Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	68,236	G→A	V34V (GTC→GTT)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	68,236	0	G	A	98.1%	164.9 / NA	53	V34V (GTC→GTT)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (23/28);  minor base C (1/0);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTG  >  NC_000913/67948‑68515                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                         aCCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGc                                                                                                                                                                                                                                                                              <  2:555325/300‑1 (MQ=255)    gCCATGTGCAGTCTGGTTCAGGTTCCTAAAGTCCATATCGATACTGTCCAACGGCAGCGCCGCGTTGAACTGGGTGTGGACTTGCAGCAACGGTTTGTTGCGCATGGTAAGGCCGTTGATCCACNTTTTGGCCGGGGAGAAGGTGTGCAGCNAAACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCTGGCATGGTGCCAAGCGGTTTACACACTAGTTTGCAGGGCAGTCTAGCTTCCTTATTCATCGCCTTAACAACGC‑GCTCGGCATg                                                                                                                                                                                                                                                                           >  2:563407/1‑300 (MQ=255)    gCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATg                                                                                                                                                                                                                                                                           >  1:806506/1‑300 (MQ=255)         gtgCCGTCTGGTTCCGGTTCATACAGTCCATATCGCTACTGTCCCCCGGCAGCACCGCGTTGAACTGGGTGTAGAATTGCAGCCCAGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACACTTTGGCAGGGGAGAAGGTATGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAGTTCGCGTCGCGGCAAATAGCAGTGCGTTCATCCGGCGTAGTGCCAAGCGGTTTCAACCCCAGTTTGCAGGGCAGTTTCGCTTCCGTCTTCCGTGCATTAACCACGT‑GCTCGGCATGTTgga                                                                                                                                                                                                                                                                      >  2:482692/1‑299 (MQ=255)          tgCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGt                                                                                                                                                                                                                                                                     <  1:854957/300‑1 (MQ=255)          tgCAGTCTGGTTCAGGGGCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGTAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGt                                                                                                                                                                                                                                                                     <  1:189479/300‑1 (MQ=255)            cAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACTGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGa                                                                                                                                                                                                                                                                   <  2:825712/300‑1 (MQ=255)             aGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGAc                                                                                                                                                                                                                                                                  <  1:566811/300‑1 (MQ=255)              gTCTAGTTCAGGTTCATCAAGTCCATATCGATCCTGTCCCACGGCCTCGCCACGTTGCCCTGAGTGTGGAATTGCCGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCAAATTTTGGCAAAGGAGAAGGTGTGCAGCCCCACCACCAGACCAGCGCAACGATCGTCGTACTTCGCGTCGCGGCAAATGGCGGTGATTTAATCCGGCGTGGTGCCAAACGGTTTCAACACTAGTTTGCAGGGCAGTTTAGCTTCCGTATTCAGCGCCTTAACACCGT‑GCTCTGCGTGTTgtatgaac                                                                                                                                                                                                                                                                 >  1:107476/1‑293 (MQ=255)                    ttcaggttcaTAAAGTCCATATTGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGc                                                                                                                                                                                                                                                           <  1:295108/300‑1 (MQ=255)                    ttcaggttcaTAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGc                                                                                                                                                                                                                                                           <  2:442795/300‑1 (MQ=255)                    ttcaggntcaTAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACNGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGTAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGc                                                                                                                                                                                                                                                           <  2:703173/300‑1 (MQ=255)                        ggttcaTAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAggg                                                                                                                                                                                                                                                       <  1:57962/300‑1 (MQ=255)                         gttcaTAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAggat                                                                                                                                                                                                                                                      >  1:800255/1‑298 (MQ=255)                                  gTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGCACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGGAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCTTATTCANCGCATTCCCAACGT‑GCTCGGCATGTTGGGTGACCTGACACAGGGTTTNCGTGcc                                                                                                                                                                                                                                             >  2:708887/1‑300 (MQ=255)                                      atatCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATAc                                                                                                                                                                                                                                         <  1:539270/300‑1 (MQ=255)                                            aaaCTGTCACATGGCAACACCGCGTTGAACTGGGTGGGGGATGGCAGCAACGGTTTGTTAAGCATGGGCATGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCGACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGTTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACAT‑GCTCGGCATGTTGGGTGACCTGGCGTAGGGTTTCCGGGCCATACAAATgc                                                                                                                                                                                                                                   <  2:36927/298‑1 (MQ=255)                                            aTACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATgc                                                                                                                                                                                                                                   >  1:658628/1‑300 (MQ=255)                                                 gTCCCACGGCATCGCCGCGTTGCACTGGGTGTGGACTTGCAGCAACGGTTTGTTGAGTATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACGAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCCTACGGCGTGGTGCCCAGAGGTTTCACCACCATTTTGCAGGGCAGTTTCTATTACGTATTCAGCGCATTACCAACGT‑GCTCAGGATGTTGGGTGACCTTACGCAGGGTTTACGGACCATCCAGACgctggct                                                                                                                                                                                                                              >  2:515150/1‑300 (MQ=255)                                                 gTCCCACGGCAGCGCCGCGTTGCACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCAACAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCCTCCGGCGTGGTGTCCAGCGGTTTCACCACCAGTTTGCAGGGCAGTTTCGATTCCGTACTCAGCGCATTACCAAAGT‑GCTCGGTATGTTGGGTGACCTGACGCAGAGTTTCCAGACCATACAGATgctggct                                                                                                                                                                                                                              >  1:328587/1‑300 (MQ=255)                                                       cGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCAGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAAt                                                                                                                                                                                                                        >  1:798140/1‑300 (MQ=255)                                                          cAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCATACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGAc                                                                                                                                                                                                                     <  1:13226/300‑1 (MQ=255)                                                                           gggTGTGGAATTGCAGCAACGGTTTGTTGAGNATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGNCAAATAGCTACGATTTCATCCGGTGTGGTGCCCAGCGGTTTAACCACCAGTTTGCAGGGCAGCTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCCCGGCATGTTGGGTGACCTAACGCAGGGTTTCCGGACCATACAGCTGCTGGCTGCCCATGACAAACCACACtgaataac                                                                                                                                                                                                    >  2:593919/1‑293 (MQ=255)                                                                                  gccttGCCGCACCGGTTTGTTGCACCTGGTCAGGCCGTTGATCCACCTTTTGGCCGGGGCGAAGGTGTGCCGCCACCCCACCCGACCAGCGCAACGATCGTCATAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGCAGTGCCCCGTGGTTTCAAAACCAGTTCGCAGGGCAGTTTCGCTTCCGTATTCAGTGCATTAACAACGT‑ACTAGGCACATTGGGTGACCTGACGTTGGGTTTCCGGGCAGTACAAATTATGGTTGCCAATGACCAACCACACCTCATCGTTATCaaa                                                                                                                                                                                             >  2:496621/4‑300 (MQ=255)                                                                                                  ttgttgAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGNGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCTGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGAGGCTGGCTGCCAATGACAAACCACACTTCAGAATTATCAAAAATCGTCATTATCgtg                                                                                                                                                                             <  1:571989/300‑1 (MQ=255)                                                                                                           aTGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATag                                                                                                                                                                    <  1:141385/300‑1 (MQ=255)                                                                                                           aTGGTCAGGCCGTTGAGCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATag                                                                                                                                                                    <  1:843410/300‑1 (MQ=255)                                                                                                             ggTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATagag                                                                                                                                                                  <  1:74694/300‑1 (MQ=255)                                                                                                                    ccGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAAc                                                                                                                                                           <  1:56332/300‑1 (MQ=255)                                                                                                                             cacaTTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGc                                                                                                                                                  <  1:290297/300‑1 (MQ=255)                                                                                                                                 ttttGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGcc                                                                                                                                              <  1:798858/300‑1 (MQ=255)                                                                                                                                  tttGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTNGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGNCCTTATAGAGTCGCAACGGCCTGGGCAGCCt                                                                                                                                             <  2:617009/300‑1 (MQ=255)                                                                                                                                      gCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAANTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTANCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGc                                                                                                                                         <  2:788214/300‑1 (MQ=255)                                                                                                                                       ccGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATNCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGcc                                                                                                                                        <  2:634983/300‑1 (MQ=255)                                                                                                                                                          ccACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGAt                                                                                                                     <  1:668661/300‑1 (MQ=255)                                                                                                                                                               ccaccaGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGAAAATAGCGGTGATGTCATCCAGCGTGGTGCCCAGCAGTTTCCCCACCCGTTTGCAAAGCAGTTTCGCTTCCGTATTCCGCGCATTAAC‑ACCTCACTCAACACACTGTGTGACCTGACGCAGGACCTCCGGACCACACAGATGCTGGCTGCCAACGCCACACCACACTTACTAATTCTCAACAATCGTAGTTATCGTGTCCTTATCGAGTCGCAACGGCCTGGGCCGCCCGTCCCGGAGCGGACGTTGGCAAATAgtgc                                                                                                                >  1:444230/1‑299 (MQ=255)                                                                                                                                                                ccccagaccagCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGCGACTTCCTCCGGCGTGGTGCCCAGCGATTTCCCCACCAGTTTGCAAGGCAGTTTCGCTTACGTATTCAGCGCATTAAAAACGT‑GCTCGGCATGTTGGGTGAACTGACGCAGGGTTTCCGGGCCGTACAGATGCTGGCTGCCAATGACCAACCACACTTCATAATTCTCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAAAGGCCTGGGCCGCCTGTGCCGGGGCGGAAGTTGGAAGATAGtgtt                                                                                                               >  1:771609/3‑300 (MQ=255)                                                                                                                                                                         agCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAACGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGgcgc                                                                                                      <  1:823987/300‑1 (MQ=255)                                                                                                                                                                          gcgcAGCGATCGTCGTAATTCGCGTCGCGGCACATAGCGATGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCAAATGACAAACCACACTCCATAATTCTCAAAAATCGCCATTATCGTGTCCTTATAGAGTAGCGACGGCCTGGGCAGCCTGTGCAGGGGCGGAAGTTGGAGGCGAGGGTTGTTCGgcact                                                                                                     >  2:89950/1‑297 (MQ=255)                                                                                                                                                                          gcgcAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCAGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCt                                                                                                     >  1:169285/1‑300 (MQ=255)                                                                                                                                                                                      tcgtAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTg                                                                                         >  1:528152/1‑300 (MQ=255)                                                                                                                                                                                        gtAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCt                                                                                       >  1:218613/1‑300 (MQ=255)                                                                                                                                                                                              cgcgtcgcgGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAgc                                                                                 <  1:283109/300‑1 (MQ=255)                                                                                                                                                                                                         aaaTAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGc                                                                      <  1:20891/300‑1 (MQ=255)                                                                                                                                                                                                         aaaTAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTNTCCGGGCCATACAGATGCTGGCTGCCNATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGc                                                                      >  2:580040/1‑300 (MQ=255)                                                                                                                                                                                                                              gCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTgt                                                 >  1:491643/1‑299 (MQ=255)                                                                                                                                                                                                                              gCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGCCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTgt                                                 >  1:492485/1‑299 (MQ=255)                                                                                                                                                                                                                               cGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTgtt                                                <  1:665965/300‑3 (MQ=255)                                                                                                                                                                                                                                   gtgCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCt                                            >  1:398252/1‑300 (MQ=255)                                                                                                                                                                                                                                        cAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGCTCAAAGCGTTGTGCCTGCTCGCTGcgcg                                       >  2:422165/1‑297 (MQ=255)                                                                                                                                                                                                                                                 cAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAggg                              <  1:556881/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             tCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTg  >  2:241120/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             tCCGTATTCAGCGCATTAACAACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTANCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATANTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTCTTCGCTGCGCGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTg  >  2:684259/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                         ACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGGTGTGCAGCCACACCACCAGACCAGCGCAACGATCGTCGTAATTCGCGTCGCGGCAAATAGCGGTGATTTCATCCGGCGTGGTGCCCAGCGGTTTCAACACCAGTTTGCAGGGCAGTTTCGCTTCCGTATTCAGCGCATTAACGACGT‑GCTCGGCATGTTGGGTGACCTGACGCAGGGTTTCCGGGCCATACAGATGCTGGCTGCCAATGACAAACCACACTTCATAATTATCAAAAATCGTCATTATCGTGTCCTTATAGAGTCGCAACGGCCTGGGCAGCCTGTGCCGGGGCGGAAGTTGGAAGATAGTGTTGTTCGGCGCTCATCGCCCATTGCTGATAGCGGCGATAAAGCTGTTCAAAGCGTTGTGCCTGCTCGCTGCACGGTTGCAGGGTTTTCTCTACCGCACTGGCCATTTTTTG  >  NC_000913/67948‑68515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑