breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
MC JC 260,173 Δ13,782 bp IS5‑mediated proB[ykfC] 19 genes
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 635,355 G→A R8* (CGA→TGA) 
N405N (AAC→AAT
ybdM ←
ybdN ←
ParB‑like nuclease domain‑containing protein YbdM
putative PAPS reductase/DUF3440 domain‑containing protein YbdN
RA 696,582 C→T noncoding (35/75 nt) glnV ← tRNA‑Gln
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,296,375 A→G F583L (TTC→CTC)  adhE ← fused acetaldehyde‑CoA dehydrogenase and iron‑dependent alcohol dehydrogenasealdehyde/alcohol dehydrogenase AdhE
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,705,108 G→A G54R (GGG→AGG)  cnu → H‑NS‑ and StpA‑binding protein
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
JC JC 3,890,570 IS5 (–) +4 bp coding (335‑338/1248 nt) tnaB → tryptophan:H(+) symporter TnaB
RA 4,132,697 G→A E28K (GAG→AAG)  metF → 5,10‑methylenetetrahydrofolate reductase
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,569,394 T→C intergenic (‑667/+768) ytiE ← / ← yjiR protein YtiE/fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1978463 1979327 865 9 [8] [8] 9 insB5–insA5 insB5,insA5
* * ÷ NC_000913 4035327–4035733 4036872–4035735 3–1546 10 [8] [8] 10 [rrsA] [rrsA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 151626 =20 (0.350)14 (0.250) 13/486 2.5 41.2% coding (606/606 nt) yadL putative fimbrial protein YadL
?NC_000913 273955 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 258675NA (NA)10 (0.180) 10/486 3.0 20.4% noncoding (1/768 nt) IS1 repeat region
?NC_000913 362204 = 39 (0.690)coding (976/1254 nt) lacY lactose permease
* ? NC_000913 = 275149NA (NA)11 (0.190) 11/486 2.8 36.7% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 1196827 = 19 (0.330)intergenic (+45/+40) C0293/ymfD small regulatory RNA C0293/putative SAM‑dependent methyltransferase YmfD
* ? NC_000913 = 78168840 (0.700)8 (0.180) 8/378 2.8 22.8% intergenic (+36/‑397) lysQ/nadA tRNA‑Lys/quinolinate synthase
?NC_000913 781845 = 23 (0.520)intergenic (+193/‑240) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 = 119683019 (0.330)12 (0.210) 12/486 2.6 38.7% intergenic (+48/+37) C0293/ymfD small regulatory RNA C0293/putative SAM‑dependent methyltransferase YmfD
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 120780533 (0.580)21 (0.400) 21/454 1.4 47.3% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 16 (0.300)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 12994975 (0.090)34 (0.600) 30/482 0.9 87.3% intergenic (+252/‑70) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 = 1300694 NA (NA)intergenic (+147/‑488) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1299499 =NA (NA)34 (0.630) 31/462 0.8 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 1300694 = 0 (0.000)intergenic (+147/‑488) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1743496 =27 (0.470)19 (0.330) 19/486 1.7 41.3% coding (40/1374 nt) mdtK multidrug efflux pump MdtK
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 174349927 (0.470)14 (0.250) 14/486 2.3 34.2% coding (43/1374 nt) mdtK multidrug efflux pump MdtK
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 1978503 =NA (NA)46 (0.810) 45/486 0.3 78.0% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 2176330 13 (0.230)intergenic (‑9/+20) gatZ/gatY putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone/tagatose‑1,6‑bisphosphate aldolase 2
* ? NC_000913 = 1979270NA (NA)45 (0.790) 41/486 0.4 75.0% noncoding (1/768 nt) IS1 repeat region
?NC_000913 2176322 = 15 (0.260)intergenic (‑1/+28) gatZ/gatY putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone/tagatose‑1,6‑bisphosphate aldolase 2
* ? NC_000913 2098309 =26 (0.460)12 (0.210) 10/482 2.9 31.6% intergenic (‑8/+138) wzzB/ugd O‑antigen co‑polymerase/UDP‑glucose 6‑dehydrogenase
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 209831226 (0.460)20 (0.350) 19/486 1.7 43.5% intergenic (‑11/+135) wzzB/ugd O‑antigen co‑polymerase/UDP‑glucose 6‑dehydrogenase
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 2288919 =NA (NA)10 (0.180) 10/486 3.0 22.2% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 2484281 35 (0.620)coding (527/615 nt) evgA DNA‑binding transcriptional activator EvgA
* ? NC_000913 = 2290113NA (NA)13 (0.230) 13/486 2.5 43.3% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 2469405 = 17 (0.300)coding (275/1332 nt) yfdI serotype‑specific glucosyl transferase YfdI
* ? NC_000913 = 2290113NA (NA)11 (0.190) 10/486 3.0 25.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3206574 33 (0.580)coding (112/912 nt) ttdA L(+)‑tartrate dehydratase subunit alpha
* ? NC_000913 = 255869838 (0.670)14 (0.250) 14/470 2.2 23.4% intergenic (‑19/‑160) eutB/intZ ethanolamine ammonia‑lyase subunit alpha/putative phage integrase IntZ
?NC_000913 2565489 = 55 (1.000)coding (1396/1404 nt) eutA ethanolamine ammonia‑lyase reactivase EutA
* ? NC_000913 = 311059112 (0.210)16 (0.280) 16/486 2.1 57.1% coding (536/537 nt) yghD putative type II secretion system M‑type protein YghD
?NC_000913 3130146 = NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 3186096 =NA (NA)13 (0.230) 12/486 2.6 27.1% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 3367745 = 35 (0.620)intergenic (+103/‑82) yhcF/yhcG DUF1120 domain‑containing protein YhcF/DUF1016 domain‑containing protein YhcG
* ? NC_000913 = 3187426NA (NA)20 (0.350) 19/486 1.7 35.7% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 3367749 36 (0.630)intergenic (+107/‑78) yhcF/yhcG DUF1120 domain‑containing protein YhcF/DUF1016 domain‑containing protein YhcG
* ? NC_000913 3652036 =NA (NA)12 (0.210) 11/486 2.8 22.2% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 3843042 42 (0.740)coding (748/1335 nt) adeQ adenine transporter
* ? NC_000913 4117984 =34 (0.600)11 (0.190) 11/482 2.8 24.4% coding (107/846 nt) glpF glycerol facilitator
?NC_000913 4507459 = NA (NA)noncoding (1221/1221 nt) IS30 repeat region
* ? NC_000913 4163082 =34 (0.600)29 (0.510) 28/484 1.0 46.0% coding (189/1101 nt) trmA tRNA m(5)U54 methyltransferase
?NC_000913 = 4499511 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 = 416308634 (0.600)19 (0.340) 19/484 1.7 35.9% coding (185/1101 nt) trmA tRNA m(5)U54 methyltransferase
?NC_000913 4498181 = NA (NA)noncoding (1/1331 nt) IS2 repeat region