Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,238,267	IS1 (+) +9 bp	coding (1015‑1023/1521 nt)	mglA ←	D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1978503 =	NA (NA)	84 (0.760)	62/598	0.5	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	2238267 =	0 (0.000)					coding (1023/1521 nt)	mglA	D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit
*	?	NC_000913	= 1979270	NA (NA)	106 (0.950)	71/598	0.2	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 2238275	0 (0.000)					coding (1015/1521 nt)	mglA	D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit

GTAAAATCGTGTTGAGGCCAACGCCCATAATGCGGGCTGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAAT  >  NC_000913/1978972‑1979277                                                                                                                                                                                                                                                                                                           |        gTAAAATCGTGTTGAGGCCAACGCCCATAATGCTGGCTGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTATGTTGCGTACCGGTTTGATAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCAccc        <  2:70685/300‑1 (MQ=2)       tCGTTTTGAGGCCAACGCCCATACTGCGTGCTGGTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAATAAGTTGGCAGCATCACCCaaaacc  <  2:131230/300‑6 (MQ=2)                                                                                                                                                                                                                                                                                                           |        GTAAAATCGTGTTGAGGCCAACGCCCATAATGCGGGCTGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAAT  >  NC_000913/1978972‑1979277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.