Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,517,127 C→A V197V (GTG→GTT)  fecR ← ferric citrate regulator FecR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,517,1270CA98.8% 289.2 / NA 84V197V (GTG→GTT) fecRferric citrate regulator FecR
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (33/50);  total (34/50)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCC  >  NC_000913/4516831‑4517422
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                       
aTAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACg                                                                                                                                                                                                                                                                                                      >  2:735460/1‑300 (MQ=255)
 tAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGt                                                                                                                                                                                                                                                                                                     <  1:180502/300‑1 (MQ=255)
  aaCGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACATCCAGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACTCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAATTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGTGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTc                                                                                                                                                                                                                                                                                                    >  2:829647/1‑300 (MQ=255)
     gTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCAGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAg                                                                                                                                                                                                                                                                                                 >  2:254218/1‑300 (MQ=255)
     gTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCAGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTACACAGCGTGCTGATGAACGTCAAg                                                                                                                                                                                                                                                                                                 >  2:840552/1‑300 (MQ=255)
     gTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCAGCTCAGGCGCAGCCAGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACNCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTACACAGCGTGCTGCTGAACGTCAAg                                                                                                                                                                                                                                                                                                 >  2:205385/1‑300 (MQ=255)
      ttCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGc                                                                                                                                                                                                                                                                                                <  1:706519/300‑1 (MQ=255)
      ttCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGc                                                                                                                                                                                                                                                                                                <  2:239926/300‑1 (MQ=255)
                                       gtaaagatCAGGCGCAGCCTGGCAACGGCGGGATCGCAGCGCAGCACGCAGTTGCGGTAGCGGGTTAGCGTGGCTATCACCTAAACCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCGTCCAGCGTTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTAACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGTTGCTGCACGTACAGCTGCGTGAAGTTATTATGCTAGCGGACGGTAAAt                                                                                                                                                                                                                                                               >  2:738865/8‑300 (MQ=255)
                                       gTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAAt                                                                                                                                                                                                                                                               >  1:270450/1‑300 (MQ=255)
                                                     cAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGTGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTNCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCNGCTGGCGGACGGTAAATTCTGTCCCTAAAGc                                                                                                                                                                                                                                                 <  2:622783/300‑1 (MQ=255)
                                                     cAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGc                                                                                                                                                                                                                                                 <  2:520304/300‑1 (MQ=255)
                                                            gcagcggcggGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCATAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCGAAAGCAGTGAGc                                                                                                                                                                                                                                          <  1:446255/296‑1 (MQ=255)
                                                            gCAACTGCGGGATCGCAGCGCAGCACGCCGTTGCAGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGc                                                                                                                                                                                                                                          <  2:472094/300‑1 (MQ=255)
                                                                cggcggGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGc                                                                                                                                                                                                                                      >  1:506828/1‑300 (MQ=255)
                                                                 tgcggGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGcc                                                                                                                                                                                                                                     <  2:477284/299‑1 (MQ=255)
                                                                  gcggGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCGCCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCAAAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGccc                                                                                                                                                                                                                                    >  2:682018/1‑300 (MQ=255)
                                                                  gcggGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCTAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGccc                                                                                                                                                                                                                                    <  1:388505/300‑1 (MQ=255)
                                                                  gcggGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGccc                                                                                                                                                                                                                                    <  1:387717/300‑1 (MQ=255)
                                                                   cggGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCATAAAGCAGTGAGCTGGCCCt                                                                                                                                                                                                                                   >  1:366670/1‑300 (MQ=255)
                                                                      gATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGAc                                                                                                                                                                                                                                <  1:806953/300‑1 (MQ=255)
                                                                       aTCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCANCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGNGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACg                                                                                                                                                                                                                               <  2:691068/300‑1 (MQ=255)
                                                                       aTCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCATAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACg                                                                                                                                                                                                                               <  2:454636/300‑1 (MQ=255)
                                                                       aTCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCANCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACg                                                                                                                                                                                                                               <  1:641733/300‑1 (MQ=255)
                                                                        tCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACgg                                                                                                                                                                                                                              >  1:699667/1‑300 (MQ=255)
                                                                         cgcagcgcagCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACggg                                                                                                                                                                                                                             <  1:709265/300‑1 (MQ=255)
                                                                                cagcaCGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGac                                                                                                                                                                                                                      >  1:719615/1‑300 (MQ=255)
                                                                                cagcaCGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTGTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGac                                                                                                                                                                                                                      <  2:106506/300‑1 (MQ=255)
                                                                                cagcaCGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAACTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCNCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGac                                                                                                                                                                                                                      <  2:222938/300‑1 (MQ=255)
                                                                                     cGCCGTTGCGTTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGa                                                                                                                                                                                                                 <  2:373418/300‑1 (MQ=255)
                                                                                        cGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAgg                                                                                                                                                                                                              <  1:60787/300‑1 (MQ=255)
                                                                                            gtggTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTCTCCTGCTGGCGGACGGTAAATTCTGTCCATAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCg                                                                                                                                                                                                          >  1:416314/3‑300 (MQ=255)
                                                                                              ggTAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATNCTGTCCCTAAAGCAGTGAGATGGCCCTGACGGGTCAGGACACGGAAGGGGCGtt                                                                                                                                                                                                        >  2:654126/1‑300 (MQ=255)
                                                                                                tAACGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGc                                                                                                                                                                                                      <  1:408567/300‑1 (MQ=255)
                                                                                                  aCGGGTTAGCGTTGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCATCGGTTTCACTGCGCCAAACTCATAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCTTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAg                                                                                                                                                                                                    <  2:450324/300‑1 (MQ=255)
                                                                                                  aCGGGTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAg                                                                                                                                                                                                    <  1:95883/300‑1 (MQ=255)
                                                                                                     ggTTAGCGTTGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCAGCCAGCGGTTTCACTGCGCCAAACTCATAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACAGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGc                                                                                                                                                                                                 <  1:706526/300‑1 (MQ=255)
                                                                                                     ggTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGc                                                                                                                                                                                                 <  1:706832/300‑1 (MQ=255)
                                                                                                     ggTTAGCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGc                                                                                                                                                                                                 <  2:782963/300‑1 (MQ=255)
                                                                                                          gcggtgCTATCACCTCACCCAGTGGGTTATCGCTGAAGCTCATGATGTCCTTCGTCCAGCTTGTACTCTCGTCATTCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCATGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGTGCATCtt                                                                                                                                                                                            <  2:284135/295‑1 (MQ=255)
                                                                                                          gCGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTAAGGACACGGAAGGGGCGTTGCAGGGCATCtt                                                                                                                                                                                            >  2:427339/1‑300 (MQ=255)
                                                                                                           cGTGGCTATCACCTCACCCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCttt                                                                                                                                                                                           <  1:840946/300‑1 (MQ=255)
                                                                                                               gCTATCACCTCACCCAGTAGTTTACCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCACACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGNGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCAAAGCGTGCTGCTGANCGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCATAAAGCAGTGAGCTGCCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTCcgcg                                                                                                                                                                                       >  2:697918/1‑300 (MQ=255)
                                                                                                                    cacctcaccCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCATTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGgtagt                                                                                                                                                                                  >  2:837150/1‑300 (MQ=255)
                                                                                                                         caccCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATgg                                                                                                                                                                             <  1:536498/300‑1 (MQ=255)
                                                                                                                          accCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGNAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGc                                                                                                                                                                            >  1:649015/1‑300 (MQ=255)
                                                                                                                          accCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGc                                                                                                                                                                            <  1:532737/300‑1 (MQ=255)
                                                                                                                          accCAGTGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGAGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGc                                                                                                                                                                            >  2:155089/1‑300 (MQ=255)
                                                                                                                                   tttATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCATAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCTAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCAcc                                                                                                                                                                   <  1:107899/300‑1 (MQ=255)
                                                                                                                                              aGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCATAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGcc                                                                                                                                                        >  2:548234/1‑300 (MQ=255)
                                                                                                                                               gCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTACCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCCTCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCg                                                                                                                                                       >  2:244614/1‑300 (MQ=255)
                                                                                                                                               gCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCAACAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTACTGGCGATTTCACCGTACCAGAGCCg                                                                                                                                                       >  2:833032/1‑300 (MQ=255)
                                                                                                                                                  cAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCATAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGAc                                                                                                                                                    <  1:150959/300‑1 (MQ=255)
                                                                                                                                                  cAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGATAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGAc                                                                                                                                                    <  1:651405/300‑1 (MQ=255)
                                                                                                                                                  cAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGAc                                                                                                                                                    <  1:497783/300‑1 (MQ=255)
                                                                                                                                                  cAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGAc                                                                                                                                                    <  2:787226/300‑1 (MQ=255)
                                                                                                                                                            ttCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGa                                                                                                                                          <  1:541434/300‑1 (MQ=255)
                                                                                                                                                            ttCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGa                                                                                                                                          <  2:342739/300‑1 (MQ=255)
                                                                                                                                                            ttCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTGCACTGCGCCAAACTCAGAGGCGCTGAACTGCATGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGa                                                                                                                                          <  2:735542/300‑1 (MQ=255)
                                                                                                                                                                   aGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCAt                                                                                                                                   >  2:539283/1‑300 (MQ=255)
                                                                                                                                                                   aGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTAAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCAt                                                                                                                                   >  1:278598/1‑300 (MQ=255)
                                                                                                                                                                   aGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCATAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCAt                                                                                                                                   >  2:347502/1‑300 (MQ=255)
                                                                                                                                                                     cTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCa                                                                                                                                 >  1:534248/1‑300 (MQ=255)
                                                                                                                                                                         tACTCTCTTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAAc                                                                                                                             <  1:472448/300‑1 (MQ=255)
                                                                                                                                                                                     ccAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATccgccg                                                                                                                 <  1:372011/300‑1 (MQ=255)
                                                                                                                                                                                         cGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGAGCt                                                                                                             >  2:242933/1‑300 (MQ=255)
                                                                                                                                                                                               cACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGt                                                                                                       <  1:732408/300‑1 (MQ=255)
                                                                                                                                                                                                          aCTCATAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGa                                                                                            <  1:381684/300‑1 (MQ=255)
                                                                                                                                                                                                          aCTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGa                                                                                            <  1:381655/300‑1 (MQ=255)
                                                                                                                                                                                                            tCAGATGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCTTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGc                                                                                          <  2:828478/300‑1 (MQ=255)
                                                                                                                                                                                                            tCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGc                                                                                          <  1:878394/300‑1 (MQ=255)
                                                                                                                                                                                                                  gcgcTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGAGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATGCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGAGCTTTGGGTATTCAGCGTGAGCAggaag                                                                                    <  1:155820/300‑4 (MQ=255)
                                                                                                                                                                                                                          cTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTc                                                                            >  1:396745/1‑300 (MQ=255)
                                                                                                                                                                                                                               ggCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGCAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGGAATCATCCTGCTGGCGGACGGTGAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGGCAAGGAAGGGGCGTTGCAGGGCATCTTTCGCAGTAGTAGTGGCGATTTCACCGTACAAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGACGCGCTTTGGGTATTCCGCGTGGGCAGGGAGcaccctcacggtt                                                                       >  2:232565/1‑288 (MQ=255)
                                                                                                                                                                                                                                gCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTACACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTACCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCAGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTg                                                                      >  2:105012/1‑300 (MQ=255)
                                                                                                                                                                                                                                                   aTGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCt                                                   <  1:271611/300‑1 (MQ=255)
                                                                                                                                                                                                                                                        tttttGCGCGGGGGCACTGGCTAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGgc                                              <  1:206503/300‑1 (MQ=255)
                                                                                                                                                                                                                                                        tttttGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCCGGGCATCTTTCGCGGTAGTAATGGCGNTTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTNCCAGTTGCTGACGGCTGACTGTGCCTTTGgc                                              <  2:612627/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                    ggCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGANGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATc                                  <  1:637863/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                    ggCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATc                                  <  1:380527/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                     gCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCt                                 >  1:677306/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                          ggCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCATAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCg                            >  1:783722/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                          ggCGAGAAGCACTTCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCATAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGAGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCg                            >  1:784200/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                       tCCACAGCGTGCTGCTGAACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACNCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCg               >  1:605774/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                    gctgaACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGcc  >  2:839443/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                       
ATAACGTTCAGGATCGCATCGGTATTTTTCAGCGGGAACGTCCCGCTCAGGCGCAGCCCGGCAACGGCGGGATCGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGACACGGAAGGGGCGTTGCAGGGCATCTTTCGCGGTAGTAATGGCGATTTCACCGTACCAGAGCCGGACGGTGCGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCC  >  NC_000913/4516831‑4517422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑