Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,446,482	C→T	L791L (CTG→TTG)	tamB →	translocation and assembly module subunit TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,446,482	0	C	T	100.0%	207.6 / NA	62	L791L (CTG→TTG)	tamB	translocation and assembly module subunit TamB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/24);  total (38/24)

CCGCAATAAGGAGCAAAAAATCAGCATCGGGCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCGGGGATGGTGAGTGCCAACTTGCGTCTGGGTGGTGATGTGCAAAGCCCGCAGTTGTTTGGTCAGCTTCAGGTTACGGG  >  NC_000913/4446187‑4446777                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        ccGCAATAAGGAGCAAAAAATCAGCATCGGGNCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCNTCGACCTCGCCATGCTGAAACCGTTTGTGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGcc                                                                                                                                                                                                                                                                                                     >  2:607978/1‑300 (MQ=255)  cGCAATAAGGAGCAAAAAATCAGCATCGGGCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGccc                                                                                                                                                                                                                                                                                                    <  1:712975/300‑1 (MQ=255)                  aaattagtaTCGGGCCACACTGCTGGCTTAACTCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGAGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGacac                                                                                                                                                                                                                                                                                    <  2:66855/292‑1 (MQ=255)                            cGGGCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGAc                                                                                                                                                                                                                                                                          <  1:745913/300‑1 (MQ=255)                             gggCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACNCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAANCTGAcg                                                                                                                                                                                                                                                                         >  2:720768/1‑300 (MQ=255)                             gggCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGAcg                                                                                                                                                                                                                                                                         >  1:348326/1‑300 (MQ=255)                             gggCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGANGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACNCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGAcg                                                                                                                                                                                                                                                                         >  2:815392/1‑300 (MQ=255)                              ggCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCAGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGAcgg                                                                                                                                                                                                                                                                        >  2:678878/1‑300 (MQ=255)                                     ctgctgGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTCTCAGACACTGAATCTGACGGCGGAAtt                                                                                                                                                                                                                                                                 >  2:439456/1‑300 (MQ=255)                                     ctgctgGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCAGAAGGGCGTGCGGTGGTGAATCTAAACCGCTTCGACCTCGACATGCTGAAACCGCTTGTGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGGGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGAAGGCGGAAtc                                                                                                                                                                                                                                                                 >  2:306546/1‑299 (MQ=255)                                          ggCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACATTGAATCTGACGGCGGAATTGCGTa                                                                                                                                                                                                                                                            >  2:234496/1‑300 (MQ=255)                                          ggCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTAGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTa                                                                                                                                                                                                                                                            >  2:401218/1‑300 (MQ=255)                                           gCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCNTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTaa                                                                                                                                                                                                                                                           <  2:766980/300‑1 (MQ=255)                                               aaCCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTaacaac                                                                                                                                                                                                                                                       <  1:578757/300‑1 (MQ=255)                                                      aTGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGNAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCTGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCg                                                                                                                                                                                                                                                <  2:767755/300‑1 (MQ=255)                                                      aTGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCg                                                                                                                                                                                                                                                >  1:237611/1‑300 (MQ=255)                                                           gAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCNTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTg                                                                                                                                                                                                                                           >  1:637719/1‑300 (MQ=255)                                                              cTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTTAAACCGTTTATGCCAGAAACCACTCATGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTTGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACTGCGGAATTGCGTAACAACCGTGCCGAATTGGGc                                                                                                                                                                                                                                        <  2:354755/300‑1 (MQ=255)                                                                      gcCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCAt                                                                                                                                                                                                                                >  2:850479/1‑300 (MQ=255)                                                                              cTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGa                                                                                                                                                                                                                        >  2:477831/1‑300 (MQ=255)                                                                                                        gCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTAGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGAcag                                                                                                                                                                                              >  2:777648/1‑300 (MQ=255)                                                                                                               tgAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCGGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGAcaggtgcagg                                                                                                                                                                                       <  1:552793/300‑1 (MQ=255)                                                                                                               tgAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGAcaggtgcagg                                                                                                                                                                                       <  2:252785/300‑1 (MQ=255)                                                                                                                 aaTCTCAACCGCTTCGACCTCGCCATGCTGAAACCATTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGTTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGAcaggtacaggtg                                                                                                                                                                                     <  2:375879/300‑1 (MQ=255)                                                                                                                                    tCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGgccgcc                                                                                                                                                                  >  2:334584/1‑300 (MQ=255)                                                                                                                                          tGCTGAAACCGTTTATGCCAGAAACCACTCATGCCAGCGGTATCTTCACTGTTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATc                                                                                                                                                            <  2:273686/300‑1 (MQ=255)                                                                                                                                              gAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTtgg                                                                                                                                                        >  2:267700/1‑300 (MQ=255)                                                                                                                                                              tgtgtataagagacaggCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATc                                                                                                                                        <  1:130297/284‑1 (MQ=255)                                                                                                                                                                  ccACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGTAGATGACCGATCAGCAAGGCCGCCGTAATCTCGGTGGCAACGCAAATATCCGTa                                                                                                                                    >  2:65005/1‑300 (MQ=255)                                                                                                                                                                  caaCTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCAGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTa                                                                                                                                    >  2:232171/3‑300 (MQ=255)                                                                                                                                                                         ggCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGAGCGATCCGCAAGGCCGCCGCAATCTTGGTGGCAACGTCAATATCCGTAACTTCaa                                                                                                                             >  2:827224/1‑300 (MQ=255)                                                                                                                                                                         ggCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCaa                                                                                                                             >  2:816853/1‑300 (MQ=255)                                                                                                                                                                         ggCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCAAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCaa                                                                                                                             >  1:458215/1‑300 (MQ=255)                                                                                                                                                                            cAGCGGTATCTTCACGGGGAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACNGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTNGTGGCAACGTCAATATCCGTAACTTCAACCt                                                                                                                          <  2:611958/300‑1 (MQ=255)                                                                                                                                                                              gCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATcttgtctcttatacaca                                                                                                                        >  2:129807/1‑284 (MQ=255)                                                                                                                                                                                     cTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCgatgat                                                                                                                 >  1:723399/1‑300 (MQ=255)                                                                                                                                                                                                     gACGTTGCCTTGGACACCACGAAATATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTAcc                                                                                                 <  2:672142/300‑1 (MQ=255)                                                                                                                                                                                                     gACGTTGCCTGGGACACCACGAAAGAGGGGCTGCTGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGTTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTAcc                                                                                                 <  2:430762/300‑1 (MQ=255)                                                                                                                                                                                                     gACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCATGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGTCGAATTGGGCTGGACCATCCGCCTGACCAAGAACGGCCAGTTTGATGGACAAGTGTAGGTGACCGATCCGCAAGGCCGCCTTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTTCGATGATAAACCCCATCTTTAcc                                                                                                 <  2:280823/300‑1 (MQ=255)                                                                                                                                                                                                        gTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGt                                                                                              >  2:173398/1‑300 (MQ=255)                                                                                                                                                                                                           gCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATNCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTggg                                                                                           >  2:208163/1‑300 (MQ=255)                                                                                                                                                                                                           gCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTggg                                                                                           >  2:819310/1‑300 (MQ=255)                                                                                                                                                                                                                ggACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGaaaa                                                                                      >  2:307200/1‑300 (MQ=255)                                                                                                                                                                                                                        cGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGGGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCgg                                                                              <  1:288626/300‑1 (MQ=255)                                                                                                                                                                                                                          aaaGATGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGTTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCATACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCgggg                                                                            <  2:391855/300‑1 (MQ=255)                                                                                                                                                                                                                                      ccGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCGGGGATGGTGAGTGcc                                                                >  1:36261/1‑300 (MQ=255)                                                                                                                                                                                                                                           gggCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAANGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCGGGGATGGTGAGTGCCAACtt                                                           >  1:641301/1‑300 (MQ=255)                                                                                                                                                                                                                                           gggCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCGGGGATGGTGAGTGCCAACtt                                                           >  1:156084/1‑300 (MQ=255)                                                                                                                                                                                                                                           ggacaatATCACCCTCTCGGGGCGTAACGTGCAAGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGCATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAAACTTGCGATGATAAACCACATCCTTACCCGTGGGGGAAAAGCAGCGGGGATGGTAAGTGCCAAcct                                                           >  2:22585/7‑298 (MQ=255)                                                                                                                                                                                                                                                gTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCGGGGATGGTGAGTGCCAACTTGCGTc                                                      >  2:790158/1‑300 (MQ=255)                                                                                                                                                                                                                                                gTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCGGGGATGGTGAGTGCCAACTTGCGTc                                                      >  1:808909/1‑300 (MQ=255)                                                                                                                                                                                                                                                     gctcttgcGGGGCGTAGCGGGCAGGTAACGCAAACCGTCAACGATGCGGTGTGGCCCGTGGCGTTTCAGACATTGAATCTGACGGCGGAATGGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGGGACCGATCCGCGATGCCGCCGTAATCTTGGTGGCAACTTCAATATCTGTAACGTCAACCTGTCGAGGATAAACCCCATCTTTACCCGTGGGTAAAAAGCAGCGTGGATGGTGAGTGCCAACTTGCGTCTGggt                                                 <  2:424544/293‑1 (MQ=255)                                                                                                                                                                                                                                                     aCCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCGGGGATGGTGAGTGCCAACTTGCGTCTGggt                                                 <  2:566847/300‑1 (MQ=255)                                                                                                                                                                                                                                                        cTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCGGGGATGGTGAGTGCCAACTTGCGTCTGggtggt                                              >  1:705149/1‑300 (MQ=255)                                                                                                                                                                                                                                                          ttCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCGGGGATGGTGAGTGCCAACTTGCGTCTGGGTGGTGa                                            <  2:846452/300‑1 (MQ=255)                                                                                                                                                                                                                                                              gggCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCGGGGATGGTGAGTGCCAACTTGCGTCTGGGTGGTGAtgtg                                        >  2:662506/1‑300 (MQ=255)                                                                                                                                                                                                                                                               ggcgcagcGTGCAGTTAACACAAACCATCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGGTTGAGGGACAGGGGCAGGGGACCGATCTGCAAGGCCGTCGGAATCTTGTTGGCAACGTCAATATCCGTAACTGCAACCTTTCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCTGGGATGGGGAGTGCCAACTTGCGTCTGGGTGGTGATGTGc                                       <  2:300820/293‑1 (MQ=255)                                                                                                                                                                                                                                                                  gTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCAAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCGGGGATGGTGAGTGCCAACTTGCGTCTGGGTGGTGATGTGCaaa                                    >  2:482514/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      cGTGCAGGTAACGCAAACCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCGGGGATGGTGAGTGCCAACTTGCGTCTGGGTGGTGATGTGCAAAGccc                                >  2:786906/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           aGGTAACGCAAACCGGCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTTAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGGGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCGTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCGGGGATGGTGAGTGCCAACTTGCGTCTGGGTGGTGATGTGCAAAGCCCGCAgt                           <  2:103711/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    aaaCCGTCAACGATGCGGCGTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCGGGGATGGTGAGTGCCAACTTGCGTCTGGGTGGTGATGTGCAAAGCCCGCAGTTGTTTGGTc                  <  1:411442/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    ggcgTTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCGGGGATGGTGAGTGCCAACTTGCGTCTGGGTGGTGATGTGCAAAGCCCGCAGTTGTTTGGTCAGCTTCAGGTTACggg  >  1:390023/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        CCGCAATAAGGAGCAAAAAATCAGCATCGGGCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCTGCCCGTGGCGTTTCAGACACTGAATCTGACGGCGGAATTGCGTAACAACCGTGCCGAATTGGGCTGGACCATCCGCCTGACCAATAACGGCCAGTTTGATGGACAGGTGCAGGTGACCGATCCGCAAGGCCGCCGTAATCTTGGTGGCAACGTCAATATCCGTAACTTCAACCTTGCGATGATAAACCCCATCTTTACCCGTGGGGAAAAAGCAGCGGGGATGGTGAGTGCCAACTTGCGTCTGGGTGGTGATGTGCAAAGCCCGCAGTTGTTTGGTCAGCTTCAGGTTACGGG  >  NC_000913/4446187‑4446777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑