Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,125,729	C→T	S265N (AGT→AAT)	glcE ←	glycolate dehydrogenase, putative FAD‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,125,729	0	C	T	100.0%	320.5 / NA	88	S265N (AGT→AAT)	glcE	glycolate dehydrogenase, putative FAD‑binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (50/38);  total (50/38)

TCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAGGCTGATTTCCCGACGCAGGCTCAGGGAGGCGCGCGGTCGCGGTAACACTTTCATTG  >  NC_000913/3125440‑3126023                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                       tCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGa                                                                                                                                                                                                                                                                                              <  1:563744/300‑1 (MQ=255)            ggTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATa                                                                                                                                                                                                                                                                                   >  1:86753/1‑300 (MQ=255)               ttGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAgg                                                                                                                                                                                                                                                                                <  1:9559/300‑1 (MQ=255)                tGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATCGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGGTTGTCCTCAGCTGTCGATTTCAGCCAGCGTAACGCACCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCAGCGCATCATTGGGTAATGAAATGCGCCATAAGGt                                                                                                                                                                                                                                                                               >  2:367067/1‑300 (MQ=255)                tGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGt                                                                                                                                                                                                                                                                               >  1:144577/1‑300 (MQ=255)                tGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGt                                                                                                                                                                                                                                                                               >  2:246251/1‑300 (MQ=255)                 gCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTa                                                                                                                                                                                                                                                                              >  2:553046/1‑300 (MQ=255)                 gCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTa                                                                                                                                                                                                                                                                              >  1:332533/1‑300 (MQ=255)                     aGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTg                                                                                                                                                                                                                                                                          >  1:375864/1‑300 (MQ=255)                      gCCTGCTCCTCAAAGTTCCGCGTACATGCGAACGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTACGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTgg                                                                                                                                                                                                                                                                         >  2:222491/1‑300 (MQ=255)                         tGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTaa                                                                                                                                                                                                                                                                      <  1:188118/300‑1 (MQ=255)                              ctcAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACgaga                                                                                                                                                                                                                                                                 <  1:99597/300‑1 (MQ=255)                                  aaGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAgaagaa                                                                                                                                                                                                                                                             >  1:559598/1‑300 (MQ=255)                                         gcgTACATGCGACCGGGGCTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGTCGACAGCGGGGCAAAGCCACTGTCTACGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAAAGCCCCGCCCCAGTCGATCAGTTACTCGCCGGGTCAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAGTGTACCTGGTAACGAGAAGAACGGCAgt                                                                                                                                                                                                                                                      >  2:94938/1‑300 (MQ=255)                                              cATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAgttgttg                                                                                                                                                                                                                                                 >  1:433149/1‑300 (MQ=255)                                                tGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAANCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAgttgttgtt                                                                                                                                                                                                                                               <  1:596876/300‑1 (MQ=255)                                                tGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAgttgttgtt                                                                                                                                                                                                                                               <  2:521760/300‑1 (MQ=255)                                                        gggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAAt                                                                                                                                                                                                                                       >  1:765357/1‑300 (MQ=255)                                                        gggTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGAGCAAAGCCACCATCTACGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTNAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGACCGGCAGTTGTTGTTCACGCAAt                                                                                                                                                                                                                                       >  2:212231/1‑300 (MQ=255)                                                               acacGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGcc                                                                                                                                                                                                                                <  1:63971/300‑1 (MQ=255)                                                                    ccgcAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAAc                                                                                                                                                                                                                           <  1:93554/300‑1 (MQ=255)                                                                    ccgcAAGGGGCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGTTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAAc                                                                                                                                                                                                                           <  1:192369/300‑1 (MQ=255)                                                                         aGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTTCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGAcc                                                                                                                                                                                                                      <  2:51456/300‑1 (MQ=255)                                                                         aGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGAcc                                                                                                                                                                                                                      <  1:683312/300‑1 (MQ=255)                                                                           ggTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCgg                                                                                                                                                                                                                    >  1:457988/1‑300 (MQ=255)                                                                               gAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTNAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAAc                                                                                                                                                                                                                >  1:608793/1‑300 (MQ=255)                                                                               gAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCAATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAAc                                                                                                                                                                                                                >  2:524350/1‑300 (MQ=255)                                                                                aGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCACGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAAcc                                                                                                                                                                                                               <  2:224457/300‑1 (MQ=255)                                                                                     ctgTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTACGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCATGCAATTGCTGCCAGAACTGACCGGCAACCTCTTc                                                                                                                                                                                                          >  2:713304/1‑300 (MQ=255)                                                                                              ctgctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAATTACTCGCCGGGTAAATCCCTCATCGGCGCCCCATTGGTTACTGAAATGCACCATAAGGTACCTGGTAACGAGCCGCATGGCAGTTGTTGTTCACGCAATTGATGCAAGAACTGACCGGCAACCTCTTAGCAACCcag                                                                                                                                                                                                 >  1:734913/1‑300 (MQ=255)                                                                                               tgctgTTGATAGCGGAATAAAGGAGCCGATAGCGGGTCAAAGCCACCATCTCCGGCACTAAAGCGGTTCGCATGACCGCCAGCGTTGCGGTCGATGCGATGGATTTGATTGTCCGCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCcagc                                                                                                                                                                                                <  2:339497/300‑1 (MQ=255)                                                                                               tgctgGTGATAGCGGAATAAATTAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGTTCGCACGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGTTAACGAGAAGAACGGCAGTTGTTGTTTACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCcagc                                                                                                                                                                                                <  2:160874/300‑1 (MQ=255)                                                                                                 ctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCTGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGNCCTCGGCTGTCGATTTCAGCCAGCTTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCcagcag                                                                                                                                                                                              <  1:607357/300‑1 (MQ=255)                                                                                                 ctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGTTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCcagcag                                                                                                                                                                                              <  1:219986/300‑1 (MQ=255)                                                                                                 ctgGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCcagcag                                                                                                                                                                                              <  1:87772/300‑1 (MQ=255)                                                                                                             gAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCgctgc                                                                                                                                                                                  >  1:94620/1‑300 (MQ=255)                                                                                                                   aGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATC‑ATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTAcc                                                                                                                                                                           <  1:164947/300‑1 (MQ=255)                                                                                                                    ggAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTAcc                                                                                                                                                                           <  1:794873/300‑1 (MQ=255)                                                                                                                       gCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGAt                                                                                                                                                                        >  1:368041/1‑300 (MQ=255)                                                                                                                            tAGCGGGGCAAAGCCACCATCTACGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGACACCCAGCAGTTCACGCGCTGCTTTTATCGATCCTTc                                                                                                                                                                   >  2:271554/1‑300 (MQ=255)                                                                                                                                 gggCAAAGCCACCATCTACGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTcgccgc                                                                                                                                                              >  1:852198/1‑300 (MQ=255)                                                                                                                                          caccaTCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACACCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGc                                                                                                                                                     <  2:241976/300‑1 (MQ=255)                                                                                                                                           accaTCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGNCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCg                                                                                                                                                    <  1:607074/300‑1 (MQ=255)                                                                                                                                               tctcCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATc                                                                                                                                                <  1:503397/300‑1 (MQ=255)                                                                                                                                                ctcCGGCACTAAAGCGGGTCGCATGACCGCCNGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGNAAATCCATCATCGGCGCATCATTGGGGAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCAGCCCTCAAGGCGGATcc                                                                                                                                               >  2:591668/1‑300 (MQ=255)                                                                                                                                                    tgCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACaa                                                                                                                                           <  1:421039/299‑1 (MQ=255)                                                                                                                                                     gCACTAAAGCGGGTCGCATGACCGCCAGTGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCGTTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAAc                                                                                                                                          >  2:98550/1‑300 (MQ=255)                                                                                                                                                     gCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAAc                                                                                                                                          >  1:497436/1‑300 (MQ=255)                                                                                                                                                      cACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACg                                                                                                                                         >  1:787445/1‑300 (MQ=255)                                                                                                                                                      cACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCCATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCACCAGTTGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTCATGAAATGCGCCCTAAGGTATCTGGTAACGCGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCATCAAGGCGGCTcccccacg                                                                                                                                         >  2:7934/1‑294 (MQ=255)                                                                                                                                                       aCTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGACACCCAGCAGTTCACGCGCTGCTTCTACCGATCCTTAGCCGCCCTCAAGGCGGCTCCACAACGc                                                                                                                                        >  2:22579/1‑300 (MQ=255)                                                                                                                                                                  tCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGNAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGt                                                                                                                             >  1:574302/1‑300 (MQ=255)                                                                                                                                                                  tCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGt                                                                                                                             >  1:22914/1‑300 (MQ=255)                                                                                                                                                                   cGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAANCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCGTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTa                                                                                                                            <  1:611325/300‑1 (MQ=255)                                                                                                                                                                       tGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTAGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACAt                                                                                                                        <  1:113559/300‑1 (MQ=255)                                                                                                                                                                         aCCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATaa                                                                                                                      >  1:168703/1‑300 (MQ=255)                                                                                                                                                                            gCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGcc                                                                                                                   <  1:562901/300‑1 (MQ=255)                                                                                                                                                                            accAGCGTTGCGTGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCTCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGcc                                                                                                                   <  2:425323/299‑1 (MQ=255)                                                                                                                                                                                 cGTTGCTGGCGATTCGATGGATTTGATTGCCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAAGCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCATGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTaa                                                                                                              <  2:117822/300‑1 (MQ=255)                                                                                                                                                                                      cGGGCCATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACTCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCTGCGCACCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACTAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGt                                                                                                         <  2:52779/300‑1 (MQ=255)                                                                                                                                                                                            atgcgatgGATTTGATTGTCCTCGGCTGTCGATTTCAGCCATCGTAACGCCCCGCCCCAGTCAATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGc                                                                                                   <  2:812413/300‑1 (MQ=255)                                                                                                                                                                                                   ggATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCNGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTNTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGtt                                                                                            >  2:688169/1‑300 (MQ=255)                                                                                                                                                                                                      tttGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGcc                                                                                         <  1:223305/300‑1 (MQ=255)                                                                                                                                                                                                       ttGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCTGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCa                                                                                        <  2:84383/300‑1 (MQ=255)                                                                                                                                                                                                                       gTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCCGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCAACTAATGAGTAATGGCTGGAGTTGCCACTCGACGATTTCACTc                                                                        >  2:377498/1‑300 (MQ=255)                                                                                                                                                                                                                          gagttCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCGGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCGTTGACCGATCCTTCGCCGCCCTCAATGCGGATCCACAACGCATTGTCGAGGTAACACAAGCCACTAATGGGTAATGGCTGGAGtctgtctcttatacacatctcgcag                                                                     <  1:438705/297‑25 (MQ=255)                                                                                                                                                                                                                           aTTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATgg                                                                    >  1:394589/1‑300 (MQ=255)                                                                                                                                                                                                                                   cagcGTAACGCCCCGCCCCAGTCTATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCTGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGc                                                            <  1:749031/300‑1 (MQ=255)                                                                                                                                                                                                                                   cagcGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGc                                                            <  1:378291/300‑1 (MQ=255)                                                                                                                                                                                                                                   cagcGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCAATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTAGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGc                                                            >  1:815902/1‑300 (MQ=255)                                                                                                                                                                                                                                     gcGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAGTGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCGGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAg                                                          >  1:24605/1‑300 (MQ=255)                                                                                                                                                                                                                                     gcGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAg                                                          >  1:21788/1‑300 (MQ=255)                                                                                                                                                                                                                                     gcGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCAATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCAATAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAg                                                          >  1:187320/1‑300 (MQ=255)                                                                                                                                                                                                                                     gcGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGACGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAAACTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGAATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAg                                                          >  1:339138/1‑300 (MQ=255)                                                                                                                                                                                                                                     gcGTAACGCCCCGCCCCAGTCGATCAGTTGCNCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCNATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGAATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAg                                                          >  2:596170/1‑300 (MQ=255)                                                                                                                                                                                                                                                cgccccAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAGGCTGATTTccc                                               >  1:735457/1‑300 (MQ=255)                                                                                                                                                                                                                                                cgccccAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTAGGCGATTTCACTAATGGCTTCTTGCAGGCTGATTTccc                                               >  2:548911/1‑300 (MQ=255)                                                                                                                                                                                                                                                 gccccAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAGGCTGATTTCCCg                                              >  1:434542/1‑300 (MQ=255)                                                                                                                                                                                                                                                       gTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAGGCTGATTTCCCGACGCAg                                        >  1:338455/1‑300 (MQ=255)                                                                                                                                                                                                                                                       gTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAAACTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCGAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAGGCTGATTTACAGACGCAg                                        >  2:267015/1‑300 (MQ=255)                                                                                                                                                                                                                                                             cAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAGGCTGATTTCCCGACGCAGGCTCAg                                  >  1:828265/1‑300 (MQ=255)                                                                                                                                                                                                                                                               gTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTCCCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCAGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGTTGGAGTTGCCACTTGGTGATTTCACTCATGGATTCTTGTATGCTGATTTCCCAACGCAGGCTCAggg                                >  2:358659/1‑300 (MQ=255)                                                                                                                                                                                                                                                               gTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAGGCTGATTTCCCGACGCAGGCTCAggg                                >  1:551967/1‑300 (MQ=255)                                                                                                                                                                                                                                                               gTTGCTCGCCGGGTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGGTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAGGCTGATTTCCCGACGCAGGCTCAggg                                <  2:527548/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          ggTAAATCCATCATCGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAGGCTGATTTCCCGACGCAGGCTCAGGGAGGCGcgcggt                     >  1:285325/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     catcGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATTGCTTCTTGCAGGCTGATTTCCCGACGCAGGCTCAGGTAGGCGCGCGGTCGCGGTAacac          <  1:92826/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       tcGGCGCATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAGGCTGATTTCCCGACGCAGGCTCAGGGAGGCGCGCGGTCGCGGTAACACtt        <  1:838440/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          gcgcATCATTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCCATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAGCATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAGGCTGATTTCCCGACGCAGGCTCAGGGAGGCGCGCGGTCGTGGTACCACTGTCa     >  2:479353/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             catcatTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAGGCTGATTTCCCGACGCAGGCTCAGGGAGGCGCGCGGTCGCGGTAACACTTTCATTg  <  1:378958/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                       TCAGTTAATTGGGTTTGCATAGCCTGCTCCTCAAAGTTCCGCGTACATGCGACCGGGGTTAAACACGCCGCAAGGGTCGAGCTGCTGTTTAAGCTGCTGGTGATAGCGGAATAAAGGAGCCGATAGCGGGGCAAAGCCACCATCTCCGGCACTAAAGCGGGTCGCATGACCGCCAGCGTTGCGGGCGATGCGATGGATTTGATTGTCCTCGGCTGTCGATTTCAGCCAGCGTAACGCCCCGCCCCAGTCGATCAGTTGCTCGCCGGGTAAATCCATCATCGGCGCATCACTGGGTAATGAAATGCGCCATAAGGTACCTGGTAACGAGAAGAACGGCAGTTGTTGTTCACGCAATTGCTGCCAGAACTGACCGGCAACCTCTTCGCCACCCAGCAGTTCACGCGCTGCTTTTACCGATCCTTCGCCGCCCTCAAGGCGGATCCACAACGCATTGTCGAAGTAACATAAGCCACTAATGGGTAATGGCTGGAGTTGCCACTCGGCGATTTCACTCATGGCTTCTTGCAGGCTGATTTCCCGACGCAGGCTCAGGGAGGCGCGCGGTCGCGGTAACACTTTCATTG  >  NC_000913/3125440‑3126023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑