Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,998,170	C→T	T63I (ACC→ATC)	uvrD →	DNA helicase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,998,170	0	C	T	100.0%	195.8 / NA	54	T63I (ACC→ATC)	uvrD	DNA helicase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/25);  total (29/25)

GCATCTCTGACCTCGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGC  >  NC_000913/3997876‑3998451                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                          gCATCTCTGACCTCGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATcaaca                                                                                                                                                                                                                                                                                      >  1:441508/1‑300 (MQ=255)                  gATATCATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAACGGCGGCGGAGATg                                                                                                                                                                                                                                                                     >  1:115538/1‑300 (MQ=255)                  gATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATATTCGATTATGGCGGTGACGTTTATCAACAAAGCGGTGGCGGAGATg                                                                                                                                                                                                                                                                     >  2:28221/1‑300 (MQ=255)                     ataATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGt                                                                                                                                                                                                                                                                  <  2:733188/300‑1 (MQ=255)                               aaTCTGTATATATACCCAGCTTTTTGGCGGANGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGANGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCg                                                                                                                                                                                                                                                        >  2:522259/1‑300 (MQ=255)                                      atatataCCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGNATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACt                                                                                                                                                                                                                                                 >  1:513916/1‑300 (MQ=255)                                       tatataCCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTg                                                                                                                                                                                                                                                <  1:25960/300‑1 (MQ=255)                                                         gCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGc                                                                                                                                                                                                                              <  1:633890/300‑1 (MQ=255)                                                              gggCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTAt                                                                                                                                                                                                                         <  1:662918/300‑1 (MQ=255)                                                                gCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTAtgt                                                                                                                                                                                                                       <  1:125236/300‑1 (MQ=255)                                                                gCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTAACTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTAtgt                                                                                                                                                                                                                       >  2:253876/1‑300 (MQ=255)                                                                   ttGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTggg                                                                                                                                                                                                                    >  1:466847/1‑300 (MQ=255)                                                                   ttGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGCATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTggg                                                                                                                                                                                                                    <  2:66055/300‑1 (MQ=255)                                                                          tctcAGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACACACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTATGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAAATGCTCGCAATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCAGGCAAATGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCAc                                                                                                                                                                                                             >  2:260866/1‑300 (MQ=255)                                                                               gCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTACTGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGGGCCAGGGCGGTATATGGGTCGGCACCTTcc                                                                                                                                                                                                        >  2:115976/1‑300 (MQ=255)                                                                                    aCCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCTAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGTAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACggg                                                                                                                                                                                                   <  1:665412/300‑1 (MQ=255)                                                                                    aCCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACggg                                                                                                                                                                                                   <  1:195501/300‑1 (MQ=255)                                                                                                gcggcgGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGt                                                                                                                                                                                       <  1:663982/300‑1 (MQ=255)                                                                                                    cggTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTtgc                                                                                                                                                                                   <  1:656490/300‑1 (MQ=255)                                                                                                      gTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTtgctg                                                                                                                                                                                 <  1:90580/300‑1 (MQ=255)                                                                                                                acatttCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGCGGCCGCGCCACGCATCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCAGCGTATCGTCTGGTTGAGGAGCGTGGAAAANTGCTCGCCATACTCGATTATGGCGGTNACGTCTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTCCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCAcc                                                                                                                                                                       <  2:486900/297‑1 (MQ=255)                                                                                                                 cGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTTGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCa                                                                                                                                                                      >  1:696168/1‑300 (MQ=255)                                                                                                                   tttCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCata                                                                                                                                                                    >  2:401678/1‑300 (MQ=255)                                                                                                                   tttCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCata                                                                                                                                                                    >  1:211061/1‑300 (MQ=255)                                                                                                                   tttCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCAGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCata                                                                                                                                                                    >  1:690818/1‑300 (MQ=255)                                                                                                                   ctgcgagatgtgtataagagacagaTGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCCACCTTCTGGTGCTGGTGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGAGGTGACGTTTATCACCAAAGCAGCGGCGGAGATGCGTCCTCTTCTCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGTACCTTCCACAGGCTGGCGCACCATTTGCTGCGTGCGCAccctc                                                                                                                                                                    >  1:124632/25‑297 (MQ=255)                                                                                                                     tCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATg                                                                                                                                                                  <  1:235345/300‑1 (MQ=255)                                                                                                                      cTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATgg                                                                                                                                                                 <  1:475605/300‑1 (MQ=255)                                                                                                                       ttACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGa                                                                                                                                                                <  1:632475/300‑1 (MQ=255)                                                                                                                               tCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATc                                                                                                                                                        <  1:121062/300‑1 (MQ=255)                                                                                                                                cGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCt                                                                                                                                                       <  2:575589/300‑1 (MQ=255)                                                                                                                                           aTGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAGGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACATTTATCACCAAAGCGGCGGCGGAGATGCGTCGTCGTATTGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGAGCACCATATGGACGCCAATCTGCCGCCGGAtt                                                                                                                                            >  2:145718/1‑300 (MQ=255)                                                                                                                                           aTGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGAGGGCAGTGGTAAGACGAGCGTACTGGTGAATCGTATCGCATGGTTGGTGAGCGTGGAAAACTGCTCGCCATATTTGATTATGGCGGTGACGTTTATCCCCAAAGCGACGGCGGCGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCAAGGGCGGTATGTGGGTTGGCACCTTCCACGGGCTGGCGAACCGTTTGCTGCGTGCGCACCATATGGACGCAAATCTGCAGCAGGAtt                                                                                                                                            >  1:299225/1‑300 (MQ=255)                                                                                                                                                     gcgcGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGTGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCt                                                                                                                                  >  1:248118/1‑300 (MQ=255)                                                                                                                                                         gAAGCGGTTGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGAc                                                                                                                              <  1:380264/300‑1 (MQ=255)                                                                                                                                                            gCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTACGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGt                                                                                                                           <  2:209764/300‑1 (MQ=255)                                                                                                                                                                tGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGCTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAg                                                                                                                       <  2:636994/300‑1 (MQ=255)                                                                                                                                                                             cagccACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGTGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCCGGATTTCCAGATCCTCGCCAGTGAAGACCAGCTACGCCt                                                                                                          >  2:36647/1‑300 (MQ=255)                                                                                                                                                                                  aCCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGTGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCATCATCGTATCAGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCACAGGATTTCCAGATTCTCGACAGTGAAGACCAGTTACGCCTGCTTa                                                                                                     >  2:240139/1‑300 (MQ=255)                                                                                                                                                                                                  gggtgcgggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATNAGCGTGGAGAACTGCTCGCCATACTCGCTTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGc                                                                                     <  2:557917/296‑1 (MQ=255)                                                                                                                                                                                                   ggcgcgggcAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGcc                                                                                    >  1:212908/1‑300 (MQ=255)                                                                                                                                                                                                           cAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCNCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCAATGAACCt                                                                            >  2:554245/1‑300 (MQ=255)                                                                                                                                                                                                             gTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCAATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCAAATCTGCCGCAGGATTCCCAGATCCTCGACCGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTcg                                                                          >  2:108393/1‑300 (MQ=255)                                                                                                                                                                                                               ggTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTcgac                                                                        >  1:225367/1‑300 (MQ=255)                                                                                                                                                                                                                gTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTcgacg                                                                       <  1:466086/300‑1 (MQ=255)                                                                                                                                                                                                                          cgTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTg                                                             <  1:669242/300‑1 (MQ=255)                                                                                                                                                                                                                                ggTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGgccgcc                                                       >  1:107668/1‑300 (MQ=255)                                                                                                                                                                                                                                            cGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAAt                                           >  2:626033/1‑300 (MQ=255)                                                                                                                                                                                                                                                     gacgaGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAATCGGCGGCGGAGATGCTTCATCGTATCGTGCAACTGATGGACACGAGCCAGGGCGNTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACNCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGTGGTAccc                                  <  2:591351/297‑3 (MQ=255)                                                                                                                                                                                                                                                      atgaGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGTGGTACATc                                 <  1:249503/300‑1 (MQ=255)                                                                                                                                                                                                                                                          gCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGTGGTACATCAACa                             >  1:732991/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             aTACTCGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCt          >  1:236419/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  cGATTATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTc     >  1:420187/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     ttATGGCGGTGACGTTTATCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGc  >  2:713793/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                          GCATCTCTGACCTCGCTGATATAATCAGCAAATCTGTATATATACCCAGCTTTTTGGCGGAGGGCGTTGCGCTTCTCCGCCCAACCTATTTTTACGCGGCGGTGCCAATGGACGTTTCTTACCTGCTCGACAGCCTTAATGACAAACAGCGCGAAGCGGTGGCCGCGCCACGCAGCAACCTTCTGGTGCTGGCGGGCGCGGGCAGTGGTAAGACGCGCGTACTGGTGCATCGTATCGCCTGGTTGATGAGCGTGGAAAACTGCTCGCCATACTCGATTATGGCGGTGACGTTTACCAACAAAGCGGCGGCGGAGATGCGTCATCGTATCGGGCAACTGATGGGCACGAGCCAGGGCGGTATGTGGGTCGGCACCTTCCACGGGCTGGCGCACCGTTTGCTGCGTGCGCACCATATGGACGCCAATCTGCCGCAGGATTTCCAGATCCTCGACAGTGAAGACCAGCTACGCCTGCTTAAGCGTCTGATCAAAGCCATGAACCTCGACGAGAAGCAGTGGCCGCCGCGGCAGGCAATGTGGTACATCAACAGCCAGAAAGATGAAGGCCTGCGTCCGC  >  NC_000913/3997876‑3998451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑