Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,320,955	C→T	Q305* (CAG→TAG)	atoS →	sensor histidine kinase AtoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,320,955	0	C	T	100.0%	320.7 / NA	89	Q305* (CAG→TAG)	atoS	sensor histidine kinase AtoS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/57);  total (32/57)

CCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATC  >  NC_000913/2320666‑2321249                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                       ccTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGGCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAANCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGNTTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGTTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTc                                                                                                                                                                                                                                                                                              <  2:624857/300‑1 (MQ=255) ccTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCTACTCTGGCACAAAATATTCCCACTCGATTACCACAATTACCCGGTGAAATGGGGCAAATCATTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATTGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTc                                                                                                                                                                                                                                                                                              <  2:208346/300‑1 (MQ=255) ccTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGTTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTc                                                                                                                                                                                                                                                                                              <  1:455784/300‑1 (MQ=255) ccTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGNTTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATNAACGCCATGAACTGGTAGGGTAGCCTTACTc                                                                                                                                                                                                                                                                                              <  2:546932/300‑1 (MQ=255) ccTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTc                                                                                                                                                                                                                                                                                              <  1:217108/300‑1 (MQ=255) ccTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACCGGTAGGGTAGCCTTACTc                                                                                                                                                                                                                                                                                              >  2:162786/1‑300 (MQ=255)  cTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTcc                                                                                                                                                                                                                                                                                             >  1:481507/1‑300 (MQ=255)     ttCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGNCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTANTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGGGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCAta                                                                                                                                                                                                                                                                                          <  2:613189/300‑2 (MQ=255)      tctcCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATNAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCAtgt                                                                                                                                                                                                                                                                                         <  1:557482/300‑1 (MQ=255)      tctcCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCAtgt                                                                                                                                                                                                                                                                                         <  1:361235/300‑1 (MQ=255)           cGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTTAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCATGGTGATGTAACCACCATGAATCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACGCCAtgtgggtc                                                                                                                                                                                                                                                                                    <  2:387390/300‑6 (MQ=255)                     gcgcCAGTATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGANGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGNTAGCCTTACTCCATGTTGTTCTACAATACTc                                                                                                                                                                                                                                                                          <  2:553601/300‑1 (MQ=255)                     gcgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTc                                                                                                                                                                                                                                                                          <  1:383006/300‑1 (MQ=255)                     gcgcCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGANGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGNTAGCCTTACTCCATGTTGTTCGACAATACTc                                                                                                                                                                                                                                                                          <  2:546835/300‑1 (MQ=255)                         cAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAGTTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGTGGACACTTAACGATCTGATTATTGAAAACGCTACCGATGGCGTCATTGCCATTGACCGCCAGGTTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTcctgtctcttatacacatctcgcag                                                                                                                                                                                                                                                                      <  1:125642/300‑24 (MQ=255)                           atatTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAANGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTNAGTTCt                                                                                                                                                                                                                                                                    >  2:642219/1‑300 (MQ=255)                             atTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCCCTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCACGCACTGCGTGAAACGCGGACACTTAACGATCTGANTATTGAAAACGCTGCCGATGGCGTCANTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTAc                                                                                                                                                                                                                                                                  <  2:514482/300‑1 (MQ=255)                             atTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGNCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTAc                                                                                                                                                                                                                                                                  <  1:566689/300‑1 (MQ=255)                             atTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTAc                                                                                                                                                                                                                                                                  <  2:487954/300‑1 (MQ=255)                               tGATATCATCACCGATGGCCTCTCGACTCTTGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGGGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAg                                                                                                                                                                                                                                                                <  1:343935/300‑1 (MQ=255)                               tGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAg                                                                                                                                                                                                                                                                <  1:675523/300‑1 (MQ=255)                                  tatCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGCTTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACGGCGTGAAACGCGGACACTTAACGATCTGATTATTNAAAACGCTGCCGATGGCGTCATTGCCNTTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTcc                                                                                                                                                                                                                                                             <  2:510295/300‑1 (MQ=255)                                       tcaCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATGGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTAc                                                                                                                                                                                                                                                        <  2:746152/300‑1 (MQ=255)                                         aCCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTg                                                                                                                                                                                                                                                      >  1:621583/1‑300 (MQ=255)                                            gATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGAt                                                                                                                                                                                                                                                   <  1:140381/300‑1 (MQ=255)                                            gATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGCTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGAt                                                                                                                                                                                                                                                   <  2:471280/300‑1 (MQ=255)                                                 ccTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCt                                                                                                                                                                                                                                              >  1:70693/1‑300 (MQ=255)                                                  ctctCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTg                                                                                                                                                                                                                                             >  1:45004/1‑300 (MQ=255)                                                      ggctcggagatgtgtataagagacagcGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACNCTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAAc                                                                                                                                                                                                                                         <  1:548505/274‑1 (MQ=255)                                                      cGACTCTGGCACAAGATATTCCCACTCAAGTACTATAATTGCCCGGTGAAATGGTGCAAATCAGTCAGAGTGGTAATAACCTTGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAAc                                                                                                                                                                                                                                         <  2:79355/300‑1 (MQ=255)                                                      cGACTCTGGCACAAAATATTCCCACTCTATTACCACAACTGCCCGTTGAAATGGGGCAAAGCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGTTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAAc                                                                                                                                                                                                                                         <  1:148908/300‑1 (MQ=255)                                                      cGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATTGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAAc                                                                                                                                                                                                                                         <  2:167280/300‑1 (MQ=255)                                                      cGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAAc                                                                                                                                                                                                                                         <  1:149071/300‑1 (MQ=255)                                                                   aaaTATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACa                                                                                                                                                                                                                            >  1:694467/1‑300 (MQ=255)                                                                                cGATTACCACAATTGCCCGGTGAAATGGGGCNAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCANTGCCATTGACCGCCAGGGTGATGTAAGCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAAcctgtctcttatacacatctggcaggg                                                                                                                                                                                                               >  2:538559/1‑274 (MQ=255)                                                                                    tACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCa                                                                                                                                                                                                           >  1:68561/1‑300 (MQ=255)                                                                                                     gAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATAATCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCAACGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGAc                                                                                                                                                                                          >  2:468626/1‑300 (MQ=255)                                                                                                      aaaTGGTGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAGAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGAcc                                                                                                                                                                                         <  2:671171/300‑1 (MQ=255)                                                                                                      aaaTGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAGAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGAcc                                                                                                                                                                                         <  2:669914/300‑1 (MQ=255)                                                                                                               aaaTCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTg                                                                                                                                                                                <  1:304671/300‑1 (MQ=255)                                                                                                                  tcagtcagAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAAc                                                                                                                                                                             <  1:663193/300‑1 (MQ=255)                                                                                                                   cagtcagAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACt                                                                                                                                                                            >  1:503837/1‑300 (MQ=255)                                                                                                                    agtcagAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGTCGTCATTGCCATTGACCGCCAGNGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTc                                                                                                                                                                           <  2:675627/300‑1 (MQ=255)                                                                                                                    agtcagAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGNTGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTc                                                                                                                                                                           <  1:554495/300‑1 (MQ=255)                                                                                                                     gtcagAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCa                                                                                                                                                                          >  1:81285/1‑300 (MQ=255)                                                                                                                        agagTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAgtg                                                                                                                                                                       <  2:328765/300‑1 (MQ=255)                                                                                                                            tgtTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCNCCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCAc                                                                                                                                                                   <  1:567652/300‑1 (MQ=255)                                                                                                                             gtTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACt                                                                                                                                                                  >  2:8460/1‑300 (MQ=255)                                                                                                                              ttAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAANCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTa                                                                                                                                                                 <  2:689519/300‑1 (MQ=255)                                                                                                                                aataaCCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTAcc                                                                                                                                                               <  1:588881/300‑1 (MQ=255)                                                                                                                                          cccAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCNGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTc                                                                                                                                                     >  2:579435/1‑300 (MQ=255)                                                                                                                                          cccAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTc                                                                                                                                                     >  1:638607/1‑300 (MQ=255)                                                                                                                                          cccAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTc                                                                                                                                                     >  2:144332/1‑300 (MQ=255)                                                                                                                                                 aCTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCAGGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAacac                                                                                                                                              <  2:196446/300‑1 (MQ=255)                                                                                                                                                  cTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAAcacg                                                                                                                                             >  2:2633/1‑300 (MQ=255)                                                                                                                                                    gCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAAcacgca                                                                                                                                           >  1:698369/1‑300 (MQ=255)                                                                                                                                                               ggACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGa                                                                                                                                <  1:615294/300‑1 (MQ=255)                                                                                                                                                                  cccTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTNGGGTAGCCTTACTCCATGTTGTTCGANAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAg                                                                                                                             >  2:522092/3‑300 (MQ=255)                                                                                                                                                                  cccTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAg                                                                                                                             >  1:173745/3‑300 (MQ=255)                                                                                                                                                                                                ccGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTg                                                                                               <  1:5644/300‑1 (MQ=255)                                                                                                                                                                                                      gCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCa                                                                                         >  2:350866/1‑300 (MQ=255)                                                                                                                                                                                                               ccgttGACCTCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTAGCACTGGCTATCAACGCCAGGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATGTAACTGCCCGCAAAGAAAccc                                                                                <  2:102017/297‑1 (MQ=255)                                                                                                                                                                                                               ccATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAAccc                                                                                <  1:324752/300‑1 (MQ=255)                                                                                                                                                                                                                      ccGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAgcgcag                                                                         >  2:428683/1‑298 (MQ=255)                                                                                                                                                                                                                         ccAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCAt                                                                      <  1:667026/300‑1 (MQ=255)                                                                                                                                                                                                                            gggTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGgc                                                                   <  1:605709/300‑1 (MQ=255)                                                                                                                                                                                                                                gATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCaa                                                               >  1:283928/1‑300 (MQ=255)                                                                                                                                                                                                                                     aaCCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGa                                                          >  1:266140/1‑300 (MQ=255)                                                                                                                                                                                                                                         accaTGAACCCAGCAGCAGACGTTATCACTGNCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTCCAGTCCAGTACTGGATACGCTGGAACATGNCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGc                                                      >  2:542448/1‑300 (MQ=255)                                                                                                                                                                                                                                         accaTGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGc                                                      <  1:583877/300‑1 (MQ=255)                                                                                                                                                                                                                                                   catcagcatacgctATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCAGGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGTAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTTAAATGATAGGTGCTTTGTTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCacac                                            <  2:259467/287‑1 (MQ=255)                                                                                                                                                                                                                                                   cagcagcagAAGTTGTCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCacac                                            <  1:215281/300‑1 (MQ=255)                                                                                                                                                                                                                                                   cagcagcagAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGANCATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGGGCCGCATGGCGCAAGCAGAACGCCTCGCCacac                                            <  1:531315/300‑1 (MQ=255)                                                                                                                                                                                                                                                   cagcagcagAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCacac                                            <  2:258161/300‑1 (MQ=255)                                                                                                                                                                                                                                                   cagcagcagAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCacac                                            <  1:65264/300‑1 (MQ=255)                                                                                                                                                                                                                                                    agcagcagAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACt                                           <  1:328424/300‑1 (MQ=255)                                                                                                                                                                                                                                                        gccgaaattATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCATTACTCCATGTTGTTCGAAAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACCTGTCACCGAACATGTTGCGCTGGAGATCCGTTTTCCAGGTCGTGACCGCCCCATTGAATTCAGTGTCACTACCAGTTGTATTCATAAAACGCACGATGAAATGATAGGTGCTTTGGTGATCTTCTCTGATTTCCCTGCCCGTAAAGAAAACCATCGCCGCATGGCGCAAGCAGACCGCCTCGCCCCATTGGGt                                       >  2:209151/8‑300 (MQ=255)                                                                                                                                                                                                                                                        gcagAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGt                                       >  1:668236/1‑300 (MQ=255)                                                                                                                                                                                                                                                           gAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAg                                    >  1:692904/1‑300 (MQ=255)                                                                                                                                                                                                                                                             atttctcccTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTNCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCGGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTCTTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCANAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCt                                  >  2:720272/9‑300 (MQ=255)                                                                                                                                                                                                                                                             aGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGNACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTTATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCt                                  <  2:681527/300‑1 (MQ=255)                                                                                                                                                                                                                                                             aGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCt                                  <  2:337803/300‑1 (MQ=255)                                                                                                                                                                                                                                                             aGTTATCACGGGCTATTAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCt                                  <  1:293181/300‑1 (MQ=255)                                                                                                                                                                                                                                                              gTTATCACTGGCTATCAACACCATGAACTGGTAGGTTAGCCTTACTCCAGGTTATTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGTTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCGCACTGGGTGAGCTg                                 <  2:1165/300‑1 (MQ=255)                                                                                                                                                                                                                                                              gTTACCACTGGCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTg                                 <  2:885/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       ggCTATCAACGCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGAtggctggc                        >  1:676080/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 gCCATGAACTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATg              >  1:508529/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         cTGGTAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGCACATGGCACCGCACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAAACCAGCGCCGCATGGCGCCAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGt      >  1:754348/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           ggtagggtagCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTaa    >  1:42974/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             tAGGGTAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGNCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATc  <  1:531336/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                       CCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATCATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCCGGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACGCGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGCCAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCATGAACTGGTAGGGCAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTACTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGTGACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATGATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATC  >  NC_000913/2320666‑2321249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑