Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,792,541	C→T	G64E (GGA→GAA)	nlpC ←	NlpC/P60 family lipoprotein NlpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,792,541	0	C	T	100.0%	205.5 / NA	57	G64E (GGA→GAA)	nlpC	NlpC/P60 family lipoprotein NlpC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/28);  total (29/28)

TTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATCCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTG  >  NC_000913/1792257‑1792833                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                     tttCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTATAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCacac                                                                                                                                                                                                                                                                                       <  2:476976/300‑1 (MQ=255) tttCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCacac                                                                                                                                                                                                                                                                                       <  1:64150/300‑1 (MQ=255) tttCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTGTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCacac                                                                                                                                                                                                                                                                                       <  1:132967/300‑1 (MQ=255)  ttCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACAcc                                                                                                                                                                                                                                                                                      <  2:470559/300‑1 (MQ=255)  ttCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACAcc                                                                                                                                                                                                                                                                                      <  1:571988/300‑1 (MQ=255)  ttCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGNTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACAcc                                                                                                                                                                                                                                                                                      <  1:540065/300‑1 (MQ=255)  ttCGACATACTAGATGCGGCGCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTTAGGAACGCATCACTCCCTTTCTGGTATAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGCACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACAcc                                                                                                                                                                                                                                                                                      <  2:287996/300‑1 (MQ=255)      aCATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACAccgcgc                                                                                                                                                                                                                                                                                  <  1:391877/300‑1 (MQ=255)        aTACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAAACATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCCCCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCCTCGTCACAACCACAAATTCCGAACAGTCCCCAccgtgccg                                                                                                                                                                                                                                                                                >  1:154921/1‑300 (MQ=255)         tACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTCAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCCACTCGTCTTTATCCATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCCGCTGCAAACCAAAACGATCGCGCATCGTCCAAACCACAAACTCCGAAAAATCCACAccgcgacgc                                                                                                                                                                                                                                                                               >  2:76845/1‑300 (MQ=255)         tACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCCACTCGTCTTTCTCCATTTGCGTGCCAATCGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAACCGAAACGATTGAGCATCGTCCCAACCAAAAATTCCGAACAGTCCACAccgtgcagc                                                                                                                                                                                                                                                                               >  2:380266/1‑294 (MQ=255)         tACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACAccgcgccgc                                                                                                                                                                                                                                                                               >  1:748452/1‑300 (MQ=255)             aGATTCGTCTAGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTCTCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAACCGAAACGATCGCGCATCGTCACAACCACAAACTCCGAACAGTCCACACCACGCCGCGTCa                                                                                                                                                                                                                                                                           >  1:169818/1‑300 (MQ=255)                     ctcGCCTGCCGGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGNAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTNATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCTAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGccacca                                                                                                                                                                                                                                                                   <  2:587366/300‑1 (MQ=255)                                gAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACgg                                                                                                                                                                                                                                                        <  1:73199/300‑1 (MQ=255)                                      tttCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTATAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGtgcc                                                                                                                                                                                                                                                  <  2:410670/300‑1 (MQ=255)                                           gCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGtgccatgcc                                                                                                                                                                                                                                             <  2:13016/300‑1 (MQ=255)                                                 aGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCCACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGNGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCttt                                                                                                                                                                                                                                       >  1:546344/1‑300 (MQ=255)                                                            aGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCAAAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCg                                                                                                                                                                                                                            >  1:203720/1‑300 (MQ=255)                                                                 ggAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGATTGTTGTTTGGTTTCAAGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAAACACAAATTCCGAACAGTCCCCACCGCGCCGCGTCATGCCACCATAAAGATACGGGGTGCCATGCCAGATTTGTAGCTGGTCGTTCaa                                                                                                                                                                                                                       >  1:709051/1‑300 (MQ=255)                                                                    aCGCATCACTCCCTTGCTGGTAGAGTCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAAcc                                                                                                                                                                                                                    <  2:458869/300‑1 (MQ=255)                                                                      gCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTTGTATCGTAAACACCTACATGCAAACCCTTTTGTCCGGACCCCATTTTGAAAAAGAACAGGTCACCTTGCAGCAACTCGTCTTTATCAAGTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCAAGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAAACACAAATTCCGAACAGTCCCCACCGCGCCGCGTTATGTCACCATAACGATACGGTGTGCCATGTCCGATTTGTAGCTGGTCGtttcaaccag                                                                                                                                                                                                                  >  2:74757/1‑292 (MQ=255)                                                                              cccTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCCATTTGCGTGCCAATAGAGGCTTGTTGTTTTGTTTCCCGGGGCAGCTGCAAATCGAAACGATCGCGCANCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACg                                                                                                                                                                                                          >  1:529595/1‑300 (MQ=255)                                                                              cccTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACg                                                                                                                                                                                                          <  1:751477/300‑1 (MQ=255)                                                                                cTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCCATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCAAAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGt                                                                                                                                                                                                        >  1:462136/1‑300 (MQ=255)                                                                                cTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCCCAAATTCCGAACAGTCCCCACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAAAGGt                                                                                                                                                                                                        >  1:429084/1‑300 (MQ=255)                                                                                      ggTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGTCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGa                                                                                                                                                                                                  >  2:634146/1‑300 (MQ=255)                                                                                       gTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTCCATGCCAACCATTTTTTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCCGCAACTCGTCTTTCTCAATTTGCGTGTCAACAGAGGCTTGTTGTTTGGTTTCAAAGGGAAGCTGCAAATCAAAACGATTGTGCATCGTCACAAACAAAAATTCCGAACAGTCTACACCGCGCCGCGTCATGCCAACCTAGCGATACGGCGTGTCATACAAGATTTGTCGCTGGTCGTTCAAACCAGCAACAACGGTAATCGaa                                                                                                                                                                                                 >  2:116943/1‑300 (MQ=255)                                                                                              cGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATNTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGaaa                                                                                                                                                                                          <  1:540384/300‑1 (MQ=255)                                                                                                       aTTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCAt                                                                                                                                                                                 <  2:436164/300‑1 (MQ=255)                                                                                                           gttgttGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAAAGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGAATTTgg                                                                                                                                                                             >  2:720417/1‑300 (MQ=255)                                                                                                                      cGTAAATACCTCCATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCAAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCCTAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCACACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCtt                                                                                                                                                                  >  1:154054/1‑300 (MQ=255)                                                                                                                         aaaTACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTAt                                                                                                                                                               <  2:418621/300‑1 (MQ=255)                                                                                                                           aTACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACTGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTAtgg                                                                                                                                                             <  1:141019/300‑1 (MQ=255)                                                                                                                           aTACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTAtgg                                                                                                                                                             <  1:60154/300‑1 (MQ=255)                                                                                                                                     gCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACAcc                                                                                                                                                   <  1:75058/300‑1 (MQ=255)                                                                                                                                      cAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACAccc                                                                                                                                                  <  1:657000/300‑1 (MQ=255)                                                                                                                                                tGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGc                                                                                                                                        <  1:626509/300‑1 (MQ=255)                                                                                                                                                tGTCCGGACCCCGTTGTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGc                                                                                                                                        <  1:249054/300‑1 (MQ=255)                                                                                                                                                                 gAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCCAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAAt                                                                                                                       >  1:238527/1‑300 (MQ=255)                                                                                                                                                                                  cTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTc                                                                                                      <  1:725560/300‑1 (MQ=255)                                                                                                                                                                                    ggCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTcag                                                                                                    >  2:268392/1‑298 (MQ=255)                                                                                                                                                                                                       cAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGtttttt                                                                                 >  1:566198/1‑300 (MQ=255)                                                                                                                                                                                                                   cAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTa                                                                     >  1:24021/1‑300 (MQ=255)                                                                                                                                                                                                                    aaTAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTaa                                                                    >  1:469216/1‑300 (MQ=255)                                                                                                                                                                                                                     aTAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAANATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAAt                                                                   >  2:558351/1‑300 (MQ=255)                                                                                                                                                                                                                            cTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGcgt                                                            <  1:551403/300‑1 (MQ=255)                                                                                                                                                                                                                                 tgttTGGTTTCACGTGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACNGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTaa                                                       <  2:663212/300‑1 (MQ=255)                                                                                                                                                                                                                                       gTTTCACGGGGCCGCTGCAAATCGCAACGATCGAGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGCCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGCACGGTTACTCTGTTTTTTATTCTTGCATTACTTTAGCGTCGTAATTAccc                                                 >  2:244159/1‑300 (MQ=255)                                                                                                                                                                                                                                               gggCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTNCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGNTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCa                                         >  2:700666/1‑300 (MQ=255)                                                                                                                                                                                                                                                 gCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAg                                       <  1:634546/300‑1 (MQ=255)                                                                                                                                                                                                                                                               aaaCGATCGCGCATCTTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGaaagc                         <  2:236872/300‑3 (MQ=255)                                                                                                                                                                                                                                                               aaaCGATCGCGCATCGTCACAACCAAAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATc                         >  2:15982/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        cgcATCGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCg                >  1:52204/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             cGTCACAACCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATc           >  1:734719/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    aCCACAAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGGTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGCAATCAGATGGTCGAAATCAGCATTc    >  2:447564/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      cacaAATTCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCANTTGGCGGCGGTGCTTTATGGTGGCTANACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCtg  >  2:494406/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                     TTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGTCCGGACCCCGTTTTGAAAAAGACCAGGTCACCTGGCAGCAACTCGTCTTTATCAATTTGCGTGCCAATAGAGGCTTGTTGTTTGGTTTCACGGGGCAGCTGCAAATCGAAACGATCGCGCATCGTCACAACCACAAATCCCGAACAGTCCACACCGCGCCGCGTCATGCCACCATAACGATACGGCGTGCCATGCCAGCTTTGTAGCTGGTCGTTCAAACCGGCAATAACGGTAATCGAATCAGAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTG  >  NC_000913/1792257‑1792833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑