Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	68,236	G→A	V34V (GTC→GTT)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	68,236	0	G	A	97.9%	139.6 / NA	48	V34V (GTC→GTT)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (24/23);  minor base C (1/0);  total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGAGCTGATGGG  >  NC_000913/67940‑68528                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                     aaCTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAacaacg                                                                                                                                                                                                                                                                                                   <  1:197725/300‑1 (MQ=255) aaCTCGCGACCGCCATACGCAGTCTGGTGCAGGTTCATAAAGTCCATATCGATACCGTCCCACGACAGCGCCGCGTTGAACTGGGTGGGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGATAATGTGTGCAGCCACACCACCAGACCAGCGCAATGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAacaacg                                                                                                                                                                                                                                                                                                   <  1:137452/300‑1 (MQ=255)                 gtgCAGTCTGGTTCAGGTTCATAAAGTCCATATCGCTACTGTCCCACGGCAACGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCAACAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCGACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACATGCTCGGCATGTTggg                                                                                                                                                                                                                                                                                   >  1:419049/1‑300 (MQ=255)                 gtccagtccagtcccggttcaTCAATTCCATATCGCTACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGCCTTGCAGCCCCGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCAGGGGAGAAGGTGTGCAGCCACACCACCCGCCCATCGCAACGATCGTCGTAATTCTCGTCGCGGTAAATAGTGGTGGTTTCATCCGGCGTGGTGCCGAGCGGTTTCAACACCATTTTGCAGGGCAGTTTCGCTTCCGTCTTTCA‑CGCATTCACCACATACTCGTCATGTTggg                                                                                                                                                                                                                                                                                   >  2:438278/16‑300 (MQ=255)                      gTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGACCTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCACCGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGGTTTCATCCGGCGTGGTGTCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTACGT‑ATTCAGCGCATTAACACCGTGCTCGACATGTTAGGTGaac                                                                                                                                                                                                                                                                              >  1:332396/1‑298 (MQ=255)                        cTGGTTCAGGTTCATAACGTCCCTATCGATCCTGTCCCACGGCAGTGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCAGTTGATCCGCATTTTGGCCGGGGAGAAGGTGTACAGCCACACCAACCGACCAGTGCAACGATCGTCGTAATTCGCGTCGCGGTCAATAGCGGTGATTTCATCCGGCGTGGTGCACAGCGGTTTCACCACCAGCTTGTAGGGCAGTTTCGCTTCCGT‑ATTCAGCGTATTAACAACGTGCTAGGCATGTTGGGTGACCTg                                                                                                                                                                                                                                                                            >  2:306296/1‑300 (MQ=255)                            ttcaggttcaTAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGc                                                                                                                                                                                                                                                                        <  1:702325/300‑1 (MQ=255)                                ggttcaTAAAGTCCATATCGATCCTGTCCCACGGCCGCGCCGCGTTGAACTGGGTGTGGCATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCATTGATCAACATTTTGGCCGGGGAGAAGGTNTGCAGCCACACCAACAGACCAGCGCANCGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGGGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑AGTCAGCACATTAACAACGTGCTCGGCCTGTTGGGTGCACTGACGCAgga                                                                                                                                                                                                                                                                    >  2:584911/1‑299 (MQ=255)                                         aGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGTAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGc                                                                                                                                                                                                                                                           <  1:747740/300‑1 (MQ=255)                                          gTCCATATCGATACTGTCACACGGCCGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCACCGGTTTGTTGAGCATGGCCAGGCCATTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACAACAACAGCCCAGCGCAACGATCGTAGTAATTCACGTCGCAGCAAACAGCGGTGATCTCATCCAGCGTGGTGCCCAGCGTGTCCAACACCAGTCTGCAGGGCAGTTTCGATTCCGT‑ATTCAGCGCATTACCAACGTGCTCGGTATGTTGGGTTAACCGACGCAGGGTTTCCGAGcc                                                                                                                                                                                                                                                          >  2:289969/1‑300 (MQ=255)                                              atatCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATAc                                                                                                                                                                                                                                                      <  1:327339/300‑1 (MQ=255)                                                    aTACTGTCCAACGGCAGCGCCGCGTTGAAATGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCATCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTTGAGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGTGGNTTCAACACCAGTTTGCCGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGGTCGGCATGTTGGGTGACCTGACGCAGGGTGTCCGTGCCATACAGATgc                                                                                                                                                                                                                                                >  2:756087/1‑300 (MQ=255)                                                        tgttccACGGCAGCGCCGCTTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGTAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATgctggc                                                                                                                                                                                                                                            <  2:322021/296‑1 (MQ=255)                                                         gTCCCACGGCAGCGCCGCGTTGCACTGGGTGTGGACTTGCAGCAACGGTTTGTTGANCATGGTCAGGCCGTTGATTCACATCTTGGCCGGGGAGAAGGTGTGCAGCCACACCAACAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATGACGATGATTTCATCCGGCGTGGTGCCCCGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTACGT‑CTTCAGCGCCTTACCAACGTNCTCGGCATGTTGGGTGACCTTACGCAGGGTTTACGGACCATCCAGACgctggcc                                                                                                                                                                                                                                           >  2:810756/1‑299 (MQ=255)                                                                  cAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGAc                                                                                                                                                                                                                                  <  1:517012/300‑1 (MQ=255)                                                                           gTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCATGCCGTTGATCCACATTTTGGCCGGGTAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCacac                                                                                                                                                                                                                         <  2:327752/300‑1 (MQ=255)                                                                                     gtgtGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAAAGATCGTAGTAATTCGCGTCGCGGAAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTACGT‑ATTTAGCGCATTAACAACGTGCTCGGCGTGTTGGGTGACCTGACGCAGGGTTTACGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTAATAATTa                                                                                                                                                                                                               >  1:480756/1‑300 (MQ=255)                                                                                                        gTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATNGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCg                                                                                                                                                                                            <  1:599861/300‑1 (MQ=255)                                                                                                          ttgttgAGCATGGTCAGGCCGTTGATCCACATTTTGGCCAAGGAGAAGATGTGCAGCCCCACCAACAGACCAACGCAACGCTCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCAGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTCCGCTTACGT‑ATTCAGCGCCTTAAAAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTACGGGCCCTCCAGATGCTGGCTGCCAATGACAAACCCCACTTAATAATCATCAAAAATCGTCGCTATCgta                                                                                                                                                                                          >  1:342971/1‑299 (MQ=255)                                                                                                               gcacATGGTCAGGCCGTTGATCCACATTTTGGCAGGGAAGAAGGTGTGCAACCACACCACCAGACCAGCGCAACAATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGACGCAGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGTATTTTCGCTTACGT‑CTTCAGCGCATTAACAACATGCTCGGTATGTTGGGTGACCTGACGCAGGGCTTACGGGCGACACAGATGCTGGCNGCCAATGACAAACCACACGTCGTAATTATCAAAAATCGCCATTATAGTATCCtc                                                                                                                                                                                     >  2:651629/4‑299 (MQ=255)                                                                                                                   aTGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGG‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACGTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATag                                                                                                                                                                                 <  1:542967/300‑1 (MQ=255)                                                                                                                   aTGGTCAGGCCGTGGATCCACATTTTGTCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCATCGCAAAGATCGTCGTAATTCGCGCCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGTGCCATACAGATGCTGGCTGCCAATGACAAACCACACTGCATAATTATCAAAAATCGTCATTATCGTGTCCTTATag                                                                                                                                                                                 <  1:228666/300‑1 (MQ=255)                                                                                                                    tGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTACGT‑ATTCAGCGCATTAACAACGTGCTCNGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTACTTATaga                                                                                                                                                                                >  1:623281/1‑300 (MQ=255)                                                                                                                                     cacaTTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGc                                                                                                                                                               <  1:210041/300‑1 (MQ=255)                                                                                                                                     cacaTTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGc                                                                                                                                                               <  2:794714/300‑1 (MQ=255)                                                                                                                                          tttggcgttgtagaAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCCTAATTCGCGTCGCTGCAAATAGCGGTGATTTCATCCTGCGTGGTGCCCATCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCTGCGCATTAACAACGTGCTCGGCATGTNGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGNCCTTATAGAGTCGCAACGGCCTGGGCAGCCt                                                                                                                                                          <  2:608016/289‑1 (MQ=255)                                                                                                                                              gCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGc                                                                                                                                                      <  1:700403/300‑1 (MQ=255)                                                                                                                                               ccggttagaAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGGTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGcc                                                                                                                                                     <  2:789712/294‑1 (MQ=255)                                                                                                                                               ccGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAANACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGcc                                                                                                                                                     <  1:640428/300‑1 (MQ=255)                                                                                                                                               ccGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGcc                                                                                                                                                     <  2:481852/300‑1 (MQ=255)                                                                                                                                                          gtgtGCAGCCACACCACCAGACCAGCGCAGCGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCAGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCAGGGGCGGAAGt                                                                                                                                          >  1:797095/1‑300 (MQ=255)                                                                                                                                                          gtatgCAGCCACACCACCAGACCAGCGCAACNATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCGTCCGGCGTGGTTCACAGCGGTCTCAACACCAGTTTGCAGGGAAGTTTCGCTCNAGT‑ATTCCGCGAATCAACAACGTGCTGGGCCGATTGGGTGACCTGACGCAGGGTTTCCGTGCAATACCGCTGCTGTCTGCCAATGACAAACCACACTTCACACTTATCACCAATCATCATTATCATGTCATTATAGAGTAGCACCGGACTAGGCAGCCtacgtatggggagcagt                                                                                                                                          >  2:613356/4‑284 (MQ=255)                                                                                                                                                                        ccccagaccagCGCCACGATCGTCATACTTCGCGTCGCAACAAATAGCAGCGACTTCATCCGACGTGGTGCCCAGCGATTTCCACAACAGCTTGCAGGGAAGTTTCGCTTACGT‑ATTCAGCGCACTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCCTACAGATGCTGGCTGCCAATGACCACCCCCACTTCATAATTCGCAAACATCATCGTTATCGTGTCCTTATAGAGTCGCAACGGCCTGGTCATCCTTTGCCATAGCGGAAGCTGGACGATAgtgtc                                                                                                                            >  2:755207/3‑299 (MQ=255)                                                                                                                                                                                 agCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGgcgc                                                                                                                   <  1:558410/300‑1 (MQ=255)                                                                                                                                                                                  gcgcAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCt                                                                                                                  >  1:667627/1‑300 (MQ=255)                                                                                                                                                                                               cgtAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGc                                                                                                     <  1:271766/300‑1 (MQ=255)                                                                                                                                                                                                      cgcgtcgcgGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCATCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAgc                                                                                              <  1:499719/300‑1 (MQ=255)                                                                                                                                                                                                                      gCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTc                                                                              >  1:274996/1‑300 (MQ=255)                                                                                                                                                                                                                                     ggCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATGCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCGGACGCAGGGTTTCCGNGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGGTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTg                                                               <  1:591994/300‑1 (MQ=255)                                                                                                                                                                                                                                        gtggtgCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTgttc                                                            >  1:547860/1‑297 (MQ=255)                                                                                                                                                                                                                                                     gTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATGGTGTTGTTCGGCGCTCATCGCCCATTGNTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGc                                               >  2:651627/1‑300 (MQ=255)                                                                                                                                                                                                                                                         cAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAggg                                           <  1:430382/300‑1 (MQ=255)                                                                                                                                                                                                                                                               gcgagatgtgtataagagacagtCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTtct                                     >  1:92828/23‑300 (MQ=255)                                                                                                                                                                                                                                                                aGTTTGCAGGGCAGTTTCGCTTACGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTTTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTACAGGGttttttc                                    >  2:236374/1‑297 (MQ=255)                                                                                                                                                                                                                                                                        gggCAGNTTCGCTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTNACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCAc                            <  2:728376/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   cTTCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCAttttt                 >  1:745006/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     tCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTg               >  2:681643/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     tCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGAGAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTg               >  2:843125/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     tCCGT‑ATTCAGCGCATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTACCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTg               >  1:476791/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   cATTAACAACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGGGCTGATggg  <  1:505830/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                     AACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGT‑ATTCAGCGCATTAACGACGTGCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTGCTGAGCTGATGGG  >  NC_000913/67940‑68528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑