Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,396,772	T→C	F236F (TTT→TTC)	amiB →	N‑acetylmuramoyl‑L‑alanine amidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,396,772	0	T	C	100.0%	281.4 / NA	75	F236F (TTT→TTC)	amiB	N‑acetylmuramoyl‑L‑alanine amidase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/48);  total (27/48)

AAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGATTTACAGTTC  >  NC_000913/4396485‑4397066                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                       aaaCGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTa                                                                                                                                                                                                                                                                                            <  2:462157/300‑1 (MQ=255)  aaCGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATTCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCACTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTaa                                                                                                                                                                                                                                                                                           <  2:70977/300‑1 (MQ=255)    cgcgTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAAcc                                                                                                                                                                                                                                                                                         <  2:492431/300‑1 (MQ=255)    cgcgTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAAcc                                                                                                                                                                                                                                                                                         <  1:148800/300‑1 (MQ=255)      cgTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCNCGACGGCTAACACTGGCGATAAAATTNTCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCg                                                                                                                                                                                                                                                                                       >  2:540029/1‑300 (MQ=255)              cGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCAACATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACgggg                                                                                                                                                                                                                                                                               >  1:495185/1‑300 (MQ=255)               gCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGa                                                                                                                                                                                                                                                                              >  2:304707/1‑300 (MQ=255)                   gCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTGCTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTAc                                                                                                                                                                                                                                                                          <  1:462115/300‑1 (MQ=255)                     ggTTGTCGCACCGCGCTTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTTGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACtt                                                                                                                                                                                                                                                                        <  2:132540/300‑1 (MQ=255)                     ggTTGTCGCACCGCGCGTCAGCGAACCTGCGCGCAATCCGTTTAAAACGGAAGGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACtt                                                                                                                                                                                                                                                                        <  1:723959/300‑1 (MQ=255)                          tCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTAtct                                                                                                                                                                                                                                                                   <  1:394303/300‑1 (MQ=255)                                   gcgTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATgg                                                                                                                                                                                                                                                          <  1:757801/300‑1 (MQ=255)                                     gTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATgggg                                                                                                                                                                                                                                                        <  2:685491/300‑1 (MQ=255)                                        aGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGgcgc                                                                                                                                                                                                                                                     <  1:773627/300‑1 (MQ=255)                                           taaCCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTGATCNGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCGAACACTTTCGATAAAATTACCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAACGACGATCCGATGTTCAAAGGCCTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGt                                                                                                                                                                                                                                                  <  2:597021/299‑1 (MQ=255)                                           gAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGt                                                                                                                                                                                                                                                  <  1:156762/300‑1 (MQ=255)                                           gAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCATGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATTGGGCGCAGt                                                                                                                                                                                                                                                  <  1:106081/300‑1 (MQ=255)                                             aCCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGa                                                                                                                                                                                                                                                <  1:449804/300‑1 (MQ=255)                                             aCCGGCGCGCAAGCCGTGTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGGAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACNCTGGCGCTATCGGCCCCGGGGGTACGNGGTAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGa                                                                                                                                                                                                                                                <  2:535035/300‑1 (MQ=255)                                                    cgcAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCa                                                                                                                                                                                                                                         <  1:142765/300‑1 (MQ=255)                                                    cgcAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCAGCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCa                                                                                                                                                                                                                                         <  1:142808/300‑1 (MQ=255)                                                                                   cTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGAAGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGa                                                                                                                                                                                                          >  1:343324/1‑300 (MQ=255)                                                                                          tgttagcggcggTAATACGGTAACGCGGCCTGCAGTGCGCGCTATGTCTAACACTGGCGATAAAATGATCATCGTTATTGATGCAGGACACGGCGGTCAGGACCCTGGCACTATCGGCCCCGGTGGTACGCGGGAGAAAAATTTCACCATCGCCATCGCGCGTAAATTTCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACgt                                                                                                                                                                                                   <  2:776087/289‑2 (MQ=255)                                                                                                catcagTAATACGTTAACGTGTCCGGCAACGCGCGCCACGGCTAACACGAGCGATAAAATTATCATCGTTATTGATGCAGGACACGGCATTCATTACCCTGGCGCTATCCGCTCCGGTGGTACGCGGTAGAAAAATGTCACCATCTCCATCGCGCGTAAATTACTTACTTTGCTCAACGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGACTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGc                                                                                                                                                                                             <  2:121850/297‑1 (MQ=255)                                                                                                   cagTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGACATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCTCAGTGATGTGGCACGTAAGCAAAAGGCCAATTTCCTCGTGTCGATTCACgttggtgcagc                                                                                                                                                                                          >  2:217582/1‑290 (MQ=255)                                                                                                     gTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTGTCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCAc                                                                                                                                                                                        >  2:718894/1‑300 (MQ=255)                                                                                                        aTACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGGTGGGGTGCAGTGATGTGGCACGTAAGCAAAACGCCAATCTCCTCGTGTCGATTCACGCTGATGCCGCACCGa                                                                                                                                                                                     >  2:450988/1‑300 (MQ=255)                                                                                                             ttAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTGCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGc                                                                                                                                                                                <  1:342454/299‑1 (MQ=255)                                                                                                                    gTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGNTGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGGTGGGGCGCAGTGATGTGGAAAGTAAGAAAAAAGAAAATGTCCTAGTGTCGATTAACGCTGATGCCGCACCGAACCGCAGTGCgg                                                                                                                                                                         >  2:185201/1‑299 (MQ=255)                                                                                                                    gTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCAAGTAAGAAAAACGCCAATTTACTAGTGTCGATTCACGCTGATGCAGAACCCAACCGCAGTgtgg                                                                                                                                                                         >  2:254497/1‑297 (MQ=255)                                                                                                                       cggacgtgcactcgACTGCTAACGCTTGCGATAAAATTAGCGTCGCTATTGATGCCGGACAAGGCGGTCAGGATCCTTGCGCTATCGGCCCCGGTGGTACGCGGGATAAAAATATCACCATCTACATCGCGCGCAAATTGCGTACTTTGCTCAATTACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTGTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGTTGATGCCGCACCGAACCGCAGTGCGACTg                                                                                                                                                                      <  2:74861/288‑1 (MQ=255)                                                                                                                                  gcgACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTa                                                                                                                                                           <  1:392347/300‑1 (MQ=255)                                                                                                                                      cGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATggg                                                                                                                                                       <  1:474205/300‑1 (MQ=255)                                                                                                                                       ggCTAACACTTGAGATAAGATTAGCATCGCTATTGATGCCGGACATGGCGGTCAGGACCCTGGCGCGATCGGCCTTGGTGGTACGCGGGAGAAAAACGTCACCATCGCCATCGCGCGTAAATTGCTTATTTTGCTCAATTACGATCCGATGTTCAAAGGTGTTTTAACCCTTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTTTCGATTCACGCTGATGCCGCACCGAACTGCAGTGCGACTGGCGCTTCCGTATGGGt                                                                                                                                                      <  2:712116/300‑1 (MQ=255)                                                                                                                                       ggCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGNGTACTTTGCTCAATGACGATCCGATGNTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGt                                                                                                                                                      >  2:523451/1‑300 (MQ=255)                                                                                                                                       ggCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGt                                                                                                                                                      >  1:744888/1‑300 (MQ=255)                                                                                                                                       ggCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGt                                                                                                                                                      >  2:77310/1‑300 (MQ=255)                                                                                                                                        gCTAACACTTTCAATAAAACTGTCATCGCTATTGATGCCGGACACGGCGATCAGGACCCTGTCACTATCGGCCTCGGTGGGACGCGGGAGAAAAATGTCACCACCGCCATCGCGCGTAAATTACGTACTTTACTCAACGACGATCCGATGGGCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGGGGCACTTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGATTGGCGCTTCCGTATGGGTg                                                                                                                                                     <  2:308979/300‑1 (MQ=255)                                                                                                                                         cTAACACTGGCTATAAAACTATCATCACGATTGATGCAGGACACGTCGGGCAGGGACCTGTCACTATCGGCCCCGTTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGGACTTTGCTCAACGACAATCCGATGTTCAAATGCGTTTCAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTgt                                                                                                                                                    <  2:92716/300‑2 (MQ=255)                                                                                                                                          tAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGAAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTGct                                                                                                                                                   >  2:677569/1‑300 (MQ=255)                                                                                                                                             cacgtgataggaaactATCGTCGCTGTTGATTCCGGACACGGCAGTTAGGACCCTGGCGCCACCGTCCTAGGTGGGACGCGGGAGGAAAATATCACCACCGCCATCGCGCGTGAATTGCGTACTTTGCTCAATGACAATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATCACTTTATCTCGTTGATGGGGCGCAGTGATGGGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGctcgc                                                                                                                                                <  2:109178/285‑3 (MQ=255)                                                                                                                                             cacTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGctctc                                                                                                                                                <  1:203012/300‑1 (MQ=255)                                                                                                                                             cacTAGCGATAAAACTATCATCGCGATGGATACCACACACGGCGGTCAGGACCCTGGCACCACCACCCCCGGGGGTACGAGTGAAAAAAATGTCACCATCGCCATCGCGCGTACATTGCGTACGTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCCTGACGGGGATTACTTTATCTCGGTGATGGTGCGCATTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCACACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGctcgc                                                                                                                                                <  2:109373/300‑3 (MQ=255)                                                                                                                                              acTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGctctct                                                                                                                                               <  1:660390/300‑1 (MQ=255)                                                                                                                                                  gCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAAcc                                                                                                                                           <  1:145054/300‑1 (MQ=255)                                                                                                                                                   cGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCg                                                                                                                                          >  1:249834/1‑300 (MQ=255)                                                                                                                                                               catcGCTGTTGATGCCGGACATTGCGGTCAGGACCCTTGCGCTATCGGCCCAGGNGGTACGCGTGAGAAAAATGTCACCATCCCCATCGCGCGTAAATTTCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACTGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAg                                                                                                                              <  2:578813/300‑1 (MQ=255)                                                                                                                                                                 tcGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTTACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTg                                                                                                                            >  2:458918/1‑300 (MQ=255)                                                                                                                                                                 tcGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTAGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTg                                                                                                                            >  2:759510/1‑300 (MQ=255)                                                                                                                                                                     tatttataccGGACACTGCGGTCAGGACCCTGGCGCTATCGGCCCCGGCAGGACGAGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGCTCCGAGGTTCAAAGGCGTTTTAACCCGGGACGGGGATTACTTTATCGCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAAt                                                                                                                        <  2:140302/292‑1 (MQ=255)                                                                                                                                                                     tATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATNACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGaaac                                                                                                                        <  1:554366/300‑2 (MQ=255)                                                                                                                                                                                      gcggTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGagc                                                                                                       <  1:734732/300‑1 (MQ=255)                                                                                                                                                                                        ggTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATNGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTNTGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGagcag                                                                                                     >  2:748364/1‑300 (MQ=255)                                                                                                                                                                                        ggTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGGTGGGGGGCAGTGGTGTGGAAGGTAGGCAAAACGCCAATTTCCTAGTGTCGATTCACGCTGATGCAGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGACAGCTGGCTGGagcag                                                                                                     >  2:603534/1‑300 (MQ=255)                                                                                                                                                                                        ggTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGagcag                                                                                                     <  1:603156/300‑1 (MQ=255)                                                                                                                                                                                               aCCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACgaga                                                                                              >  1:104459/1‑300 (MQ=255)                                                                                                                                                                                                cccgtgcgcTATCTGCCCCGGTGATACGCGGTAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTGAACCCGTTACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGaa                                                                                             <  2:360278/295‑1 (MQ=255)                                                                                                                                                                                                        cTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTAGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCNGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCgg                                                                                     >  1:553082/1‑300 (MQ=255)                                                                                                                                                                                                             ggCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTAGGNGATGGGGAGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTNATGCCGCACCGAACCGCAGTGCGACTGGCGCTTACGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAgctg                                                                                >  2:632497/1‑300 (MQ=255)                                                                                                                                                                                                                cccGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGctgctg                                                                             <  1:696641/300‑1 (MQ=255)                                                                                                                                                                                                                  cGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCAAGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGATTCCGTATGGGTGCTCTATAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCtggg                                                                           >  2:357898/1‑300 (MQ=255)                                                                                                                                                                                                                    gtggtACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGAGAAGCGATGTGGAAAGTAAGAAAAACGCCAATTTCCTAGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCAGACAGGGAAAGGGCAAGCTGGCTGGGGCAGCACGAGAAACAGTCGGAGCTGTtgggtg                                                                         >  2:161628/1‑300 (MQ=255)                                                                                                                                                                                                                             gggAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTg                                                                <  1:53337/300‑1 (MQ=255)                                                                                                                                                                                                                              ggAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGa                                                               <  1:508549/300‑1 (MQ=255)                                                                                                                                                                                                                                agaAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGGTGGGGGGCAGTGATGTGGAAAGTAGGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGACAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGAtg                                                             >  2:340914/1‑300 (MQ=255)                                                                                                                                                                                                                                                   catcgcGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACagtcagtc                                          <  1:100636/300‑1 (MQ=255)                                                                                                                                                                                                                                                         gcgTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGTTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACTAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGAccc                                    <  1:695196/300‑1 (MQ=255)                                                                                                                                                                                                                                                         gcgTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGTGCGCAGTGATGTGGCACGTAAGCAAGACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGAccc                                    <  1:298442/300‑1 (MQ=255)                                                                                                                                                                                                                                                         gcgTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGAccc                                    <  1:113110/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 tGCGTACTTTGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTaa                            >  1:631976/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  gctgattttGCTCAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAg                           <  2:511899/294‑1 (MQ=255)                                                                                                                                                                                                                                                                    ttACTTTGCTCAACGACGATCCGCTGTTCAGAGGCGTTTTAACCCTTGACGGGGAGGACTTTATCTCGGTGGTGGGGCGCAGTGATGTGGCACGGAAGCAAAACGCCAATTTCCTCGTGTCGATTTACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGcc                         <  2:144037/299‑1 (MQ=255)                                                                                                                                                                                                                                                                              cAATGACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGGTAGGGAGCAGTGGTGTGGCAAGTAAGAAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCt               >  2:611503/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  gACGATCCGATGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGAt           <  1:729281/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                           aTGTTCAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGATTTACAGTTc  >  1:335470/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                       AAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGCGACGGCTAACACTGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCCGGTGGTACGCGGGAGAAAAATGTCACCATCGCCATCGCGCGTAAATTGCGTACTTTGCTCAATGACGATCCGATGTTTAAAGGCGTTTTAACCCGTGACGGGGATTACTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTTCCTCGTGTCGATTCACGCTGATGCCGCACCGAACCGCAGTGCGACTGGCGCTTCCGTATGGGTGCTCTCTAACCGTCGCGCCAACAGTGAAATGGCCAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTGCTGGGTGGGGCGGGTGATGTGCTGGCGAACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGATTTACAGTTC  >  NC_000913/4396485‑4397066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑