Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,516,378	G→A	N100N (AAC→AAT)	fecA ←	ferric citrate outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,516,378	0	G	A	98.6%	239.4 / NA	71	N100N (AAC→AAT)	fecA	ferric citrate outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (43/27);  minor base C (1/0);  total (44/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGTGTTAACCAAAGGTGTTGTTTTACGAAAAAC  >  NC_000913/4516083‑4516662                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                             cGTCCATCAGGACGGTCGAGCGGCTGGTGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCtattat                                                                                                                                                                                                                                                                                          <  1:470247/300‑1 (MQ=255)     cATCAGGACGGTCGAGCGGCTGGTGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTcc                                                                                                                                                                                                                                                                                      >  1:560102/1‑300 (MQ=255)              ggTCGAGCGGCTGGTGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGttt                                                                                                                                                                                                                                                                             >  1:152393/1‑300 (MQ=255)               gTCGAGCGGCTGGTGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGtttt                                                                                                                                                                                                                                                                            >  1:365653/1‑300 (MQ=255)               gTCGAGCGGCTGGTGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCAACCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTACTTACCAGCGGtttt                                                                                                                                                                                                                                                                            >  2:785053/1‑300 (MQ=255)                  gAGCGGCTGGTGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTAcc                                                                                                                                                                                                                                                                         <  2:71539/300‑1 (MQ=255)                                     ggTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCANATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTCTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCca                                                                                                                                                                                                                                                      >  2:195702/1‑300 (MQ=255)                                     ggTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCca                                                                                                                                                                                                                                                      >  1:826702/1‑300 (MQ=255)                                            ccctgcgagatgtgtataagagacagggTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAgttg                                                                                                                                                                                                                                               <  2:547958/274‑1 (MQ=255)                                               ccGGATGCCAAAGTTCATCGCCAGGGCGTGGCTGCCGGTGCCGTTGTTTTTCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATAGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGttgttg                                                                                                                                                                                                                                            <  2:322137/300‑1 (MQ=255)                                                 ggATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCa                                                                                                                                                                                                                                          <  1:129620/300‑1 (MQ=255)                                                  gATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGNCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAg                                                                                                                                                                                                                                         <  1:635393/300‑1 (MQ=255)                                                    tGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCTGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGc                                                                                                                                                                                                                                       <  1:166279/300‑1 (MQ=255)                                                           gTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCtctc                                                                                                                                                                                                                                >  1:75796/1‑300 (MQ=255)                                                                tCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGAcgt                                                                                                                                                                                                                           >  1:672309/1‑300 (MQ=255)                                                                tCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCAGGCTCAAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCAGATCTCGAcgt                                                                                                                                                                                                                           >  2:182842/1‑300 (MQ=255)                                                                tCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTAACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCAAGCATGTTCAAATACGTCGTTTTCACGCGCATCACC‑AGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTACCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGAagtc                                                                                                                                                                                                                          >  2:256442/1‑300 (MQ=255)                                                                 cGCCATGTCTTGGCTGCCGGTGCCGTTGTGTTCCGGCGCGCTGACGCCATGGATGCGGTTAAGTACCTCACGCATGGTGGTGGCGCCGGTTTTGTCGAAATCCTCACTGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGAcgtc                                                                                                                                                                                                                          <  2:400617/300‑1 (MQ=255)                                                                      ggTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAgttgctgtctcttatacacatctccgagcc                                                                                                                                                                                                                     >  1:559220/1‑274 (MQ=255)                                                                      ggTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAAt                                                                                                                                                                                                                     >  1:117882/1‑300 (MQ=255)                                                                          gTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTNTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGcc                                                                                                                                                                                                                 >  1:628304/1‑300 (MQ=255)                                                                                   ggTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGcc                                                                                                                                                                                                        <  2:536825/300‑1 (MQ=255)                                                                                            gttTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACAGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCAAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCATGCCGCTCTCGACGTCGTAATCGACGTGCAAGCCGTTGCTCTg                                                                                                                                                                                               >  2:229899/1‑300 (MQ=255)                                                                                                       cgcTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCcgcgcg                                                                                                                                                                                    >  1:231777/1‑300 (MQ=255)                                                                                                        gcTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGt                                                                                                                                                                                   >  1:706766/1‑300 (MQ=255)                                                                                                             cGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGTTTTTGGCGAAATCCTCACTGCGGATCACGTCACGCGCACCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGc                                                                                                                                                                              <  2:259272/300‑1 (MQ=255)                                                                                                                   ggATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGt                                                                                                                                                                        <  1:548339/300‑1 (MQ=255)                                                                                                                        cGGTTCAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAACCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTCCTCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAAcc                                                                                                                                                                   >  2:170679/1‑300 (MQ=255)                                                                                                                        cGGTTAAGTACCTCACGCATGGTGGTTGCGCNGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGANCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTCATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAAcc                                                                                                                                                                   >  2:608386/1‑300 (MQ=255)                                                                                                                        cGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAAcc                                                                                                                                                                   >  1:131092/1‑300 (MQ=255)                                                                                                                          gTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCga                                                                                                                                                                 >  1:319586/1‑300 (MQ=255)                                                                                                                          gTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGAAGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCga                                                                                                                                                                 >  2:836932/1‑300 (MQ=255)                                                                                                                            tAAGTACCTCACGCATGGTGGTTGTGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCAAGCATGTTCAAATACGTCGTTTTCACGCGCATCACACAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCCGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCgaga                                                                                                                                                               >  2:254781/1‑300 (MQ=255)                                                                                                                            tAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCgaga                                                                                                                                                               >  1:326236/1‑300 (MQ=255)                                                                                                                                  ccTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCANGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCAGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGNGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTaa                                                                                                                                                         >  2:808044/1‑300 (MQ=255)                                                                                                                                       cGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCg                                                                                                                                                    >  2:559479/1‑300 (MQ=255)                                                                                                                                           tggtggTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCtgt                                                                                                                                                >  1:736719/1‑300 (MQ=255)                                                                                                                                               ggtTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGNATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCNGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTACTCTGCTTGCCGCGCGTCAGGCTGGCGGCAACCGAGAGGGTAAATCCGCTGTGTGc                                                                                                                                            <  2:687782/300‑1 (MQ=255)                                                                                                                                                  tGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGc                                                                                                                                         <  1:38818/300‑1 (MQ=255)                                                                                                                                                    cgtcGGTTTTGGCGAAATCCTCACGGCTGATCACGTCACGCGCGCCAGCATGGTCAAATGCGTCTTTTTCACGCGCATCACCCAGCCATTCACCGACCACGGTCAGGGCATCTTCTTTTTGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCCGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTTTTGCATGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCTTTGCTCTGCTTTCCGTGTGGTAAGATGGCGACAACCGAGAGGGTAAATCCGCTGTGTGCGGCAt                                                                                                                                       <  2:408725/297‑1 (MQ=255)                                                                                                                                                    cgcCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCAt                                                                                                                                       <  1:525234/300‑1 (MQ=255)                                                                                                                                                      ccGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATAc                                                                                                                                     <  1:565112/300‑1 (MQ=255)                                                                                                                                                                      ccTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACNCGCATCACCCAGCCAGTCGCCGACCACCGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGGCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTAt                                                                                                                     <  2:647193/300‑1 (MQ=255)                                                                                                                                                                                     cgtcactCGCGCCAGCATGTCCAAAGACTTCGTTTCCACGCGCATCACCCAGCCAGTCGCCGACCACTGTCAGGGCATCTTCCTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTGCTACCTGCAGTCCGCTACCTTCCATCATTTGTTACATTCCGCCCTCGACGCCATCCTCACCGCGCCTTCCGCTTCTCTGCTTCCCGTGCGGCAGGCCGGCGGCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTg                                                                                                      <  2:429416/300‑1 (MQ=255)                                                                                                                                                                                     cgtcacgCGCGCCAGCATATTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGTTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCATGCCGTTGCTCTGCTTTCCGCGCGTCAGGCTGGCGGCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTg                                                                                                      <  2:184390/300‑1 (MQ=255)                                                                                                                                                                                              cgcCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTaa                                                                                             >  1:787058/1‑300 (MQ=255)                                                                                                                                                                                                ccAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACTACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGTTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAAGCCTCTGCTCTACTTGCCGCGCACCAGGCTGGCGGCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAAcc                                                                                           <  2:195045/300‑1 (MQ=255)                                                                                                                                                                                                ccAGCATGTTCAAATACGTCATTTTCACGCGCATCACCCAGCCAGTCGCCGACCACAGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGTTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCATGCCGTTGCTCTGCTTTCCGCGTGTCGGGCTGGCGGCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAAcc                                                                                           <  2:228028/300‑1 (MQ=255)                                                                                                                                                                                                ccAGCACGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCGCGGTCATTGCATCTTCTTTTGGTACGGGCGCGGGCTCCAGCGTTCAGCTATTATTTCCCAGCGGTTTTACCTACAGTNCACTGCCACCCAGCAGTCTTTACAGTCCGCCCTCTACGTCATAATCCCCGTGCATGCCGTTGCTCCGCTTGCCGCGCGGCAGGCCGGCGGCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAAcc                                                                                           <  2:207033/300‑1 (MQ=255)                                                                                                                                                                                                    cATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCtgtg                                                                                       >  1:343700/1‑300 (MQ=255)                                                                                                                                                                                                       gTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGACGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGCTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGTGTTATCGAGCGATCCCGGTGCGATGTTAACCTGTgcag                                                                                    >  2:391204/1‑300 (MQ=255)                                                                                                                                                                                                          cAAATACGTCGTTTTCACGCGCATCACCCAGCCATTCGCCGACCACAGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGGCAGGCTGGCGGCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCaa                                                                                 <  2:376793/300‑1 (MQ=255)                                                                                                                                                                                                          cAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCaa                                                                                 <  1:475448/300‑1 (MQ=255)                                                                                                                                                                                                                             cgcATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCAGACTGATTGAGCGTCTTCTCGAGCGATCCCGGTGTGATACTACCCTGTGCAGCAAAAGCGGAAAAAGAGATAcc                                                              >  2:134884/1‑300 (MQ=255)                                                                                                                                                                                                                                 tCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATAATGATTGAGCGCTTTATCGAGNGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGNCCGGcc                                                          >  2:717502/1‑300 (MQ=255)                                                                                                                                                                                                                                  cACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGTTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGTTGCGATATTAAACTGTGCAGCAAAAGCGGAAAACGAGAGACCGGcaa                                                         >  2:269207/1‑298 (MQ=255)                                                                                                                                                                                                                                  cACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCNACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCa                                                         >  1:594942/1‑300 (MQ=255)                                                                                                                                                                                                                                        gccagTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGgca                                                   >  1:444332/1‑300 (MQ=255)                                                                                                                                                                                                                                            gTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGcagcag                                               >  1:665315/1‑300 (MQ=255)                                                                                                                                                                                                                                               gCCGACCACGTTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCt                                            <  1:339718/300‑1 (MQ=255)                                                                                                                                                                                                                                                       cGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGaa                                    >  1:39440/1‑300 (MQ=255)                                                                                                                                                                                                                                                       cGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGNCAACCGAGAGGGTAAATCTGCTGTGTGCGGCATATTGATCGAGCGCGTTATCAAGCGATCCCGGCGCGATATTACCCTTTGTAGCAAAAGCGGAAAACGAGAGACCGGTCAGCGGCAGTAGGATAAGGCgga                                    >  2:218050/1‑298 (MQ=255)                                                                                                                                                                                                                                                        ggTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCCAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAAt                                   >  1:431521/1‑300 (MQ=255)                                                                                                                                                                                                                                                        ggTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAAt                                   >  1:229288/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  tcttctTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAAcc                         <  1:808950/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  tcttctTTTGGTGCGGGCGCGGGCTCCAGCGCCCAGCTATTATTTCCCAGCGTTTTTACCTGCAGTCCGCTGCCGTCCAGCGGTTGTTGCAGTCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCCTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAAcc                         <  2:87600/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    ttcttTTGGTGCGGGCGCGGGCTCCAGCGTCCCGTTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAATTGTTGCAGGCCGCTCTAGACGTCGTAATCGCAGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGTATACTGATTGAGTGCTTTTTCGAGCGATCACGGTGCGATATCAACCTGTGCAGCAAAAGCGGAAAACGAAAGACCGGCAAGCGGAAGCAGGACCAGGAGAATGGCGTTAACCaa                       >  2:167192/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       ttttGGTGCGGGCGCGGGCTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACTTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAgg                    <  2:567002/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 ggcgcgggcTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTa          >  1:685313/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       ggcTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGTGATCCCGGTGCGATATTAACCTGTGTAACAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAGTGGCGTTAACCAAAGGTGTTGTTTTACGaaaa    >  2:303489/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         cTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGCGTTAACCAAAGGTGTTGTTTTACGAAAAAc  >  1:17328/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         cTCCAGCGTCCAGCTATTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGAAGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCGTTATCGAGTGGTTCAGGTGTGATAGTGGCCTGTGCAGCAAAAGTGGAAAACGAGAGAACGGCCAGTGGCAGCAGGCTAAGGCGAATGGTGTTAACCAAAGATGTTGTTCTATGAAAAAc  >  2:475173/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                             CGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTAACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGTGTTAACCAAAGGTGTTGTTTTACGAAAAAC  >  NC_000913/4516083‑4516662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑