Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,996,049 T→A L156* (TTG→TAG)  yigA → DUF484 domain‑containing protein YigA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,996,0490TA98.6% 244.7 / ‑6.4 70L156* (TTG→TAG) yigADUF484 domain‑containing protein YigA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (37/32);  minor base C (1/0);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTTGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTACACACCGATGTAGAACGCTACCTACGTTATCTGAGCGTGGAGCGC  >  NC_000913/3995753‑3996343
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      
caTGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAggg                                                                                                                                                                                                                                                                                                     <  2:441490/300‑1 (MQ=255)
  tGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGc                                                                                                                                                                                                                                                                                                   <  1:8330/300‑1 (MQ=255)
    gCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAg                                                                                                                                                                                                                                                                                                 >  2:406754/1‑300 (MQ=255)
                 aTCATATTCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATc                                                                                                                                                                                                                                                                                    >  1:649287/1‑300 (MQ=255)
                  tCATATTCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCt                                                                                                                                                                                                                                                                                   <  1:306231/300‑1 (MQ=255)
                       ttCATGTTCTGGAAGAGAGCATGGCGCTGTTGATGGAACAGGCTATCGCCAACTAAGGCCTGTTTTATCGCCTACTCTACTTGCAGCGCAATCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTTGCAGGTGCGAGTCTGCGCCTGTTTCCTGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCGTCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGc                                                                                                                                                                                                                                                                              <  2:330579/300‑1 (MQ=255)
                       ttCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGc                                                                                                                                                                                                                                                                              <  1:383313/300‑1 (MQ=255)
                       ttCATGNTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGNAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGc                                                                                                                                                                                                                                                                              <  2:724473/300‑1 (MQ=255)
                            gTTCTGTAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCtt                                                                                                                                                                                                                                                                         <  1:320921/300‑1 (MQ=255)
                            gTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGTGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTCGGGGCAGGAACAGCACTATCTTAGGCCGCtt                                                                                                                                                                                                                                                                         >  2:523839/1‑300 (MQ=255)
                                       gaACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCaga                                                                                                                                                                                                                                                              >  2:445896/1‑300 (MQ=255)
                                       ctgcgagatgtgtataagagacagggCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCaga                                                                                                                                                                                                                                                              >  1:720205/25‑300 (MQ=255)
                                         aCATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGTGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAgc                                                                                                                                                                                                                                                            <  1:95767/300‑1 (MQ=255)
                                         aCATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAgc                                                                                                                                                                                                                                                            <  2:440486/300‑1 (MQ=255)
                                                  tGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCTTACTCTACCTGCAGCGCAGTCTCACCGCCACCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGGGCGAGTCTGCGCCTGTTTCCGAATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCCGCATATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCtggtgg                                                                                                                                                                                                                                                   <  2:97881/300‑1 (MQ=255)
                                                   gTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTggtggt                                                                                                                                                                                                                                                  <  1:279895/300‑1 (MQ=255)
                                                    ttGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCGGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGgtggtg                                                                                                                                                                                                                                                 <  1:131247/300‑1 (MQ=255)
                                                          gAACAGNCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCNGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCg                                                                                                                                                                                                                                           <  2:717177/300‑1 (MQ=255)
                                                          gAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGTGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCg                                                                                                                                                                                                                                           >  2:258860/1‑300 (MQ=255)
                                                              aGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAg                                                                                                                                                                                                                                       <  1:882135/300‑1 (MQ=255)
                                                               ggCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGc                                                                                                                                                                                                                                      >  2:298142/1‑300 (MQ=255)
                                                                         aaCGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTg                                                                                                                                                                                                                            >  1:28250/1‑300 (MQ=255)
                                                                                    gTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGc                                                                                                                                                                                                                 >  2:828763/1‑300 (MQ=255)
                                                                                    gTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGc                                                                                                                                                                                                                 >  1:777137/1‑300 (MQ=255)
                                                                                                aCTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGCGGGATCGGTGGCGATGTCGATGCt                                                                                                                                                                                                     >  2:321318/1‑300 (MQ=255)
                                                                                                 ctctACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTg                                                                                                                                                                                                    >  1:570655/1‑300 (MQ=255)
                                                                                                       cTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGc                                                                                                                                                                                              <  1:722953/300‑1 (MQ=255)
                                                                                                            gcgcAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGc                                                                                                                                                                                         >  2:191231/1‑300 (MQ=255)
                                                                                                                 gTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGAtt                                                                                                                                                                                    >  1:759858/1‑300 (MQ=255)
                                                                                                                 gTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGAtt                                                                                                                                                                                    >  2:253000/1‑300 (MQ=255)
                                                                                                                         gccgccAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTc                                                                                                                                                                            >  1:296546/1‑300 (MQ=255)
                                                                                                                                    tctcGANGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCNGGTGCGAGTCTGCGCCTGTTTCCTGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTAc                                                                                                                                                                 <  2:715880/300‑1 (MQ=255)
                                                                                                                                       cgacgaTATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCTGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAg                                                                                                                                                              <  1:873996/300‑1 (MQ=255)
                                                                                                                                         gcgaTATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGCTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGACCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACAAGTc                                                                                                                                                            >  2:284638/2‑300 (MQ=255)
                                                                                                                                         acgaTATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTc                                                                                                                                                            >  1:543482/1‑300 (MQ=255)
                                                                                                                                           gaTATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTcgc                                                                                                                                                          >  1:860313/1‑300 (MQ=255)
                                                                                                                                                       cgcTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCAc                                                                                                                                              <  1:693399/300‑1 (MQ=255)
                                                                                                                                                                         cgcgATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGa                                                                                                                            >  1:318448/1‑300 (MQ=255)
                                                                                                                                                                             aTCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGc                                                                                                                        >  1:476183/1‑300 (MQ=255)
                                                                                                                                                                                  ggCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCNGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGNAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTa                                                                                                                   >  2:687226/1‑300 (MQ=255)
                                                                                                                                                                                           ggTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGaa                                                                                                          <  1:823772/300‑1 (MQ=255)
                                                                                                                                                                                            gTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGaaa                                                                                                         >  1:541259/1‑300 (MQ=255)
                                                                                                                                                                                            gTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGaaa                                                                                                         >  2:413131/1‑300 (MQ=255)
                                                                                                                                                                                             tGCGAGTCTGCGCCTGTTTCCTGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGTGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAAt                                                                                                        <  2:457581/300‑1 (MQ=255)
                                                                                                                                                                                                  gTCTGCGTCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGAACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAATCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTgcgc                                                                                                   <  2:273684/300‑1 (MQ=255)
                                                                                                                                                                                                       cgcCTGTTTCCGGATCGCTGGCGCTTAGGTACGCCGTCGAACCACACTCACCTGGCATTAAGCCGTCAGTCTGTCGAACCGCGGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTGAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGAGTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATg                                                                                              <  2:45734/300‑1 (MQ=255)
                                                                                                                                                                                                       cgcCGGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAGCCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGTAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATg                                                                                              <  2:749219/300‑1 (MQ=255)
                                                                                                                                                                                                            gTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGCTCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGCACGCAGTTACTTCATGCAATTGCGCTGATGTTGcc                                                                                         >  1:95055/1‑300 (MQ=255)
                                                                                                                                                                                                            gTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACNATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGcc                                                                                         <  1:640466/300‑1 (MQ=255)
                                                                                                                                                                                                                     tCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGGAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCt                                                                                >  2:341055/1‑300 (MQ=255)
                                                                                                                                                                                                                                 aGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGTCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGAt                                                                    <  2:856509/300‑1 (MQ=255)
                                                                                                                                                                                                                                 aGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGAt                                                                    <  1:362691/300‑1 (MQ=255)
                                                                                                                                                                                                                                                   cacTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGNGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAANTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGAt                                                  >  2:799340/1‑300 (MQ=255)
                                                                                                                                                                                                                                                       cATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTCCTTCATGAAATTGCGCTGCTGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTac                                              >  2:676625/1‑300 (MQ=255)
                                                                                                                                                                                                                                                       cATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTac                                              >  2:830552/1‑300 (MQ=255)
                                                                                                                                                                                                                                                       cATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGANGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTac                                              >  2:752690/1‑300 (MQ=255)
                                                                                                                                                                                                                                                             gCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTACACACCg                                        >  2:392669/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                 tAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTACACACCGATGt                                    <  1:544347/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                     ccGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGNTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTACACACCGATGTAGaa                                <  1:626614/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                     ccGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTACACACCGATGTAGaa                                >  1:544252/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                      cGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTACACACCGATGTAGAAc                               <  1:779654/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                             cTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTACACACCGATGTAGAACGctacct                        >  2:37456/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                             cTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTACACACCGATGTAGAACGctacct                        >  1:679280/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                tCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGAGTTACACACCGATGGAGAACGCTACCTACg                     <  2:404612/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 cGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGTGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGTTGGCGATGTCGATGCTTGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTACACACCGATGTAGAACGCTACCTACGt                    <  1:282822/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  gAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTACACACCGATGTAGAACGCTACCTACGtt                   <  1:183333/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       gCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTACACACCGATGTAGAACGCTACCTACGTTATCTg              >  2:456731/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                   gCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGGTCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGCGCTGTTTACCAGCCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTAATGAAATTGCGCTGATGTTGCCGGAGATTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTACACACCGATGTAGAACGCTACCTACGTTATCTGAGCATGGAgcgc  >  2:68098/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                   gCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTCCACACCGATGTAGAACGCTACCTACGTTATCTGAGCGTGGAgcgc  >  1:304174/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                   gCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGACTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTACACACCGATGTAGAACGCTAACTACGTTATCTGAGCGTGGAgtgc  >  1:150860/1‑297 (MQ=255)
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      
CATGGCCCGCGCACGTAATCATATTCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTTGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAACGCGTATGACCGATTTACACACCGATGTAGAACGCTACCTACGTTATCTGAGCGTGGAGCGC  >  NC_000913/3995753‑3996343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑