Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	83,296	T→C	E78E (GAA→GAG)	leuA ←	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	83,296	0	T	C	100.0%	304.3 / NA	86	E78E (GAA→GAG)	leuA	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (50/36);  total (50/36)

GCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAAT  >  NC_000913/83001‑83589                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      gCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCa                                                                                                                                                                                                                                                                                                   >  2:360726/1‑300 (MQ=255)      gtgtAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAg                                                                                                                                                                                                                                                                                              >  1:51770/1‑300 (MQ=255)      gtgtAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAg                                                                                                                                                                                                                                                                                              >  2:473095/1‑300 (MQ=255)      gtgtAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTAAGGGCGCGTTTCACCACATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGCATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCAGCAACATCAATATCTTTCTCCACGCAg                                                                                                                                                                                                                                                                                              >  2:335504/1‑300 (MQ=255)      gtgtAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCACGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAg                                                                                                                                                                                                                                                                                              >  2:153008/1‑300 (MQ=255)          aGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAg                                                                                                                                                                                                                                                                                          >  1:807544/1‑300 (MQ=255)                cGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACNTCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACg                                                                                                                                                                                                                                                                                    <  1:640037/300‑1 (MQ=255)                cGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACg                                                                                                                                                                                                                                                                                    <  1:487455/300‑1 (MQ=255)                  gtgtCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGcg                                                                                                                                                                                                                                                                                  >  2:154269/1‑299 (MQ=255)                  gtgtCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTAAGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGAGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGcg                                                                                                                                                                                                                                                                                  >  2:153683/1‑299 (MQ=255)                    gtCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGcgca                                                                                                                                                                                                                                                                                >  1:95139/1‑297 (MQ=255)                    gtCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTACACGCAGCGAGCTAACGcgca                                                                                                                                                                                                                                                                                >  1:38807/1‑297 (MQ=255)                         gAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATAcgc                                                                                                                                                                                                                                                                           >  1:718661/1‑300 (MQ=255)                             gTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCt                                                                                                                                                                                                                                                                       >  2:416756/1‑300 (MQ=255)                                    gtggtggCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTa                                                                                                                                                                                                                                                                >  1:435120/1‑300 (MQ=255)                                       gtggCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAAcc                                                                                                                                                                                                                                                             <  1:573343/300‑1 (MQ=255)                                         ggCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAACTTGGTGGNGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTACACGCAGCGAGCTAACGCGCATATGCGGCTGTTTTTAActtg                                                                                                                                                                                                                                                           >  2:218970/1‑297 (MQ=255)                                                gCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTTAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTggcgagcg                                                                                                                                                                                                                                                    >  2:82799/1‑300 (MQ=255)                                                      aTCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTTGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGATCGATGGtt                                                                                                                                                                                                                                              <  1:358528/300‑1 (MQ=255)                                                           cgcTTCGACCACTCGCTCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCAc                                                                                                                                                                                                                                         <  1:133455/300‑1 (MQ=255)                                                           cgcTTCGACCACTCGCGCCAGATCGTCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGTGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCAc                                                                                                                                                                                                                                         <  2:457712/300‑1 (MQ=255)                                                           cgcTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGTCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGACGCGTTGCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGATTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCAc                                                                                                                                                                                                                                         <  2:458255/300‑1 (MQ=255)                                                           cgcTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCTGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCAc                                                                                                                                                                                                                                         <  1:756764/300‑1 (MQ=255)                                                           cgcTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCAc                                                                                                                                                                                                                                         <  1:728354/300‑1 (MQ=255)                                                                     aCTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCaaaa                                                                                                                                                                                                                               <  1:751300/300‑1 (MQ=255)                                                                       tCGCGCAAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATc                                                                                                                                                                                                                             <  2:733425/300‑1 (MQ=255)                                                                                         gggTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCACGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACggg                                                                                                                                                                                                           >  1:788720/1‑300 (MQ=255)                                                                                           gtgtACGCCCGGCATCTTCGCAAGAACATTCACAATCATCGGTGCAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCACCCAGCGTGCTGCGCCACTTGGTGGCGATGTGCATTGGCGAAATGGCAATAAAGGTATGAATACGTACAGCTTCAGAGACTTTCAGGGCTTCGGCCGACACGTCGAGATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGATGTTTTTACCCTGGCAAGCGCTGGTTTGCACTGATTCAAAATCACCCGGCGAAAACACggggg                                                                                                                                                                                                         >  1:50486/1‑299 (MQ=255)                                                                                           gtgtACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGa                                                                                                                                                                                                         >  1:422240/1‑300 (MQ=255)                                                                                           gtgtACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCAACATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATTTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCCCCCGGCGAAGAAACGGGGa                                                                                                                                                                                                         >  1:388480/1‑300 (MQ=255)                                                                                              tACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGCCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGNATACGGAAGGCTTCGGCGACTTTCAGNGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAAc                                                                                                                                                                                                      <  2:567091/300‑1 (MQ=255)                                                                                                     ggCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTc                                                                                                                                                                                               <  1:821416/300‑1 (MQ=255)                                                                                                        aTCTTCGCAAGAAAATTCAACATCATCGGTGGAATTACGGGCGCGTTTCACCATAGAGATAAAGAGGGCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCAt                                                                                                                                                                                            <  2:7685/300‑1 (MQ=255)                                                                                                                   aaaaTTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGANGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAacac                                                                                                                                                                                 <  1:601381/300‑1 (MQ=255)                                                                                                                   aaaaTTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAGGAAACGGGGAAACCGACTTCCATCACGTCAacac                                                                                                                                                                                 <  1:689576/300‑1 (MQ=255)                                                                                                                        tCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATa                                                                                                                                                                            <  2:469138/300‑1 (MQ=255)                                                                                                                                  cGGTGTAATTACTTGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGTTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAggg                                                                                                                                                                  <  1:192826/300‑1 (MQ=255)                                                                                                                                    gtgtAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGNGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGNGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGcc                                                                                                                                                                >  2:802055/1‑300 (MQ=255)                                                                                                                                    gtgtAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGcc                                                                                                                                                                <  1:356784/300‑1 (MQ=255)                                                                                                                                         aTTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATTCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAgcgc                                                                                                                                                           <  2:449861/300‑1 (MQ=255)                                                                                                                                                       tCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGTGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGttttt                                                                                                                                             >  2:157591/1‑300 (MQ=255)                                                                                                                                                       tCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGttttt                                                                                                                                             >  1:169915/1‑300 (MQ=255)                                                                                                                                                         aCCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGTAGTTtttct                                                                                                                                           >  2:149384/1‑300 (MQ=255)                                                                                                                                                         aCCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCAAGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttct                                                                                                                                           >  2:110429/1‑300 (MQ=255)                                                                                                                                                          ccATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCNGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTNGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttctt                                                                                                                                          >  2:682235/1‑300 (MQ=255)                                                                                                                                                          ccATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttctt                                                                                                                                          >  1:35627/1‑300 (MQ=255)                                                                                                                                                          ccATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttctt                                                                                                                                          >  2:729534/1‑300 (MQ=255)                                                                                                                                                                agatagCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCacac                                                                                                                                    >  1:289375/1‑300 (MQ=255)                                                                                                                                                                      cgcgTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGc                                                                                                                              <  1:688339/300‑1 (MQ=255)                                                                                                                                                                          ttCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGACATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGc                                                                                                                          >  2:535411/1‑300 (MQ=255)                                                                                                                                                                           tcgttcacttcatCCAGCGTGCTGCGCAGCTTGTTTGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGTCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGTTTGcc                                                                                                                         <  2:431655/288‑1 (MQ=255)                                                                                                                                                                                tacttcatCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTGCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTaa                                                                                                                    <  2:5491/293‑1 (MQ=255)                                                                                                                                                                                cacttcatCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCATGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTaa                                                                                                                    <  1:426440/293‑1 (MQ=255)                                                                                                                                                                                 acttcatCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGCTGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGAAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCGGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAAc                                                                                                                   >  2:99068/7‑300 (MQ=255)                                                                                                                                                                                 acttcatCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCTATCACGTCAACACCCATACGCTCAAGGGCAAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCATGTAAc                                                                                                                   >  2:99018/7‑300 (MQ=255)                                                                                                                                                                                 acttcatCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCGGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGNTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCCCACTCAAGCTTGCNTGTAAc                                                                                                                   >  2:717301/7‑300 (MQ=255)                                                                                                                                                                                    tgtataagagacaggcgcAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGTCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGcc                                                                                                                <  2:721367/286‑1 (MQ=255)                                                                                                                                                                                         cAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATGGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCTGCCGCCACGTCGATNTCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTCTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTc                                                                                                           <  2:751971/300‑1 (MQ=255)                                                                                                                                                                                           gCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCAc                                                                                                         <  2:721645/300‑1 (MQ=255)                                                                                                                                                                                             gTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCg                                                                                                       >  1:301796/1‑300 (MQ=255)                                                                                                                                                                                                  gcgcAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATCCGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGccctgtctcttataca                                                                                                  >  1:738701/1‑286 (MQ=255)                                                                                                                                                                                                       gCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAAtg                                                                                             >  1:726490/1‑300 (MQ=255)                                                                                                                                                                                                          tgttggCGATGTGCATTGGCTAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCATGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTgg                                                                                          <  2:17476/297‑1 (MQ=255)                                                                                                                                                                                                          tggtggCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTgg                                                                                          >  2:484668/1‑300 (MQ=255)                                                                                                                                                                                                                               aaGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGTGCAATGTGGTATCGAAAATAATGACTTGCt                                                                     >  2:124847/1‑300 (MQ=255)                                                                                                                                                                                                                                   ggCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCANGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTNTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCt                                                                 >  2:807296/1‑300 (MQ=255)                                                                                                                                                                                                                                   ggCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTNTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCt                                                                 >  2:641819/1‑300 (MQ=255)                                                                                                                                                                                                                                            ggTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTNATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGNTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTg                                                        >  2:683867/1‑300 (MQ=255)                                                                                                                                                                                                                                            ggTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTg                                                        >  1:796089/1‑300 (MQ=255)                                                                                                                                                                                                                                                tGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTGACGCGCATACGCGGCTGTTTTTAACCTTGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTc                                                    <  2:301918/300‑1 (MQ=255)                                                                                                                                                                                                                                                   aTACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCAAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCtt                                                 >  2:166270/1‑300 (MQ=255)                                                                                                                                                                                                                                                     aCGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCTGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGt                                               >  1:25745/1‑300 (MQ=255)                                                                                                                                                                                                                                                      cGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGtc                                              <  1:744170/300‑1 (MQ=255)                                                                                                                                                                                                                                                              ttCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCCTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTag                                      <  2:151265/300‑1 (MQ=255)                                                                                                                                                                                                                                                                cGGCGACTTTCATGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTagag                                    <  1:428091/300‑1 (MQ=255)                                                                                                                                                                                                                                                                cGGCGACTTGCATGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTagag                                    <  2:230488/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     aCTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTACACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCt                               >  2:310176/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          cAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGATCGATGGTTTGCACTGATTCAAAACCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCtt                          <  2:51628/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      cgccACGTCGATATCTTTCTCCACTCCGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGANACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAGGGGCCAGCGCAATTTGCGGTTTTTCTTTCACACTCAAGCTTGCCTGTAACACCTGTTCACCGTCGCGCAATGTGGTAGTGAAAATAACGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATaaaaa              <  2:747170/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          aCGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAAcc          <  1:364561/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            gTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAAccca        >  2:276401/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                            gTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAAccag        >  2:835541/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                            gTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGCTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCcg        >  2:517088/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            gTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCATGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCATCGCTTCGGGCATAAAAAAAcacg        >  2:835517/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                             tCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCcgc       >  1:571335/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  atCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAAt  >  1:500450/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      GCATGGTGTAGCCCACGGTGTCCGGAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCATCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAAT  >  NC_000913/83001‑83589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑