Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	75,707	C→T	L531L (CTG→CTA)	sgrR ←	DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	75,707	0	C	T	98.8%	287.1 / NA	85	L531L (CTG→CTA)	sgrR	DNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (43/41);  total (44/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑CAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAA‑TGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCAATGTTGTAGCAGTGGCACTTCGCATAAATGTGCGAAAACAGAGAAGTCCAGCGGCAGGGTAAAGTTGGCGCTGTTTAGCCAGATATCACTTTCGATCTCTCCTGTATGCCACTGATCGTAGTCGATCTCTTTGATTTTCA  >  NC_000913/75412‑76002                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                          aGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGc                                                                                                                                                                                                                                                                                                       <  2:532979/300‑1 (MQ=255) aGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGc                                                                                                                                                                                                                                                                                                       <  1:488318/300‑1 (MQ=255)  gTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTNAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑CACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGcc                                                                                                                                                                                                                                                                                                      >  1:594766/1‑300 (MQ=255)       aaCGGGTTTAGCGAAAACTGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTTGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCa                                                                                                                                                                                                                                                                                                 <  2:103507/300‑1 (MQ=255)         cGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCCACAATCCCGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAACCGAGGGTATTTATGTG‑TCGGCCGCGCATa                                                                                                                                                                                                                                                                                               >  1:465823/1‑300 (MQ=255)          gggTTTAGCGAAAGCGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCTTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTATTGATTTTGTTATC‑AACCAGCAATCATGGATCCGTTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATAc                                                                                                                                                                                                                                                                                              <  2:10438/300‑1 (MQ=255)          gggTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATAc                                                                                                                                                                                                                                                                                              <  1:284404/300‑1 (MQ=255)                   gAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGcc                                                                                                                                                                                                                                                                                     <  2:489971/300‑1 (MQ=255)                         gggCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGaa                                                                                                                                                                                                                                                                               <  1:133152/300‑1 (MQ=255)                             gcTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAACAACCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCAGAGGGTATTCATACG‑TAGGCCGCACATACTGCGTTGTCCCTGAATGAt                                                                                                                                                                                                                                                                           >  1:75854/1‑300 (MQ=255)                             gcTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTAAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGACGAGGGTATTCCTGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGAt                                                                                                                                                                                                                                                                           >  1:560393/1‑300 (MQ=255)                                gtgCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGGTTTTGTTCTC‑AACCCGCAATTATGGATCCGGTGGCGCAAACCAAGCTGATTTAAAATTGAACCAGCCGAGGGTATTCATGCG‑TAGGACGCGCATACTGCGTTTCCCCCGAATGATCAg                                                                                                                                                                                                                                                                        >  2:16828/1‑300 (MQ=255)                                gtgCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCCGCAATCCTGGATCCGGTGGCGCAAACCCCGCTGATTTAAAATCGAACCAACCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAg                                                                                                                                                                                                                                                                        >  1:666589/1‑300 (MQ=255)                                gtgCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGTGCAAACCACGATGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAg                                                                                                                                                                                                                                                                        >  1:431022/1‑300 (MQ=255)                                gtgCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTAAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCCCAAACCACGCTGATTTCAACTCGAACCAAACGAGGGTATTCCTGCG‑TAGGCCGCGCATCCTGCGTTGCCCCTGAATGATCAg                                                                                                                                                                                                                                                                        >  2:514797/1‑300 (MQ=255)                                   cacaGCAACAGCAGGGGCAGACATGTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTTAGAAGGAGCCTCAAATCCCTTCGCCGGCGTGATCCGGATCANGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGACCAGCCa                                                                                                                                                                                                                                                                     <  1:588621/300‑1 (MQ=255)                                     cagcaacagcaGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAAACAAGCTGATTTCAAATCGAACCAGCCGAGGGTATTCCGGCG‑TCGGACGCGCATCCTGCGTTGCCCCTGAATGATTAGTCAgt                                                                                                                                                                                                                                                                   >  2:518163/1‑300 (MQ=255)                                     cagcaacagcaGGGGCAGACATTTTTTTAACACTTTGCACCTAAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑CACCCGCAATCCTGGATCCGGTGGCGCAAACCACGGTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGATACGCATACTGCGTTTCCACTGAATGATCAGCCAgt                                                                                                                                                                                                                                                                   >  2:308152/1‑300 (MQ=255)                                       gcaacagcaGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTGTC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCCTGCG‑TCGGCCGCGCATACTGCGTTGCCCCTGAATGATCAACCAgtgg                                                                                                                                                                                                                                                                 >  1:148771/1‑300 (MQ=255)                                       gcaacagcaGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTCTC‑CACCCACAATCCTGGATCCAGTGGCGCAAACCCCGCTGATTTAAAATCAAACCAGCCGAGGGTATTCCTGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAgtgg                                                                                                                                                                                                                                                                 >  1:858105/1‑300 (MQ=255)                                       gcaacagcaGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑CACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCCTGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCCgtgg                                                                                                                                                                                                                                                                 >  2:814129/1‑300 (MQ=255)                                        caacagcaGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAgtggt                                                                                                                                                                                                                                                                <  2:774511/300‑1 (MQ=255)                                          acagcaGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAgtggtgg                                                                                                                                                                                                                                                              <  1:690686/300‑2 (MQ=255)                                            agcagGGGCAGACACTTTTTTAACACTTTGCACCTCAAAAAAGATTGGCAAAGGGCTTGAGAGGGAGCCTCAAATCCCTTCGCCGGCGGTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAgtggtggat                                                                                                                                                                                                                                                            <  2:117470/300‑4 (MQ=255)                                                 gggCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTTAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGGCGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAgtggtggataagcg                                                                                                                                                                                                                                                       <  2:224359/300‑9 (MQ=255)                                                 gggCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCATCGCACGCGTACGCGTNGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAACCAgtggtggataagcg                                                                                                                                                                                                                                                       <  2:193490/300‑9 (MQ=255)                                                 gggCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAgtggtggataagcg                                                                                                                                                                                                                                                       <  1:795640/300‑9 (MQ=255)                                                          tttttttAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGGTTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCAGAGGGTATTTATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCAAgtggtggataagcggcaacattg                                                                                                                                                                                                                                              >  2:162605/1‑283 (MQ=255)                                                          tttttttAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAgctgtctcttatacacatctcgc                                                                                                                                                                                                                                              <  1:813995/300‑23 (MQ=255)                                                            tttttAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAgtggtggataagcggcaccattgct                                                                                                                                                                                                                                            <  2:838969/300‑20 (MQ=255)                                                             ttttAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TCGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAgtggtggataagcggcaccattgctt                                                                                                                                                                                                                                           >  1:451390/1‑280 (MQ=255)                                                                    acTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGGTTTTGGTATC‑AACCCGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCG‑TCGGCCGCACACACTGCNTTGCCCCCGAATGATCAGCAAGTGGTG‑GATAAGCGGCACCATTGCTTNGCTGGc                                                                                                                                                                                                                                   >  2:714039/1‑300 (MQ=255)                                                                             cTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTg                                                                                                                                                                                                                          <  1:743958/300‑1 (MQ=255)                                                                                          ggCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTc                                                                                                                                                                                                             <  1:415858/300‑1 (MQ=255)                                                                                                ggACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTCTC‑CACCAGCCATCATGGATCCGGTGGCGCAAACCACGCTTATTTAAAATCGAACCAGCCGAGGGTATTTCTGCG‑TAGGCCGCGCATACCGCGTTGCCCCCGAATGATCAGCAAGTGGTG‑GATAAGCGGCACCCTTGCTTTGCTGGCGACCAGTTGCTGGCCCCCGTTTG‑CCaag                                                                                                                                                                                                      >  1:472126/1‑298 (MQ=255)                                                                                                ggACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGAAAACCACGCTGATTTAAAATCGACCCAACCGAGGGTATTCATTCG‑TAGGCCGCGCCTACTGCCTTGCCCCTGAATGATCAGACAGTGGTG‑NATAAGCGGCACCATTGATTTTCTGGCGACCAGTTGCTGGCACAAGTTCG‑CCAgg                                                                                                                                                                                                      >  2:634322/1‑299 (MQ=255)                                                                                                          aGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTAAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTACC‑AACCAACCACCCTGGATCCAGTGGAGCAAACCAGGCCGATTTAAAATCGAACCAGCCGAGGGTATTCCTGCGTCAG‑CCGCGCATCCTGCGTTTCCCATGAAGGATCAGCCAGTGGTGGATCAA‑CGGAAACACTGCTTTGCTGGCGACCAGTTGCTGGCACCCGTTCG‑CCAggtttacttcgc                                                                                                                                                                                            >  2:148524/1‑289 (MQ=255)                                                                                                                                 gCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCCTGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGNATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTTCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCg                                                                                                                                                                     >  2:745540/1‑300 (MQ=255)                                                                                                                                 gCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGAACAGTTGCTGGCACCAGTTCG‑CCAGGTTCCTTTCGCCATTGCGCCAGCgggaggcgtcg                                                                                                                                                                     >  1:540468/1‑290 (MQ=255)                                                                                                                                 gCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAACAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAACGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGATTCATCTCGCCATTGCGCCAGCGGGCGGCGTCg                                                                                                                                                                     >  2:22262/1‑300 (MQ=255)                                                                                                                                           gATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGGTTTTGTTCTC‑CACCAGCAATCATGGATCCGGTGGCGCAAACCACACTGATTTAAAATCGAACCAGCCGGGGGTATTCATGCG‑TAGGCCACGCATCCTGCGTTGCCCCTGAATGGTCCGCCAATGGTG‑GATAAGCGGCACCATTGTTTTGCTTGCGACCAGTTGCTGGCACCCGTTCG‑CCCGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGt                                                                                                                                                           >  1:153653/1‑300 (MQ=255)                                                                                                                                           gATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCCACCATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATNAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGt                                                                                                                                                           >  1:597814/1‑300 (MQ=255)                                                                                                                                                       cGGGTATTTTCTCAGCGCACGCGTACGCGTTGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGTTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAAtac                                                                                                                                               <  1:50327/300‑3 (MQ=255)                                                                                                                                                       cGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAAtac                                                                                                                                               <  1:156262/300‑3 (MQ=255)                                                                                                                                                         ggTATTTTCTCAGCGCATGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTCTCCAACCA‑CAATCCTGGATCCGGTGGCGCAAAACACGCTGATTTCCAACCGAACCCACCGAGGGTACTCCTGCG‑TAGGCCGAGCATACTGCGGTGCCCCTGCATTATCCGCCAGTGGTTGATAAA‑CGGCCCCATTGCTTTGCTTGCGCCCAATTTCTGGCCCCCGTTCG‑CCAAGTTCATTTCGCCATTGTGCAAGCGGGTGGTGTCGGCTTGCCaatcgatcagaacacaa                                                                                                                                             >  2:77604/1‑284 (MQ=255)                                                                                                                                                         ggTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTCTC‑CACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGATGGTATTCATGCG‑TAGGCCGCGCATACTTCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAAtacag                                                                                                                                             >  1:752530/1‑296 (MQ=255)                                                                                                                                                         ggTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAAtacag                                                                                                                                             <  1:828306/300‑5 (MQ=255)                                                                                                                                                          gTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAAtacagt                                                                                                                                            <  2:720851/300‑6 (MQ=255)                                                                                                                                                          gTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAAtacagt                                                                                                                                            <  1:643854/300‑6 (MQ=255)                                                                                                                                                            aTTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAAtacagtgt                                                                                                                                          <  2:528479/300‑8 (MQ=255)                                                                                                                                                                  tcAGCGCACGCGTACGCTTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGc                                                                                                                                    <  1:311840/300‑1 (MQ=255)                                                                                                                                                                  tcAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAANCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTNGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGc                                                                                                                                    <  2:682785/300‑1 (MQ=255)                                                                                                                                                                  tcAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGc                                                                                                                                    <  1:9062/300‑1 (MQ=255)                                                                                                                                                                  tcAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGc                                                                                                                                    <  2:774949/300‑1 (MQ=255)                                                                                                                                                                           gcgtacgcgtGGCACCCCGTTGAGAATGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAACCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGAACAGTTGCTGGCACCAGTTCG‑CCAGGTTCCTTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGACCGGAATACAATGTTGGCGCAGCGGCAcc                                                                                                                           >  2:438743/1‑299 (MQ=255)                                                                                                                                                                                 gcgtGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGAGCAAACCACGCTGATTTAAAATCGAACCAGACGAGGGTATTCATGCG‑TAGGCCGAGCATACTGCTTTGCCCCCGAATGATCAGCCAGCGGTG‑GATAAGCGGCACCATTGTGTTGCTTGCGACCAGTTACCGGCACCAGTTCGACCAG‑TTCCTTTCGCCATTGTGCCAGCGGGCGGTGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAATGGCAcctcacac                                                                                                                     >  2:258229/1‑293 (MQ=255)                                                                                                                                                                                  cgtGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACACTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTTGAGCAGCGGCACCTCGCaca                                                                                                                    <  2:81554/300‑3 (MQ=255)                                                                                                                                                                                    tGGCACCCCGTTGAGAACGTCGTTAGTGTATTGATTTTGTTATC‑AACCAGCAGTCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCacaag                                                                                                                  <  2:558853/300‑5 (MQ=255)                                                                                                                                                                                    tGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCacaag                                                                                                                  <  1:492765/300‑5 (MQ=255)                                                                                                                                                                                          cccGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGACATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCacaagtgcgcg                                                                                                            >  1:684066/1‑290 (MQ=255)                                                                                                                                                                                           ccGTTGAGAACGGCGTTAGTGTAGTGATTTTNTTCTC‑CACCAGCAATCCTGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAAGGTATTCATGCG‑TAGGCCGCGCCTACTNCGTTGCCCCTGAATGATCAGCCAGTGGTGGATCAG‑CGGCAACATTGCTTTGCTGGCGAACAATTTCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCAGAATACAATGTTGGAGCAGCGGCACCTCGCacaagtgcgcga                                                                                                           >  2:611462/1‑289 (MQ=255)                                                                                                                                                                                             ttggagaACGGCGTTAGTGTAGTGATGTGGTTAGC‑AACTAGCAATCATGGATCCGGTGGCGAAAACCACACTAATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGTCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCATTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCCGCACCTCGCACAAGTGCGCGaac                                                                                                         <  2:445183/297‑2 (MQ=255)                                                                                                                                                                                             gTTGAGAACGGCGTTAGTGTAGTGATTTTGTTCTC‑CACCAGCCATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAAACAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGaac                                                                                                         >  1:433068/1‑299 (MQ=255)                                                                                                                                                                                             gTTGAGAACGGCGTTAGTGTAGTGATTTTGTTCTC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCCTGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCAccccgcacaagtgcgcgaac                                                                                                         >  1:541576/1‑281 (MQ=255)                                                                                                                                                                                             gTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑CACCAGCAATCCTGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGaac                                                                                                         >  1:816629/1‑299 (MQ=255)                                                                                                                                                                                             gTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGaac                                                                                                         <  1:319181/300‑2 (MQ=255)                                                                                                                                                                                                       gCGTTAGTGTAGTGATTTTGTTATC‑CACCAGCAATCCTGGATCCGGTGGCGCAAACCACGCTGATTTCCAATCGACCCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCCGTGGTGGATCAG‑CGGCACCATTGCTTTGCCGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCACTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGCGTTGGAGCAGCGGCaactcgaacaagtgcgcgaacagcgaaaaag                                                                                               >  2:183470/1‑270 (MQ=255)                                                                                                                                                                                                       gCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCCGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑ACAGGTTCATTTCGACATTGCGCCAGCGGGCGGCGTCGGCTTGCCCGTCGATCGGAATACAATGTTGGAACAGCGGCAccccgcacaagtgcgcgaacagcgaaaaat                                                                                               >  2:400443/1‑271 (MQ=255)                                                                                                                                                                                                                                    cAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGtt                                                                   >  1:772321/1‑300 (MQ=255)                                                                                                                                                                                                                                        aaTCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGc                                                               <  1:489837/300‑1 (MQ=255)                                                                                                                                                                                                                                         aTCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGcc                                                              <  2:776934/300‑1 (MQ=255)                                                                                                                                                                                                                                               gATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGatat                                                        <  1:479975/300‑1 (MQ=255)                                                                                                                                                                                                                                                aTCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTCGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAACCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATc                                                       >  2:738872/1‑300 (MQ=255)                                                                                                                                                                                                                                                  ccGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATcgc                                                     <  1:738280/300‑3 (MQ=255)                                                                                                                                                                                                                                                          gcAAACNACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGNGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGAtc                                             <  2:699142/300‑1 (MQ=255)                                                                                                                                                                                                                                                             aaCCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGAtctct                                          >  1:313607/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    cTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCttcat                                   <  1:38236/300‑5 (MQ=255)                                                                                                                                                                                                                                                                                  aaCCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGATCTCGATCTCTCCTTCATGCCACTGATCGTAg                     >  2:399757/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   aCCAGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGTCACTGATCGTAgc                    <  1:487450/300‑2 (MQ=255)                                                                                                                                                                                                                                                                                      aGCCGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGNTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAgctga                 >  2:643764/1‑296 (MQ=255)                                                                                                                                                                                                                                                                                        ccGAGGGTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCTGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTTGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAgctgatc               <  1:347480/300‑7 (MQ=255)                                                                                                                                                                                                                                                                                              gTATTCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTg         >  1:710902/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 ttCATGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGAtt      >  1:750723/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                     tGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGAtttcca  >  1:184190/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                                     tGCG‑TAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATANCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGNtttcca  >  2:718808/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                          AGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATC‑AACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCG‑CAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAA‑TGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCG‑CCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCAATGTTGTAGCAGTGGCACTTCGCATAAATGTGCGAAAACAGAGAAGTCCAGCGGCAGGGTAAAGTTGGCGCTGTTTAGCCAGATATCACTTTCGATCTCTCCTGTATGCCACTGATCGTAGTCGATCTCTTTGATTTTCA  >  NC_000913/75412‑76002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑