGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGAGTATCCACTTTATCCCTACCTGGAATACCGTCAGAGCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGtt                                                                                                                                                                                                                                                                <  2:434435/292‑1 (MQ=255)                                  gCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGtt                                                                                                                                                                                                                                                                >  1:529600/1‑300 (MQ=255)                                          gTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTac                                                                                                                                                                                                                                                        >  2:837911/1‑299 (MQ=255)                                            aTTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAAcc                                                                                                                                                                                                                                                      <  1:87593/300‑1 (MQ=255)                                            aTTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCAATAACTTTGTTCGCGCTAAcc                                                                                                                                                                                                                                                      >  1:654058/1‑300 (MQ=255)                                             tttACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAAccc                                                                                                                                                                                                                                                     <  1:223124/300‑1 (MQ=255)                                             gggacatgtAGAGGATGCGCTTGGGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTTGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTTGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAAccc                                                                                                                                                                                                                                                     <  1:411937/292‑1 (MQ=255)                                                 cacTTAGAGGATGCGCTTGTGGAAAAAGCCANACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCNTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCAATAACTTTGTTCGCGCTAACCCCACg                                                                                                                                                                                                                                                 >  2:629828/1‑300 (MQ=255)                                                 cacTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTAACTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACg                                                                                                                                                                                                                                                 >  2:426840/1‑300 (MQ=255)                                                  acTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGANGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGc                                                                                                                                                                                                                                                <  1:591636/300‑1 (MQ=255)                                                   cTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCt                                                                                                                                                                                                                                               >  1:565174/1‑300 (MQ=255)                                                   cTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAAAAATGGATGTGGTCGAACAAATGATGCATGGACTGAAAGATTATCCACTTTATCACTACATGGAATACCGTCAGACCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCt                                                                                                                                                                                                                                               >  2:79054/1‑300 (MQ=255)                                                   cTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTAAGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGNGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCt                                                                                                                                                                                                                                               >  2:216082/1‑300 (MQ=255)                                                         ggATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTtcctcc                                                                                                                                                                                                                                         >  2:328551/1‑300 (MQ=255)                                                              cgcTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTc                                                                                                                                                                                                                                    <  1:736056/300‑1 (MQ=255)                                                                cTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGc                                                                                                                                                                                                                                  >  1:413073/1‑300 (MQ=255)                                                                  tgtgGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCAc                                                                                                                                                                                                                                <  1:826051/300‑1 (MQ=255)                                                                          aaGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAAt                                                                                                                                                                                                                        <  1:517943/300‑1 (MQ=255)                                                                            gCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGGGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGGAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAAtct                                                                                                                                                                                                                      <  2:187920/300‑1 (MQ=255)                                                                                     gTTACCTGGCGGCTGTTGGCTGCCGGTGTCCGTCTACTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAACAGGCCTGGGATAATTGACAAATGGATGTGGCCGAACAAATGATGCCTGGACTGAAAGGTTANCCACTTTATCCCTACCTGGAATACCGTCAGCTCACCGATGACCTGCTGAATCAACCGGCGGTGACTGTCACTAACTTTGCTCGCGCTGACCCCACGCTTATTCCCGCTCGCACGCTGCAAtccagtcccgtc                                                                                                                                                                                                             >  2:204553/1‑290 (MQ=255)                                                                                         ccTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAGTGATGCATGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATg                                                                                                                                                                                                         >  2:230217/1‑300 (MQ=255)                                                                                         ccTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATg                                                                                                                                                                                                         >  2:230357/1‑300 (MQ=255)                                                                                          cTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGa                                                                                                                                                                                                        <  1:285191/300‑1 (MQ=255)                                                                                                gcTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGgc                                                                                                                                                                                                  >  1:392234/1‑300 (MQ=255)                                                                                                gcTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTAACTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGgc                                                                                                                                                                                                  >  2:30040/1‑300 (MQ=255)                                                                                                    ttGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATNGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATNCCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCgcgg                                                                                                                                                                                              <  2:702819/300‑1 (MQ=255)                                                                                                              gtgtCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACt                                                                                                                                                                                    >  2:661715/1‑300 (MQ=255)                                                                                                                   tgtctgCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGt                                                                                                                                                                               <  1:1618/300‑1 (MQ=255)                                                                                                                    gtctgCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTg                                                                                                                                                                              >  1:834244/1‑300 (MQ=255)                                                                                                                    gtctgCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTg                                                                                                                                                                              >  2:479583/1‑300 (MQ=255)                                                                                                                           gACGTTCAGCAGCGTGGCGCGAGCTGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGGATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGtt                                                                                                                                                                       <  2:14898/300‑1 (MQ=255)                                                                                                                               gTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGcc                                                                                                                                                                   <  1:349347/300‑1 (MQ=255)                                                                                                                                 cagcagCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACCGGCGTGGCTTGTTAGCCtt                                                                                                                                                                 >  1:216350/1‑300 (MQ=255)                                                                                                                                 cagcagCGTGGCGCGAGCCGACTCAATGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCATGGGATAATCGCCAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCATACCTGGAATACCGTCAGATCACCGATGAACTGATGAATCAACCGGCGATGACTGTCAATAACTTTGTTCGCGCTGACCCCACGCTTCCTCCCGCTCGCACGTTGCAATCTCGTTTCATCAATGAACTGGCGCGGAGTGAAGACTGGCGTGGCTTGTTAGcatt                                                                                                                                                                 >  2:255789/1‑297 (MQ=255)                                                                                                                                      gcGTGGCGCGAGTCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGcc                                                                                                                                                            <  2:463076/300‑1 (MQ=255)                                                                                                                                           gcgcGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGaa                                                                                                                                                       <  2:487507/300‑1 (MQ=255)                                                                                                                                           gcgcGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTAAGCGCAAATCAAGCAGGCATGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGAATGAAAGATTATCCACTTTATCCATAACTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTACTCCCGCTCGCACGTTGCAGTCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTCGCCTTTAGCCCAGaa                                                                                                                                                       >  2:471722/1‑300 (MQ=255)                                                                                                                                            cgcgAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGaaa                                                                                                                                                      <  2:117305/300‑1 (MQ=255)                                                                                                                                                     aCTCACTGGATAAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGaa                                                                                                                                             <  2:569996/300‑1 (MQ=255)                                                                                                                                                     aCTCACCGTATGAGCAGCGTAGCCGTTACGCGCAAATCAACCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGaa                                                                                                                                             <  2:741747/300‑1 (MQ=255)                                                                                                                                                      cTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGANTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTCGCCTTTAGCCCGGCAAAGCCCGGAAc                                                                                                                                            >  2:205805/1‑300 (MQ=255)                                                                                                                                                      cTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCGAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGTAAAAGCCCGGAAc                                                                                                                                            <  2:60981/300‑1 (MQ=255)                                                                                                                                                             gATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCACGGAACTACCGaa                                                                                                                                     >  2:317141/1‑300 (MQ=255)                                                                                                                                                             gATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTNCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGaa                                                                                                                                     >  2:756209/1‑300 (MQ=255)                                                                                                                                                             gATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTAACTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGaa                                                                                                                                     >  2:563221/1‑300 (MQ=255)                                                                                                                                                              aTGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAg                                                                                                                                    <  2:488532/300‑1 (MQ=255)                                                                                                                                                                     gcGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCNATGACCTGATGAATCAACCGGCGGTGNCTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATg                                                                                                                             >  2:579119/1‑300 (MQ=255)                                                                                                                                                                     gcGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTNCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATg                                                                                                                             >  1:638141/1‑300 (MQ=255)                                                                                                                                                                     gcGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATg                                                                                                                             >  1:6317/1‑300 (MQ=255)                                                                                                                                                                     gcGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTAACTGGAATACCGTCAGATCACCGATGACCTGATGAATCAAACGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCACGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCACGGAAAAGCCCGGAACTACCGAAGCGTAATg                                                                                                                             >  2:408879/1‑300 (MQ=255)                                                                                                                                                                     gcGTAGCCGTTACGCGCAAATCAAGCAGGACTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCAACGATGAACTGATGAATCAACCGGCGGTGCCTGTCACTAACTTTGTTCGCGCTACCCCCACGCTTCCTACCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAAATGGCGCGGCGTGAAGACTGGTGTGGCTTGTTAGCCTTTAGCCCGGAAACGCCAGGAACTACCAAAGCGCAATg                                                                                                                             >  2:231757/1‑300 (MQ=255)                                                                                                                                                                                        aTCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCaaaa                                                                                                          >  1:548091/1‑300 (MQ=255)                                                                                                                                                                                                ggCCTGTGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTTGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCGTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAacac                                                                                                  <  2:88245/300‑1 (MQ=255)                                                                                                                                                                                                ggCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGNCTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGNTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTAATATGCAAAATGGAacac                                                                                                  >  2:702475/1‑300 (MQ=255)                                                                                                                                                                                                ggCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCNTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAacac                                                                                                  >  1:590034/1‑300 (MQ=255)                                                                                                                                                                                                ggCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAacaa                                                                                                  >  2:448851/1‑299 (MQ=255)                                                                                                                                                                                                ggCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAAATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAacac                                                                                                  <  1:795462/300‑1 (MQ=255)                                                                                                                                                                                                ggCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATACCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAacac                                                                                                  <  2:516559/300‑1 (MQ=255)                                                                                                                                                                                                     gggATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCANCCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGc                                                                                             <  1:629119/300‑1 (MQ=255)                                                                                                                                                                                                     gggATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGTCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGc                                                                                             <  2:48340/300‑1 (MQ=255)                                                                                                                                                                                                      ggATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCNCTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTNAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCACGGAACTACCGAAGCGCAATGTAATTCCTACTATGCAAACTGGAACACCGGGCa                                                                                            >  2:689017/1‑300 (MQ=255)                                                                                                                                                                                                               aCAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTgaaga                                                                                   <  2:332075/300‑1 (MQ=255)                                                                                                                                                                                                               aCAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACACCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTgaaga                                                                                   <  1:773302/300‑1 (MQ=255)                                                                                                                                                                                                               aCAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTANCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCCAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTgaaga                                                                                   <  2:641908/300‑1 (MQ=255)                                                                                                                                                                                                                  aaTGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCNATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATNCAAAATGGAACACCGGGCAGAGTGAAGAAGc                                                                                <  2:619561/300‑1 (MQ=255)                                                                                                                                                                                                                   aTGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGcc                                                                               <  1:581902/300‑1 (MQ=255)                                                                                                                                                                                                                               gAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCg                                                                   >  2:565374/1‑300 (MQ=255)                                                                                                                                                                                                                                         tgCCTGGACTGAAAGATTATCCACTTTATCCNTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCNGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCtgt                                                         >  2:628721/1‑300 (MQ=255)                                                                                                                                                                                                                                         tgCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCCGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCtgt                                                         >  2:381791/1‑300 (MQ=255)                                                                                                                                                                                                                                                 ctgcaagATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCAATAACTCTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCACTGCAATGGCGCGGCTTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGTAACTCCCGAAGCGCAACGTCATTAATAATATGCAAAATGGAACCCTGGGCAGAGTGAAGAAGCCTGGCCAGGGGCTGAAGAGCTGTGGCTAAcc                                                 >  2:277329/7‑300 (MQ=255)                                                                                                                                                                                                                                                      agATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGTTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTNCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCaa                                            <  2:754163/299‑1 (MQ=255)                                                                                                                                                                                                                                                                     tCCCTACCTTGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGc                             <  2:416326/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     tCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGc                             <  2:365299/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     tCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGc                             <  1:124289/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     tCCCTACCTGGAAGACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAGCTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACGGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCTAAGCGCAATGTAATTACTACGATGCAAAATGGAACACCGGGCAGAGTGAATAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGc                             <  2:40353/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         tACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCtgt                         >  2:485873/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          aCCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTCGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAAAACCGGGCAGAGTTAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTCACGACtgtg                        >  2:674940/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          aCCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCtgtg                        >  1:747818/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           ccTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGa                       >  1:296022/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    ccgtcAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTAtt              >  1:500301/5‑300 (MQ=255)                                                                                                                                                                                                                                                                                       gcAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAg           <  2:392061/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                          gATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGATTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCCATGTAATTACTACTATGCAAAATGGAACACCGGGCAGGGTGAAGAAGCCCGGCAAGGGGAGAAAGAGCTGTGGCTAACCGGAAAGAGCAAGCCTCACGCCTGTGAAAAGTTATTTAgtgt        >  2:527900/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                              aCCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGTAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTgg    >  2:304793/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                cGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGgcg  <  1:807391/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                      GGTTAGCATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCG  >  NC_000913/4630667‑4631253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑