Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,321,603 T→C I32V (ATC→GTC)  phnG ← carbon‑phosphorus lyase core complex subunit PhnG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,321,6030TC98.2% 182.3 / NA 56I32V (ATC→GTC) phnGcarbon‑phosphorus lyase core complex subunit PhnG
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (28/27);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGATGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGC  >  NC_000913/4321307‑4321886
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                           
gCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGNCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGACGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTAGGCGGTGACGtt                                                                                                                                                                                                                                                                                          >  2:217735/1‑300 (MQ=255)
     cGGCAATGCGGGCCAGACGGTCAGCGTCCAGCTGGGCAATAAGGGTTTCTGATAGGTTTTGAAAGTGGCGCGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCATGCACACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAgcg                                                                                                                                                                                                                                                                                     <  2:400956/300‑1 (MQ=255)
              gTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGc                                                                                                                                                                                                                                                                            >  2:782511/1‑300 (MQ=255)
              gTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCAGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTAATAGTCGGCGGTGACGTTTAGCGCGTTCAGGc                                                                                                                                                                                                                                                                            >  1:163025/1‑300 (MQ=255)
                 ccATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAACTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGCTTAGCGCGTTCAGGCGtg                                                                                                                                                                                                                                                                         >  1:787873/1‑300 (MQ=255)
                  cATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCAGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCAGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGtgc                                                                                                                                                                                                                                                                        >  1:268261/1‑300 (MQ=255)
                    tACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCNCGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGtgctg                                                                                                                                                                                                                                                                      <  1:606829/300‑1 (MQ=255)
                          cAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGtt                                                                                                                                                                                                                                                                <  2:676943/300‑1 (MQ=255)
                              gTCCAGCTGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGc                                                                                                                                                                                                                                                            <  2:732814/300‑1 (MQ=255)
                                        gCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGGCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCtg                                                                                                                                                                                                                                                  <  1:194849/300‑1 (MQ=255)
                                                             ttttGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAg                                                                                                                                                                                                                             <  1:496203/300‑1 (MQ=255)
                                                                tGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGc                                                                                                                                                                                                                          <  1:379628/300‑1 (MQ=255)
                                                                     gTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCg                                                                                                                                                                                                                     <  1:839788/300‑1 (MQ=255)
                                                                                     aTCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCg                                                                                                                                                                                                     <  2:24589/300‑1 (MQ=255)
                                                                                          cgcgTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATTTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCAt                                                                                                                                                                                                <  1:379793/300‑1 (MQ=255)
                                                                                                 aTCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGGGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGc                                                                                                                                                                                         <  2:528734/300‑1 (MQ=255)
                                                                                                      cgcgcAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCAt                                                                                                                                                                                    <  1:181704/300‑1 (MQ=255)
                                                                                                             cgtgcGGCGTGCTGTTTATCACGCCCCTGCACCTAGCTGTAGCCGAGCGGGCCGTCAGTCAGGCGCACGTAGACGCGGGTCAGAGTGGTGTCGCCGGCAAAAAAACGTTCGCCGGGGCCGCCCATCCGCGCCTGAATCTTTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATATTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAAt                                                                                                                                                                             <  1:248850/296‑1 (MQ=255)
                                                                                                             cGTTCGGCGTGCTGTTTATCACGCCCCTGCACGCAGCTGTAGCCGAGCGTGCCGGCAGGCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAAt                                                                                                                                                                             <  2:266337/300‑1 (MQ=255)
                                                                                                                   gCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCAGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGACGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATc                                                                                                                                                                       >  2:355627/1‑300 (MQ=255)
                                                                                                                                    ccccTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTNTAGCGCGTTCAGGCGTGCTGCCAGTTCCGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAg                                                                                                                                                      >  2:208176/1‑300 (MQ=255)
                                                                                                                                    ccccTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTAATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAg                                                                                                                                                      >  2:671742/1‑300 (MQ=255)
                                                                                                                                    ccccTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTAATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAg                                                                                                                                                      >  1:367947/1‑300 (MQ=255)
                                                                                                                                    ccccTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCACCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGACAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAg                                                                                                                                                      >  2:829732/1‑300 (MQ=255)
                                                                                                                                      ccTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGc                                                                                                                                                    <  1:495931/300‑1 (MQ=255)
                                                                                                                                      ccTGCACCCAGCTGGAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAATGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCATTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCGTGTCGGCGCGCGTCAGGc                                                                                                                                                    <  2:174761/300‑1 (MQ=255)
                                                                                                                                       ctacACCCAGCTGTATCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGTGGGTCAGCGTGGCGTCGCCGGCAAAAGAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGTTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCt                                                                                                                                                   <  2:855489/297‑1 (MQ=255)
                                                                                                                                         gCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCAAGAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGa                                                                                                                                                 >  2:734425/1‑300 (MQ=255)
                                                                                                                                         gCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGAAGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGTGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTAGGCGCGCGTCAGGCTGa                                                                                                                                                 >  2:288447/1‑300 (MQ=255)
                                                                                                                                           aCCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGTGTCAGGCTGACg                                                                                                                                               >  2:680593/1‑300 (MQ=255)
                                                                                                                                           aCCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACg                                                                                                                                               >  1:49555/1‑300 (MQ=255)
                                                                                                                                                                      gtcagGCGCACGGCGACGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCCTAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCg                                                                                                                    >  1:128476/1‑300 (MQ=255)
                                                                                                                                                                      gtcagGCGCACGGCGACGCGGGTCAGCGTGGCGTCGACGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTAATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAATACTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCAGGGCTGCTTTCACCg                                                                                                                    >  2:848361/1‑300 (MQ=255)
                                                                                                                                                                                     acgcgGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAgg                                                                                                     >  1:458280/2‑300 (MQ=255)
                                                                                                                                                                                      cgcgGGTCAGCGTGGCGTCGCCTGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAggg                                                                                                    <  1:815226/300‑1 (MQ=255)
                                                                                                                                                                                      cgcgGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCGCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGNCGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGTTTGGCTGTGCGCCAGCACGGACATNCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAggg                                                                                                    <  2:705990/300‑1 (MQ=255)
                                                                                                                                                                                      cgcgGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGNTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCCCCGTCACGGTGGTTAAggg                                                                                                    >  2:752056/1‑300 (MQ=255)
                                                                                                                                                                                      cgcgGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAggg                                                                                                    <  1:737321/300‑1 (MQ=255)
                                                                                                                                                                                      cgcgGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAggg                                                                                                    <  2:529933/300‑1 (MQ=255)
                                                                                                                                                                                            tcagctntgCGTCGCCGTCAAAAAAACGTTCGCCGGTGCCGCCCAGCCGCGCCTNAATCTGTACCAGTCCAGTTTCAGCTGCGCGGATCACCTCAGAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCATCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTTGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGcgca                                                                                              <  2:707687/292‑1 (MQ=255)
                                                                                                                                                                                            tCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGcgca                                                                                              <  1:793950/300‑1 (MQ=255)
                                                                                                                                                                                               gCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGcgcacgc                                                                                           <  1:819934/300‑1 (MQ=255)
                                                                                                                                                                                                                                               ccTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCAATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAATGGCGACCTATTCGGGATTTCAAGACGCTGACACTCTTTgg                                           >  2:398630/1‑300 (MQ=255)
                                                                                                                                                                                                                                                 tGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGcc                                         <  1:795760/300‑1 (MQ=255)
                                                                                                                                                                                                                                                 tGAATATGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGANATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCANGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGcc                                         <  2:686196/300‑1 (MQ=255)
                                                                                                                                                                                                                                                    aTCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTgg                                      >  2:38778/1‑300 (MQ=255)
                                                                                                                                                                                                                                                         tACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGTCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGcgcg                                 >  1:875177/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                  aGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCNCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCt                        >  2:210757/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                  aGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCNCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGAAGCTGGCATTCGTTGGCCTGGGCGAGGCGGGCGCt                        >  2:210859/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                   gTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTg                       >  1:728294/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                        aGCGGCGCGGATCACCTCATATTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCATTTCAGCCGTTTGTCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGTTCGCGGTGTCTGCGTGCATTCAGTGCTCCATAGTGAATTCAGTCATGTCGGCGCGCGTCAGGCTTACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCcg                  <  2:309929/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     aCCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTgcgg     >  1:58326/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                     aCCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTgcgg     >  2:34003/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                     aCCTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCAATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCAGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCATTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTgcgg     >  2:37380/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                      ccTCATAGTCGGCGGTGACGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTgcggc    >  2:153093/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                        tcgtggtctgtggttaTGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGTCGGTTGGCTGTGCGCCAGCACGGACATCCATGGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTTGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGgcgc  <  2:401860/285‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                           
GCGTGCGGCAATGCGTGCCATACGGTCAGCGTCCAGCGGGGCAATAAGGGTTTCTGATAAGTTTTGAAAGTGGCGAGATTGCTGCATCAGCGCGTCAATCAGCGCGCAGCGTTCGGCGTGCTGTTTATCACGCCCCTGCACCCAGCTGTAGCCGAGCGTGCCGTCAGTCAGGCGCACGGCGGCGCGGGTCAGCGTGGCGTCGCCGGCAAAAAAACGTTCGCCGGTGCCGCCCATCCGCGCCTGAATCTGTACCAGGCCAGTTTCAGCGGCGCGGATCACCTCATAGTCGGCGGTGATGTTTAGCGCGTTCAGGCGTGCTGCCAGTTCAGCCGGTTGGCTGTGCGCCAGCACGGACATCCAGTGCTGGCGGGTCGCGGTATCTGCGTGCATTCAGTGCTCCATAGTGAATTCAATCATGTCGGCGCGCGTCAGGCTGACGGAGTACTCCGCCGGGCTGCTTTCACCGTCACGGTGGTTAAGGGTGCGCACGCACAGCAGCGGCGACATATTCGGGATTTCAAGACGCTGGCACTCTTTGGCCTGGGCGCGGCGGGCGCTGATCCGCGTCTGGCTGCGGCGC  >  NC_000913/4321307‑4321886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑