BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,797,240	C→T	P98L (CCA→CTA)	waaL →	O‑antigen ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,797,240	0	C	T	99.5%	683.6 / NA	187	P98L (CCA→CTA)	waaL	O‑antigen ligase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (75/111); total (76/111)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 187 total aligned reads displayed.

AAACATGCTAACATCCTTTAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAATGCTAATAGGGATAGTTAGTGGCGTTGCGATTCTTTATACTAAGAAAAATCATCCTT  >  NC_000913/3796944‑3797533

           cATCCTTTAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATNGTTTGCTTATTGTCACTAATTTTACGNGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACtt                                                                                                                                                                                                                                                                                         <  2:612377/300‑1 (MQ=255)

           cATCCTTTAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACtt                                                                                                                                                                                                                                                                                         >  1:181763/1‑300 (MQ=255)

              ccTTTCAACTTCATTCCTTGACACCTTACCCTCTGCAATCATCCATGATTTTAGCGATACTAACTTATATATTATGTTTTTTTTCAATGGTAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATCTCACTGCTATAGTTTGCTTCTTGTCACTAATTTTCCGTGGCAGACAAGAAAATTACAATATAAAAACCCCTATTCTTCACCTTTCTATATTTTTACTAGGCTTGCTTGATTTACTTTGGTATTTTGCGTTTAAAGTAGATACTTCGCTATTTAGTGCTACTTAcc                                                                                                                                                                                                                                                                                      >  1:146330/1‑300 (MQ=255)

              ccTTTAAACTTCATTCATTTAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTAcc                                                                                                                                                                                                                                                                                      <  1:48919/300‑1 (MQ=255)

              ccTTTAAACTTCATTCATTGAAACCTTACACTCTTAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGNTAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTAcc                                                                                                                                                                                                                                                                                      <  2:775961/300‑1 (MQ=255)

              ccTTTAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTAcc                                                                                                                                                                                                                                                                                      <  2:557509/300‑1 (MQ=255)

                tttAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACGTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCAGAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTGATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCAt                                                                                                                                                                                                                                                                                    <  2:407507/300‑1 (MQ=255)

                  tAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAg                                                                                                                                                                                                                                                                                  <  1:529499/300‑1 (MQ=255)

                   aaaCTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGt                                                                                                                                                                                                                                                                                 <  1:364450/300‑1 (MQ=255)

                        tcattcatTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTAttt                                                                                                                                                                                                                                                                            <  1:859210/300‑1 (MQ=255)

                         cattcattGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTa                                                                                                                                                                                                                                                                           <  1:762161/300‑1 (MQ=255)

                                  aaaCCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGcc                                                                                                                                                                                                                                                                  >  1:344268/1‑300 (MQ=255)

                                    cccTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACCGCTATCGTTTACTTATTGTCCATAATTTTACGTGGCCGACAATACAATTATAATATAAAACACCTTATCCTTCCCCTTTCTATCTTTTTCATCGTCTCGCTTGATTTACTTTGGTATTCTGCGTTTAAAGTAGATAACTCACTATTTCGTGCTACTTAAAATCGTTATTTACATACTGcaaa                                                                                                                                                                                                                                                                >  1:236767/2‑297 (MQ=255)

                                    aCCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAGAAAACCTTATTCTCCCCATTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTCGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATAATGcaaa                                                                                                                                                                                                                                                                >  2:321323/1‑297 (MQ=255)

                                    aCCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATAATGCCaa                                                                                                                                                                                                                                                                >  2:192333/1‑300 (MQ=255)

                                    aCCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCATTTCTATATTTCTAATAGGCTTGCTTGCTTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACTATAGTTATTTAAATACTGCCaa                                                                                                                                                                                                                                                                >  2:76381/1‑300 (MQ=255)

                                    aCCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCAATGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCACCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCaa                                                                                                                                                                                                                                                                >  2:271858/1‑300 (MQ=255)

                                      cTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCaaaa                                                                                                                                                                                                                                                              >  1:797153/1‑300 (MQ=255)

                                       ttACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAat                                                                                                                                                                                                                                                             <  1:495403/300‑1 (MQ=255)

                                            ctctGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAatattta                                                                                                                                                                                                                                                        >  1:114113/1‑300 (MQ=255)

                                                taaaccatcaACGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTGTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAatatttatatt                                                                                                                                                                                                                                                    <  2:529728/295‑1 (MQ=255)

                                                             aTTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTAtt                                                                                                                                                                                                                                       >  1:598113/1‑300 (MQ=255)

                                                             aTTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTGTAAAGTAGATAATTCGCTATTTCGTGCNACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTAtt                                                                                                                                                                                                                                       >  2:656569/1‑300 (MQ=255)

                                                              ttttAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTg                                                                                                                                                                                                                                      <  1:220995/300‑1 (MQ=255)

                                                                     gctaataaCTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAACTATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTCCGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTTTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCtt                                                                                                                                                                                                                               >  1:272402/3‑300 (MQ=255)

                                                                             cTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACactaa                                                                                                                                                                                                                       >  1:401122/1‑300 (MQ=255)

                                                                               tatgtcttatGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTGCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACactaact                                                                                                                                                                                                                     <  2:233367/294‑1 (MQ=255)

                                                                               tatataTTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACactaact                                                                                                                                                                                                                     <  2:420647/300‑1 (MQ=255)

                                                                                   tattatGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAANAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGANTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGcc                                                                                                                                                                                                                 <  2:704041/300‑1 (MQ=255)

                                                                                   tattatGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTNTCTATATTTTTAATAGGCTTGCTTGANTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGcc                                                                                                                                                                                                                 <  2:620426/300‑1 (MQ=255)

                                                                                   tattatGTTTTTTTTCAATGATAATTGCATTCGGCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGcc                                                                                                                                                                                                                 <  1:38717/300‑1 (MQ=255)

                                                                                   tattatGTTTTTTTTCAATGAGAATTGCATGCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTCCCCCTTTCTATATTTTCAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGcc                                                                                                                                                                                                                 <  2:7281/300‑1 (MQ=255)

                                                                                   gattatGTTTTTTTTCAGTGATAATGGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGcc                                                                                                                                                                                                                 <  2:116077/299‑1 (MQ=255)

                                                                                                        tAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTCATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAagaga                                                                                                                                                                                            >  1:559114/1‑300 (MQ=255)

                                                                                                                 tcgtcgATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGtattat                                                                                                                                                                                   <  1:61256/300‑1 (MQ=255)

                                                                                                                   gtcgATAATACTTTCAGTATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATac                                                                                                                                                                                 <  2:346122/300‑1 (MQ=255)

                                                                                                                   gtcgATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATac                                                                                                                                                                                 <  1:114359/300‑1 (MQ=255)

                                                                                                                   gtcgATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCNTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATac                                                                                                                                                                                 <  1:622031/300‑1 (MQ=255)

                                                                                                                   gtcgATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAAACTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATCTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATac                                                                                                                                                                                 >  2:460731/1‑300 (MQ=255)

                                                                                                                     tgataagacgtgcAGCATAAACATAGATAATATCACTGATATAGGTTGCTTATTGTCACTAATTGTACTTTGCAGACAAGAAAATTATAATATAAGAAACCTTATTCGTTCCCTCTCTATATTGTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGGAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTGAAAGACTGCCAAAAGAGTTATATTTGATTCTTTTATTGTTTGCGTGACACTAACTAGCCAGCTAAAATCAAAAAAAGGGGGTGTATTATacac                                                                                                                                                                               <  2:487363/288‑1 (MQ=255)

                                                                                                                     cgATAATACTTTCAGCATAGAAATATATAATATCATTGATATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTCGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATacac                                                                                                                                                                               <  2:359677/300‑1 (MQ=255)

                                                                                                                     cgATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCGTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATacac                                                                                                                                                                               <  2:862781/300‑1 (MQ=255)

                                                                                                                             cTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTTGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTAtt                                                                                                                                                                       <  1:364835/300‑1 (MQ=255)

                                                                                                                                     aTAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCa                                                                                                                                                               <  1:780347/300‑1 (MQ=255)

                                                                                                                                        aaaaTATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTACCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGNGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCAttt                                                                                                                                                            >  2:205932/1‑300 (MQ=255)

                                                                                                                                          aaTATATAATATCACTTCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATGTCt                                                                                                                                                          <  1:848076/300‑1 (MQ=255)

                                                                                                                                             atataATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAAt                                                                                                                                                       >  1:351839/1‑300 (MQ=255)

                                                                                                                                             atataATATCACTGCTATAGTTTGCTTATTGCCACTAATTTTACGTGGCAGACAAGAAAATGATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATacacctgtctcttatacacatctcgcag                                                                                                                                                       <  1:241414/300‑25 (MQ=255)

                                                                                                                                              tataATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAAtt                                                                                                                                                      <  1:499957/300‑1 (MQ=255)

                                                                                                                                                taataTCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAGTTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCCGTCATTTCTAATTGc                                                                                                                                                    >  2:812782/1‑300 (MQ=255)

                                                                                                                                                        cTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTCCCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTCATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTCCACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATg                                                                                                                                            >  1:141485/1‑300 (MQ=255)

                                                                                                                                                        cTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTCAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATg                                                                                                                                            >  2:340489/1‑300 (MQ=255)

                                                                                                                                                        cTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATg                                                                                                                                            >  2:63776/1‑300 (MQ=255)

                                                                                                                                                        cTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATg                                                                                                                                            >  1:399167/1‑300 (MQ=255)

                                                                                                                                                        cTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGCGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAANAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTNGATATg                                                                                                                                            >  2:744316/1‑300 (MQ=255)

                                                                                                                                                         tGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGc                                                                                                                                           <  2:870052/300‑1 (MQ=255)

                                                                                                                                                          gctctaGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATNAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTCAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAANGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCa                                                                                                                                          >  2:823374/5‑300 (MQ=255)

                                                                                                                                                          gCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCa                                                                                                                                          >  1:225565/1‑300 (MQ=255)

                                                                                                                                                          gCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCACTTTCTCTATTTTTAATCGGGTTGGTTGGTTTAATTTGGTCTTCTGCGGTTAAAGTCGACAACTCGCCCTTTCGTGCTACTTACCATCGTTATTTCACTACTGTCAAAATATTTCTCTTTGGTTCTTTTATTGTTTTCTTGACAATAACTAATCAGCTGAAATTCAAAAAAGAGAGTGTAGTGTACACTTTGCACTCTCCGTCATTTCTAACTGATGGATATgta                                                                                                                                          >  2:198959/1‑298 (MQ=255)

                                                                                                                                                            tataGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATACAAt                                                                                                                                        <  2:845939/300‑1 (MQ=255)

                                                                                                                                                            tagaGTTTGCTTATTGGCACTAATTTTACTCGGCAAACAAGAAAATTATAATATAAAAAACCTTATTCCCCCCCCTTCTATATCTGGGATAGGCTTGCTTGATTTAATTTGGTATTCTGCGGTTAAAGTAGATAATTCGCTAGTGCGTGCTACTTACCATAGGTAGTTAAATACTGCCAAAATATGTATAGTGGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACGTTGTATTCTCTGTCATGTCTAATGGCTGGATATGCAAt                                                                                                                                        <  2:176138/297‑1 (MQ=255)

                                                                                                                                                              taGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGt                                                                                                                                      <  2:521031/300‑1 (MQ=255)

                                                                                                                                                              taGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGt                                                                                                                                      <  1:748921/300‑1 (MQ=255)

                                                                                                                                                               aGTTTGCTTATTGTCACTAATTTTCCGTGGCAGACAAGAAAATTATAATCTAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTCATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTACACTCCAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGTAATGtc                                                                                                                                     >  2:757889/1‑299 (MQ=255)

                                                                                                                                                                  ttGCTTATTGTCACTAATTTTACGTTGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTatat                                                                                                                                  <  2:551879/300‑1 (MQ=255)

                                                                                                                                                                   tGCTTATTGTCACGAATTTTACTTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAANATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTNATTTCTAATTGCTGGATATGCAATGTATAtt                                                                                                                                 <  2:642869/300‑1 (MQ=255)

                                                                                                                                                                      ttATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGNGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAAt                                                                                                                              <  1:650220/300‑1 (MQ=255)

                                                                                                                                                                      ttATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAAt                                                                                                                              <  1:685314/300‑1 (MQ=255)

                                                                                                                                                                      ttATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAAt                                                                                                                              <  2:13843/300‑1 (MQ=255)

                                                                                                                                                                        aTTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAATCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCGTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAg                                                                                                                            <  2:146278/300‑1 (MQ=255)

                                                                                                                                                                           gTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCAt                                                                                                                         >  1:492527/1‑300 (MQ=255)

                                                                                                                                                                                 aaTTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGa                                                                                                                   >  1:492030/1‑300 (MQ=255)

                                                                                                                                                                                                   aGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCttt                                                                                                 <  1:46741/300‑1 (MQ=255)

                                                                                                                                                                                                         ttATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGgtgt                                                                                           >  1:436190/1‑300 (MQ=255)

                                                                                                                                                                                                             aatataaAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGa                                                                                       <  1:785268/300‑1 (MQ=255)

                                                                                                                                                                                                              atataaAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGaa                                                                                      <  2:755310/300‑1 (MQ=255)

                                                                                                                                                                                                              atataaAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGAGAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAANATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGaa                                                                                      <  2:229413/300‑1 (MQ=255)

                                                                                                                                                                                                                       aCCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAg                                                                             >  1:11812/1‑300 (MQ=255)

                                                                                                                                                                                                                        ccTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAgg                                                                            <  1:70456/300‑1 (MQ=255)

                                                                                                                                                                                                                        ccTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAgg                                                                            <  2:578160/300‑1 (MQ=255)

                                                                                                                                                                                                                        ccTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGGTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAgg                                                                            <  1:879178/300‑1 (MQ=255)

                                                                                                                                                                                                                                    ccTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTANCTAGCCAGCTAAAATCAAAAAAAGAGNGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTc                                                                <  2:600577/300‑1 (MQ=255)

                                                                                                                                                                                                                                    catttctacgtttttAATAGGCTTGCTTGAGTTAATTCGGGACTCTGCGTTTAAAGTAGGTAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACGGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGTCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGAGATGCAATGTATATTAATAGCATTCAGGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTc                                                                <  2:235740/290‑1 (MQ=255)

                                                                                                                                                                                                                                       tccTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTcaac                                                             <  2:163768/298‑1 (MQ=255)

                                                                                                                                                                                                                                       tccTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGACAACAGGAGCAGCATATTcaac                                                             <  1:830818/298‑1 (MQ=255)

                                                                                                                                                                                                                                         cTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTcaacaa                                                           <  2:397352/300‑1 (MQ=255)

                                                                                                                                                                                                                                         cTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTcaacaa                                                           <  1:354898/300‑1 (MQ=255)

                                                                                                                                                                                                                                         cTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTNATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTcaacaa                                                           <  1:660764/300‑1 (MQ=255)

                                                                                                                                                                                                                                             atTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAATGCt                                                       >  1:413591/1‑300 (MQ=255)

                                                                                                                                                                                                                                               ttttAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGCAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAATGCTaa                                                     >  1:286798/1‑300 (MQ=255)

                                                                                                                                                                                                                                                          tttcttgATTTAATTTGTTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCGTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAATGCTAATAGGGAtagtt                                          <  1:496996/297‑1 (MQ=255)

                                                                                                                                                                                                                                                          ttgcttgATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAATGCTAATAGGGAtagtt                                          <  1:532182/300‑1 (MQ=255)

                                                                                                                                                                                                                                                               tgATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATCTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAATGCTAATAGGGATAGTTAGTgg                                     <  2:49742/300‑1 (MQ=255)

                                                                                                                                                                                                                                                               tgATTTAATTTGGTATTCTGCGGTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTTTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGGAGGAACGGCAACAGGAGCAGCATATTTAACAATGCTAATAGGGATAGTGAGTgg                                     <  1:894234/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                      aTTTGGTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAATGCTAATAGGGATAGTTAGTGGCGTTGCg                              >  2:270694/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                          ggTATTCTGCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAATGCTAATATGGATAGTTAGTGGCGTTGCGATTc                          <  2:491607/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                 tgtgTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAATGCTAATAGGGATAGTTAGTGGCGTTGCGATTCTTTATAc                   <  1:193218/297‑1 (MQ=255)

                                                                                                                                                                                                                                                                                  gCGTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAATGCTAATAGGGATAGTTAGTGGCGTTGCGATTCTTTATACt                  >  2:446729/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                    gTTTAAAGTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGNATATTCAACAATGCTAATAGGGATAGTTAGTGGCGTTGCGATTCTTTATACTaa                >  2:670012/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                           gTAGATAATTCGCTATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAATGCTAATAGGGATAGTTAGTGGCGTTGCGATTCTTTATACTAAGAAAAat         >  2:576894/1‑300 (MQ=255)

AAACATGCTAACATCCTTTAAACTTCATTCATTGAAACCTTACACTCTGAAATCATCAATGATTTTAGAGATAATAACTTATATATTATGTTTTTTTTCAATGATAATTGCATTCGTCGATAATACTTTCAGCATAAAAATATATAATATCACTGCTATAGTTTGCTTATTGTCACTAATTTTACGTGGCAGACAAGAAAATTATAATATAAAAAACCTTATTCTTCCCCTTTCTATATTTTTAATAGGCTTGCTTGATTTAATTTGGTATTCTGCGTTTAAAGTAGATAATTCGCCATTTCGTGCTACTTACCATAGTTATTTAAATACTGCCAAAATATTTATATTTGGTTCTTTTATTGTTTTCTTGACACTAACTAGCCAGCTAAAATCAAAAAAAGAGAGTGTATTATACACTTTGTATTCTCTGTCATTTCTAATTGCTGGATATGCAATGTATATTAATAGCATTCATGAAAATGACCGCATTTCTTTTGGTGTAGGAACGGCAACAGGAGCAGCATATTCAACAATGCTAATAGGGATAGTTAGTGGCGTTGCGATTCTTTATACTAAGAAAAATCATCCTT  >  NC_000913/3796944‑3797533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`