Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913449,4610CA37.2% 46.1 / 24.3 43G394V (GGC→GTC) cyoBcytochrome bo3 subunit 1
Reads supporting (aligned to +/- strand):  ref base C (19/8);  new base A (0/16);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 3.13e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAAAGAAGTGGTGCAGCCAAACGATGAACGACAGCACGGTGATACAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACC  >  NC_000913/449164‑449756
                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       
gtgtGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGCTGATCACGTTATGGAAGTGCGCCATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGccc                                                                                                                                                                                                                                                                                                       >  1:211378/1‑300 (MQ=255)
gtgtGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGccc                                                                                                                                                                                                                                                                                                       >  1:67154/1‑300 (MQ=255)
      aaCTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCgag                                                                                                                                                                                                                                                                                                 <  1:426606/300‑1 (MQ=255)
             ggTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGa                                                                                                                                                                                                                                                                                          >  1:842504/1‑300 (MQ=255)
                      gctgGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAAc                                                                                                                                                                                                                                                                                 <  1:385123/300‑1 (MQ=255)
                                    cgGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAATGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCTTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACACTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAATGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAATCCGCGCCCCGTACGACCAGCAGTGTGAAGGGCACCGCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGc                                                                                                                                                                                                                                                                   <  2:481904/300‑1 (MQ=255)
                                    cgGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACTTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCACAACGAAGTCCGCGCCCTGTACGGCCAGCAGCGTGACGGGTATTCCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGc                                                                                                                                                                                                                                                                   <  2:794714/300‑1 (MQ=255)
                                           tGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAg                                                                                                                                                                                                                                                            >  1:429911/1‑300 (MQ=255)
                                               cATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATg                                                                                                                                                                                                                                                        >  1:513756/1‑300 (MQ=255)
                                                     gCCCAGCGCATACAGTTGCATAGAGGCAACTAAGAAGTCGATGAGCCAGAATCAGAACGCGCGTTTACCCCAGGTTTTATTCAGTTTGAAACCGAACGCTTTAGGCCACCATTAGTTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAATGCGCAATCAGGAACAGGCTGTTATGCAGAACAAAGTCCGCGCCCGTTACGGCCAGCAGCGTGAAAGGTACTGCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAacac                                                                                                                                                                                                                                                  <  2:87998/300‑1 (MQ=255)
                                                         aGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATg                                                                                                                                                                                                                                              >  1:113042/1‑300 (MQ=255)
                                                           cgcATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCg                                                                                                                                                                                                                                            >  1:667697/1‑300 (MQ=255)
                                                                 cAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTg                                                                                                                                                                                                                                      >  1:792349/1‑300 (MQ=255)
                                                                     ggCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGGACGCGCTTTTACCCCATGTTTCGTTCATTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGTAAGTGCGCAATCAGGAACAGGCCGTTATGCAGAACGAATTCCGCGCCCTTTACGGCCACCAGCACGACGGGTATGGCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATAc                                                                                                                                                                                                                                  <  2:353247/300‑1 (MQ=255)
                                                                     ggCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGGCATTCCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATAc                                                                                                                                                                                                                                  <  2:549688/300‑1 (MQ=255)
                                                                       cATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGNAGTGCGCAATCAGGAACAGGCTGTTANGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGGCATCCCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACAt                                                                                                                                                                                                                                <  2:596178/300‑1 (MQ=255)
                                                                                                atCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTc                                                                                                                                                                                                       >  1:476699/1‑300 (MQ=255)
                                                                                                 tCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGACCGCTTTAGGCCACCAGTAGGTCATCCATGCGAAGCAGCCGAAGACCACGCCGCCGATGCTCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACAGCCAGCAGCACGCCAGTCATCCCGCCCACAGAGAAGGTGACGATGAAACCGATGGTCCACAGCCTCGCAGCATGGAACACGCTGCGGACCTGCTCCATGGTGAATAGCCAGTTGAAGATCTTCa                                                                                                                                                                                                      >  1:777550/1‑300 (MQ=255)
                                                                                                    agaaccagaacGCGCGTTTACCCCATGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGTTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGGCACCCCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCAccc                                                                                                                                                                                                   <  2:158575/300‑1 (MQ=255)
                                                                                                    agaaccagaacGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCAccc                                                                                                                                                                                                   <  1:898461/300‑1 (MQ=255)
                                                                                                                               tttCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGANGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAATCCGCGCCCGTTACGTCCAGCAGCATGCCAGCCACCCCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTgtgg                                                                                                                                                                        <  2:656794/300‑1 (MQ=255)
                                                                                                                                           tGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGACGGGCACCCCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAaaga                                                                                                                                                            <  2:470904/300‑1 (MQ=255)
                                                                                                                                                     cGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATTAAGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGACGGTCCACAGCATCGCAGAATGGAACACGATGTGGCCCTGACACATGGTGAACAACCAGTTGAAGGTCTTCAACAAGGTCGGGGTGGCGATAGTCATTGTGGTGATACCAAAGAAGGCGTGTAc                                                                                                                                                  >  2:557596/1‑300 (MQ=255)
                                                                                                                                                       cTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGt                                                                                                                                                >  1:358514/1‑300 (MQ=255)
                                                                                                                                                                 ccaGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGc                                                                                                                                      >  1:59083/1‑300 (MQ=255)
                                                                                                                                                                 ccaGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCAGGTCGGGATGGCGCTAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGc                                                                                                                                      >  1:157711/1‑300 (MQ=255)
                                                                                                                                                                  caGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCa                                                                                                                                     >  1:425470/1‑300 (MQ=255)
                                                                                                                                                                   aGTAGGTCATCCCTGCGAGGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACATGCTGTTATGCATACCGAAGTCCGCGCCCGGTACGTCCAGCATCACGCCAGCCATCCCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCAc                                                                                                                                    <  2:795350/300‑1 (MQ=255)
                                                                                                                                                                               cTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCAAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAaaga                                                                                                                        >  1:341830/1‑300 (MQ=255)
                                                                                                                                                                                                 ccACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATNCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAAAGAAGTGGTGCAGCCAAACGa                                                                                                      <  2:650954/300‑1 (MQ=255)
                                                                                                                                                                                                 ccACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATNCAGAACAAAATCCACACCCTGTACTACCACCAGCACGCCAGCCACCCCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAAAGAAGTGGTGCAGCCAAACGa                                                                                                      <  2:650966/300‑1 (MQ=255)
                                                                                                                                                                                                         ccgATGATCACTTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCTCGCCCGTTACGACCAGCATCACGCCAGGCATCCCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAAAGAAGTGGTGCAGCCAAACGATGAACGAc                                                                                              <  2:692360/300‑1 (MQ=255)
                                                                                                                                                                                                                     ttATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAAAGAAGTGGTGCAGCCAAACGATGAACGACAGCACGGTGATa                                                                                  >  1:186954/1‑300 (MQ=255)
                                                                                                                                                                                                                      tctGGAAGTGCTCACTCATGAACAGGCTGTCGTGCACACCGCACTCCCCCCCCAGTACTGCCACCATCACTCCAGACACCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAAAGAAGTGGTGCAGCCAAACGATGAACGACAGCACGGTGATAc                                                                                 <  2:395330/298‑1 (MQ=255)
                                                                                                                                                                                                                          gAAGTGCGCAATCAGAAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGACCACCGAGAAGTTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAAAGAAGTGGTGCAGCCAAACGATGAACGACAGCACGGTGATACAGAc                                                                             <  2:26146/300‑1 (MQ=255)
                                                                                                                                                                                                                                    aTCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAAAGAAGTGGTGCAGCCAAACGATGAACGACAGCACGGTGATACAGACGGTTGCCCAt                                                                   >  1:538121/1‑300 (MQ=255)
                                                                                                                                                                                                                                       aGGAACAGGCTGTTATGCAGAACGAAATCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCACCCCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAAAGAAGTGGTGCAGCCAAACGATGAACGACAGCACGGTGATACAGACGGTTGCCCATAcc                                                                <  2:421455/300‑1 (MQ=255)
                                                                                                                                                                                                                                                  gTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAAAGAAGTGGTGCAGCCAAACGATGAACGACAGCACGGTGATACAGACGGTTGCCCATACCAGCGAGGtata                                                     >  1:253003/1‑300 (MQ=255)
                                                                                                                                                                                                                                                              taaGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGCCATCCCGACCACCTAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAAAGAAGTGGTGCAGCCAAACGATGAACGACAGCACGGTGATACAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACg                                         <  2:675641/299‑1 (MQ=255)
                                                                                                                                                                                                                                                              gAAGTCCGCGCCCGGTACTGCCAGCATCACGCCAGTCATCCCGCCCACCTAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAAAGAAGTGGTGCAGCCAAACGATGAACGACAGCACGGTGATACAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACg                                         <  2:230621/300‑1 (MQ=255)
                                                                                                                                                                                                                                                              gAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGACCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAAAGAAGTGGTGCAGCCAAACGATGAACGACAGCACGGTGATACAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACg                                         <  2:676662/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                            gTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAAAGAAGTGGTGCAGCCAAACGATGAACGACAGCACGGTGATACAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAgg                           >  1:264522/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                            gTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGGAAAGAAGGGGTGCAGCCAAACGATGAACGACAGCACGGTGATACAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAgg                           <  1:438418/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                ggCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAAAGAAGTGGTGCAGCCAAACGATGAACGACAGCACGGTGATACAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACGTTTAAGCGAGAAGGTTGc                       >  1:464510/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                     tccGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGTTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAAAGAAGTGGTGCAGCCAAACGATGAACGACAGCACGGTGATACAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACATTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACAcc  <  2:285409/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                       
GTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGGTTTCGTTCAGTTTGAAACCGAACGCTTTAGGCCACCAGTAGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCATCCCGCCCACCGAGAAGGTGACGATAAAACCGATGGTCCACAGCATCGCAGAATGGAACACGATGCGGCCCTGATACATGGTGAACAGCCAGTTGAAGATCTTCACCCCGGTCGGGATGGCGATAATCATTGTGGTGATACCAAAGAAGGCGTTTACGTTCGCGCCCGCACCCATCGTAAAGAAGTGGTGCAGCCAAACGATGAACGACAGCACGGTGATACAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACC  >  NC_000913/449164‑449756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑