BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,759	G→A	G193G (GGC→GGT)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,759	0	G	A	96.5%	177.0 / ‑6.6	57	G193G (GGC→GGT)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (22/33); minor base C (2/0); total (24/33)
Fisher's exact test for biased strand distribution p-value = 1.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGA  >  NC_000913/67469‑68051

cctggAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCAACATCGGTg                                                                                                                                                                                                                                                                                             >  1:263275/4‑300 (MQ=255)

     aaGCTGTTTCAGCCCGTGCAAATCTTCCAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGACACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTAGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGATGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAAACGAGCTTGATCTGTGCGGCAACTTTATCACCACTGGTGACCGc                                                                                                                                                                                                                                                                                        >  2:494281/1‑300 (MQ=255)

          gTTTCAGCCCGTGCAAATCTTCCAAGGTGGTGGTGCACGCGTGGAAGCCACCTTGTNCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCCGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGNAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACtt                                                                                                                                                                                                                                                                                   >  2:793725/1‑300 (MQ=255)

          gTTTCAGACCGTGCAAATCTTCCAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCACCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGTAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCAAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACtt                                                                                                                                                                                                                                                                                   >  1:36989/1‑300 (MQ=255)

          gTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACtt                                                                                                                                                                                                                                                                                   >  1:7027/1‑300 (MQ=255)

          gTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCACAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACtt                                                                                                                                                                                                                                                                                   >  1:452926/1‑300 (MQ=255)

          gTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTGCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGACGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCACAGGTATTGAAGGAGAAACCGAACTTGATCTGTGCGGCCACTTTATCACCATCGGTGAACGCAACtt                                                                                                                                                                                                                                                                                   >  2:765526/1‑300 (MQ=255)

             tCAGACCGTGCAAATTTTCAAAGGTGGTGTTGAACGTGTTGAAGCCACCTTGTTCCATTAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTTTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCAc                                                                                                                                                                                                                                                                                <  2:322204/300‑1 (MQ=255)

               aGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGc                                                                                                                                                                                                                                                                              <  1:111032/300‑1 (MQ=255)

                          aTCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGcc                                                                                                                                                                                                                                                                   >  1:23921/1‑300 (MQ=255)

                            cTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCaa                                                                                                                                                                                                                                                                 >  1:825983/1‑300 (MQ=255)

                               caagggtggtggtAAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCGTCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCaaaac                                                                                                                                                                                                                                                              <  2:377879/296‑3 (MQ=255)

                                              aCGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCATGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGAt                                                                                                                                                                                                                                               <  2:763099/300‑1 (MQ=255)

                                               cgcgTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATg                                                                                                                                                                                                                                              >  1:846335/1‑300 (MQ=255)

                                                           aCCTTGTTCCAGGACACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCATCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTg                                                                                                                                                                                                                                  >  1:679002/1‑300 (MQ=255)

                                                           aCCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTNCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTg                                                                                                                                                                                                                                  <  1:619943/300‑1 (MQ=255)

                                                           aCCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTNCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGCCCGNCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTAttatg                                                                                                                                                                                                                                  >  2:792904/1‑296 (MQ=255)

                                                                gTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCCCGTTCTGTCGTTTTTCGCCGTGGCTTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGCCCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCCCCAGATCGCCAACCGCCCAGGTATTGACGGAGCAACCGAACTTGATCTGTGCGGAAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTag                                                                                                                                                                                                                             >  2:833497/1‑300 (MQ=255)

                                                                   ccAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTagaaa                                                                                                                                                                                                                          <  2:171916/300‑3 (MQ=255)

                                                                    cAGGAACCGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCATGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGTAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTagaaac                                                                                                                                                                                                                         <  2:320701/300‑4 (MQ=255)

                                                                    cAGGAAACACTTCATCCCCAGCTCAATACGCGCTGCTTCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCNTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTagaaac                                                                                                                                                                                                                         <  2:191861/300‑4 (MQ=255)

                                                                              ttCATCNCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTTTCGTTTTTCGCCGNGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGc                                                                                                                                                                                                               <  2:692680/300‑1 (MQ=255)

                                                                                        gCTCAATCCACGCCGCTTCCCGCCCGTTCTGTCGTTTTCCACCGTTGATTTGTGTTGCCGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGAACAGCGCGTTAACATCGACGTCGCTGATGGAGTTCACCACCTGCACCAGATCGACAACCGACCAGGTATTGAAGGAGAAACCGAACTTGATTTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTCTCGCCGAAACGGCAGACTTTCAGATGAAGGGTATTCTGTTTAGAAACAGCCTGACGAGTCCAGGAGc                                                                                                                                                                                                     >  2:823029/1‑300 (MQ=255)

                                                                                                        ttCCAGCACGTTCTGTCGTTTTTCGCCGTGGATTTGTGTTGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATNGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGc                                                                                                                                                                                     <  2:737090/300‑1 (MQ=255)

                                                                                                                 gTTCTGTCGTCTTTCGCCGTGGCTTTGTGTTGCAGTCGTCATGGTGTCGCAGCTTTCGTCCTCATCGACCATCGCGTTAATATTGCCGTCGCTGATAGAGTTCACCAACTGCACCAGATCGCCAAACGCCCAGGTATTGAAGGAGAAACCGAACTTGATTTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCAGATACGCTCATGGGCTTGTTTATc                                                                                                                                                                            >  2:254178/1‑300 (MQ=255)

                                                                                                                                           gtgttGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCAt                                                                                                                                                  <  1:121134/300‑1 (MQ=255)

                                                                                                                                                       tCATGGTGTAGCAGTTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGTAGAAACCGAACTTGATCTGTGTGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGGTTAGAAACCGCCTGACGCATCCAGGAGCCGAGACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCa                                                                                                                                      <  2:79601/300‑1 (MQ=255)

                                                                                                                                                       tCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGTAGTTCACCACCTGCACCAGATCGCCAACTGCCCAGGTATTGACGAAGAAACCGAACTTGATCTGNGCGGCGCCTTTATCCCCCTCGGTGACNGCCCATTCACGCCTATTATCGCCCAACCGGCAGACCTCCCCATGACGGTTATCCTGTCTAGCAACCGCATTACGCATCCAGGAGCCGATACGCTCCTGGGCTTGTTTACCCTGCAAGTGGATGGTAGCGACGGCATGGTGCTGACGCa                                                                                                                                      >  2:559627/1‑300 (MQ=255)

                                                                                                                                                       tCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGATGACCGCCACTTCAAGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCa                                                                                                                                      >  1:717951/1‑300 (MQ=255)

                                                                                                                                                            gtgtAGCAGCTTTCGTACTCCTCAACCAGCGCGTTCACATCGCCGTCGCCAATGCAGTTCACCAACTGCCCCCGATCGCCCACCGCACAGGTCCCGACGGCGCCACCGAACTTGATCTGTGCGTCCACTCTATCCACATCGGTGACCGCCACTTCACGCATGTTCTCACCAACCCGGCAGACTTTCAGCTGACGTGTCTCCTGTTTAGACACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcgc                                                                                                                                 >  2:554788/1‑300 (MQ=255)

                                                                                                                                                            gtgtAGCAGCTTTCGTACTCATCGACCAGCGCGTTCACATCGCCGTCGCTGATGGAGTTCACCACCTGCCCCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCCTCGGTGACCGCCACTTCAAGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCATGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcgc                                                                                                                                 >  1:448191/1‑300 (MQ=255)

                                                                                                                                                            gtgtAGCAGCTTTCGTACTCATCGACCAGCGCGTTCACATCGCCGTCGCTGATGGAGTTCACCACCTGCCCCAGATCACCCACCGCCCAGGTATTGACGGCGAAACCGAACTTGATCTGTGCGGCACCTTTATCAACATCGGTGACCGCCACTTCAAGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcgc                                                                                                                                 >  1:567407/1‑300 (MQ=255)

                                                                                                                                                            gtgtAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcgc                                                                                                                                 <  2:352472/300‑1 (MQ=255)

                                                                                                                                                              gtAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATGGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATAcgcgc                                                                                                                               <  2:513087/300‑1 (MQ=255)

                                                                                                                                                                      tttCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGa                                                                                                                       <  1:776141/300‑1 (MQ=255)

                                                                                                                                                                                           ctctaaccccGCCGTCGCTAATGAAGCTCACCCCCTGCACCAGATCGCCAACCGCANCGGTACTGACGGAGCAACCGAACTTGATCTATGCGGCAACCTTATCACCATCGGTGACCGCCACTTAAAGCATGTTATCGCCAAAACGAAAGACTTTCCGATAACGAATATCCCGCTTAGAAACCGCCTAACGCATCCAGGAGCCGATACGCTCATGGGCTTGTCTATCCTGACAATGACCGGTAACGNCGGCCTGTTGCTGACGCATACGCGCGCCAATAAAGCCAAACTCGCGACCGcaat                                                                                                  >  2:780599/10‑297 (MQ=255)

                                                                                                                                                                                               gaCATCGACGTCGCTGAGGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGNCACTTCACGCATGTTATCGCCAAAACGGCAGACTGTCAGATGACGGGTATCCTGTTTAGAANCCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACGCGCGACCGCTATGTGc                                                                                              <  2:670460/299‑1 (MQ=255)

                                                                                                                                                                                               aacagctccGTCGTTGATGGAGTTCACCACCTGCACCAGAGCGCCAACCGCCCAGGTATGGACTGAGAAACCGAACTGGATTTGTGCGGCAACTTTATCACCAGCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGAGACGCTCATGGGCTTGTTGATCCTGCCAGTGACCAGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGGAGCCGAACTCGCGACCGCCAtgggc                                                                                              <  2:332488/293‑4 (MQ=255)

                                                                                                                                                                                               aaCATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGNCCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGc                                                                                              <  1:616667/300‑1 (MQ=255)

                                                                                                                                                                                               aaCATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGc                                                                                              <  2:148794/300‑1 (MQ=255)

                                                                                                                                                                                                 cATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAg                                                                                            <  1:717636/300‑1 (MQ=255)

                                                                                                                                                                                                    cGCCTTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGTAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCt                                                                                         <  2:61850/300‑1 (MQ=255)

                                                                                                                                                                                                    cGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCt                                                                                         <  1:434573/300‑1 (MQ=255)

                                                                                                                                                                                                         tCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTggttc                                                                                    <  2:73080/300‑1 (MQ=255)

                                                                                                                                                                                                         tCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTggttc                                                                                    <  1:475421/300‑1 (MQ=255)

                                                                                                                                                                                                                         ccaccTGCACCCGATCGCCACCCGCCCCGGTATTGCCGACGAAACCGCACTTGATCTGTTCGGCAACTTTCTCACCCCCGGTGCCCGCCCCTTCACGCATGTTCTCGCCAACCCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGACACCGCCTGACGCCTCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGCCCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGCCCGCCATGTGCAGTCTGATTCAGGTTCATACAGTCCa                                                                    >  2:655111/1‑300 (MQ=255)

                                                                                                                                                                                                                                  ccAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAc                                                           <  2:666578/300‑1 (MQ=255)

                                                                                                                                                                                                                                  ccAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATAc                                                           <  1:300723/300‑1 (MQ=255)

                                                                                                                                                                                                                                       tCGCCAACCGCCCAGGTATGGACGGAGAAACCGGACTTGATCTGTGCGGCAACTTTAGCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCANCCAGGAGCCGATACGCTCATGGGCTTNTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTcc                                                      <  2:586445/300‑1 (MQ=255)

                                                                                                                                                                                                                                            aaCCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACgg                                                 <  1:129678/300‑1 (MQ=255)

                                                                                                                                                                                                                                             aCCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGc                                                <  2:274984/300‑1 (MQ=255)

                                                                                                                                                                                                                                                  ccAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAgcgc                                           <  2:835296/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                      cGAACTTGATCTGTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGa                       <  1:3669/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                      cGAACTGGATCTGTGCGGCAACTTTAGCACCATCGGTGACCACCACCTCACGCAGACTAGCACCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGGTGATCCTACCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGa                       <  2:398963/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                             gATCTTTGCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTgcag                <  2:387573/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                  gtgCGGCAACTTTATCACCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCCTGCCGCATCCAGGAGCCGCTACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTACCGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCCGGTTCATAAAGTCCATATCGATACTGTCCCACGACAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACg           >  1:647759/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                           cTTTATCACCATCGGTGACCGCCAATTCAAGCATGTTATCGCCAAAACGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAAACCGCATGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACGACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCAAACGGTAGCGCCGCGTTGCACTGGGTGTGTAATTGCCGCAACGGTTTGTTGa  >  2:805245/1‑300 (MQ=255)

CCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCCCCAGCTCAATACGCGCCGCTTCCAGCACGTTCTGTCGTTTTTTGCCGTGGATTTGTGTGGCAGGCGTCATGGTGTAGCAGCTTTCGTACTCATCGACCAGCGCGTTAACATCGCCGTCGCTGATGGAGTTCACCACCTGCACCAGATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCGGCAACTTTATCGCCATCGGTGACCGCCACTTCACGCATGTTATCGCCAAATCGGCAGACTTTCAGATGACGGGTATCCTGTTTAGAGACCGCCTGACGCATCCAGGAGCCGATACGCTCATGGGCTTGTTTATCCTGCCAGTGACCGGTAACCACGGCATGTTGCTGACGCATACGCGCGCCAATGAAGCCGAACTCGCGACCGCCATGTGCAGTCTGGTTCAGGTTCATAAAGTCCATATCGATACTGTCCCACGGCAGCGCCGCGTTGAACTGGGTGTGGAATTGCAGCAACGGTTTGTTGA  >  NC_000913/67469‑68051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`