Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,159,413	0	C	T	27.7%	81.2 / 12.4	47	A343V (GCG→GTG)	mdtC	multidrug efflux pump RND permease subunit MdtC
Reads supporting (aligned to +/- strand):  ref base C (12/22);  new base T (13/0);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 4.05e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTG  >  NC_000913/2159143‑2159711                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                            cgGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTcc                                                                                                                                                                                                                                                                               <  1:70375/300‑1 (MQ=255)   gTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCCGGTGCTTTT‑GGTGGTGTTTTTATTCCTg                                                                                                                                                                                                                                                                             >  2:48238/1‑300 (MQ=255)                aTGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTcgcg                                                                                                                                                                                                                                                                >  2:495096/1‑300 (MQ=255)                           gTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTNCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTAGGTGG‑CGCTGGTGGTTCT‑GGTGGGGTTTTTGTTGCTGCGCTCGGGTCGCGCCACTattatt                                                                                                                                                                                                                                                     >  2:172857/1‑300 (MQ=255)                            tGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGANAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTc                                                                                                                                                                                                                                                    <  1:517462/300‑1 (MQ=255)                            tGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTc                                                                                                                                                                                                                                                    <  1:150377/300‑1 (MQ=255)                            agacagACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTc                                                                                                                                                                                                                                                    <  1:690785/295‑1 (MQ=255)                             gACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAANTACCGGAGTTGCAGGAAACCATTCCGNCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGATGGTGGTTTT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTcc                                                                                                                                                                                                                                                   >  2:524892/1‑300 (MQ=255)                                  aCTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCNCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTcctgtat                                                                                                                                                                                                                                              >  2:678122/1‑295 (MQ=255)                                     cAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGNTGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTAGGTGG‑TACTGGTGATTNT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCGCTATTATTCCCGCCGttt                                                                                                                                                                                                                                           >  2:732882/1‑300 (MQ=255)                                       gTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGCTGGTGGTTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCAGTTTCg                                                                                                                                                                                                                                         >  2:739110/1‑300 (MQ=255)                                       gTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGATGGTGCTTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCACGCCGtttag                                                                                                                                                                                                                                         >  2:381723/1‑298 (MQ=255)                                       gTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTAGGTGG‑TGATGGTGGTTCT‑GGGGGTGGGGGTGTTCCTGCGTTCGGGTAGTGCCAATATTATTCCCGCCGTTTCg                                                                                                                                                                                                                                         >  2:350431/1‑300 (MQ=255)                                             gATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TAGTGGTGTTATT‑GGTGGTGGTGTTATTCTTGCGCTCGGGTCGCGCCACTATTATTCCCGCGGTTTAGGTGCCg                                                                                                                                                                                                                                   >  2:363843/1‑300 (MQ=255)                                                gtgCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGGTGGTGCTTTT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGACACTATTATTCCCGAAGTTTAGGTGCAGGtt                                                                                                                                                                                                                                >  2:93917/1‑300 (MQ=255)                                                                      aCGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGTTGGTGGTTTT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCNCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTgcgg                                                                                                                                                                                                          >  2:574791/1‑300 (MQ=255)                                                                               cGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTAcc                                                                                                                                                                                                 <  1:160022/300‑1 (MQ=255)                                                                                    aTTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCAACTATTATTCCNGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTANCTGTGc                                                                                                                                                                                            >  2:640012/1‑300 (MQ=255)                                                                                                        aCTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATGGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCt                                                                                                                                                                        <  1:312209/300‑1 (MQ=255)                                                                                                                      atatTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGGTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGTGCCGCTATTATTCCCGCAGTTTTGGTGCCGGTTTCGCTGATTGGTACGTTTGTGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGgcgt                                                                                                                                                          >  2:86822/1‑299 (MQ=255)                                                                                                                      atatTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGgcgc                                                                                                                                                          >  1:176153/1‑300 (MQ=255)                                                                                                                                   ggTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTAc                                                                                                                                             <  1:475533/300‑1 (MQ=255)                                                                                                                                        aCAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGGTAGTGTTTTT‑GGTGGGGTGTGTATTAATGTGGTTGGGTAGCGACAATATTGTTCCCGCAGGGTAGGTGCAGGTTTCGATGATTGGTAAGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTACTGGTGCTCACCATCGCTACTGGtt                                                                                                                                        >  2:264547/1‑300 (MQ=255)                                                                                                                                        aCAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGGTGGT‑AGTCTTAGGGGGGGGGTGATTAATGTGGTCGGGTAGCGCCAATATTATTCCAGAAGTTTAGGTGCAGGTTTAGATGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCCTTCGTTAATGGCGCTCCCCATCGCTACTGGtt                                                                                                                                        >  2:266277/1‑300 (MQ=255)                                                                                                                                                      aaaTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGAc                                                                                                                          <  1:210004/300‑1 (MQ=255)                                                                                                                                                        aTTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGc                                                                                                                        <  1:682674/300‑1 (MQ=255)                                                                                                                                                                  ttGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCgtggtg                                                                                                              <  1:365173/300‑1 (MQ=255)                                                                                                                                                                    gCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCCTTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TCCCCGTACCCCT‑GACAGTGTTTTTCTTCCTGCGCTTGGGTCGCGCCACTCTTATTCCCGNCGTTTCGGTGCCGGTTTAGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCACTAACCTTTCGTTACTTGTGTCCACCATCGCTACTGGTTTCGTGGTGGACGACGccctcttgttgct                                                                                                            >  2:178899/1‑289 (MQ=255)                                                                                                                                                                        gAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGaa                                                                                                        <  1:48340/300‑1 (MQ=255)                                                                                                                                                                                        cgATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGTCAC‑CGTGCTTCT‑AATGGTGTTTTTATTCCTGCGCTCGGGTCGTGCCGCTATTATTCCCGCCGTTTCGGTGCCGGTTTCGNTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATc                                                                                        >  2:178087/1‑300 (MQ=255)                                                                                                                                                                                                         gCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGGTCAC‑GGTGCCTCT‑GGCGGGGGGGTGAGTAATGAGATCGGGTAGCGCAAATATTATTCCCGAAGTTTAGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTATCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCATGTCATCTGGAAGCGGAAATGaaa                                                                       >  2:114688/1‑300 (MQ=255)                                                                                                                                                                                                         gCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTAATTCT‑AGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGTCACTATTATTCCCGCAGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTAGAAACGGGAATGaaa                                                                       >  2:235701/1‑300 (MQ=255)                                                                                                                                                                                                              ggATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGGTGGTGTTTTT‑GGTGGTGGTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCAGAAGTTTAGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTAATGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATAAAACCGtt                                                                  >  2:349271/1‑300 (MQ=255)                                                                                                                                                                                                                            ccaTTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTAGTGGTTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGc                                                    >  2:475043/1‑300 (MQ=255)                                                                                                                                                                                                                                 cgcgCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GTTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGt                                               <  1:180988/300‑1 (MQ=255)                                                                                                                                                                                                                                       tCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑TGGTGGTGGTTTT‑GGTGGTGTTTTTATTCGTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTcgc                                         >  2:472756/1‑300 (MQ=255)                                                                                                                                                                                                                                        cGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGATGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTcgcg                                        >  2:430199/1‑300 (MQ=255)                                                                                                                                                                                                                                                       aGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GTTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACgg                         <  1:614669/300‑1 (MQ=255)                                                                                                                                                                                                                                                       aGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACgg                         <  1:477860/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  aTTATCTCGGTGG‑CGCTTGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATg              <  1:206545/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  aTTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGGCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATg              <  1:721941/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    tATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAg            <  1:134572/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    tATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGCCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCTGCGATGTACCTGTGCGGATTCAGTCTCAATAACCCTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAg            <  1:185813/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      tctcGTTGG‑CGCTGGTGATTCT‑GTTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTc          <  1:236865/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           gTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCa     <  1:434395/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            tGG‑CGCTGTTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCAc    <  1:392416/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            tGG‑CGCTGGTGATTCT‑GTTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCAc    <  1:412581/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              g‑cgcTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTCCGGTGCTGTCGATGAGTCTGTCCCtg  >  2:423432/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                            CGGTTCGTCTGGGCGATGTGGCGACGGTGACCGACTCAGTGCAGGATGTGCGCAACGCCGGGATGACCAACGCCAAACCGGCTATTTTACTGATGATCCGCAAACTGCCGGAAGCCAATATTATCCAGACGGTTGACAGCATCCGGGCAAAATTACCGGAGTTGCAGGAAACCATTCCGGCGGCGATTGATCTGCAAATTGCCCAGGATCGCTCCCCCACCATTCGCGCCTCGCTGGAAGAAGTCGAGCAAACGCTGATTATCTCGGTGG‑CGCTGGTGATTCT‑GGTGGTGTTTTTATTCCTGCGCTCGGGTCGCGCCACTATTATTCCCGCCGTTTCGGTGCCGGTTTCGCTGATTGGTACGTTTGCGGCGATGTACCTGTGCGGATTCAGTCTCAATAACCTTTCGTTAATGGCGCTCACCATCGCTACTGGTTTCGTGGTGGATGACGCCATCGTGGTGCTGGAAAACATTGCACGTCATCTGGAAGCGGGAATGAAACCGTTGCAAGCCGCACTGCAAGGTACTCGCGAAGTCGGTTTTACGGTGCTGTCGATGAGTCTGTCACTG  >  NC_000913/2159143‑2159711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑