| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,512,642 | T→C | V256V (GTA→GTG) | fecC ← | ferric citrate ABC transporter membrane subunit FecC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,512,642 | 0 | T | C | 98.0% | 169.0 / ‑6.1 | 51 | V256V (GTA→GTG) | fecC | ferric citrate ABC transporter membrane subunit FecC |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (23/27); minor base A (1/0); total (24/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGAC > NC_000913/4512354‑4512933 | gTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGacgca > 2:776366/1‑300 (MQ=255) aaCAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACgcacgcac < 1:822191/300‑1 (MQ=255) gcagctcgctagtacagttgctagtaaaaTAATCAGCGTAATCTTCATCCTCGCCTCCTCACAATCCATACAAAGCAGGGGCTGCCAATCAGCGCCAGCACTTCGCCTGCAGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTGCAGCTGCCAGCAGCATCAGCGTGGCCTCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCATAAGCGCACCAGATGTTGCACCAGCAGACCGATAAACACCACCGGGCCTGCCACGCTGACGCACGCACCAACCAGa < 2:24287/275‑1 (MQ=255) aGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCCCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCANATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCNGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAag < 2:696850/300‑1 (MQ=255) gCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAagc < 1:376202/300‑1 (MQ=255) aGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACatat < 1:695912/300‑1 (MQ=255) gatgaAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATtgatg < 1:507118/300‑1 (MQ=255) aaaaTAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCa > 2:123526/1‑300 (MQ=255) aGCGCANTTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAANCAGCGCCAGCACTGCGCCTGCGGGCAGACCTCCGTGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTatcc < 2:722588/300‑4 (MQ=255) cTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTc < 2:494368/300‑1 (MQ=255) cctcctCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAAAAGCGCGCCAGCTGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCATGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTAGCCAGGTTCACTCCCAg > 2:265074/1‑300 (MQ=255) caAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATggg < 1:849718/300‑1 (MQ=255) aaGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGc < 1:207347/300‑1 (MQ=255) aGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACNGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACNGATAAACGCCACCAGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGGTGACCAAACGTAGCATCGTCAGGTTCACTCCCAGCGTATGGGCGGTGc > 2:694509/1‑300 (MQ=255) aaGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCg > 1:50749/1‑300 (MQ=255) aaGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTACGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCATAGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCg > 2:468275/1‑300 (MQ=255) aGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGc < 2:839108/300‑1 (MQ=255) gcgcCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTNACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCACAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCa > 1:649476/1‑300 (MQ=255) cgcCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAg > 2:425806/1‑300 (MQ=255) cgcCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAAAGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAg > 2:51703/1‑300 (MQ=255) cgcCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCACAGCAGCATGCTCACTGGCAGTATGTTGCGCTGATCGAAGACTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGAACCTGCAAAGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTAGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCGAGTTCAg > 1:57500/1‑300 (MQ=255) aTCTCCGTGGAAGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGAc < 2:334576/300‑1 (MQ=255) aGGCCAGTGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAg < 2:786854/300‑1 (MQ=255) cgTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACAAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCCGGTTCAGTTGATTCGCCAGCAGCAAAACGACAGGGACTGCAGTGagcacaacc > 1:369243/1‑300 (MQ=255) gCGTGGCCCCCAGCCGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCCGACATCCTGCCaac > 2:568074/1‑298 (MQ=255) gCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCg > 1:191161/1‑300 (MQ=255) gCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCACAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAACCGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGAAAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCg > 2:246087/1‑300 (MQ=255) ccccAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCAACCGGACCTGCCACGCTGACGCACGCACCAAACAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCANGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGAAAGGGACTGCAATGACCACCAACGGCCAGAGCTGACAGACATCCTGCCAGCGGGcatg > 2:209597/1‑297 (MQ=255) cagcagCATGCTCACTGGCGATACGTTGCGCTGATCGAAGCCTGCCCATAAACGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGATCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGa < 2:262262/300‑1 (MQ=255) cagcagCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTggta < 2:261706/300‑3 (MQ=255) cagcaaCATGCTCACTGGCAGTACGTGGCGCTGATCGAGGCCTGCCCATAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGTACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCGCTAACATAGTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGGGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAGCACGACAGGGACTGCAGTGACCACCACCTGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGGGGGa < 2:261771/300‑1 (MQ=255) agcagcATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACTTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTTGGac < 2:161047/300‑1 (MQ=255) agcagcATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGac < 1:177736/300‑1 (MQ=255) gcaTGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGAGGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTc < 1:261562/300‑1 (MQ=255) caTGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTcc < 1:148456/300‑1 (MQ=255) gCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTgccagcc > 1:12611/1‑300 (MQ=255) aCGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTaa > 1:655607/1‑300 (MQ=255) ttGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAg < 1:727515/300‑1 (MQ=255) gcgcTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGTCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAGCGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGAt < 2:188980/300‑1 (MQ=255) gcgcTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGAt < 2:790160/300‑1 (MQ=255) gATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGt > 2:538305/1‑300 (MQ=255) gCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCt > 1:203093/1‑300 (MQ=255) gcgcCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGNACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCcagca > 1:626503/1‑300 (MQ=255) cgcCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCAGTAAGCATGATCTTCGGCcagcag > 1:555016/1‑300 (MQ=255) gcCAGATGTGGCACCAGCAGACCGAGAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCATAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTTGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCcagcagc < 1:234385/300‑1 (MQ=255) aTGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGt < 1:18158/300‑1 (MQ=255) gACCGATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGc < 1:471071/300‑1 (MQ=255) gATAAACGCCACCGGACCTGCCACGCTGCCGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGCCAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGCGGGACACTCCGACTGCCAGACAGTAAAAGATGCAGTCAGCATGATCTTAGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCAt > 2:476211/1‑300 (MQ=255) gATAAACGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAANGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAANGCCCAt > 2:720907/1‑300 (MQ=255) cGCCACCGGACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACaaaa > 1:793637/1‑300 (MQ=255) ggACCTGCCACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTGGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCATTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGtctgtctcttatacacatctcgc < 1:724606/300‑23 (MQ=255) | GTGATAACAGCGCACAGCCCGCCAGGGCAAGGGTGATGAAAATAACCAGCGCAATTTTCATCCTCGCCTCCTCACAAGCCAGACAAAGCAAGGGCTGCCAATCAGCGCCAGCACTGCGCCTGCGGGCAGATCTCCGGGGAAGGCCAGCGCGCGTGCGAGTACATCTGCCAGCAGCATCAGCGTGGCCCCCAGCAGCATGCTCACTGGCAGTACGTTGCGCTGATCGAAGCCTGCCCAGAAGCGCGCCAGATGTGGCACCAGCAGACCGATAAACGCCACCGGACCTGCTACGCTGACGCACGCACCAACCAGAAGCAGCACTAACATATTGATGACCAAACGTAGCCTCGTCAGGTTCACTCCCAGCGTATGGGCGGTGCTGTCGCTGAGGTTGAGCAGGTTCAGTTGATTCGCCAGCAGCAACACGACAGGGACTGCAGTGACCACCACCGGCAAGAGCTGCCAGACATCCTGCCAGCGGGCGTGGGACACTCCGCCTGCCAGCCAGTAAAAGATGCCGTAAGCATGATCTTCGGCCAGCAGCAGGGTGATGCGGGTAAGGCCCATACAAAAGGCCGAC > NC_000913/4512354‑4512933 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |