Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913915,1900CT34.0% 83.8 / 11.1 54intergenic (‑333/+162)lysO/aqpZL‑lysine/thialysine exporter/water channel AqpZ
Reads supporting (aligned to +/- strand):  ref base C (24/11);  new base T (0/18);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 6.93e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAA  >  NC_000913/914896‑915462
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                 
gaggACACACTACTGAAAGCGGAAGGACGAGGAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCggggg                                                                                                                                                                                                                                                                              <  1:321967/297‑1 (MQ=255)
gATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCTAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCggggg                                                                                                                                                                                                                                                                              <  2:439762/300‑1 (MQ=255)
     cacacTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAg                                                                                                                                                                                                                                                                         <  1:299526/300‑1 (MQ=255)
      acacTCCTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCCCATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAgg                                                                                                                                                                                                                                                                        >  1:560610/1‑300 (MQ=255)
       cacTACTGAAAGCTGAAGGACGATTAAAGTTGCAACTAAAAGGAAATGTTATACATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAggg                                                                                                                                                                                                                                                                       <  2:471466/300‑1 (MQ=255)
        actactGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACNAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAgggg                                                                                                                                                                                                                                                                      <  1:615619/300‑1 (MQ=255)
         ctactGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAggggg                                                                                                                                                                                                                                                                     >  1:815902/1‑300 (MQ=255)
          tactGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggc                                                                                                                                                                                                                                                                    <  1:67370/300‑1 (MQ=255)
           actGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGggc‑g                                                                                                                                                                                                                                                                  >  1:62535/1‑300 (MQ=255)
                         gACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGgccg                                                                                                                                                                                                                                                    >  1:558885/1‑300 (MQ=255)
                         gACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGACGAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGgccg                                                                                                                                                                                                                                                    >  1:558902/1‑300 (MQ=255)
                            gAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGccc                                                                                                                                                                                                                                                 <  1:797008/300‑1 (MQ=255)
                            aaGTAAAGTTGCAATTAAAAGGAAATTTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGTTGGCGGCAAAATCGCCGAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCACATGGATGTGTGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGTGGGTGCAGGGGGC‑GGCGGCGATTGGCCGccc                                                                                                                                                                                                                                                 <  2:323878/299‑1 (MQ=255)
                                                           gcagaagggataggagagTATTTGTTTGCGTTGAAAGATATTCTTGCGCTTTAATTAAAAACTGCACCGGTGTTGTTGGCGTAAAAATCGCCGAGTCGTCCCTTAAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCTTTTTACAGGGGTGTTACCTCGCGCCCGACCCGGTAGCCGTAGGGGAAAAGTCGAGGGCA‑CCGCACAGTGGCGGTTTTGTTCGCAAGAGCCTGGGGGTGCGGGGGGC‑GGCGGCGATGGGCCGCCCCTTGCGCGCTCCTTGCGCCAGTGGCAATATg                                                                                                                                                                                                                  <  2:407136/286‑1 (MQ=17)
                                                             aTAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCAGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGtt                                                                                                                                                                                                                >  1:169688/1‑300 (MQ=255)
                                                                  gAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGNCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCACGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTa                                                                                                                                                                                                           >  2:785910/1‑300 (MQ=255)
                                                                    gCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCATGAAGG‑CCGGGGCAGCCCACATGGATGTGGNCTGAGGGCGCGCTTTACAGGGATGTTACCTCGCGCCCGCCCCGGTAGCCGTAAGGGATAAGNCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGc                                                                                                                                                                                                         >  2:721924/1‑300 (MQ=255)
                                                                    gCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCACTGAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGc                                                                                                                                                                                                         >  2:472561/1‑300 (MQ=255)
                                                                           ggtatttgttttcagtgaaaaaccttCTTACGCTTTAATTACAAACTACACCGGTTGTGGTGTCGGCAAAATCGCCGAGTCGTTCCC‑GAAGGGCTGGGGCACCCCAAAGGGATGTGGGCTGGGGGCGTTTTTGAAAGGGGTGTTACCTCGCGCCCGACCAGGTAGCCGGAAGGAAGAATTAGAGGACA‑CCACCCAGCGGCGATTTTGTTCGCAAAAGCCCGGGGGGGAGGGGGGC‑GGCGGCGATTGCCCACCCCCTACGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGaa                                                                                                                                                                                                  <  2:261695/276‑1 (MQ=255)
                                                                                                        actcttgagctACAAACTACACCGATGTGGGGGGCGTCAAAGCCTCCGAGATTTTCCCGGAGGT‑CCGGGGCACCCCACGGGGATGTGGGCGGAGGGCGCGTTGTAAAGGGATGTGACCTCACGCCCGACCTGGTAGCCGGAGGGGAGAAGTCGAGGACA‑CCGCACAGCGGCGGTTNTGTTCGCGAGAGCCGGGGGGGGCGGGGGGC‑GGCGGCGATTGGCCTCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATNAAAGGAGCGCAACAAGATGATGAATCAACat                                                                                                                                                                     <  2:647948/290‑1 (MQ=255)
                                                                                                             tgaatgaCAAACTACACCGATGTTGGTGGCGTCAAAATCGTCGATGCGTTCCCGGAAGG‑CCGGGACATCCCACATGGGTGGGGGCGGGGGGCGCGTTTGACGGGGATGTTACCTCGCGCCCGACTTGGTAGCTGGAGGGGATGAGTCGAGGGCA‑CCGCGCAGCGGCGGTTTTGTTCGCAAGATCCTGGGGGTGCGGGGGGC‑GTTGTCGCTTGGCCGCCCCTTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATaac                                                                                                                                                                <  2:493845/294‑1 (MQ=255)
                                                                                                                                aTGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCCTGAAAGGAGCGCAACAAGGTGATGAATCAACATATAGCAACATCTTAACAAAAGGca                                                                                                                                             >  2:357209/1‑299 (MQ=255)
                                                                                                                                            taaaaaTCGCAGAGGCGTTCCCGGAAGG‑CCGGGACAACCCACAGGGGTGTGGGTGGGGGGCGTGTTTGACGGGGATTTTACCCCGCCCCCGACCCGGGAACGGGAGGGGAGAAGTCGAGGACA‑CCGCGCAGTGGTGGTTTTGTTCGCAAGATCTTGGGGGGGGGGGGGGT‑GGTGGCGATTGTCCGCCCCCTCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGcc                                                                                                                                 <  1:870547/298‑1 (MQ=255)
                                                                                                                                            tCAAAATCGCAGGGGTTTTCCCTAAAGG‑CTGGGGCAACCCACGGGGGTGTGGGTGGGGGGCTCGTTTTACGGGGGTGTTACTTCGCGCCCGACCCGGTAGCTGGAGGGGAGAAGTCGAGGACA‑CCGCACAGCGGCGGTTTTGTTCGCCAAATCCTGGGGGTGAGGGGGGT‑GGCGGCGGTTGCCCCCCCCCTCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGcc                                                                                                                                 <  1:862876/300‑1 (MQ=255)
                                                                                                                                                         gggcgtcccctggcgcccgttgCAGCCCACCTGGGTTTGGGTTGAGGTCGTTTTTTACGGGGATTTCACCTCGCACCCGACCCGGTAGCCGTAATGGATACGTCGAGGGCA‑CCACGCAGCGGGGGTTTTGTTCTCCGGAGCCCTGGGGTGCGGGGGGC‑GGCGTCGGTGGTCCGCCCCCGACGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCg                                                                                                                     <  2:90108/279‑1 (MQ=255)
                                                                                                                                                              tcccgaaatg‑CCGGGACAGCCCACATAGATGTGTACTGAGGGCGCGTTCTACAGGGATGTTACCCCGCGCCCGACCCGGTAGCCGGGGGGGATAGTTCGGGGGCA‑CCGCGCAGTGTCGGTTTTGTTCGCCAGATCCTGGGGGTGGGGGNGGT‑GTCGGCGGTGGTCCTCCCCCTACNCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGttaatt                                                                                                               <  2:613730/291‑1 (MQ=255)
                                                                                                                                                                                     acaTGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTCGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATTACGCTTTTCCAGCAGGGt                                                                                         >  1:476019/1‑300 (MQ=255)
                                                                                                                                                                                       aggggtgtggattgggtgcgcTTTTTCCAGGGATGTTACCTCGCGCCCGGCCCGGGAGCCGTAAGGGGGAGGTCGAGGGCA‑CCACACAGCGTCGGTTTTGTTCGCCAGGGCTTGGGGGTGCGGGGGGT‑GTTGGCGGTTGGCCTCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTcc                                                                                       <  2:344895/283‑1 (MQ=255)
                                                                                                                                                                                          ggtgtgGGCGGAGGTCGCGTTTGAAAGGGATGTTACCTCGCCCCCGACCAGGGAGCAGGAGGGGAGAAGTCG‑GGGCAACCGCGCAGCGTTGGTTTGGTTCGCAAGAACCNGGGGGGGAGGGGGGT‑GGTGGCGGTGGTCCTCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGt                                                                                    <  1:617519/298‑1 (MQ=255)
                                                                                                                                                                                          gATGTGGGCGGAGGGCGCGTTTGATGTGGATGTTACCTCGCGCCCGACCTGGTAGCCGTAGGGGAGAAGTCGGGGGCA‑CCTCGCAGTTTCGGTTTTGTTCACCAGATCCTGGGGGTGCAGGGGGC‑GGCGTCGGTTGTCCCCCCCCTCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGt                                                                                    <  1:373615/300‑1 (MQ=255)
                                                                                                                                                                                           atgtggatggAGGGCGCGTTTTACAGGGATGTTACCCCGCGCCCGCCCCGGGAGCCGGAAGGGAGAAGGCGAGGACA‑CCGCGCAGTGGCGGTTTTGTTCGCAAGGGCCTGGGGGGGAAGGGGGT‑GGCGGCGGTTGGCCGCCCCTTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTa                                                                                   <  1:130660/291‑1 (MQ=255)
                                                                                                                                                                                            tgtgGGCTGGGGGCGTGTTTTATAGGGGTTTTACCTCGCTCCCTACCCGGTAGCCGTAGGGGATAATTCGGGGGCA‑CCGCGCAGCGGCGCTTTTTTTCGCAAGGTCCTGGGGGTGGGGGGGGC‑GGCGGCGCTTGCCCACCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTaa                                                                                  <  1:680656/300‑1 (MQ=255)
                                                                                                                                                                                              tgGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAAt                                                                                >  1:738221/1‑300 (MQ=255)
                                                                                                                                                                                               gtgtggggtttgcgTTTTATAGGGGTTTTACCTCACTCCCGCCCCGTTAACCGGAAGGGAGAAGCCGAGGACC‑CCTCTCAGCGGCGGTTTTGTTCGCCAGATCCTGGGGGTG‑AGGGGGTTGGCGGCGGTTGTCCCCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATc                                                                               <  1:355418/289‑1 (MQ=255)
                                                                                                                                                                                               ggttgggggtcgtttttggtgggggtGTTACCTCGCGCCGGACCGGGTAGTCGGAGGGGAGAGGTAGAGGGCA‑CCACACGTTGGCGGTTTTGTTCGCAAGAGCCTGGGGGTGCGGGGGGT‑GGTGGTGGTGGTCCTCCCCTTTCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATc                                                                               <  1:174922/275‑1 (MQ=255)
                                                                                                                                                                                               gggttggggtcgttttttACAGGGGTTTTACCTCGCCCCCGACCTGGTAGCTGGGAGGGAGAGGTAGAGGGCA‑CCTCGCGTTGGCGGTTTTGTTCGCAAGATCCTGGGGGTGCGGGGGGG‑GGTGGCGGTTTTCCCCCCCCTCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATc                                                                               <  1:253713/286‑1 (MQ=255)
                                                                                                                                                                                                    gAGGGCACGTTCTACAGGGATGTTACCCCGCGCCCGACCCGGTAACCGGGAGGGATAATTCGGGGGCA‑CTGCGCATCGGCGGTTTTGTTCGCCAGAACCTGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGacc                                                                          <  2:93115/300‑1 (MQ=37)
                                                                                                                                                                                                          gcgTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAAAATCTTAAAAAAAGGCCTGACATTACGCCAGGACTTCTGCGTTAATTAATCAAGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGAt                                                                    >  2:493111/1‑300 (MQ=255)
                                                                                                                                                                                                                       aTGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGACTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCCGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACa                                                       >  1:122033/1‑300 (MQ=255)
                                                                                                                                                                                                                                  cgcCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGACCGGCCTTCTGCGTTGATTAATCCCGCTTTTCCAGTAGGGTCCGGTAAATCAGACCACCAATAATgccgccaataagtagcaataa                                            >  2:433053/1‑285 (MQ=25)
                                                                                                                                                                                                                                      cGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCag                                        >  1:720271/1‑300 (MQ=255)
                                                                                                                                                                                                                                       gACCTGGTAGCTGGAAGGGAGAAGTCGGGGACA‑CCCCGCATCGTCGATTTTGTTCGCCAGATCCTGGGGGGGCGGGGGGC‑GGTGTTGGTTTTCCTCCCCCTCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCaga                                       <  1:725485/300‑1 (MQ=255)
                                                                                                                                                                                                                                         cccGTTAGCCGTAAGGGATAAGTCGAGTGCC‑CCGCGCATCGTAGGTTTTGTTCGCCAGAGCCTGGGGGGGCAGGGGGC‑GGCGGCGGTGGGCCGCCCCTTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCagaag                                     <  2:499614/300‑1 (MQ=255)
                                                                                                                                                                                                                                                   gTAAGGGATAAGTCGCGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGACCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAACATGTTGCTTCGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATCACAACATCTTAAAAAAAGGCATGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTACATCAGACCACCGACAATGCCAACGACAACTGGTGCCACCCAgaggaaacaaaggt                           >  2:198981/1‑292 (MQ=25)
                                                                                                                                                                                                                                                       gggATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAggtgttt                       >  2:498229/1‑299 (MQ=255)
                                                                                                                                                                                                                                                        ggATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTCGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAACAGGCCTGACATTACGCCAGGCCTTCTGCGTTCATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGAAAATTGGCAACACCCAGAAGGACTAAAGTTGTTCt                      >  2:52301/1‑300 (MQ=38)
                                                                                                                                                                                                                                                        ggATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAAAAACATCTTCAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAACTGGTACCAACCagaaaagccaaaggtggttt                      >  2:683775/1‑284 (MQ=38)
                                                                                                                                                                                                                                                           ataagtcgaggacaccgatcagtgttggttttGTTCGCCAGAGCCCGGGGGTGAAGGGGGC‑TTTGGCGGTTGTCCTCCCCCTGCGCGCTCCTTGCGCCAGTTGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCATGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGTCCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTaa                    <  2:560877/272‑1 (MQ=255)
                                                                                                                                                                                                                                                            aaGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCATGACATTAAGCCAGGACTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGAACACCGATAATGCAGACGACAATTGGCACCACCCAGAAGAACCAGAGTTGTTCTAATg                  >  2:328175/1‑300 (MQ=255)
                                                                                                                                                                                                                                                             aagtcgataccaccccccatctgctcttttGTTCTCCAGACCCTGGGGGTGGGGGGGGT‑GGCGGTGGTTGTCCCCCCCCTCCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATg                  <  1:738459/274‑1 (MQ=255)
                                                                                                                                                                                                                                                              gTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGTGCGCTCCTTGTGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACCTTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGca                >  1:500976/1‑299 (MQ=255)
                                                                                                                                                                                                                                                              gTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCCGCAGGGTCCGGTAAATCAGACCACCAATAATGCCGACGACAATTGGCAACACCCAGAAGAACCACAGTTGTTCTAATGcc                >  1:98604/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                   gggCA‑CCGCGCAGCGGCGATTTTGTTCGCAAGAGCCCGGGGGTGCAGGGGGT‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGTGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACACTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAACGCCGACGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAgcc           >  1:204496/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                     gaaacaaagaaGCGGCGATTTTGTTCACAAGATCCTGGGGGGGCAGGGGGC‑GTCGCTGCTTGCCCCCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATANCAACATCTTAAAAAAAGGCCTGACATNACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCTGTAAATCAGACCACCGATAATGCCGCCTACAATTGGTACCACCTAGAAGAACCACAGTTGTTCTAATGCCCAgccg          <  2:583454/290‑1 (MQ=255)
                                                                                                                                                                                                                                                                             acagtggcagttttGTTCGCCAGACCCTGGGGGGGCGGGGGGT‑GGCGGCGGTTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATNAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCTAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGaa  <  2:780334/290‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                 
GATGACACACTACTGAAAGCGGAAGGACGAGTAAAGTTGCAATTAAAAGGAAATGTTATGCATAAGGAGCAGTAGAGTATTCGTTTTCATTTAAAGATATTCTTGCGCTTTAATTACAAACTGCACCGATGTTGGTGGCGTCAAAATCGCCGAGGCGTTCCCTGAAGG‑CCGGGGCAGCCCACATGGATGTGGGCTGAGGGCGCGTTTTACAGGGATGTTACCTCGCGCCCGACCCGGTAGCCGTAAGGGATAAGTCGAGGGCA‑CCGCGCAGCGGCGATTTTGTTCGCCAGAGCCCGGGGGTGCAGGGGGC‑GGCGGCGATTGGCCGCCCCCTGCGCGCTCCTTGCGCCAGTGGCAATATGTTGCTTAGCTCATGAAAGGAGCGCAACAAGATGATGAATCAACATATAACAACATCTTAAAAAAAGGCCTGACATTACGCCAGGCCTTCTGCGTTAATTAATCACGCTTTTCCAGCAGGGTCCGGTAAATCAGACCACCGATAATGCCGCCGACAATTGGCACCACCCAGAAGAACCACAGTTGTTCTAATGCCCAGCCGCCCTGGAA  >  NC_000913/914896‑915462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑