Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,136,467	0	A	C	28.8%	85.5 / 13.1	53	E120D (GAA→GAC)	yijE	cystine exporter
Reads supporting (aligned to +/- strand):  ref base A (20/17);  new base C (15/0);  total (36/17)
Fisher's exact test for biased strand distribution p-value = 8.79e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTACTGGTGCCGCAACGTGAAATTGACTGGCAGCCCACCGTGTTCTGGGCGCT  >  NC_000913/4136174‑4136766                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                            aGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTg                                                                                                                                                                                                                                                                                                       >  2:158546/1‑300 (MQ=255)                ttCATGAAGCAAGTCACCAGTTACATCGGTGCCGTCGACTTTACCGCCTTACGCTGCATTGTCTGCGCTCTCGTTTTATTCATCGTCCTTGTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCGCGGTGAACGCCTGCGACGTGGGCAatat                                                                                                                                                                                                                                                                                        <  1:213819/300‑1 (MQ=255)                                  aGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGcc                                                                                                                                                                                                                                                                      >  1:149375/1‑300 (MQ=255)                                      aCATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGNTTCTCGGTGACCGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTNCCGCtt                                                                                                                                                                                                                                                                  >  2:648327/1‑300 (MQ=255)                                                             gCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAg                                                                                                                                                                                                                                           >  2:200024/1‑300 (MQ=255)                                                             gCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAg                                                                                                                                                                                                                                           >  1:616133/1‑300 (MQ=255)                                                               cTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGcc                                                                                                                                                                                                                                         <  1:647760/300‑1 (MQ=255)                                                                ctacgcttcccttctttctctctCGTTTTAGTCGTCGCCCCTTTACGACATGTTCGCGGAATGCGCCCGCCACCGGTGAAATACACCTTACCCGTTTCTCTGTTACCAACCTTCTATCCATTTGTTCTTGCTCAGTGGGCTTTGTTCCGCTTAGGTGCTTGGACGGTTGCACTCCTGAGCTATACCATTCCGTTCTCTGTGGTGATTTTCGCCGCGTGTTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCGGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCg                                                                                                                                                                                                                                        <  2:219404/281‑1 (MQ=255)                                                                  aCGCTGCATTTTCTGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTg                                                                                                                                                                                                                                      <  1:760124/300‑1 (MQ=255)                                                                  aCGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTg                                                                                                                                                                                                                                      >  1:326497/1‑300 (MQ=255)                                                                    gCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTGCTCGGTGAACGCCTGCGACGTGGGCAANATTTCGAGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGc                                                                                                                                                                                                                                    >  2:564610/1‑300 (MQ=255)                                                                                                     tCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGc                                                                                                                                                                                                   <  1:61575/300‑1 (MQ=255)                                                                                                         tttATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGc                                                                                                                                                                                               >  1:31275/1‑300 (MQ=255)                                                                                                                               cgcCCGACACCGTTTAAATACATCTTAGCCATTGCCCTGTTACAAACCTGCTGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATTCCGTTCTTGGTGGTGATTTTCGCCGCGTTGTTTCTCGGGGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTgg                                                                                                                                                                         <  2:607135/300‑1 (MQ=255)                                                                                                                                          gTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTGCGACGTGGGCAATATTTAGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTACGGCGTCAGTTGGGGGGCGAgcgc                                                                                                                                                              >  2:751570/1‑300 (MQ=255)                                                                                                                                          gTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAgcgc                                                                                                                                                              >  1:783525/1‑300 (MQ=255)                                                                                                                                                   cacCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGGACGCCTTCGCCGTGGGCAATGTTTTGCGATTCTGATTGTCGATTTCGGCTTATTTTTGGTGTTGTAGCCGTGGCAACTCGATTTCTCTTAGATGAAAAGTGCAATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGc                                                                                                                                                     >  2:252798/1‑300 (MQ=255)                                                                                                                                                   cacCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTGCGCCGTGGGAAATATTTCGCGATTCTGATTGCAGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGc                                                                                                                                                     >  2:254850/1‑300 (MQ=255)                                                                                                                                                     ccTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTNGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTa                                                                                                                                                   >  1:549812/1‑300 (MQ=255)                                                                                                                                                                     gTTACAAACCTGCGGGATGGTTGGTCTGGCTCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCg                                                                                                                                   >  1:702784/1‑300 (MQ=255)                                                                                                                                                                          aaatcgacGGGATAGTTGGTGTGGCGCAGGGTGCGGTGGTCAGCGGAGGGGTGGGGAAGGTGTTAATCCTGAGCGATACCATCCCGTTCTGTGTGGTGGTTTTCTCCCCTTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATGGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATc                                                                                                                              <  2:669255/293‑1 (MQ=255)                                                                                                                                                                          aaaCCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATc                                                                                                                              <  1:368328/300‑1 (MQ=255)                                                                                                                                                                                           ggtCTGGCGCATCGGTCGTTGGTCATCGTATTTGCGTTGAGGGTGGTGATCCTGAACTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTATTTTTTGGTGAACGCCTTCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTTGCAACTCGATTTCTCTTCGATGAAAAGTGTCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTg                                                                                                             <  2:377718/300‑1 (MQ=255)                                                                                                                                                                                           ggtCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTg                                                                                                             <  1:662779/300‑1 (MQ=255)                                                                                                                                                                                            gtCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACTCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGt                                                                                                            >  1:662768/1‑300 (MQ=255)                                                                                                                                                                                              cTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTGCGACGTGGGCAATATTTAGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCg                                                                                                          >  2:76142/1‑300 (MQ=255)                                                                                                                                                                                                      gTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATc                                                                                                  >  1:700316/1‑300 (MQ=255)                                                                                                                                                                                                               ggTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTTCTAATTGGGCAATATTTGGCGGTTCTGGTTGCCGCTTTCGGCTTATTTTTGGTGTTGTAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTCTGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGAt                                                                                         >  2:307763/1‑300 (MQ=255)                                                                                                                                                                                                                             cGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTACGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTNATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGgcgc                                                                           >  2:576782/1‑300 (MQ=255)                                                                                                                                                                                                                                   aGGTGGCGATCCTGAGCTATACCATGCCGTTNTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGGTCGCCGGCTAAGTGAGAAAGATTTAGGGATTCTGATTGCGGCTTTCGGATTATTTTTNGTGTTGAAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTAGTTAACATCCTGGCAGATGCTGTATGCGGTGCTGGtga                                                                     >  2:564156/1‑300 (MQ=255)                                                                                                                                                                                                                                           aTCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTTCTCCTTAAGCAATATTTAGCGATTCTGATTGCCGCTTTCGGTTTATTTTTGGTGTTGCAGCAGTGGCAACTCGATTTCTATTCGATGAAAAGTGCCATGCTGGCAATCCTCTACGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAATATCCTGGCAGATGCTGTATGTGGTGCTGGTGGTGAGtgtg                                                             >  2:417395/1‑300 (MQ=255)                                                                                                                                                                                                                                            tCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTgg                                                            <  1:762705/300‑1 (MQ=255)                                                                                                                                                                                                                                               ggAGCGATACCATGCCGGTCTGGTTGTTGATTTTCACCTCGTTGTTTCGCGGTGAACGCCTGCGACGTGGGCAAGATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGNTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCNGTCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGTTGGTGATGAGTGTGGTCg                                                         <  2:623855/299‑1 (MQ=255)                                                                                                                                                                                                                                                gcgCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGc                                                        >  1:664143/3‑300 (MQ=255)                                                                                                                                                                                                                                                gAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGGCCGCCTTCTCCTTGGGAAATGTGTAGAGGTTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGTTGGCAATCCTCTCAGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCACGTCATTCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGc                                                        >  2:413080/1‑300 (MQ=255)                                                                                                                                                                                                                                                gAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGGCCGCCTTCTCCTTAGGCAATATTTAGAGGTTCTGATTGCAGCTTTAGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTACGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCTCTGGTGATGCGTGTGGTCGc                                                        >  2:395199/1‑300 (MQ=255)                                                                                                                                                                                                                                                gAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTTCTCATTAGGCAATATTTCGCGATTCTGATTGCCGCTTTTGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTACGGCGTCAGTTGGGGGGCGAGCGAGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACCTCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGc                                                        >  2:121379/1‑300 (MQ=255)                                                                                                                                                                                                                                                gAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTGCGCAGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGGTGAGTGTGGTCGc                                                        >  2:467414/1‑300 (MQ=255)                                                                                                                                                                                                                                                gAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGCCTTGGACAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCAATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGc                                                        >  2:278044/1‑300 (MQ=255)                                                                                                                                                                                                                                                gAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGc                                                        >  1:120024/1‑300 (MQ=255)                                                                                                                                                                                                                                                gAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGACGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGc                                                        >  1:122981/1‑300 (MQ=255)                                                                                                                                                                                                                                                gAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTACGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGc                                                        >  1:4588/1‑300 (MQ=255)                                                                                                                                                                                                                                                gAGCTATACCATGCCGTTCTGGGTGGTGATTNTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGCCGTGGGCAATATTTAGCGATTCTGATTGCCGCTTTCGGATTATTTTTGGTGTTGCAGCCGNGGCAACTCGATTTCTCTTCGATGAAAAGTGCAATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGc                                                        >  2:551849/1‑300 (MQ=255)                                                                                                                                                                                                                                                     ataCCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTTCTCCTTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTAc                                                   >  2:744295/1‑300 (MQ=255)                                                                                                                                                                                                                                                        ccATGCCGTTCTGGGTGTTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTACTgg                                                <  1:375444/300‑1 (MQ=255)                                                                                                                                                                                                                                                        ccATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTACTgg                                                <  1:279705/300‑1 (MQ=255)                                                                                                                                                                                                                                                                gtCTGGTTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGTTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCTGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTACTGGTGCCGCaa                                        <  1:203775/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                     gcgTTGTTTCTCGGTGGCCGCCTTCTCATTGGGCAATGTTTCGAGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTATTCGATGAAAAGTGCCATGCNGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTGTGTATGCCNGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATCAGTGTGGTCGCTTTACTGGTGCCGCAACGTGAAATTGACTGGTAGccc                   >  2:629110/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     gcgTTGTTTCTCGGTGACCGCCTTCTCCGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCNGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCNGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATCAGTGTGGTCGCTTTACTGGTGCCGCAACGTGAAATTGACTGGCAGccc                   >  2:628901/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          gttTCTCGGTGACCGCCTGCGACGTGGGCAATATTTAGAGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTACGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCCGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTGTACTGGTGCCGCAACGTGAAATTGACTGGCAGCCCACCgt              >  2:667226/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                              ctcGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGTTGATGAGTGTGGTCGCTTTACTGGTGCCGCAACGTGAAATTGACTGGCAGCCCACCGTGTTc          <  1:659926/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  gTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTACTGGTGCCGCAACGTGAAATTGACTGGCAGCCCACCGTGTTCTggg      >  2:159224/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   tGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTACTGGTGCCGCAACGTGAAATTGACTGGCAGCCCACCGTGTTCTGGgc     <  1:159244/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      aCGCATGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTACTGGTGCCGCAACGTGAAATTGACTGGCAGCCCACCGTGTTCTGGGCGCt  >  2:101102/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                            AGTTATAGCTGGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTACTGGTGCCGCAACGTGAAATTGACTGGCAGCCCACCGTGTTCTGGGCGCT  >  NC_000913/4136174‑4136766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑