Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,122,884 C→T T152T (ACG→ACA)  ftsN ← cell division protein FtsN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,122,8840CT97.6% 294.4 / ‑6.8 83T152T (ACG→ACA) ftsNcell division protein FtsN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (43/38);  minor base G (0/2);  total (43/40)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACCATCCAGCGGCGTTCGTCTTTTTTCTCCGCCGTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGT  >  NC_000913/4122589‑4123177
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                     
gCACCATCCATCGGCGTTCGTCTTTTTTCTCCGCCGTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTATGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCGGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGttt                                                                                                                                                                                                                                                                                                   <  2:370448/300‑1 (MQ=255)
gCACCATCCAGCTGCGTTCGTCTTTTTTCTCCGCCGTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGttt                                                                                                                                                                                                                                                                                                   <  1:228563/300‑1 (MQ=255)
gCACCATCCAGCGGCGTTCGTCTTTTTTCTCCGCCGTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGttt                                                                                                                                                                                                                                                                                                   >  2:555378/1‑300 (MQ=255)
gCACCATCCAGCGGCGTTCGTCTTTTTTCTCCGCCGTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGATGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGttt                                                                                                                                                                                                                                                                                                   >  1:479974/1‑300 (MQ=255)
  gccATCCAACGGCGTTCATCTTTTTTCGCCGCCGTCGGTTTTTGCGCGTCAGCGGCACGAAAAACTGGCGCGGCGTTCTGCGACTTCGACTGCGCATTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACNGCTGCTGAGAAGAAGCCGGTTTAGGCTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCATTCGTACGCTACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGc                                                                                                                                                                                                                                                                                                 <  2:228514/299‑1 (MQ=255)
     gtCCAGCGGCGTTCGTCTTTTTTCTCCGCCGTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTNGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTNGCGACGTACGCGTCTGCTGCTGCAAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGa                                                                                                                                                                                                                                                                                              >  2:717534/2‑300 (MQ=255)
     aTCCAGCGGCGTTCGTCTTTTTTCTCCGCCGTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGa                                                                                                                                                                                                                                                                                              >  1:740483/1‑300 (MQ=255)
     aTCCAGCGGCGTTCGTCTTTTTTCTCCGCAGTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGa                                                                                                                                                                                                                                                                                              >  1:840248/1‑300 (MQ=255)
             gcgTTCGTCTTTTTTCTCCGCCGTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTc                                                                                                                                                                                                                                                                                      <  1:688075/300‑1 (MQ=255)
                ttCGTCTTTTTTCTCCGCCGTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCgg                                                                                                                                                                                                                                                                                   <  1:94864/300‑1 (MQ=255)
                ttCGTCTTTTTTCTCCGCCGTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGANCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCgg                                                                                                                                                                                                                                                                                   <  1:617948/300‑1 (MQ=255)
                  cGTCTTTTTTCTCCGCCGTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCg                                                                                                                                                                                                                                                                                 <  1:462202/300‑1 (MQ=255)
                              ccgccgTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTcc                                                                                                                                                                                                                                                                     <  1:537900/300‑1 (MQ=255)
                                   ttCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGa                                                                                                                                                                                                                                                                <  2:172909/299‑1 (MQ=255)
                                            ggCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTTTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAAcc                                                                                                                                                                                                                                                       <  1:577252/300‑1 (MQ=255)
                                              cgcgTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTNGCGCCTGTACCGGTGCGGCTTGCGACNTCCGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCAAGGAACTTCAACCAg                                                                                                                                                                                                                                                     >  2:602631/1‑300 (MQ=255)
                                                         cACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGgct                                                                                                                                                                                                                                          <  1:797557/300‑1 (MQ=255)
                                                           cGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGgctgc                                                                                                                                                                                                                                        <  1:108147/300‑1 (MQ=255)
                                                                    ggCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGAAGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCata                                                                                                                                                                                                                               >  1:329389/1‑300 (MQ=255)
                                                                      cgcAGCCTGCTGCGGCTTCGACTGCGCATTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACTGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATc                                                                                                                                                                                                                             <  2:170521/300‑1 (MQ=255)
                                                                            ttGCTGCGGCTTCGACTGCGCAGTCGCGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTACTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTAAACCAGCTGCGTTGGCTGCTGGCGCATATTAGCCTg                                                                                                                                                                                                                       >  2:104678/1‑300 (MQ=255)
                                                                               ctgcGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCAt                                                                                                                                                                                                                    >  2:763080/1‑300 (MQ=255)
                                                                               ctgcGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTGGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCAt                                                                                                                                                                                                                    <  1:828773/300‑1 (MQ=255)
                                                                               ctgcGGCTTCGACTGCGCAGTCGTGTGCGCAGGAATTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTTCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGTATATTAGCCTGCAt                                                                                                                                                                                                                    <  1:828772/300‑1 (MQ=255)
                                                                                tgcGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTTCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTCCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCAtt                                                                                                                                                                                                                   <  2:703317/300‑1 (MQ=255)
                                                                                   ggCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGt                                                                                                                                                                                                                >  1:557772/1‑300 (MQ=255)
                                                                                   ggCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTAAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGt                                                                                                                                                                                                                >  2:179400/1‑300 (MQ=255)
                                                                                    gCTTCGNCTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTNCTGAGAAGAAGCCGGGTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTTACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGtt                                                                                                                                                                                                               <  2:743282/300‑1 (MQ=255)
                                                                                          aCTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCaagaa                                                                                                                                                                                                         <  1:727331/300‑1 (MQ=255)
                                                                                                  gTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGCCGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACgt                                                                                                                                                                                                 >  2:199055/1‑300 (MQ=255)
                                                                                                  gTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTACGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACgt                                                                                                                                                                                                 <  1:506199/300‑1 (MQ=255)
                                                                                                  gTCGTGTGCGCAGGAGTTGGCAGCAGATCCTTGTACTGCTGCTGAGAAGGAGCCGGTTTAGACTGTCGCGGTGGCGCCTTTACCGGTGCGGCTTTCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACGGATTAGCTAGGCGTTGCTGTTCCACTAATTGCTGCGCCTGACGTTTGCGCTGTAGGGTGTGCTGACGCTGGGCCGTCGTCTGTTCAGTCCACGGAACGTCAACCAGCGGCATTGGCTGCGGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACgt                                                                                                                                                                                                 <  1:500004/300‑1 (MQ=255)
                                                                                                                 gttcgcagcaGATCCTTGGGCGGCTGCTGAGAAGAAGCCGGTTTGGACTGGTGCGGTTGCGTCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGGTCAGGCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCGTTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTGGGGCTGGTGTCAGt                                                                                                                                                                                  <  1:364812/296‑1 (MQ=255)
                                                                                                                 gTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGt                                                                                                                                                                                  >  1:747184/1‑300 (MQ=255)
                                                                                                                 gTTTGCAGCAGATCCTGGTACGGCTGCGGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGt                                                                                                                                                                                  <  1:517947/300‑1 (MQ=255)
                                                                                                                             tCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGtt                                                                                                                                                                      >  1:471817/1‑300 (MQ=255)
                                                                                                                                   tACGACTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCGTGCGACGTACGCGTCTGCGGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAGTTGCTGCGCCTNACGTTGGCGCTGTAGTGTTTGCTGACNCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGCTGCTCCGGCGTTttcact                                                                                                                                                                <  2:607613/300‑1 (MQ=255)
                                                                                                                                     cGGCTGCTGAGAAGAAGCCGGTTTAGACTGACGCGGTTGCGCCTGTACCGATGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTACTGAGCCAGGCGTTGCTGTTCAGATAATTGCTGCGCATGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCATGAATTTGTTCAAGAAGCGGACGTTGTTCTGGTGTCAGTTGCTCCGGCTTTttcactta                                                                                                                                                              >  2:112160/1‑299 (MQ=255)
                                                                                                                                          gctgAGAAGAAGCCGGTTTAGAATGGCGCGGTTGCGCCTGGACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTcaccac                                                                                                                                                         <  2:339525/300‑1 (MQ=255)
                                                                                                                                               gaagaaGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCa                                                                                                                                                    >  1:130429/1‑300 (MQ=255)
                                                                                                                                               gaagaaGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGAAGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCa                                                                                                                                                    >  1:241993/1‑300 (MQ=255)
                                                                                                                                                  gaagCCGGTTTAGACTGTCGCGTTTGCGCCTGTACCGGTGCGGCTTTCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTACTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGaa                                                                                                                                                 <  2:84144/300‑1 (MQ=255)
                                                                                                                                                  gaagCCGGTTTAGACTGGCGCGGTTGCGCCTGTATCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCATGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGaa                                                                                                                                                 >  2:805079/1‑300 (MQ=255)
                                                                                                                                                   aagCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAg                                                                                                                                                <  2:511455/300‑1 (MQ=255)
                                                                                                                                                       cggtttatgacgtggcgGTTGCGCCTGTATCGGTGCGGCTTGCGACGTACGCGTCGGCTGCTGCCAGCTTTCTTCATTCGTACGCTACTGCTGAGCCATGCGTTGCTGTTCCGCTAATTGCTGCACCGGACGTGGGCGCTGTAAGATTTGCTGACGCTGTTCCGGTGTCTGTTCATTCCACGGAACTTCAACCAACTGCGGTGGCTGCTGGCGCATATCAGCCTCCATTTGTTCAAGAATCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTCCACTTCACCACCTGCAGAAGGTTc                                                                                                                                            <  2:366662/286‑1 (MQ=255)
                                                                                                                                                        ggTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGGTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCAACACCGGCAGAAGGTTCt                                                                                                                                           >  2:699616/1‑300 (MQ=255)
                                                                                                                                                             aGACTGTCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAACTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTggg                                                                                                                                      <  2:101114/300‑1 (MQ=255)
                                                                                                                                                             aGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTggg                                                                                                                                      >  1:88863/1‑300 (MQ=255)
                                                                                                                                                                        gTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTc                                                                                                                           >  1:579863/1‑300 (MQ=255)
                                                                                                                                                                         ttGCGCCTGTACCTGTGCTGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCTACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTTTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTcc                                                                                                                          <  2:395740/300‑1 (MQ=255)
                                                                                                                                                                            cgcCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCgg                                                                                                                       >  1:379334/1‑300 (MQ=255)
                                                                                                                                                                              ccTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCTGCt                                                                                                                     <  1:764248/300‑1 (MQ=255)
                                                                                                                                                                              ccTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGNTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCt                                                                                                                     <  1:602316/300‑1 (MQ=255)
                                                                                                                                                                                                     gtattcgTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCGAATTGCCGCGCCTTACGTTGGCGCTGTAGTGTTTGCNTACGCTGTTCCTGCGTCTGTTCATTCCCCTGAACTTCAACCAGCTTCGTTGGCTGCTGGCGCATATCGGCCGGCATTGGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTaagg                                                                                              <  2:776280/295‑3 (MQ=255)
                                                                                                                                                                                                     gTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATg                                                                                              >  2:758179/1‑300 (MQ=255)
                                                                                                                                                                                                      tACGCGTCTGCTGCTGCCAGCTTTGTTCATTCGTACGCGACTGCTGAGCCATGCGTTGCTGTTCCGCTAATTGCTGCGCCTGGCGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACCTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGt                                                                                             <  2:541762/300‑1 (MQ=255)
                                                                                                                                                                                                          cgTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGCGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGCCGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTAACCACCGGCAGAAGNTTCTGTGGGAGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAgcg                                                                                         >  2:661256/1‑300 (MQ=255)
                                                                                                                                                                                                                ctgctgcCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCg                                                                                   <  1:265596/300‑1 (MQ=255)
                                                                                                                                                                                                                      ccAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCTGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGGGCATATCAGCCTGCATTTGTTCAAGANGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTNCCAGCTCTTTAATGTAGCGCCAGCGttcttc                                                                             <  2:634655/300‑1 (MQ=255)
                                                                                                                                                                                                                       cAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTtcttct                                                                            >  1:470321/1‑300 (MQ=255)
                                                                                                                                                                                                                           tttGTTCAGTCGTACGCGACTGCTGAGCCAGGTGTTGCTGTTCCGCTAATTGCTGCGCCTTACGTTGGCGCTTTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGtt                                                                        <  2:369436/300‑1 (MQ=255)
                                                                                                                                                                                                                              gTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTtg                                                                     >  1:134603/1‑300 (MQ=255)
                                                                                                                                                                                                                                   gTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTggtggt                                                                >  1:369384/1‑300 (MQ=255)
                                                                                                                                                                                                                                   gTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTCCCAGCTCTTTACTGTAGCACCAACGTTCTTCTGGTTTTggtggt                                                                >  2:10723/1‑300 (MQ=255)
                                                                                                                                                                                                                                   gTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCAACACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTggtggt                                                                >  2:374985/1‑300 (MQ=255)
                                                                                                                                                                                                                                      gTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCAACGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCAAGCGTTCTTCTGGTTTTGGTGGTAGt                                                             >  2:241495/1‑300 (MQ=255)
                                                                                                                                                                                                                                             aCTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTc                                                      <  1:382378/300‑1 (MQ=255)
                                                                                                                                                                                                                                                         ggCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCt                                          >  2:566120/1‑300 (MQ=255)
                                                                                                                                                                                                                                                          gCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTg                                         >  1:565994/1‑300 (MQ=255)
                                                                                                                                                                                                                                                          gCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATACCCGCCTGCATTTGTTTAAGGAGCTGACGTTGTTCTGGTGTCATTTGCTCCAGCGGTTCCCCCTAAACAAAGGCAGAAAACTCTACGGGCGCCCGCACTCCTGTATGGCAAATTCCCAGCTCTTTCATGTAACGCCCGCGTTGTTCTGGtttcattggtagcacgtccctggtcactttctg                                         >  2:583162/1‑270 (MQ=255)
                                                                                                                                                                                                                                                            gTTGCTGTTCCGCTAATTGCCGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCTGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGc                                       <  2:535578/300‑1 (MQ=255)
                                                                                                                                                                                                                                                             ttGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCCTTTTTTCCAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCt                                      >  2:855851/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                       gCTAATTGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGtc                            >  1:684556/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                            ttGCTGCGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTGTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGa                       >  2:848024/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                             tgctgcGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGAc                      <  1:850094/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                               ctgcGCCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGActc                    <  1:687009/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 gcgcCTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACtctt                  >  2:699190/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                  cgcCTGACGTTGACGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCNCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTATGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACtcttc                 >  2:796111/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                    ccTGACGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTcttctt               >  1:228742/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                        aCGTTGGCGCTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTtctttctt           >  1:771910/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                              gcgcTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATg     >  1:298334/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                              gcgcTGCAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATg     >  2:693855/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                 cTGTAGTGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTAGGACTCTTCTTTCTTGTGATGCGt  >  2:369158/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                     
GCACCATCCAGCGGCGTTCGTCTTTTTTCTCCGCCGTCGGTTTTGGCGCGTCAGCGGCACGAGCAACTGGCGCAGCTTGCTGCGGCTTCGACTGCGCAGTCGTGTGCGCAGGAGTTTGCAGCAGATCCTGGTACGGCTGCTGAGAAGAAGCCGGTTTAGACTGGCGCGGTTGCGCCTGTACCGGTGCGGCTTGCGACGTACGCGTCTGCTGCTGCCAGCTTTGTTCAGTCGTACGCGACTGCTGAGCCAGGCGTTGCTGTTCCGCTAATTGCTGCGCCTGACGTTGGCGCTGTAGCGTTTGCTGACGCTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTGTGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGTAGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGT  >  NC_000913/4122589‑4123177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑