Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,823,162	C→T	H255Y (CAC→TAC)	spoT →	bifunctional (p)ppGpp synthase/hydrolase SpoT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,823,162	0	C	T	100.0%	294.4 / NA	82	H255Y (CAC→TAC)	spoT	bifunctional (p)ppGpp synthase/hydrolase SpoT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/51);  total (31/51)

CGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTAC  >  NC_000913/3822877‑3823445                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                            cGTGAAACTCTCGAAGTTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGAc                                                                                                                                                                                                                                                                               <  1:399375/300‑1 (MQ=255) cGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGAc                                                                                                                                                                                                                                                                               <  1:478735/300‑1 (MQ=255) cGTGAAACTCTCGAAATTTATAGCCCGCGGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGAc                                                                                                                                                                                                                                                                               <  1:72618/300‑1 (MQ=255)         tctcGAAATTTATAGCCCGCTGGCGCACCGTTGAGGTATCCACCACATTAAAACTGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGc                                                                                                                                                                                                                                                                       <  2:49343/300‑1 (MQ=255)                            cTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAAt                                                                                                                                                                                                                                                    >  1:10953/1‑300 (MQ=255)                                cgcACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAATTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCTTGATCGTCAATGAtt                                                                                                                                                                                                                                                <  1:660625/300‑1 (MQ=255)                                    ccGTTTAGGTATCCACCACATTAAAACCGAANTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGNTCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGCTTCTGa                                                                                                                                                                                                                                            >  2:568247/1‑300 (MQ=255)                                     cGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGac                                                                                                                                                                                                                                           <  1:391000/300‑1 (MQ=255)                                      gTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTCCGCTTTCCGCGTGATCGTCAATGATTCTGaca                                                                                                                                                                                                                                          >  2:6666/1‑300 (MQ=255)                                      gTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGaca                                                                                                                                                                                                                                          >  1:127314/1‑300 (MQ=255)                                            gTATCCACCACATTAAAACCGAACTCGAAGAGTTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCCTCTTTATTCGATTTACTGCCAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGtt                                                                                                                                                                                                                                    >  1:724799/1‑300 (MQ=255)                                            gTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGANAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGtt                                                                                                                                                                                                                                    >  1:577001/1‑300 (MQ=255)                                               tCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGANATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATc                                                                                                                                                                                                                                 <  1:575912/300‑1 (MQ=255)                                                ccaccaCATTAAAACCGAACTCTAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATcg                                                                                                                                                                                                                                <  1:71229/300‑1 (MQ=255)                                                ccaccaCATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATcg                                                                                                                                                                                                                                <  1:299533/300‑1 (MQ=255)                                                  accacATTAAAACCGAACTCTAAGAACTGGGTTTTTAGGCGCTGTACCCCAACCGTCATCGCGTAATCAAATAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAATAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATcgcg                                                                                                                                                                                                                              <  2:45313/300‑1 (MQ=255)                                                  accacATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAATTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAATAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCTTGATCGTCAATGATTCTGACACCTGTTATcgcg                                                                                                                                                                                                                              <  1:186438/300‑1 (MQ=255)                                                  accacATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATcgcg                                                                                                                                                                                                                              <  1:699113/300‑1 (MQ=255)                                                                   cTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTACTCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTNCCGCGTCAGTGGTCGCGAGAAGCATCNTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGcac                                                                                                                                                                                                             <  2:527301/300‑1 (MQ=255)                                                                     cGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAg                                                                                                                                                                                                           <  1:750210/300‑1 (MQ=255)                                                                     cGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAg                                                                                                                                                                                                           <  2:126900/300‑1 (MQ=255)                                                                      gAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTTGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGc                                                                                                                                                                                                          <  2:760018/300‑1 (MQ=255)                                                                      gAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGCTTTTAATCGATCATGGACATCTAAGCTTTCCGCGTGGTCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATACACAGc                                                                                                                                                                                                          >  2:2628/1‑300 (MQ=255)                                                                       aaGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGcc                                                                                                                                                                                                         <  1:355298/300‑1 (MQ=255)                                                                            cTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTAc                                                                                                                                                                                                    <  1:442706/300‑1 (MQ=255)                                                                            cTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGGAATCAAAGAAGTGTTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTGGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGTTCGCTAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTAc                                                                                                                                                                                                    <  1:596192/300‑1 (MQ=255)                                                                             tGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAATAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACa                                                                                                                                                                                                   <  1:188150/300‑1 (MQ=255)                                                                               ggTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCACAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGAGCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAg                                                                                                                                                                                                 <  2:687486/300‑1 (MQ=255)                                                                                gtttgtaGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAATAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGc                                                                                                                                                                                                <  1:678155/294‑1 (MQ=255)                                                                                 ttttTAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGcc                                                                                                                                                                                               <  1:303/300‑1 (MQ=255)                                                                                          ggtGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGGTGATCCAGGAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAACACCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATAGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGAAAGATGCACAGCCTGTACAAGCCGAGTACggg                                                                                                                                                                                      >  2:469773/3‑300 (MQ=255)                                                                                          gcTGTATCCCAACCGTTATCGCGTAATCAAANAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGNGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGAAACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCggg                                                                                                                                                                                      >  2:575457/1‑300 (MQ=255)                                                                                          gcTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCANCCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGNCACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCggg                                                                                                                                                                                      >  2:740175/1‑300 (MQ=255)                                                                                          gcTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCCAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCATGTACACGCCGCGTCCggg                                                                                                                                                                                      >  2:107275/1‑300 (MQ=255)                                                                                          gcTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGCTGCACAGCATGTACAAGCCGCGTCCggg                                                                                                                                                                                      >  2:469762/1‑300 (MQ=255)                                                                                          gcTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCggg                                                                                                                                                                                      >  1:419689/1‑300 (MQ=255)                                                                                           cTGTATCCCAACCGTTATTGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGc                                                                                                                                                                                     <  2:240171/300‑1 (MQ=255)                                                                                           cTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGTATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGc                                                                                                                                                                                     >  2:670282/1‑300 (MQ=255)                                                                                           cTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGc                                                                                                                                                                                     <  1:417711/300‑1 (MQ=255)                                                                                             gTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACGCCTGTTATCGCGTGCTGGGACAGATGCACAGCCTGTACAAGCCGCGTCTGGGCcg                                                                                                                                                                                   >  2:3797/1‑300 (MQ=255)                                                                                             gTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCcg                                                                                                                                                                                   >  1:266451/1‑300 (MQ=255)                                                                                               aTCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCcgcg                                                                                                                                                                                 >  2:775661/1‑300 (MQ=255)                                                                                                            tCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTatat                                                                                                                                                                    <  1:497846/300‑1 (MQ=255)                                                                                                                   aTCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTAAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACAACTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGCGCAAGCCGCGTCCGGTCCGCGTGAAAGACTCTATCGCCAtt                                                                                                                                                             >  2:676592/1‑300 (MQ=255)                                                                                                                         gaagTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGCCACCTGCTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCaaaa                                                                                                                                                       >  2:266273/1‑300 (MQ=255)                                                                                                                          aagTGGTGAAAGCCGCGCTCGGCAACCGGAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAg                                                                                                                                                      <  2:313630/300‑1 (MQ=255)                                                                                                                                        gcgcgcgGCAACCGTAAATAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAg                                                                                                                                        <  1:742245/300‑1 (MQ=255)                                                                                                                                         cgcgcgGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGt                                                                                                                                       <  1:593784/300‑1 (MQ=255)                                                                                                                                                 aaCCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCTTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGcac                                                                                                                               <  1:609578/300‑1 (MQ=255)                                                                                                                                                 aaCCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGcac                                                                                                                               <  1:244597/300‑1 (MQ=255)                                                                                                                                                 aaCCGTAAAGAGATAATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTTGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGGCTTTGcac                                                                                                                               <  2:321012/300‑1 (MQ=255)                                                                                                                                                  aCCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGcaca                                                                                                                              >  1:443794/1‑300 (MQ=255)                                                                                                                                                   ccGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCacac                                                                                                                             >  1:759836/1‑300 (MQ=255)                                                                                                                                                   ccGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCacac                                                                                                                             >  2:670345/1‑300 (MQ=255)                                                                                                                                                       aaaGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGTAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGTGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCg                                                                                                                         >  1:393000/1‑300 (MQ=255)                                                                                                                                                                atCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTNGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGc                                                                                                                >  2:688588/1‑300 (MQ=255)                                                                                                                                                                atCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTCTATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGc                                                                                                                >  2:7450/1‑300 (MQ=255)                                                                                                                                                                      aagaTTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCAc                                                                                                          <  1:713125/300‑1 (MQ=255)                                                                                                                                                                                ctGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCTTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCgg                                                                                                <  2:366218/300‑1 (MQ=255)                                                                                                                                                                                ctGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCgg                                                                                                <  2:719173/300‑1 (MQ=255)                                                                                                                                                                                  gAAATCGAAGGGCGTTTGTAGTAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCGCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGtt                                                                                              <  2:223815/300‑1 (MQ=255)                                                                                                                                                                                  gAAATCGAAGGGCGTTTGCAGTAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGtt                                                                                              <  1:757470/300‑1 (MQ=255)                                                                                                                                                                                              cGTTTGCAGGAAGCTGGAATACCGTGCCGCGTCGGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCTTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATc                                                                                  <  2:22350/300‑1 (MQ=255)                                                                                                                                                                                              cGTTTGCAGGAAGCGGGAAGACCGTGCTGCGGCAGTGTTCGCGAGAAGCGTCTTTATTCGCTTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTGCTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGTCCGCGTTAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATc                                                                                  <  2:216163/300‑1 (MQ=255)                                                                                                                                                                                                tttGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCg                                                                                <  2:710990/300‑1 (MQ=255)                                                                                                                                                                                                     aGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCg                                                                           <  1:800866/300‑1 (MQ=255)                                                                                                                                                                                                        aaGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAg                                                                        >  2:260040/1‑300 (MQ=255)                                                                                                                                                                                                         aGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGa                                                                       >  1:171271/1‑300 (MQ=255)                                                                                                                                                                                                           cGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGata                                                                     >  1:258801/1‑300 (MQ=255)                                                                                                                                                                                                           cGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGACATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGata                                                                     >  2:102265/1‑300 (MQ=255)                                                                                                                                                                                                                aTACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGAc                                                                <  1:366519/300‑1 (MQ=255)                                                                                                                                                                                                                                   gtcgctataaGCCTCTTTATTCGCTTTACTGCGACATTGTGCTCAAAGATCATTATTTTTACTCGATCATTGCCATCTACGTTTTCCGCGTGATCGTCAATGATTCTGACACCCGTTACCGCGTGCCTGGCCAGCTACACAGCCCGTACACGCCTCGTCCGTTCCGCGTGAAAGCCTCGCTCGCCGTTCCAGAGGCGAACTGCTGTCCGTCTTTGCGCACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTTCGGATCCATACCGAAGATATTGACCAGATGTCGTAGATGGgtt                                             <  2:118129/292‑2 (MQ=255)                                                                                                                                                                                                                                   gTCGCGATAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGAGGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGgtg                                             <  1:243565/300‑1 (MQ=255)                                                                                                                                                                                                                                       cgAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGc                                         <  1:306900/300‑1 (MQ=255)                                                                                                                                                                                                                                         agaAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCcg                                       <  2:758233/300‑1 (MQ=255)                                                                                                                                                                                                                                              cATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCAc                                  <  1:219063/300‑1 (MQ=255)                                                                                                                                                                                                                                                   ttATTCGGTTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACGCCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTACATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATACGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGc                             <  1:720151/300‑1 (MQ=255)                                                                                                                                                                                                                                                   ttATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGc                             <  1:250055/300‑1 (MQ=255)                                                                                                                                                                                                                                                         gATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATaa                       <  1:378553/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 gCAAAATGGTGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACg               >  1:413683/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          tGCTCAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCa      >  2:129118/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              cAAAGAGCAGCGTTTTTACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGtac  <  2:439927/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                            CGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAACTCGAAGAGCTGGGTTTTGAGGCGCTGTATCCCAACCGTTATCGCGTAATCAAAGAAGTGGTGAAAGCCGCGCGCGGCAACCGTAAAGAGATGATCCAGAAGATTCTTTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAGAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCGATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTCTGACACCTGTTATCGCGTGCTGGGCCAGATGCACAGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATCGGCCCGCACGGTGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTAC  >  NC_000913/3822877‑3823445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑