Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	395,831	G→A	R153C (CGT→TGT)	ampH ←	peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	395,831	0	G	A	100.0%	217.3 / NA	61	R153C (CGT→TGT)	ampH	peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/28);  total (33/28)

AATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGAT  >  NC_000913/395541‑396122                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                    aaTTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCcgc                                                                                                                                                                                                                                                                                            <  2:482517/300‑1 (MQ=255)           aGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGTCGGCACTGATCCGGTGAGGGGTTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTNCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGc                                                                                                                                                                                                                                                                                  <  1:579537/300‑1 (MQ=255)           aGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGc                                                                                                                                                                                                                                                                                  <  2:343852/300‑1 (MQ=255)              gtgtTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGt                                                                                                                                                                                                                                                                               >  2:113257/1‑300 (MQ=255)                     tGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGGGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACggg                                                                                                                                                                                                                                                                        <  1:457074/300‑1 (MQ=255)                           gACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCACACAACTGGGTATAATACCTGCCTAGCGCATTCTCACA‑TGCCTCTGCCAGCAGGTCGAACGCAAGGTTAGAGCCCTCCGCATGACTACCCGACGCGGCTTTCAGCTTCGCAGTAGAAAGGTATTTACAGCGTTGCTCGCGCGTTGGCCAGACAAACACAGGACAATGTGCCGCGCAAACGGGCTGTTCACGGGGCAggg                                                                                                                                                                                                                                                                  >  2:452859/1‑300 (MQ=255)                           gACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAggg                                                                                                                                                                                                                                                                  >  2:452934/1‑300 (MQ=255)                            aCTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGgc                                                                                                                                                                                                                                                                 <  1:55225/300‑1 (MQ=255)                                gCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATTTGCTCTTCAAACCACTGGGTATAAGGCTTGCAGGACGCATTCGC‑CAGTGCATCTGCAAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTCCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGTAGGGTGCTg                                                                                                                                                                                                                                                             >  2:134580/1‑300 (MQ=255)                                       gcTCAGCAACCATTAAACGGCGGCACTGATCCGGTGATGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATggg                                                                                                                                                                                                                                                      <  2:242666/300‑1 (MQ=255)                                        cTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGt                                                                                                                                                                                                                                                     <  1:113593/300‑1 (MQ=255)                                         tCAGCAACCATTAAACTGCGGCACTGATCCGGTGATGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCGTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACTGGACAGTGTGCCGCGCCACCGTGCTGTTCACGGGGCAGGGCGCTGGTATggggt                                                                                                                                                                                                                                                    <  2:382724/300‑3 (MQ=255)                                                               aCTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGTTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATgg                                                                                                                                                                                                                              <  1:660570/300‑1 (MQ=255)                                                               aCTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATgg                                                                                                                                                                                                                              <  1:661857/300‑1 (MQ=255)                                                               aCTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATgg                                                                                                                                                                                                                              <  2:507176/300‑1 (MQ=255)                                                                  gATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCg                                                                                                                                                                                                                           >  1:486659/1‑300 (MQ=255)                                                                  gATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCg                                                                                                                                                                                                                           >  2:489746/1‑300 (MQ=255)                                                                          gAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTNGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTg                                                                                                                                                                                                                   >  1:565240/1‑300 (MQ=255)                                                                            gggggTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTa                                                                                                                                                                                                                 >  1:688798/1‑300 (MQ=255)                                                                              gggTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAgg                                                                                                                                                                                                               <  1:784787/300‑1 (MQ=255)                                                                               ggTGTAGGTGGTGTCTTTCATCCCCAATGTACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCATGGCGCGGCTTGCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGt                                                                                                                                                                                                              <  2:503072/300‑1 (MQ=255)                                                                               ggTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTNGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCANGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGt                                                                                                                                                                                                              >  2:730865/1‑300 (MQ=255)                                                                               ggTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGGAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGTGCTGGTATGGGTTGCCAGATTGACCAGCGTAGTGGGCGTCCAATTGTAGGt                                                                                                                                                                                                              >  2:278411/1‑300 (MQ=255)                                                                               ggTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGt                                                                                                                                                                                                              <  1:248631/300‑1 (MQ=255)                                                                               ggTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCAATTGTAGGt                                                                                                                                                                                                              >  1:36/1‑300 (MQ=255)                                                                               ggTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCAATGGTAGGt                                                                                                                                                                                                              >  2:676552/1‑300 (MQ=255)                                                                               ggTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGCTTGCCAGATTGACCAGCGTAATGGGCGTCCCATCGTAGGt                                                                                                                                                                                                              >  2:681052/1‑300 (MQ=255)                                                                               ggTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTAAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGCCAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCAATTGTAGGt                                                                                                                                                                                                              >  2:612012/1‑300 (MQ=255)                                                                               ggTGTAGATGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCCTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCAAGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGt                                                                                                                                                                                                              >  2:714597/1‑300 (MQ=255)                                                                                      ttggtgTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTNAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCAcg                                                                                                                                                                                                       <  1:533403/299‑1 (MQ=255)                                                                                             cTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTgcgc                                                                                                                                                                                                >  1:344866/1‑300 (MQ=255)                                                                                             cTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTgcgc                                                                                                                                                                                                >  2:602923/1‑300 (MQ=255)                                                                                                              cGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGNGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTa                                                                                                                                                                               >  1:585155/1‑300 (MQ=255)                                                                                                                ggTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACt                                                                                                                                                                             >  1:654463/1‑300 (MQ=255)                                                                                                                        gCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGAt                                                                                                                                                                     >  1:155638/1‑300 (MQ=255)                                                                                                                              cAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTa                                                                                                                                                               <  1:216960/300‑1 (MQ=255)                                                                                                                                         gTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTc                                                                                                                                                    >  1:434981/1‑300 (MQ=255)                                                                                                                                         gTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCAATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTc                                                                                                                                                    >  1:139364/1‑300 (MQ=255)                                                                                                                                         gTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTACAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGta                                                                                                                                                    >  2:303068/1‑299 (MQ=255)                                                                                                                                                 cTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTc                                                                                                                                            <  1:438263/300‑1 (MQ=255)                                                                                                                                                           gCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTc                                                                                                                                  >  1:658922/1‑300 (MQ=255)                                                                                                                                                                      gTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCACTGGTCGAGCAATTTCACCAGCAtct                                                                                                                        >  1:686490/1‑300 (MQ=255)                                                                                                                                                                            cTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTgg                                                                                                                  <  1:459076/300‑1 (MQ=255)                                                                                                                                                                                    agttCGAACGCAAGGTGAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCTTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACNCCGTACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAACTTCACCAGCATCTCACTGGTCATTAGc                                                                                                          <  2:696570/297‑1 (MQ=255)                                                                                                                                                                                    agGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCTGCTTTCAGCTTCGCCGTAGAAAGGTGTTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGc                                                                                                          <  1:190315/300‑1 (MQ=255)                                                                                                                                                                                     tcggagatgtgtataagagacaggccgccTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCt                                                                                                         <  1:416114/277‑1 (MQ=255)                                                                                                                                                                                     ggTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCTTAGAAAGGTATTTCCAGCGTTACTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACTGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGTTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCt                                                                                                         <  2:112780/300‑1 (MQ=255)                                                                                                                                                                                      gTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGACCAGACAAACACCGGACAATGTGCCGAGCCACCGGGCTGTTCACGGGGCAGGGCGATGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTACAATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTAACGGTACACTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCtt                                                                                                        >  2:108035/1‑300 (MQ=255)                                                                                                                                                                                          aaCGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGGTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTg                                                                                                    <  2:303956/300‑1 (MQ=255)                                                                                                                                                                                                 ggTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAg                                                                                             >  1:749039/1‑300 (MQ=255)                                                                                                                                                                                                         tACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGTTCATTAGCTTGGTGAGGGAAGCAATACGc                                                                                     <  2:765482/300‑1 (MQ=255)                                                                                                                                                                                                          aCGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGcctgtctcttatacacatctcgc                                                                                    <  1:751593/300‑23 (MQ=255)                                                                                                                                                                                                          aCGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCa                                                                                    <  1:51917/300‑1 (MQ=255)                                                                                                                                                                                                            gccgccTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCAATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTAACTGGTCATTAGCtctgtctcttatacacatctagca                                                                                  >  2:412085/1‑277 (MQ=255)                                                                                                                                                                                                                                            cGCCGTATAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACTGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTAcc                                                  <  1:614085/300‑1 (MQ=255)                                                                                                                                                                                                                                                gTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTACGGGCGAACGTTATTACCAGGt                                              >  2:12156/1‑300 (MQ=255)                                                                                                                                                                                                                                                                aGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAAc                              <  1:520139/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      gCTCGCGCGTTGGCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCAACAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGaa                        >  1:717167/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 ggCCAGACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTgg             >  1:604544/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      gACAAACACCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTAcc        >  1:596332/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            cacCGGACAATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCTCTGGTTACCGTCGAt  >  1:29869/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                    AATTGCCGCCAGCGTGTTATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGC‑CAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACCGGACGATGTGCCGCGCCACCGGGCTGTTCACGGGGCAGGGCGCTGGTATGGGTTGCCAGATTGACCAGCGTAATGGGCGTCCCATTGTAGGTTGGCACGCGTGCGCCTGGCGGAGCATATTTACTTAGCGGATCGTTTAACTTCACGGTCCCCTGGTCGAGCAATTTCACCAGCATCTCACTGGTCATTAGCTTGGTGAGGGAAGCAATACGCACGACCGAATCCAGCTGCGGGCGAACGTTATTACCAGGTCGCGTTTCGCCATAACTGCGAAAGACGCGCTGGTTACCGTCGAT  >  NC_000913/395541‑396122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑