Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	292,019	C→T	L174L (CTA→TTA)	yagJ →	protein YagJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	292,019	0	C	T	100.0%	88.8 / NA	26	L174L (CTA→TTA)	yagJ	protein YagJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/9);  total (17/9)

AGGTGAAAAGGAAGCTGACCTTCCAGTCTGGAAACACCATTTGTTGTGTGACGAAAGCGGGAACTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCCTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCA  >  NC_000913/291736‑292301                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                           aGGTGAAAAGGAAGCTGACCTTCCAGTCTGGAAACACCATTTGTTGTGTGACGAAAGCGGGAACTATCCGGCTCTGTTGAACCATTGTGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGc                                                                                                                                                                                                                                                                            <  1:757517/300‑1 (MQ=255)   gTGAAAAGGAAGCTGACCTTCCAGTCTGGAAACACCATTTGTTGTGTGACGAAAGCGGGAACTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCtt                                                                                                                                                                                                                                                                          >  1:205872/1‑300 (MQ=255)           gAAGCTGACCTTCCAGTCTGGAAACACCATTTGTTGTGTGACGAAAGCGGGAACTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCaaa                                                                                                                                                                                                                                                                  >  1:235263/1‑300 (MQ=255)                           tCTGGAAACACCATTTGTTGTGTGACGAAAGCGGGAACTATCCGGCTCTGTTGACCCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTgcgc                                                                                                                                                                                                                                                  <  2:706317/300‑1 (MQ=255)                                                             gAACTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCNCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTNCAGGcg                                                                                                                                                                                                                >  2:621123/1‑300 (MQ=255)                                                                                                  gTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGACGAAGTAAAGGGTAAATTACGa                                                                                                                                                                           >  2:343313/1‑300 (MQ=255)                                                                                                   tttttGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAg                                                                                                                                                                          <  1:64426/300‑1 (MQ=255)                                                                                                           aTCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGGAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTGGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGAt                                                                                                                                                                  <  2:73663/300‑1 (MQ=255)                                                                                                                                   gCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGAt                                                                                                                                          <  1:693665/300‑1 (MQ=255)                                                                                                                                     tttCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGc                                                                                                                                        <  1:316805/300‑1 (MQ=255)                                                                                                                                                       acaGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAGTGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGTGCATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTGGATTTGAAACAGCAGGCTGTGCAGGATGCTGTTGCTACCGCTGaaaa                                                                                                                      >  2:206695/1‑300 (MQ=255)                                                                                                                                                          gTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGc                                                                                                                   >  2:219206/1‑300 (MQ=255)                                                                                                                                                          gTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGCCCCGATTTCCTGTTCTTTGCCGAACAGGATGGAAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGTTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAAAGGAAGGCTGTGCAGGATGCTGTTGCTACCGCTGAAAATGc                                                                                                                   >  2:133337/1‑300 (MQ=255)                                                                                                                                                          gTACACAGGACAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGACGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGc                                                                                                                   >  2:341024/1‑300 (MQ=255)                                                                                                                                                            acacAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAg                                                                                                                 >  1:293168/1‑300 (MQ=255)                                                                                                                                                                 gggCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACg                                                                                                            >  2:10756/1‑300 (MQ=255)                                                                                                                                                                                 gAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGAc                                                                                            >  2:572078/1‑300 (MQ=255)                                                                                                                                                                                     cGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCAGATTTCCTGTTCTTTGCCGAACAGGATGGAAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGc                                                                                        >  2:375280/1‑300 (MQ=255)                                                                                                                                                                                                       aCAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATc                                                                      <  2:158212/300‑1 (MQ=255)                                                                                                                                                                                                                                ttCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTg                                             >  1:6430/1‑300 (MQ=255)                                                                                                                                                                                                                                               gAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAAtttt                              >  2:357842/1‑300 (MQ=255)                                                                                                                                                                                                                                                    ggATGGCAAAATGGTTGTGGACCTGGTAGATNCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAANCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGaa                         >  2:520319/1‑300 (MQ=255)                                                                                                                                                                                                                                                           aaaaTGTTTGTGGACCTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATAc                  <  2:735046/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         ccTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTtct    >  1:594949/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          cTGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTtctc   <  1:695130/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           tGGTAGATCCACATAGCTTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCa  >  1:304417/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                           AGGTGAAAAGGAAGCTGACCTTCCAGTCTGGAAACACCATTTGTTGTGTGACGAAAGCGGGAACTATCCGGCTCTGTTGAACCATTGGGAAACCAAGGTTTTTGAGATCGAAACCAAACGTGAAGGATTTGCTTTCTGGTATCGTAATCCACAGTACACAGGGCAGTCGTCACTGGGAATCGCTTATGTTGAAGCTGAACAGTACAAGATTGTTCGTCCCGATTTCCTGTTCTTTGCCGAACAGGATGGCAAAATGGTTGTGGACCTGGTAGATCCACATAGCCTACATCTGGCTGATGCTTTGCCCAAACTGGAAGGACTTGCGCTATATGCCGAACATCATTCTGATGCTTACAGGCGAATCGAATCTGTCGCCGAAGTAAAGGGTAAATTACGAGTGTTAGATTTGAAACGGCAGGATGTGCAGGATGCTGTTGCTACCGCTGAAAATGCAGAAACGTTATTTAGTAGCGGACTTGCTGATGACTATCAGTAATCTATAGAAAATTGCGTAACGAAACTGCATTTTAGTAATTTTTTGAAACAATACAAATAAGGCCTTCTCA  >  NC_000913/291736‑292301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑