Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	279,802	G→A	A25V (GCC→GTC)	insA2 ←	IS1 family protein InsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	279,802	0	G	A	96.9%	209.4 / NA	64	A25V (GCC→GTC)	insA2	IS1 family protein InsA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (39/23);  minor base C (2/0);  total (41/23)
Fisher's exact test for biased strand distribution p-value = 5.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAATCGTGTTGAGGCCAACGCCCATAATGCGGGCGGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGA  >  NC_000913/279634‑280096                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        aaaaTCGTGTTGAGGCCAACGCCCATAATGCGGGCGGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCgag                                                                                                                                                                      <  1:545928/300‑1 (MQ=11)      cGTGTTGAGGCCAACGCCCATAATGCGGGCGGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAacac                                                                                                                                                                 <  1:655836/300‑1 (MQ=17)         gtTGAGGCCAACGCCCATAATGCGGGCGGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGNGCTGATGTCCGACAGTGCTTTTGCCGNTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCGGTAAGGTGGTAGCATCACCGAGAACACaaa                                                                                                                                                              <  2:585421/300‑1 (MQ=16)         gtTGAGGCCAACGCCCATAATGCGGGCGGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCAACGAGAACACaaa                                                                                                                                                              >  1:841728/1‑300 (MQ=18)              ggCCAACGCCCATAATGCGGGCGGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTNGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGc                                                                                                                                                         >  1:572252/1‑300 (MQ=21)              ggCCAACGCCCATAATGCGGGCGGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGc                                                                                                                                                         >  1:550151/1‑300 (MQ=21)                    cGCCCATAATGCGGGCGGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTc                                                                                                                                                   >  1:57685/1‑300 (MQ=37)                      cccATCATGCGGGCGGTTACCCAGCACCCAACGCCATTCATGGCCATATCACTGATCTTCTAGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGCGAGCAGAGATAGCGCTGATGTCCGCCAATGCTTTTGCCGTTCCGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTAATAGACCCAGACGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCATTAAGTTGGCAGCATAAACGAGAaccccacttccctcattcgg                                                                                                                                                 >  2:102270/1‑282 (MQ=11)                      cccATAATGCGGGCGGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACNGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑gg                                                                                                                                                >  1:611407/1‑300 (MQ=38)                      cccATAATGCGGGCGGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑gg                                                                                                                                                >  1:363005/1‑300 (MQ=38)                      cccATAATGCGGGCGGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCAACGAGAACACAAATGGGCTAATTC‑gg                                                                                                                                                >  1:668859/1‑300 (MQ=255)                       ccATAATGCGGGCGGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCAATGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGc                                                                                                                                               >  1:557455/1‑300 (MQ=255)                            aTGCGGGCGGTGGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGTCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCACCATCCACTAAATCGGGACGGTGCTACCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTAc                                                                                                                                          <  2:371577/300‑1 (MQ=11)                                               aTCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGANGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTc                                                                                                                       >  1:578623/1‑300 (MQ=255)                                                    aCGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGcc                                                                                                                  >  1:215268/1‑300 (MQ=255)                                                        cATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCACCATACACTAAATCAGTAAGTTAGCAGCACCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAat                                                                                                              <  2:814386/300‑1 (MQ=255)                                                         aTTCCTGGCCATATCACTGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACNTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTACTTC‑GGCAGTAATTTCCTAAAAAGACATGTCTTGCCCAata                                                                                                             >  2:735290/1‑300 (MQ=255)                                                         aTTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCAACGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAata                                                                                                             >  1:454840/1‑300 (MQ=255)                                                          ttCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTATTGCCCAatat                                                                                                            >  1:716198/1‑300 (MQ=255)                                                              tGGCCATATCAATGATTTTCTGGTGCGTACCNGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTNCGCACCAACCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAACGGGCTAATTC‑GGCAGTACGTTCCTAAAAAGACACGTCCTGcccccgctcctc                                                                                                        >  2:589020/1‑291 (MQ=14)                                                               ggCCGTATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAGGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGTAGGGACAGCTGATATAAACAGAAGCCACTGGAGCACCTCAAAAACACCACCATACACTAAATCATTAAGCTAGCAACATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCa                                                                                                       <  2:138748/300‑1 (MQ=17)                                                                             ttttCTNGTGCGTACCGGGTTGATAAGCGGTGTAAGTGAACTGCAGTTGCCATGNTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATATAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGGAAGGTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATc                                                                                         <  2:708806/300‑1 (MQ=255)                                                                             ttttCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTTCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGTAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAACTCAGTAAGTTGGCAGCACCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATc                                                                                         <  2:756372/300‑1 (MQ=255)                                                                             ttttCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTNCGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATc                                                                                         <  1:616116/300‑1 (MQ=255)                                                                             ttttCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATc                                                                                         <  1:372562/300‑1 (MQ=255)                                                                              tttCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCt                                                                                        >  1:265225/1‑300 (MQ=255)                                                                              tttCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCACAATATACTCAACATGAACTAGATCt                                                                                        >  1:119243/1‑300 (MQ=255)                                                                              tttCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGAAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGacct                                                                                        >  2:837344/1‑297 (MQ=255)                                                                               ttCTTGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTCATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTg                                                                                       <  2:509230/300‑1 (MQ=255)                                                                               ttCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTg                                                                                       <  1:721115/300‑1 (MQ=255)                                                                                 cTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGt                                                                                     <  1:848237/300‑1 (MQ=255)                                                                                         tACCGGGTTGATAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTc                                                                             <  1:194374/300‑1 (MQ=255)                                                                                            cggtttGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATTGGGAGGTTGGCAGTATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGc                                                                          <  2:376786/296‑1 (MQ=255)                                                                                            cGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGc                                                                          <  1:123075/300‑1 (MQ=255)                                                                                             gggTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTCTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAACCACCATCATCCACTACATTAGTACGTTGGCAGCATCACCGAGAATACAAATGGGCTAATTCAGGC‑GTACTTTCATAAAAAGACATGTCTTGCCTAATCTAATCAAAACGTACCAGATCTGATTGTTGCTCaaat                                                                         >  2:123675/1‑297 (MQ=14)                                                                                               gTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCAGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTAAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCCTCACAGAGAACACAAATGGGCTAATTC‑GGCAGTAGGTTCCTAAAAAGACATGTCTTGCCCCATGCACTTAACATGAATTAGACCTggatgttgttccaccaa                                                                       >  2:344462/1‑285 (MQ=17)                                                                                                 tGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCACCATACACTAAATCGGGAAGGTGGTAACACCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAaac                                                                     <  2:459153/300‑1 (MQ=21)                                                                                                        cGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGAc                                                              >  1:257513/1‑300 (MQ=255)                                                                                                        cGGTGTAAGTAAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAGTCGGTAGGTTGGCAGTATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGAc                                                              <  2:306148/300‑1 (MQ=255)                                                                                                          gtgtAAGTGAACTGCAGTTGCCATGTTTTACGGCGGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCAAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCg                                                            >  1:339507/1‑300 (MQ=255)                                                                                                          gtgtAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCCCCCCCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCg                                                            >  1:455013/1‑300 (MQ=255)                                                                                                          gtgtAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCANCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCg                                                            >  1:604351/1‑300 (MQ=255)                                                                                                          gtgtAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTAAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCg                                                            >  1:191125/1‑300 (MQ=255)                                                                                                            gtAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCgag                                                          >  1:555841/1‑300 (MQ=255)                                                                                                            gtAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCAACGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCgag                                                          >  1:555296/1‑300 (MQ=255)                                                                                                                         aGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCTACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAGAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAg                                             <  1:768791/300‑1 (MQ=255)                                                                                                                         aGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAg                                             <  1:677447/300‑1 (MQ=255)                                                                                                                            tGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTc                                          <  1:329133/300‑1 (MQ=255)                                                                                                                                 gcgagatgtgtataagagacAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAac                                     >  1:400522/20‑300 (MQ=255)                                                                                                                                     ttACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCg                                 >  1:717728/1‑300 (MQ=255)                                                                                                                                          ggaGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGCAAGACACTGAAGTTCAAAACACCGAAaga                            >  1:399002/3‑300 (MQ=255)                                                                                                                                          gCAGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCCGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCAACGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAaga                            >  1:481441/1‑300 (MQ=255)                                                                                                                                            aGTGAGAGCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAgaga                          >  1:106111/1‑300 (MQ=255)                                                                                                                                                 gagCAGGGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCAACGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCCACATGAACTCGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGGGATAAtt                     >  1:520554/1‑300 (MQ=255)                                                                                                                                                 gagCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTGCTTTCCTAAAAAGAAATGTCTTGCACAATCTACTCGCCCTGAAATAGATCTGGTTGCTGCTCANCTCAACAACAGACTGAGAAAGacaaagaagcccaaaaaaacgcccgaagtaatt                     >  2:618846/1‑270 (MQ=18)                                                                                                                                                 gagCAGAGATAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAAtt                     >  1:707526/1‑300 (MQ=255)                                                                                                                                                      gagaTAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAg                >  1:363514/1‑300 (MQ=255)                                                                                                                                                       agaTAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAATAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGGTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAgg               <  2:512417/300‑1 (MQ=255)                                                                                                                                                        gaTAGCGCTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAggg              >  1:501584/1‑300 (MQ=255)                                                                                                                                                            gcgcTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCATCTAAACCACCGACCGAGAAAGACACCGAAGTTCAAAACCCCGAAAGAGATAATTGAAAGGGgtgt          >  1:171840/1‑300 (MQ=255)                                                                                                                                                            gcgcTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGgtgt          >  1:414129/1‑300 (MQ=255)                                                                                                                                                               cTGATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGc       <  1:162112/300‑1 (MQ=255)                                                                                                                                                                 gATGTCCGACAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTAAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGTAGTACTTTACTAAAAAGACGTGTCTTGCCCAATATCCTCCACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACAGAGAAAGACACTGAAGTTCACCACACCGAAAGAGATAATTGAAAGGGGTGTTGcct     >  2:7078/1‑298 (MQ=25)                                                                                                                                                                    gTCCGCCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTAAAAAACACCATCATACACTAAATCAGTAAGTTGGNAGCATCACCGAGAACACAAATGGGCTACTTC‑GGCAGTACTTTCCTACAAAGCCATGTCTTGNCCAATATACTCAAAATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGGTAATTGAAAAGGGTGTTGcctcga  >  2:671110/1‑295 (MQ=25)                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        AAAATCGTGTTGAGGCCAACGCCCATAATGCGGGCGGTTGCCCGGCATCCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCAGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCGAGAACACAAATGGGCTAATTC‑GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGA  >  NC_000913/279634‑280096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑