Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,136,053 G→A V164M (GTG→ATG)  flgH → flagellar L‑ring protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,136,0530GA98.9% 331.4 / NA 93V164M (GTG→ATG) flgHflagellar L‑ring protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (54/38);  total (54/39)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGAAGATCGTCGACCACGCAATATTGGCGATACGCTGACCATCGTGTTGCAGGAGAACGTCAGCGCCAGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCACGACGGCGGCTCAGGCTGAGCGTATTCGCGAT  >  NC_000913/1135759‑1136345
                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                    
tttGAAGATCGTCGACCACGCAATATTGGCGATACGCTGACCATCGTGTTGCAGGAGAACGTCAGCGCCAGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTgatgggt                                                                                                                                                                                                                                                                                                 <  1:792972/300‑1 (MQ=255)
       aTCGTCNACCACGCGATATTGTCGATACGCTGACCATCGTGTGGCAGTAGAACGNCAGCGCCAGCAAAAGCTCCTCTTCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGGGCCGATTTCGAAGCCTCCGGTGGTAACACGTTCAACGGAGAGGGCGGTGCCAATGCCAGCAATACCTTTAGCGGCATGTTGACGGTGACGGTTGATCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTTAAAAAc                                                                                                                                                                                                                                                                                          <  2:659241/300‑1 (MQ=255)
            cgACCACGCAATATTGGCGATACGCTGACCATCGTGTTGCAGGAGAACGTCAGCGCCAGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGAtt                                                                                                                                                                                                                                                                                     <  1:287688/300‑1 (MQ=255)
                 aCGCAATATTGGCGATACGCTGACCATCGTGTTGCAGGAGAACGTCAGCGCCAGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCAt                                                                                                                                                                                                                                                                                >  2:425024/1‑300 (MQ=255)
                 aCGCAATATTGGCGATACGCTGACCATCGTGTTGCAGGAGAACGTCAGCGCCAGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCAt                                                                                                                                                                                                                                                                                >  1:507704/1‑300 (MQ=255)
                      atatTGGCGATACGCTGACCATCGTGTTGCAGGAGAACGTCAGCGCCAGCAAAANCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCNGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATc                                                                                                                                                                                                                                                                           <  2:746026/300‑1 (MQ=255)
                      atatTGGCGATACGCTGACCATCGTGTTGCAGGAGAACGTCAGCGCCAGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGTTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATc                                                                                                                                                                                                                                                                           <  2:323035/300‑1 (MQ=255)
                      atatTGGCGATACGCTGACCATCGTGTTGCAGGAGAACGTCAGCGCCAGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTCGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATc                                                                                                                                                                                                                                                                           >  1:259879/1‑300 (MQ=255)
                       gatTGGCGATACGCTGACCATCGTGTTGCAGGAGAACGTCAGCGCCAGCAAAGGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCa                                                                                                                                                                                                                                                                          <  2:30127/299‑1 (MQ=255)
                            gCGATACGCTGCCCATCGTGTTGCAGGAGAACGTCAGCGCCAGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCATCAGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCGTTAATCAGGGTa                                                                                                                                                                                                                                                                     >  2:334942/1‑300 (MQ=255)
                                 aCGCTGACCATCGTGTTGCAGGAGAACGTCAGCGCCAGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTACGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGaa                                                                                                                                                                                                                                                                >  2:501172/1‑300 (MQ=255)
                                   gCTGACCATCGTGTTGCAGGAGAACGTCAGCGCCAGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGCAtt                                                                                                                                                                                                                                                              >  2:724459/1‑300 (MQ=255)
                                      gACCATCGTGTTGCAGGAGAACGTCAGCGCCAGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTAt                                                                                                                                                                                                                                                           >  2:364244/1‑300 (MQ=255)
                                                ttGCAGGAGAACGTCAGCGCCAGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGNGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTctct                                                                                                                                                                                                                                                 >  2:174288/1‑300 (MQ=255)
                                                ttGCAGGAGAACGTCAGCGCCAGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTANCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTctct                                                                                                                                                                                                                                                 >  1:580039/1‑300 (MQ=255)
                                                              cAGCGCCAGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCNATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATcc                                                                                                                                                                                                                                   <  1:555649/300‑1 (MQ=255)
                                                              cAGCGCCAGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTATGCCGCGCTATTTGCATGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATcc                                                                                                                                                                                                                                   <  2:424135/300‑1 (MQ=255)
                                                                     aGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACt                                                                                                                                                                                                                            >  2:601913/1‑300 (MQ=255)
                                                                            gCTCCTTTGCGAATGCCAGCCGTGACGGTAAAACTGATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTAGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCg                                                                                                                                                                                                                     <  2:105842/300‑1 (MQ=255)
                                                                            gCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCg                                                                                                                                                                                                                     <  1:270991/300‑1 (MQ=255)
                                                                             ctcctcTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTAAGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTAACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGATGAAAAACAAATTGCAATTCATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCgg                                                                                                                                                                                                                    >  1:385683/1‑300 (MQ=255)
                                                                              tcctcTGCGAATGCCAGCCGTGACGGTAAAGCTAATTTTGTCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGgc                                                                                                                                                                                                                   <  2:715236/300‑1 (MQ=255)
                                                                                  ctGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGgcagca                                                                                                                                                                                                               >  1:210484/1‑300 (MQ=255)
                                                                                      gAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAAtac                                                                                                                                                                                                           <  1:141401/300‑1 (MQ=255)
                                                                                      gAATACCAGCCGCGACGGTAAAACTAATTTTGGCTTTGATACTGTTCCTCACTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCTGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAAtac                                                                                                                                                                                                           <  2:127971/300‑1 (MQ=255)
                                                                                        aTGCCATCCGTGACGTTAAAACTAATTTTTGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGTTGGTAACACGTTCAACGTAAAGGGCTGGGCCAATGCCAGCAATACCTCTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTTGCGTGGTTAATCCACGCACTATCAGCGGCAGCAAtaccg                                                                                                                                                                                                         <  2:375457/300‑1 (MQ=255)
                                                                                         tGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGAAGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAAtaccgt                                                                                                                                                                                                        >  1:409912/1‑300 (MQ=255)
                                                                                           ccAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACNGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCANGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAAtaccgtac                                                                                                                                                                                                      <  2:624830/300‑1 (MQ=255)
                                                                                                ctttaCGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCATGTGCTGTGTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGGAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGTTGACGGTTGACCAGGTACNAGTCAACGGCAACCTGCATTTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACNCACTATCAGCGGCAGCAATACCGTACCGTcc                                                                                                                                                                                                 <  2:572357/296‑2 (MQ=255)
                                                                                                              tAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACNTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCNGCAATACCGTACCGTCTACTCAGGTGGCGGa                                                                                                                                                                                   <  2:562597/300‑1 (MQ=255)
                                                                                                                       cTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCAt                                                                                                                                                                          >  1:485259/1‑300 (MQ=255)
                                                                                                                         ttGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTg                                                                                                                                                                        >  1:42218/1‑300 (MQ=255)
                                                                                                                         ttGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTg                                                                                                                                                                        >  2:265862/1‑300 (MQ=255)
                                                                                                                               ccgtgCCGCGCTATTTTCATTGACTGTTTTGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGTAAAGGGCGGGGCCAATGCCAGCAATACCTTTGGCGGCACGTTGACGTCGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGGTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATAct                                                                                                                                                                  <  2:383158/298‑2 (MQ=255)
                                                                                                                                 gtgCCGCGCTATTTGCAGGTGCTGTTTGTTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGTTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTa                                                                                                                                                                <  2:479454/300‑1 (MQ=255)
                                                                                                                                 gtgCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCNGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGNGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACgca                                                                                                                                                                >  2:741230/1‑298 (MQ=255)
                                                                                                                                 gtgCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCCGGTGGCGGATGCGCGCATTGAATACGTa                                                                                                                                                                >  1:100709/1‑300 (MQ=255)
                                                                                                                                 gtgCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTa                                                                                                                                                                >  1:339267/1‑300 (MQ=255)
                                                                                                                                 gtgCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTa                                                                                                                                                                >  2:410097/1‑300 (MQ=255)
                                                                                                                                  tgCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAg                                                                                                                                                               <  1:610252/300‑1 (MQ=255)
                                                                                                                                           aTTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACNGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCt                                                                                                                                                      >  1:530644/1‑300 (MQ=255)
                                                                                                                                           aTTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATNCCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCt                                                                                                                                                      >  2:595352/1‑300 (MQ=255)
                                                                                                                                                     gCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAANGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGTTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGa                                                                                                                                            <  2:597528/300‑1 (MQ=255)
                                                                                                                                                     gCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGa                                                                                                                                            <  1:373083/300‑1 (MQ=255)
                                                                                                                                                      cTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTNTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGaa                                                                                                                                           >  2:701499/1‑300 (MQ=255)
                                                                                                                                                      cTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGTGCGCATTGAATACGTAGGCAATGGCTACATTAACGaa                                                                                                                                           >  2:99091/1‑300 (MQ=255)
                                                                                                                                                      cTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGaa                                                                                                                                           >  1:418929/1‑300 (MQ=255)
                                                                                                                                                        gTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACTGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTAAATTAACGAAgc                                                                                                                                         >  2:285973/1‑300 (MQ=255)
                                                                                                                                                        gTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAgc                                                                                                                                         >  1:163970/1‑300 (MQ=255)
                                                                                                                                                        gTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAgc                                                                                                                                         >  2:770438/1‑300 (MQ=255)
                                                                                                                                                               aaCGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAat                                                                                                                                  <  1:234614/300‑1 (MQ=255)
                                                                                                                                                                     cGTGCCGATGTCGAAGCCTACGGTGGTAACACGTTCGACGGAAAGGGCGGGGCCAATGCAAGCAATACCTTTAGTGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAAACTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCGGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACAGTCTACTCAGGTGGCGGATGCGCGCATTGAATCCGTAGGCAATGGCTACATTAACGAAGCGCAAAatacggac                                                                                                                            >  2:126767/1‑295 (MQ=255)
                                                                                                                                                                              gTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAg                                                                                                                   >  1:247996/1‑300 (MQ=255)
                                                                                                                                                                                cGAAGCTTCCGGTGGGAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCATGTTGACGTGGACGGTGGACCAGGCACTGGTCAACGGCAACCGGCATGGGATGGGTGAAAAACAAATGGCAATGAATCAGGGTACCGAATTGATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCg                                                                                                                 <  2:613995/300‑1 (MQ=255)
                                                                                                                                                                                           gtggtAACACGTTCAACGGCAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCCGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTATTCCTTa                                                                                                      >  1:710714/1‑300 (MQ=255)
                                                                                                                                                                                              gtAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCTGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAAcc                                                                                                   <  2:135027/300‑1 (MQ=255)
                                                                                                                                                                                              gtAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAAcc                                                                                                   <  1:229374/300‑1 (MQ=255)
                                                                                                                                                                                                   acGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAAAGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTACTTAACCTGTCg                                                                                              >  2:127512/1‑300 (MQ=255)
                                                                                                                                                                                                    cGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGc                                                                                             <  1:700553/300‑1 (MQ=255)
                                                                                                                                                                                                               aaGGGCNGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTNACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTg                                                                                  <  2:650134/300‑1 (MQ=255)
                                                                                                                                                                                                                 gggCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAg                                                                                >  2:465162/1‑300 (MQ=255)
                                                                                                                                                                                                                        gctaaTGCCAGCAATACCTGTAGTTGCACGTTGGCGTTGACGGTTGACCAGGTACGGGGCAAAGACAGCCTGCATGTGATGGGTGAAAAATGGATTGCCATTAATCAGGGGACCGGATTGATTAGCTGCTCGGGCGTGGTTAATCCACGCACGATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCACGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTgtgg                                                                         <  2:427560/297‑1 (MQ=255)
                                                                                                                                                                                                                        gCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTgtgg                                                                         >  2:444478/1‑300 (MQ=255)
                                                                                                                                                                                                                        gCCAATGCCAGCAATACCGTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTgtgg                                                                         <  1:771100/300‑1 (MQ=255)
                                                                                                                                                                                                                           aaTGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGa                                                                      >  2:492872/1‑300 (MQ=255)
                                                                                                                                                                                                                                cAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTaaa                                                                 >  1:692497/1‑300 (MQ=255)
                                                                                                                                                                                                                                 aGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAAt                                                                >  1:232840/1‑300 (MQ=255)
                                                                                                                                                                                                                                  gCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCNACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGNAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAAtt                                                               >  2:738868/1‑300 (MQ=255)
                                                                                                                                                                                                                                  gCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAAtt                                                               <  2:27749/300‑1 (MQ=255)
                                                                                                                                                                                                                                   cAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTAAATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAAttt                                                              >  1:753321/1‑300 (MQ=255)
                                                                                                                                                                                                                                        ccTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTtctctc                                                         >  1:652411/1‑300 (MQ=255)
                                                                                                                                                                                                                                         cTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTAAATTAACGAAACGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTctctct                                                        >  2:89072/1‑300 (MQ=255)
                                                                                                                                                                                                                                                   aCGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAAttc                                              >  1:268494/1‑300 (MQ=255)
                                                                                                                                                                                                                                                    cGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCACACTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAAttct                                             >  1:514135/1‑300 (MQ=255)
                                                                                                                                                                                                                                                     gcgagatgtgtataagagacAGGTACTGGTCAACGGCAACCTGCCTGTGATGGGTGGAAAACAGATTGCCATTAATCAGGGTACCGAATTTCTTCGCTTCTCTGGTGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTAATCAGGTGGCAGATGCGCGCATTGAATACGTAGGCAATGGCTACATTCACGAAGCGCAAAATATGGGCTGGTTGCCGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAAttctt                                            >  2:54536/20‑300 (MQ=255)
                                                                                                                                                                                                                                                      ttGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAAttcttc                                           >  1:241414/1‑300 (MQ=255)
                                                                                                                                                                                                                                                         acgttgacggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGTCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTAc                                        <  2:783492/296‑1 (MQ=255)
                                                                                                                                                                                                                                                         acggtgacggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACTAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTAc                                        <  1:318173/300‑1 (MQ=255)
                                                                                                                                                                                                                                                         acggtgacggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTAc                                        <  1:724621/300‑1 (MQ=255)
                                                                                                                                                                                                                                                            gtgacggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCAGTAATTCTTCTACTgg                                     <  1:670148/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                cggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCac                                 >  1:606130/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                cggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCac                                 >  2:458097/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                cggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTAAATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTAATGGTCac                                 >  1:675124/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                  gtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTACTTAACCTGTCGCCAATGTAAGTGGGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCacga                               >  1:290683/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                      aCCAGGTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTATCTGCATTAATTCTTCTACTGGTCACGAcggc                           >  1:177297/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                           gTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAGAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTATTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCACGACGGCGtctc                      >  2:290077/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                           gTACTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCACGACGGCGGCTc                      <  1:175048/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                             aCTGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCACGACGGCGGCTCAg                    >  1:704776/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                               tGGTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCACGACGGCGGCTCAGGc                  <  1:432286/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 gTCAACGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCACGACGGCGGCTCAGGCTg                >  1:617883/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                    aaCGGCAATCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACTGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACGCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACTAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATGAATTCTTCTACTGGTCACGACGGCGGCTCAGGCTGAGc             <  2:326774/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     aCGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCNCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTNTACTGGTCACGACGGCGGCTCAGGCTGAGCg            <  2:572701/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     aCGGCAACCTGCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCACGACGGCGGCTCAGGCTGAGCg            <  1:421376/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                               gCATGTGATGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAACATGGGCTGGTTGCAGCGTTTCTTCCTTACCCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCCACTGGTCACGACGGCGGCTCAGGCTGAGCGTATTCGCGAt  >  1:30175/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                    
TTTGAAGATCGTCGACCACGCAATATTGGCGATACGCTGACCATCGTGTTGCAGGAGAACGTCAGCGCCAGCAAAAGCTCCTCTGCGAATGCCAGCCGTGACGGTAAAACTAATTTTGGCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCACGACGGCGGCTCAGGCTGAGCGTATTCGCGAT  >  NC_000913/1135759‑1136345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑