Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,177,286	C→T	intergenic (+158/‑72)	tufB → / → secE	translation elongation factor Tu 2/Sec translocon subunit SecE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,177,286	0	C	T	100.0%	331.4 / NA	89	intergenic (+158/‑72)	tufB/secE	translation elongation factor Tu 2/Sec translocon subunit SecE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/44);  total (45/44)

ATGGTAATGCCGGGCGACAACATCAAAATGGTTGTTACCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGACAAAAGGTAAAGCTACCGTTGCTTTTGCC  >  NC_000913/4176991‑4177573                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                aTGGTAATGCCGGGCGACAACATCAAAATGGTTGTTACCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGt                                                                                                                                                                                                                                                                                             <  1:404634/300‑1 (MQ=255)  tGGTAATGCCGGGCGACAACATCAAAATGGTTGTTACCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTCGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTNCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGtt                                                                                                                                                                                                                                                                                            >  2:786763/1‑300 (MQ=255)   ggTAATGCCGGGCGACAACATCAAAATGGTTGTTACCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGTGTATAATCCGAAAAGCGAGTATGCGttt                                                                                                                                                                                                                                                                                           >  2:94470/1‑300 (MQ=255)                     caTCAAAATGGTTGTTACCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTcgc                                                                                                                                                                                                                                                                         >  1:422716/1‑300 (MQ=255)                       tCAAAATGGTTGTTACCCTGATCCACCCGATCGCGATGGACGATGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGa                                                                                                                                                                                                                                                                       <  1:414354/300‑1 (MQ=255)                       ctgcgagatgtgtataagagacagaCCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTCCCAGAACCCGGCTCCTTAGTGGTTTTCTTTGTCATCACCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGa                                                                                                                                                                                                                                                                       >  1:872922/25‑300 (MQ=37)                               gttgttACCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCgggg                                                                                                                                                                                                                                                               >  1:425169/1‑300 (MQ=255)                                ttgttACCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGTTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGt                                                                                                                                                                                                                                                              >  2:815370/1‑300 (MQ=255)                                ttgttACCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAGGCTAATATGCGTTTCGATTTGGTTTGCCTTGCGATCGCGGGGt                                                                                                                                                                                                                                                              >  2:390813/1‑300 (MQ=255)                                ttgttACCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGt                                                                                                                                                                                                                                                              >  2:390537/1‑300 (MQ=255)                                ttgttACCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGt                                                                                                                                                                                                                                                              >  1:167601/1‑300 (MQ=255)                                     aCCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATTATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAACGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGaaaa                                                                                                                                                                                                                                                         >  2:136500/1‑300 (MQ=255)                                     aCCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGaaaa                                                                                                                                                                                                                                                         >  2:155883/1‑300 (MQ=255)                                                              acgacgGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATCTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTATGTTGAGGGTGTATAATCCGAAAAGCTAATATGCGTTTAGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAggt                                                                                                                                                                                                                                >  2:73143/1‑300 (MQ=255)                                                                   ggTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGCCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAggttggtt                                                                                                                                                                                                                           <  2:774482/300‑1 (MQ=255)                                                                         cGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGTGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTGATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAg                                                                                                                                                                                                                     <  2:539759/300‑1 (MQ=255)                                                                         cGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTTAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAg                                                                                                                                                                                                                     <  2:853574/300‑1 (MQ=255)                                                                         cGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTATGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAg                                                                                                                                                                                                                     <  2:681188/300‑1 (MQ=255)                                                                                          aGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTc                                                                                                                                                                                                    <  1:384282/300‑1 (MQ=255)                                                                                               gcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAANATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGa                                                                                                                                                                                               <  2:660403/300‑1 (MQ=255)                                                                                               gcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGa                                                                                                                                                                                               >  1:438647/1‑300 (MQ=255)                                                                                               gcCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGa                                                                                                                                                                                               >  2:49170/1‑300 (MQ=255)                                                                                                     ccGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCggg                                                                                                                                                                                         <  1:72937/300‑1 (MQ=255)                                                                                                                ggCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGa                                                                                                                                                                              <  1:709470/300‑1 (MQ=255)                                                                                                                  cGTTGTNGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACNAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAg                                                                                                                                                                            <  2:741630/300‑1 (MQ=255)                                                                                                                  cGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCNTCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAg                                                                                                                                                                            <  1:627327/300‑1 (MQ=255)                                                                                                                  cGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTTAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAg                                                                                                                                                                            <  1:121218/300‑1 (MQ=255)                                                                                                                  cGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAg                                                                                                                                                                            <  1:223524/300‑1 (MQ=255)                                                                                                                  cGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGGACCAGAACCTGGCTCATCAGTGATTTTCTTTGTAATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAg                                                                                                                                                                            <  1:55077/300‑1 (MQ=255)                                                                                                                  cGTTGTAGCAAAAGCTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGTGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTTAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAg                                                                                                                                                                            <  2:302692/300‑1 (MQ=255)                                                                                                                       tAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATg                                                                                                                                                                       <  2:316708/300‑1 (MQ=255)                                                                                                                                      gCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTtgtg                                                                                                                                                        >  2:423838/1‑300 (MQ=255)                                                                                                                                       cTAATTNCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCNCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTgtgg                                                                                                                                                       <  2:746035/300‑1 (MQ=255)                                                                                                                                         aaTTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTgtggtg                                                                                                                                                     <  2:276690/300‑1 (MQ=255)                                                                                                                                          aTTGCCGATAACATTTGACGCAATGCGCACTAAAAGTGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGtggtgg                                                                                                                                                    <  1:877564/300‑1 (MQ=255)                                                                                                                                                  tAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTc                                                                                                                                            <  1:466246/300‑1 (MQ=255)                                                                                                                                                     cATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCtg                                                                                                                                         >  1:127111/1‑300 (MQ=255)                                                                                                                                                       tttGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCtggt                                                                                                                                       <  1:130622/300‑1 (MQ=255)                                                                                                                                                         tGACGCAATGCGCACTAAAAGTGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCtggtgg                                                                                                                                     <  1:873334/300‑1 (MQ=255)                                                                                                                                                                     cACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAANCTAATATGCGTTTCGATTTGGTTTGCNTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACGTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGCTGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCa                                                                                                                         >  2:591836/1‑300 (MQ=255)                                                                                                                                                                       cTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAAc                                                                                                                       >  2:566136/1‑300 (MQ=255)                                                                                                                                                                                     tttGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGNGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGa                                                                                                         >  2:795186/1‑300 (MQ=255)                                                                                                                                                                                                   tGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTTAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGc                                                                                           <  1:71771/300‑1 (MQ=255)                                                                                                                                                                                                     cACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTTAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCtg                                                                                         <  1:576552/300‑1 (MQ=255)                                                                                                                                                                                                     cACGCTTTCGTACCAGAACCTGGCTCAGCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCtg                                                                                         <  1:702127/300‑1 (MQ=255)                                                                                                                                                                                                       cGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCtgcg                                                                                       <  1:883279/300‑1 (MQ=255)                                                                                                                                                                                                       cGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCtgcg                                                                                       <  2:573745/300‑1 (MQ=255)                                                                                                                                                                                                         cTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGCTCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCNTGGTGGCGATTGTCGGCAACTATCTTTCTCGCGACATTATGCTGCCGCtgcgtg                                                                                     >  2:658048/1‑300 (MQ=255)                                                                                                                                                                                                         cTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCtgcgtg                                                                                     >  2:418219/1‑300 (MQ=255)                                                                                                                                                                                                         cTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGACGATTGTCGGCAACTATCTTTATCGCGACATCATGCTGCCGCtgcgtg                                                                                     >  2:136604/1‑300 (MQ=255)                                                                                                                                                                                                            tCGTACCAGAACTTGGATCATCATTGATGTTCGTTGTCATGATCATTGCTGAGACAGGCTCGGTTGAGTGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTCGCCTCGCGATCGCGGGGTGAAAATGTTTGTATAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGTAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGTTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTgcgc                                                                                  <  1:136903/300‑1 (MQ=255)                                                                                                                                                                                                               tACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTgg                                                                               >  1:728651/1‑300 (MQ=255)                                                                                                                                                                                                                    gcaCCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCgta                                                                          >  1:712927/3‑300 (MQ=255)                                                                                                                                                                                                                    gAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCATGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCgta                                                                          >  1:709289/1‑300 (MQ=255)                                                                                                                                                                                                                         tGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGNAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAAt                                                                     >  2:210060/1‑300 (MQ=255)                                                                                                                                                                                                                           gCTCATCAGTGATTTTCTTTTTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACNTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGTTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTc                                                                   <  2:220650/300‑1 (MQ=255)                                                                                                                                                                                                                           gCTCATCAGTGATTTTCTTTGTCATAATCATTGCTTAGACAGGCTCTGCTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTNATGAGTGCGAATACCGAAGCTCAAGGNAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTc                                                                   <  2:596126/300‑1 (MQ=255)                                                                                                                                                                                                                              catcaGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCGTTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGa                                                                >  2:548861/1‑300 (MQ=255)                                                                                                                                                                                                                                         ttCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCg                                                     <  1:421970/300‑1 (MQ=255)                                                                                                                                                                                                                                          tCTTTGTCATGATCATTGCTTAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAACTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGTTTGGTTTATGAGTGCGAATACCGAAGCGCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCgg                                                    <  1:704350/300‑1 (MQ=255)                                                                                                                                                                                                                                          tCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCgg                                                    <  1:703313/300‑1 (MQ=255)                                                                                                                                                                                                                                               gTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGggtggt                                               >  2:563339/1‑300 (MQ=255)                                                                                                                                                                                                                                                 cATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGNTGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCNTAGTAATTCTGATTGCTGCAGCGGGTGgtgt                                             <  2:654580/300‑1 (MQ=255)                                                                                                                                                                                                                                                     aTCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTcgcg                                         >  2:385124/1‑300 (MQ=255)                                                                                                                                                                                                                                                      tCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCgcgc                                        <  1:900609/300‑1 (MQ=255)                                                                                                                                                                                                                                                      gtgtataagagaCAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCgcgc                                        <  2:161051/292‑1 (MQ=255)                                                                                                                                                                                                                                                         ttGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGt                                     >  1:289203/1‑300 (MQ=255)                                                                                                                                                                                                                                                           gCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTa                                   >  2:684070/1‑300 (MQ=255)                                                                                                                                                                                                                                                              tagaCAGGCTCTGTTGAGGGCGTATAATCCTAAAAGCTAATATGCGTTTCGATGTGGTTTGCCTCGCGATCGCTGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACg                                <  1:274884/299‑1 (MQ=255)                                                                                                                                                                                                                                                              gggaCAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACg                                <  2:303458/298‑1 (MQ=255)                                                                                                                                                                                                                                                                gaCAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGTTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGGAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGAc                              <  2:405604/300‑1 (MQ=255)                                                                                                                                                                                                                                                                gaCAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGAc                              >  2:422438/1‑300 (MQ=255)                                                                                                                                                                                                                                                                gaCAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGAc                              >  1:16069/1‑300 (MQ=255)                                                                                                                                                                                                                                                                gaCAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTGCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGAc                              <  2:420730/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     gCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCTCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTTGCGCTGTTAACGACAAAAg                         >  2:124110/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     gCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCgcgcctgtctcttatacac                         >  1:161715/1‑285 (MQ=255)                                                                                                                                                                                                                                                                     gCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCATGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGACAAAAg                         >  2:172469/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGGTCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGACAAAAGGTaaa                    >  2:701166/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGACAAAAGGTaaa                    >  1:564993/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGAAAAAAGGTaaa                    >  2:267856/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGANTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGACAAAAGGTaaa                    >  1:660184/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTAGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTACAGCGGGTGGTGTCGCGCTGTTAACGACAAAAGGTaaa                    >  2:859745/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gTTGAGGGCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGCAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGACAAAAGGTaaa                    >  1:849844/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            tGAGGGCGTATAATCCGAAAATCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCTGGTTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCTAAGCTCAAGGAAGCTGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGTCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGACAAAAGGTAAAGc                  <  2:313136/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 gCGTATAATCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACNATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGACAAAAGGTAAAGCTACCg             >  2:789326/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  cGTATAATCCGAAAAGCTAATATGTGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCATGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCAGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGACAAAAGGTAAAGCTACCGt            >  2:44775/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       aaTCCGAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGACAAAAGGTAAAGCTACCGTTGCtt       >  1:588386/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            gAAAAGCTAATATGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGACAAAAGGTAAAGCTACCGTTGCTTTTGcc  <  1:465377/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            gAAAAGCTAATATGCGTTTCGATTTGGTTTGCCGCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGACAAAAGGTAAAGCTACCGTTGCTTTTGcc  <  1:465279/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                ATGGTAATGCCGGGCGACAACATCAAAATGGTTGTTACCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGTGAAAATGTTTGTAGAAAACTTCTGACAGGTTGGTTTATGAGTGCGAATACCGAAGCTCAAGGAAGCGGGCGCGGCCTGGAAGCGATGAAGTGGGTCGTTGTGGTGGCATTGCTCCTGGTGGCGATTGTCGGCAACTATCTTTATCGCGACATTATGCTGCCGCTGCGTGCGCTGGCCGTAGTAATTCTGATTGCTGCAGCGGGTGGTGTCGCGCTGTTAACGACAAAAGGTAAAGCTACCGTTGCTTTTGCC  >  NC_000913/4176991‑4177573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑