BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	3,822,651	(CAGGAT)_1→2	coding (252/2109 nt)	spoT →	bifunctional (p)ppGpp synthase/hydrolase SpoT

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3822646 =	4 (0.040)	76 (0.760)	59/598	0.4	95.6%	coding (247/2109 nt)	spoT	bifunctional (p)ppGpp synthase/hydrolase SpoT
*	?	NC_000913	= 3822651	3 (0.030)	76 (0.760)	59/598	0.4	95.6%	coding (252/2109 nt)	spoT	bifunctional (p)ppGpp synthase/hydrolase SpoT

Only 100 of 154 total aligned reads displayed.

AAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAA  >  NC_000913/3822347‑3822942

aaGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTAcc                                                                                                                                                                                                                                                                                                          <  1:759916/300‑1 (MQ=255)

    cGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGa                                                                                                                                                                                                                                                                                                      <  1:595548/300‑1 (MQ=255)

     gctattgctgaaggtcgtcgttaatcacaaagcgggtcgcccttgtatctgtttgaaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatcaggat                                                                                                                                                                                                                                                                                                     >  2:332194‑M1/295‑300 (MQ=255)

     gTTACCGCTATTGCTGAAGGTCGTCGTTAATNACAAAGCGGGTCGCCCTTGTATTTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGNGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCAGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTAGACTATGAAAAGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCAACTACCAGGat                                                                                                                                                                                                                                                                                                     >  2:564288/1‑300 (MQ=255)

     gTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGat                                                                                                                                                                                                                                                                                                     <  1:708218/300‑1 (MQ=255)

      ctattgctgaaggtcgtcgttaatcacaaagcgggtcgcccttgtatctgtttgaaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgcaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaaaaggatCAGGata                                                                                                                                                                                                                                                                                                    <  2:296431‑M1/7‑1 (MQ=255)

          tgctgaaggtcgtcgttaatcacaaagcgggtcgcccttgtatctgtttgaaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGa                                                                                                                                                                                                                                                                                                >  1:82553‑M1/290‑300 (MQ=255)

            ctgaaggtcgtcgttaatcacaaagcgggtcgcccttgtatctgtttgaaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAAc                                                                                                                                                                                                                                                                                              >  1:22275‑M1/288‑300 (MQ=255)

               ttGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatgg                                                                                                                                                                                                                                                                                           >  1:623837‑M2/1‑290 (MQ=255)

                tGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCGGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatgga                                                                                                                                                                                                                                                                                          >  2:516591‑M2/1‑289 (MQ=255)

                  cTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaac                                                                                                                                                                                                                                                                                        >  1:22275‑M2/1‑287 (MQ=255)

                                  aagcggttcgcccttgtatctgtttgaaagcctgaatcaactgattcacacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcccgaggggcaaacacgttcaagcggtgaaccctatntcacgcacccggtagcggttgcctgcnttctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGc                                                                                                                                                                                                                                                                        >  2:525890‑M1/266‑300 (MQ=255)

                                             ccttgtatctgtttgaaagcctgaatcaactgatgcaaacctacctgccggaagaccaaaccaagcggctgcgacaggcgtatcgcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcatttttgccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTTGCCGAGCTGGTag                                                                                                                                                                                                                                                             >  2:169205‑M1/255‑300 (MQ=255)

                                              cttgtatctgtttgaaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcngtgaaccctatatcacgcacccggtancggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTaga                                                                                                                                                                                                                                                            <  2:542326‑M1/47‑1 (MQ=255)

                                               tcgtatctgtttgaaagcctgagtcaactgattcaaacctacctgccggaagaccaaagcaaacgtctgtggcaggcgtagctcgttgcacttgatgcgcactaggggcaaacacgttcaagcggggaaccctatatcacgcacccggtagcgattgcctacattctggccgagatgaaactcgactagtaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacttactaggatCAGGAGATTGAACAGCTGTTTGGTAAAAGCGTCGCCGAGCTGGTagag                                                                                                                                                                                                                                                           >  2:213179‑M1/253‑300 (MQ=255)

                                                 gtatctgtttgaaagcctgaatcaactgattcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAggg                                                                                                                                                                                                                                                         >  1:136362‑M1/251‑300 (MQ=255)

                                                   ccTTGTATCTGTTTGAAAGCCTGAATCAACTGATGCAAACCTACCTGCCGGAAGACCAAACCAAGCGGCTGCGACAGGCGTATCGCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTTTTGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgttgccgagctggtag                                                                                                                                                                                                                                                       >  2:169205‑M2/1‑254 (MQ=255)

                                                    cTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCNGTGAACCCTATATCACGCACCCGGTANCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtaga                                                                                                                                                                                                                                                      <  2:542326‑M2/300‑48 (MQ=255)

                                                     tcgtaTCTGTTTGAAAGCCTGAGTCAACTGATTCAAACCTACCTGCCGGAAGACCAAAGCAAACGTCTGTGGCAGGCGTAGCTCGTTGCACTTGATGCGCACTAGGGGCAAACACGTTCAAGCGGGGAACCCTATATCACGCACCCGGTAGCGATTGCCTACATTCTGGCCGAGATGAAACTCGACTAGTAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACTTACTAGGATcaggagattgaacagctgtttggtaaaagcgtcgccgagctggtagag                                                                                                                                                                                                                                                     >  2:213179‑M2/3‑252 (MQ=255)

                                                       gTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagaggg                                                                                                                                                                                                                                                   >  1:136362‑M2/1‑250 (MQ=255)

                                                         aTCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagaggggg                                                                                                                                                                                                                                                 >  1:510925‑M2/1‑248 (MQ=255)

                                                                   gactcaactgattcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcaagcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgaagtgattgaagatactcccgacacctacaaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCAGAGCTGGTAGAGGGGGTGTCGAAACTTGATaa                                                                                                                                                                                                                                       <  2:337563‑M1/68‑1 (MQ=255)

                                                                     gCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGAATATGAAACGCTGATGGCGGCGTTGCTGCATGACGTGATTGAAGACACTCCCGCCACCTACAAGGATcaggatatggaacagttttttggtaaaaacgtcgacgagctggtcgggggggtgcccaactttg                                                                                                                                                                                                                                     <  2:395666‑M2/300‑65 (MQ=255)

                                                                         gactcaaCTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCAAGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGAAGTGATTGAAGATACTCCCGACACCTACAAGGATcaggatatggaacagctttttggtaaaagcgtcgcagagctggtagagggggtgtcgaaacttgataa                                                                                                                                                                                                                                 <  2:337563‑M2/297‑69 (MQ=255)

                                                                           tgattcaaacctacctgccggaagaccaaatcaagcgtctgcggcaggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGt                                                                                                                                                                                                                               >  2:786559‑M1/225‑300 (MQ=255)

                                                                             cAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgcagagctggtagagggggtgtcgaaacttgataaactc                                                                                                                                                                                                                             <  2:387487‑M2/300‑73 (MQ=255)

                                                                                   aTTCAAACCTACCTGCCGGAAGACCAAATCANGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGNTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttc                                                                                                                                                                                                                       <  2:559946‑M2/300‑79 (MQ=255)

                                                                                   aTTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAATACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCTAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttc                                                                                                                                                                                                                       >  2:70219‑M2/1‑222 (MQ=255)

                                                                                                                         ggcgtatctcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAgatgat                                                                                                                                                                                 <  2:471800‑M1/122‑1 (MQ=255)

                                                                                                                                gCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacngctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgatt                                                                                                                                                                          <  1:586751‑M2/300‑124 (MQ=255)

                                                                                                                                 tcgttgcacgtgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGACGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTCCGCAAGATGATTATGGCGa                                                                                                                                                                         <  2:359036‑M1/130‑1 (MQ=255)

                                                                                                                                      ctcGTTGCACTTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcg                                                                                                                                                                    >  2:252133‑M2/1‑171 (MQ=255)

                                                                                                                                           tgatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGa                                                                                                                                                               >  1:473284‑M1/161‑300 (MQ=255)

                                                                                                                                            gatgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGgac                                                                                                                                                              >  1:517594‑M1/160‑299 (MQ=255)

                                                                                                                                            gatactcacgaggggcaaacacgttcaagcggtgaaccctagatcacacacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTGGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGat                                                                                                                                                              >  2:378936‑M1/160‑300 (MQ=255)

                                                                                                                                             atgctcacgaggggcaaacacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGata                                                                                                                                                             <  1:241301‑M1/142‑1 (MQ=255)

                                                                                                                                                  gATGCTCGCGAGTGGCAAACACGTTCAAGCGGTGAACCCCACACCACTCACCCGTTAGCGGTTGCCTGCATTCTGGCCTAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagatggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatagtgcaggat                                                                                                                                                        >  2:468142‑M2/1‑159 (MQ=255)

                                                                                                                                                  gATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCATGATcaggatatggaacagctttttggtaaaagcggcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggat                                                                                                                                                        >  1:313607‑M2/1‑159 (MQ=255)

                                                                                                                                                  gATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggat                                                                                                                                                        >  1:216431‑M2/1‑159 (MQ=255)

                                                                                                                                                  gATACTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTAGATCACACACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagcttttgggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggat                                                                                                                                                        >  2:378936‑M2/1‑159 (MQ=255)

                                                                                                                                                     gCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaangaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcangatatc                                                                                                                                                     <  2:648911‑M2/300‑145 (MQ=255)

                                                                                                                                                              gggCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatc                                                                                                                                            <  2:352554‑M2/300‑154 (MQ=255)

                                                                                                                                                               cacgttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctacccggatCAGGATATGGAACAGCTTTTTGGTAAANGTGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGGAAGAGGCGCAGGCCGAAAAATTTCGCCAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCtcatca                                                                                                                                           <  2:185669‑M1/160‑1 (MQ=255)

                                                                                                                                                                  gttcaagcggtgaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAAc                                                                                                                                        >  2:674709‑M1/138‑300 (MQ=255)

                                                                                                                                                                             gaaccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGt                                                                                                                             <  2:72460‑M1/174‑1 (MQ=255)

                                                                                                                                                                               accctatatcacgcccccggtagtggttgcctgcattctggccgagctgaacctcgaatatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGANTATGGCGATGGTGCAGGATCTCCGCGTCATCCTCATCAAACTTGCCGACCGTAc                                                                                                                           <  2:586145‑M1/176‑1 (MQ=255)

                                                                                                                                                                               accctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgnctatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGANTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTAc                                                                                                                           <  2:735502‑M1/176‑1 (MQ=255)

                                                                                                                                                                               accctatatcaagcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAGGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTAc                                                                                                                           <  2:413658‑M1/176‑1 (MQ=255)

                                                                                                                                                                                ccctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATANACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTAcc                                                                                                                          <  1:537015‑M1/177‑1 (MQ=255)

                                                                                                                                                                                  ctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCca                                                                                                                        <  1:49440‑M1/179‑1 (MQ=255)

                                                                                                                                                                                  ctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCca                                                                                                                        <  2:686321‑M1/179‑1 (MQ=255)

                                                                                                                                                                                  ctatatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgaagtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGNGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTAATCCTCATCAAACTTGCCGACCGTACCca                                                                                                                        <  2:186158‑M1/179‑1 (MQ=255)

                                                                                                                                                                                   gAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgt                                                                                                                       <  2:72460‑M2/300‑175 (MQ=255)

                                                                                                                                                                                    ctatcacgcacccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATCTGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGACACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTAATCCTCATCAAACTTGCCGACCGTAcccaaa                                                                                                                      <  1:700287‑M1/181‑3 (MQ=255)

                                                                                                                                                                                     aCCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGNCTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgantatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtac                                                                                                                     <  2:735502‑M2/300‑177 (MQ=255)

                                                                                                                                                                                     aCCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtac                                                                                                                     <  1:45403‑M2/300‑177 (MQ=255)

                                                                                                                                                                                      cccTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgatanactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacc                                                                                                                    <  1:537015‑M2/300‑178 (MQ=255)

                                                                                                                                                                                       ccTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCTAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtntcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgngtcatcctcatcaaacttgccgaccgtaccc                                                                                                                   >  2:556477‑M2/1‑122 (MQ=255)

                                                                                                                                                                                        cTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtaccca                                                                                                                  <  1:49440‑M2/300‑180 (MQ=255)

                                                                                                                                                                                        cTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtaccca                                                                                                                  <  2:686321‑M2/300‑180 (MQ=255)

                                                                                                                                                                                        cTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGAAGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagngggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtaatcctcatcaaacttgccgaccgtaccca                                                                                                                  <  2:186158‑M2/300‑180 (MQ=255)

                                                                                                                                                                                            atCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaac                                                                                                              <  2:754730‑M2/300‑184 (MQ=255)

                                                                                                                                                                                              cccggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGACGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCAc                                                                                                            <  2:437881‑M1/191‑1 (MQ=255)

                                                                                                                                                                                                cggtagcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGc                                                                                                          <  1:217064‑M1/193‑1 (MQ=255)

                                                                                                                                                                                                    cccGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgacgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcac                                                                                                      <  2:437881‑M2/300‑192 (MQ=255)

                                                                                                                                                                                                     gcggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGc                                                                                                     <  1:526280‑M1/198‑1 (MQ=255)

                                                                                                                                                                                                     gcggttgcctgcattctggccgagatgaaacgcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGACGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGAAAGATGATTATGGCGATGGTGCAGGATATNCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACNCTGGGc                                                                                                     <  2:666333‑M1/198‑1 (MQ=255)

                                                                                                                                                                                                      cggttgcctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCt                                                                                                    >  1:715722‑M1/102‑300 (MQ=255)

                                                                                                                                                                                                      cGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgc                                                                                                    <  2:303‑M2/300‑194 (MQ=255)

                                                                                                                                                                                                            cctgcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTc                                                                                              <  1:213025‑M1/205‑1 (MQ=255)

                                                                                                                                                                                                               gcattctggccgagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGcc                                                                                           >  1:735152‑M1/93‑300 (MQ=255)

                                                                                                                                                                                                                tGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcact                                                                                          <  1:16565‑M2/300‑204 (MQ=255)

                                                                                                                                                                                                                  ccTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttc                                                                                        <  1:213025‑M2/300‑206 (MQ=255)

                                                                                                                                                                                                                          gagatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGt                                                                                >  1:168066‑M1/82‑300 (MQ=255)

                                                                                                                                                                                                                          gagatacaactcgactatgaaacgctgagggcggcgctgctgcattacgtgattgaagatactcccgccacctaccaggagCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGNGCAGGCCGAAAACTTTCGCAAGATGATTATTGCGATGGTGCAGGATATCCACGTCATCCTCATCAAACTTGCCGACCGGACCTACAACATGCNCACGCTGGGCTCACGTCGCCCGGACAAACGt                                                                                >  2:571286‑M1/82‑300 (MQ=255)

                                                                                                                                                                                                                           agatgaaactcgactatgaaacgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTNCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTc                                                                               <  2:599487‑M1/220‑1 (MQ=255)

                                                                                                                                                                                                                                 agaTGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttnccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtc                                                                         <  2:599487‑M2/300‑221 (MQ=255)

                                                                                                                                                                                                                                 agaTGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCACGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgctaagnaagaggcgcaggccgaagactttcgaaagatgattatggcgctggtgcaagatatccgcgtaatcctcatcaaacttgccgaccgtacccccaacgtgcgcacgctgggctcaattcgcccggacaaacgtc                                                                         <  2:187380‑M2/300‑221 (MQ=255)

                                                                                                                                                                                                                                               acgctgatggcggcgctgctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAAct                                                           >  1:233925‑M1/61‑300 (MQ=255)

                                                                                                                                                                                                                                                       gCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatccaacttgcagaccgtacccacaccatgcncacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactct                                                   <  2:583740‑M2/300‑243 (MQ=255)

                                                                                                                                                                                                                                                                gctgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACACCATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGccc                                          <  1:323178‑M1/257‑1 (MQ=255)

                                                                                                                                                                                                                                                                  tgcatgacgtgattgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGc                                        <  1:369269‑M1/259‑1 (MQ=255)

                                                                                                                                                                                                                                                                    ctgctgCATGACGTGATTGAAGATACTCCCGNCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccncgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagc                                      <  2:565979‑M2/300‑256 (MQ=255)

                                                                                                                                                                                                                                                                        tgcATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgc                                  <  1:369269‑M2/300‑260 (MQ=255)

                                                                                                                                                                                                                                                                          gtgattgaagatactcccgccacctaccggtatCAGGATATGGGACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGGTCCGCAATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATANCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACCTCGCCGCATCGCCCGTGNAACGCTCGAAATTTATAGCCCGCTGGCGCAc                                >  2:573370‑M1/34‑300 (MQ=255)

                                                                                                                                                                                                                                                                             attgaagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGt                             <  1:272415‑M1/270‑1 (MQ=255)

                                                                                                                                                                                                                                                                                gTGATTGAAGATACTCCCGCCACCTACCGGTATcaggatatgggacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaaggtccgcaataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatanccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacctcgccgcatcgcccgtgnaacgctcgaaatttatagcccgctggcgcac                          >  2:573370‑M2/1‑33 (MQ=255)

                                                                                                                                                                                                                                                                                 aagatactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGACGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAg                         <  2:297672‑M1/274‑1 (MQ=255)

                                                                                                                                                                                                                                                                                   aTTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgctggcgcaccgt                       <  1:272415‑M2/300‑271 (MQ=255)

                                                                                                                                                                                                                                                                                   aTTGAAGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcacgctggcgcaccgt                       <  1:22386‑M2/300‑271 (MQ=255)

                                                                                                                                                                                                                                                                                    atactcccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGGCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTa                      >  2:758203‑M1/24‑300 (MQ=255)

                                                                                                                                                                                                                                                                                       aaGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgacgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgctggcgcaccgtttag                   <  2:297672‑M2/300‑275 (MQ=255)

                                                                                                                                                                                                                                                                                        aGATACTCCCGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaantttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgctggcgcaccgtttagg                  >  2:180795‑M2/1‑25 (MQ=255)

                                                                                                                                                                                                                                                                                         cccgccacctaccaggatCAGGATATGGAACAGCTTTGTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCNAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCACACCGTTTAGGTATccac                 >  2:197253‑M1/19‑300 (MQ=255)

                                                                                                                                                                                                                                                                                          ccgccacctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATccacc                <  2:489951‑M1/283‑1 (MQ=255)

                                                                                                                                                                                                                                                                                                cctaccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTa          <  1:775503‑M1/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                                                ccGCCACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgcaagatgattatggcgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgctggcgcaccgtttaggtatccacc          <  2:489951‑M2/300‑284 (MQ=255)

                                                                                                                                                                                                                                                                                                   ccACCTACCAGGATcaggatatggaacagctttttggtaaaagcgtcgccgagctggtagagggggtgtcgaaacttgataaactcaagttccgcgataagaaagaggcgcaggccgaaaactttcgaaagatgattatgacgatggtgcaggatatccgcgtcatcctcatcaaacttgccgaccgtacccacaacatgcgcacgctgggctcacttcgcccggacaaacgtcgccgcatcgcccgtgaaactctcgaaatttatagcccgctggcgcactgtttcggtatccaccaca       <  2:242414‑M2/300‑287 (MQ=255)

                                                                                                                                                                                                                                                                                                    ccaggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCANGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAAc      >  2:708520‑M1/8‑300 (MQ=255)

                                                                                                                                                                                                                                                                                                       ggatCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGa   <  1:732988‑M1/296‑1 (MQ=255)

AAGCCGTTACCGCTATTGCTGAAGGTCGTCGTTAATCACAAAGCGGGTCGCCCTTGTATCTGTTTGAAAGCCTGAATCAACTGATTCAAACCTACCTGCCGGAAGACCAAATCAAGCGTCTGCGGCAGGCGTATCTCGTTGCACGTGATGCTCACGAGGGGCAAACACGTTCAAGCGGTGAACCCTATATCACGCACCCGGTAGCGGTTGCCTGCATTCTGGCCGAGATGAAACTCGACTATGAAACGCTGATGGCGGCGCTGCTGCATGACGTGATTGAAGATACTCCCGCCACCTACCAGGATATGGAACAGCTTTTTGGTAAAAGCGTCGCCGAGCTGGTAGAGGGGGTGTCGAAACTTGATAAACTCAAGTTCCGCGATAAGAAAGAGGCGCAGGCCGAAAACTTTCGCAAGATGATTATGGCGATGGTGCAGGATATCCGCGTCATCCTCATCAAACTTGCCGACCGTACCCACAACATGCGCACGCTGGGCTCACTTCGCCCGGACAAACGTCGCCGCATCGCCCGTGAAACTCTCGAAATTTATAGCCCGCTGGCGCACCGTTTAGGTATCCACCACATTAAAACCGAA  >  NC_000913/3822347‑3822942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.