Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	284,535	T→G	A445A (GCT→GCG)	yagF →	D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	284,535	0	T	G	100.0%	148.5 / NA	41	A445A (GCT→GCG)	yagF	D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/23);  total (18/23)

GACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGG  >  NC_000913/284254‑284812                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                      gACTATCACCCGCCCGTGCGCGCCTTCCTCGCGGGCGGCGTGCAGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGATGGGCGAGAACCTTGAATGGTGGTAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGTAGGACGGCGTAGAGCAGGATGACGTGATCCTGCATACGGAGAAGGCAAAAGCGAACGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCAGGAAGGTTCGGTGATc                                                                                                                                                                                                                                                                     >  2:111067/1‑300 (MQ=255)     aTCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCAAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAGGGTTTGGTGATCAAgg                                                                                                                                                                                                                                                                 >  2:19225/1‑300 (MQ=255)                          tCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCgg                                                                                                                                                                                                                                            <  1:68574/300‑1 (MQ=255)                            cTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTNGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGcctgtctcttatacacatctcgc                                                                                                                                                                                                                                          <  1:547793/300‑22 (MQ=255)                                         tGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCAGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCAGGAAGGTTCGGTGATCAAGGCCACGGTGATCGACCCGTCGGTGGTGAGCGCAGATg                                                                                                                                                                                                                             >  2:160632/1‑300 (MQ=255)                                                 gTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGNGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATac                                                                                                                                                                                                                     <  1:575606/300‑1 (MQ=255)                                                    aTGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCATGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCCCGGCCACGGCGATCGACCCGTCTGTGGTGGGCGAAGATGGCGTATacccc                                                                                                                                                                                                                  >  2:711801/1‑298 (MQ=255)                                                     tGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATAGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGAGGACGTGATCCTGCCGCAGGAGAAGGCAAAAGCGAAAGGGCTGNCCTCGACGGTCTGCTTCCCAACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGCAGATGGCGTATaccacc                                                                                                                                                                                                                 >  2:691179/1‑300 (MQ=255)                                                         ccACCTGCGCGACCTCGGCCTTCTGCATCTGGACTCCATGACCGTGACCGGCCATACGTTTGGCTAGAACCTTGAGTGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGGCCCGTTGGTGGTGGGCGAAGAGGGCGTATACCACCacac                                                                                                                                                                                                             <  2:459664/300‑1 (MQ=255)                                                                         ggCCTGCGGCATCGGGACGCCATGACCGGGACCGGCCAGACGGTGGGCGAGAACCGTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGTCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACTTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGTGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggtgcgggtg                                                                                                                                                                                             <  1:408637/300‑1 (MQ=255)                                                                                  cATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAAAACCTTGAATGGTGGCAGGTGTCCGAGCGCCGGGCGCGCTTCCGAAAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGACAGGGCTGAACTCGAAGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAGGATGGCGTATACCACCCAACCGGCAGGGTGCGGGTGTTTGtctag                                                                                                                                                                                    >  2:304670/1‑298 (MQ=255)                                                                                                 accgtgaccgGCCAGACGGTGGGCGAGAACCTTGAATGGCGGCAGGCGTCCGAGCGCAGGGCGCGCTTCCGCCAATGCCTGCGCGAGCAGGACGGCGTAGAGCAGGATGACGTGATCCCGCCGCCGGAGAAGGCAAAAGCGAAGGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCAGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGAGGGTGTTTGTCTAGGAAGCGCaggcgatc                                                                                                                                                                     >  2:469678/1‑300 (MQ=255)                                                                                                           gCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggcgatca                                                                                                                                                           >  1:140736/1‑300 (MQ=255)                                                                                                           gCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggcgatca                                                                                                                                                           >  2:470670/1‑300 (MQ=255)                                                                                                                   gTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGaa                                                                                                                                                   >  2:507140/1‑300 (MQ=255)                                                                                                                    tGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGACTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAag                                                                                                                                                  <  1:412602/300‑1 (MQ=255)                                                                                                                                      ggtggCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGata                                                                                                                                >  1:308811/1‑300 (MQ=255)                                                                                                                                      ggtggCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCAACACACCGGCCGGGTGCGGGTGTTTGTCTAGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGAGCGata                                                                                                                                >  1:282020/1‑300 (MQ=255)                                                                                                                                      ggtggCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCAGGATGACGTGATCCTGCCGCAGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCAGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTAGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGAGTGTGCAGGGCGata                                                                                                                                >  1:281901/1‑300 (MQ=255)                                                                                                                                            aGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACTGTGTAGAGCCGGATGACGTGATTCTGCCGCCGGGGAAGGCAAAAGTGAAAGGGCTGACCTCGACGGTCTGCTTACCGACGGGCAACACCTCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCTTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGTGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCTGGAAGAGATTATGCAGGGCGATGTCAtgg                                                                                                                          <  2:103202/300‑1 (MQ=255)                                                                                                                                                    gAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGTAAGGTTCGGNGATCAAGGCCACGGCGATCGACCCGTNGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATc                                                                                                                  <  2:531259/300‑1 (MQ=255)                                                                                                                                                                                     gAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTAGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGCGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAagag                                                                                 >  2:267447/1‑300 (MQ=255)                                                                                                                                                                                               gcttAGAGCCGAATTACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAAATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGc                                                                       <  2:443539/297‑1 (MQ=255)                                                                                                                                                                                               gCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGc                                                                       >  2:351377/1‑300 (MQ=255)                                                                                                                                                                                                 gTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTc                                                                     <  1:188066/300‑1 (MQ=255)                                                                                                                                                                                                      gCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGTGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTc                                                                <  1:309666/300‑1 (MQ=255)                                                                                                                                                                                                      gCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTc                                                                <  1:54351/300‑1 (MQ=255)                                                                                                                                                                                                           aTGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCgcgc                                                           <  2:306833/300‑1 (MQ=255)                                                                                                                                                                                                           aTGACGTGATCCTGCCGCAGGAGAAGGCAAAAACGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGTGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTGTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATTGGCGGCGGGCCGTCCGGCACCGGCATGGAATAGACCTACCAGCTCACCTCCgcgc                                                           <  2:280274/300‑1 (MQ=255)                                                                                                                                                                                                           aTGACGGGATCCTGTCTCCGTAGAGGGCAAAAGCGAAAGGGCTGACCTCTACGGTCTGTTTTCCGACGTGCAACGTCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCgcgc                                                           <  2:280393/300‑1 (MQ=255)                                                                                                                                                                                                                gTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAg                                                      <  1:19327/300‑1 (MQ=255)                                                                                                                                                                                                                                  aaGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAg                                    <  1:111648/300‑1 (MQ=255)                                                                                                                                                                                                                                    ggCAAAAGCGACAGGGCTGACCTCTACGGTCTGCTTCCCGACGGGCAATATCGCGCCGGAAGGTTCGGTGATCAATGCCACGGCGATCGACCCTTCGTTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTNTCTCGGAAGCGCAGGCGATCAAGGCGNTCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGAc                                  <  2:522916/300‑1 (MQ=255)                                                                                                                                                                                                                                    ggCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAAATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGAc                                  <  1:143765/300‑1 (MQ=255)                                                                                                                                                                                                                                      cAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACgg                                <  1:421067/300‑1 (MQ=255)                                                                                                                                                                                                                                                aGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCtcat                      <  1:280419/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gggCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCGAAAGCATATCTCGTGGGGCAAGACGGTGTCGCtcatc                     <  1:64805/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gggCTGACCTCGAAGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTAGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCtcatc                     >  1:745746/1‑300 (MQ=255)                                                                                                                                                                                                                                                   gCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCAc                   <  1:324270/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  cTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTtctc    >  1:720361/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    gCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCgg  >  1:642705/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                      GACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGACGGTGTCGCTCATCACCGATGCGCGCTTCTCGG  >  NC_000913/284254‑284812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑