Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,798	T→A	R180R (CGA→CGT)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,798	0	T	A	92.5%	172.9 / NA	53	R180R (CGA→CGT)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (13/36);  minor base C (4/0);  total (17/36)
Fisher's exact test for biased strand distribution p-value = 8.13e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGG  >  NC_000913/67515‑68093                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                        aCGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCATGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGAt                                                                                                                                                                                                                                                                                         <  2:763099/300‑1 (MQ=255)  cgcgTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATg                                                                                                                                                                                                                                                                                        >  1:846335/1‑300 (MQ=255)              aCCTTGTTCCAGGACACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCATCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTg                                                                                                                                                                                                                                                                            >  1:679002/1‑300 (MQ=255)              aCCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTNCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTg                                                                                                                                                                                                                                                                            <  1:619943/300‑1 (MQ=255)              aCCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTNCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGCCCGNCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTAttatg                                                                                                                                                                                                                                                                            >  2:792904/1‑296 (MQ=255)                   gTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCCCGTTCTGTCGTTTTTCGCCGTGGCTTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGCCCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCCCCAGATCGCCAACCGCCCAGGTATTGACGGAGCAACCGAACTTGATCTGTGCGGAAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTag                                                                                                                                                                                                                                                                       >  2:833497/1‑300 (MQ=255)                      ccAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTagaaa                                                                                                                                                                                                                                                                    <  2:171916/300‑3 (MQ=255)                       cAGGAACCGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCATGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGTAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTagaaac                                                                                                                                                                                                                                                                   <  2:320701/300‑4 (MQ=255)                       cAGGAAACACTTCATCCCCAGCTCAATACGCGCTGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCNTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTagaaac                                                                                                                                                                                                                                                                   <  2:191861/300‑4 (MQ=255)                                 ttCATCNCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTTTCGTTTTTCGCCGNGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGc                                                                                                                                                                                                                                                         <  2:692680/300‑1 (MQ=255)                                           gCTCAATCCACGCCGCTTCCCGCCCGTTCTGTCGTTTTCCACCGTTGATTTGTGTTGCCGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGAACAGCGCGTTAACATCGACGTCGCTGATGGAGTTCACCACCTGCACCAGATCGACAACCGACCAGGTATTGAAGGAGAAACCGAACTTGATTTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTCTCGCCGAAACGGCAGACTTTCAGATGAAGGGTATTCTGTTTAGAAACAGCCTGACGAGTCCAGGAGc                                                                                                                                                                                                                                               >  2:823029/1‑300 (MQ=255)                                                           ttCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATNGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGc                                                                                                                                                                                                                               <  2:737090/300‑1 (MQ=255)                                                                    gTTCTGTCGTCTTTCGCCGTGGCTTTGTGTTGCAGTCGTCATGGTGTCGCAGCTTTCGTCCTCATCGACCATCGCGTTAATATTGCCGTCGCTGATAGAGTTCACCAACTGCACCAGATCGCCAAACGCCCAGGTATTGAAGGAGAAACCGAACTTGATTTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCAGATACGCTCATGGGCTTGTTTATc                                                                                                                                                                                                                      >  2:254178/1‑300 (MQ=255)                                                                                              gtgttGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCAt                                                                                                                                                                                            <  1:121134/300‑1 (MQ=255)                                                                                                          tCATGGTGTAGCAGTTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGTAGAAACCGAACTTGATCTGTGTGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGGTTAGAAACCGCCTGACGCATCCAGGAGCCGAGACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCa                                                                                                                                                                                <  2:79601/300‑1 (MQ=255)                                                                                                          tCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGTAGTTCACCACCTGCACCAGATCGCCAACTGCCCAGGTATTGACGAAGAAACCGAACTTGATCTGNGCGGCGCCTTTATCCCCCTCGGTGACNGCCCATTCACGCCTATTATCGCCCAACCGGCAGACCTCCCCATGACGGTTATCCTGTCTAGCAACCGCATTACGCATCCAGGAGCCGATACGCTCCTGGGCTTGTTTACCCTGCAAGTGGATGGTAGCGACGGCATGGTGCTGACGCa                                                                                                                                                                                >  2:559627/1‑300 (MQ=255)                                                                                                          tCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGATGACCGCCACTTCAAGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCa                                                                                                                                                                                >  1:717951/1‑300 (MQ=255)                                                                                                               gtgtAGCAGCTTTCGTACTCCTCAACCAGCGCGTTCACATCGCCGTCGCCAATGCAGTTCACCAACTGCCCCCGATCGCCCACCGCACAGGTCCCGACGGCGCCACCGAACTTGATCTGTGCGTCCACTCTATCCACATCGGTGACCGCCACTTCACGCATGTTCTCACCAACCCGGCAGACTTTCAGCTGACGTGTCTCCTGTTTAGACACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcgc                                                                                                                                                                           >  2:554788/1‑300 (MQ=255)                                                                                                               gtgtAGCAGCTTTCGTACTCATCGACCAGCGCGTTCACATCGCCGTCGCTGATGGAGTTCACCACCTGCCCCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCCTCGGTGACCGCCACTTCAAGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCATGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcgc                                                                                                                                                                           >  1:448191/1‑300 (MQ=255)                                                                                                               gtgtAGCAGCTTTCGTACTCATCGACCAGCGCGTTCACATCGCCGTCGCTGATGGAGTTCACCACCTGCCCCAGATCACCCACCGCCCAGGTATTGACGGCGAAACCGAACTTGATCTGTGCGGCACCTTTATCAACATCGGTGACCGCCACTTCAAGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcgc                                                                                                                                                                           >  1:567407/1‑300 (MQ=255)                                                                                                               gtgtAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcgc                                                                                                                                                                           <  2:352472/300‑1 (MQ=255)                                                                                                                 gtAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATGGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcgcgc                                                                                                                                                                         <  2:513087/300‑1 (MQ=255)                                                                                                                         tttCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGa                                                                                                                                                                 <  1:776141/300‑1 (MQ=255)                                                                                                                                              ctctaaccccGCCGTCGCTAATGAAGCTCACCCCCTGCACCAGATCGCCAACCGCANCGGTACTGACGGAGCAACCGAACTTGATCTATGCGGCAACCTTATCACCATCGGTGACCGCCACTTAAAGCATGTTATCGCCAAAACGAAAGACTTTCCGATAACGAATATCCCGCTTAGAAACCGCCTAACGCATCCAGGAGCCGATACGCTCATGGGCTTGTCTATCCTGACAATGACCGGTAACGNCGGCCTGTTGCTGACGCATACGCGCGCCAATAAAGCCAAACTCGCGACCGcaat                                                                                                                                            >  2:780599/10‑297 (MQ=255)                                                                                                                                                  gaCATCGACGTCGCTGAGGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGNCACTTCACGCATGTTATCGCCAAAACGGCAGACTGTCAGATGACGGGTATCCTGTTTAGAANCCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACGCGCGACCGCTATGTGc                                                                                                                                        <  2:670460/299‑1 (MQ=255)                                                                                                                                                  aacagctccGTCGTTGATGGAGTTCACCACCTGCACCAGAGCGCCAACCGCCCAGGTATGGACTGAGAAACCGAACTGGATTTGTGCGGCAACTTTATCACCAGCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGAGACGCTCATGGGCTTGTTGATCCTGCCAGTGACCAGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGGAGCCGAACTCGCGACCGCCAtgggc                                                                                                                                        <  2:332488/293‑4 (MQ=255)                                                                                                                                                  aaCATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGNCCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGc                                                                                                                                        <  1:616667/300‑1 (MQ=255)                                                                                                                                                  aaCATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGc                                                                                                                                        <  2:148794/300‑1 (MQ=255)                                                                                                                                                    cATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAg                                                                                                                                      <  1:717636/300‑1 (MQ=255)                                                                                                                                                       cGCCTTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGTAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCt                                                                                                                                   <  2:61850/300‑1 (MQ=255)                                                                                                                                                       cGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCt                                                                                                                                   <  1:434573/300‑1 (MQ=255)                                                                                                                                                            tCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTggttc                                                                                                                              <  1:475421/300‑1 (MQ=255)                                                                                                                                                            tCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTggttc                                                                                                                              <  2:73080/300‑1 (MQ=255)                                                                                                                                                                            ccaccTGCACCCGATCGCCACCCGCCCCGGTATTGCCGACGAAACCGCACTTGATCTGTTCGGCAACTTTCTCACCCCCGGTGCCCGCCCCTTCACGCATGTTCTCGCCAACCCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGACACCGCCTGACGCCTCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGCCCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGCCCGCCATGTGCAGTCTGATTCAGGTTCATACAGTCCa                                                                                                              >  2:655111/1‑300 (MQ=255)                                                                                                                                                                                     ccAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAc                                                                                                     <  1:300723/300‑1 (MQ=255)                                                                                                                                                                                     ccAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAc                                                                                                     <  2:666578/300‑1 (MQ=255)                                                                                                                                                                                          tCGCCAACCGCCCAGGTATGGACGGAGAAACCGGACTTGATCTGTGCGGCAACTTTAGCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCANCCAGGAGCCGATACGCTCATGGGCTTNTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTcc                                                                                                <  2:586445/300‑1 (MQ=255)                                                                                                                                                                                               aaCCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACgg                                                                                           <  1:129678/300‑1 (MQ=255)                                                                                                                                                                                                aCCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGc                                                                                          <  2:274984/300‑1 (MQ=255)                                                                                                                                                                                                     ccAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAgcgc                                                                                     <  2:835296/300‑1 (MQ=255)                                                                                                                                                                                                                         cGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGa                                                                 <  1:3669/300‑1 (MQ=255)                                                                                                                                                                                                                         cGAACTGGATCTGTGCGGCAACTTTAGCACCATCGGTGACCACCACCTCACGCAGACTAGCACCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGGTGATCCTACCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGa                                                                 <  2:398963/300‑1 (MQ=255)                                                                                                                                                                                                                                gATCTTTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTgcag                                                          <  2:387573/300‑1 (MQ=255)                                                                                                                                                                                                                                     gtgCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGCCGCATCCAGGAGCCGCTACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTACCGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCCGGTTCATAAAGTCCATATCGATACTGTCCCACGACAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACg                                                     >  1:647759/1‑300 (MQ=255)                                                                                                                                                                                                                                              cTTTATCACCATCGGTGACCGCCAATTCAAGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCATGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCAAACGGTAGCGCCGCGTTGCACTGGGTGTGTAATTGCCGCAACGGTTTGTTGa                                            >  2:805245/1‑300 (MQ=255)                                                                                                                                                                                                                                                      ccgtCGGGGACCGCCACTTCGCGCATGTTATCGCCAAAACGGCAGACTTGCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCNCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAGCTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTc                                    <  2:745873/297‑1 (MQ=255)                                                                                                                                                                                                                                                        aTCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGNCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAg                                  >  1:629694/1‑300 (MQ=255)                                                                                                                                                                                                                                                          cGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCATGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGc                                <  2:447431/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  gCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATc                        <  2:261739/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        tCACGCATGTTCTCGCCCAACCTGCCGACTTTCAGCTGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGCGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTTCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCCGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTACCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGAAGCAACGGTTTGTTGCGCATGGTCAGACCGTTGATCCACAtt                  >  2:652625/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          aCGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACAtttt                <  1:120060/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        cAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGNGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAgg  <  2:189592/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        cAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAgg  <  2:527835/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                        ACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGAGCATGGTCAGGCCGTTGATCCACATTTTGGCCGGGGAGAAGG  >  NC_000913/67515‑68093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑