Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,602,271	C→T	L159L (CTG→CTA)	ftsE ←	cell division protein FtsE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,602,271	0	C	T	100.0%	208.0 / NA	61	L159L (CTG→CTA)	ftsE	cell division protein FtsE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/41);  total (20/41)

CGTTTTGGTGCGTTACGACCGCCGTCGCCTGAGCCGCCGACCGATTTACGGAAGCGATCAAGACGCCCGCCAAACTGCCGAATATGATTGATTGCATCGCGCTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGCAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGC  >  NC_000913/3601977‑3602560                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                  cgTTTTGGTGCGTTACGACCGCCGTCGCCTGAGCCGCCGACCGATTTACGGAAGCGATCAATACGCCCGCCAAACTGCCGAATATGATTGATTGCATCGCGCTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTAc                                                                                                                                                                                                                                                                                              <  2:712556/300‑1 (MQ=255) cgTTTTGGTGCGTTACGACCGCCGTCGCCTGAGCCGCCGACCGATTTACGGAAGCGATCAAGACGCCCGCCAAACTGCCGAATATGATTGATTGCATCGCGCTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTAc                                                                                                                                                                                                                                                                                              <  1:111860/300‑1 (MQ=255)                   ccgccgTCGCCTGAGCCGCCGACCGATTTACGGAAGCGATCAAGACGCCCGCCAAACTGCCGAATATGATTGATTGCATCGCGCTTATTCATGGCCCACGCCTCCATGCAAGTCACCATCGCTCAGGGTGAGCATGCGATAGGAACGCNGCGAGATCAGGTTGATGTCGTGCGTTNCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTcaccac                                                                                                                                                                                                                                                                            <  2:487898/300‑1 (MQ=255)                     gccgTCGCCTGAGCCGCCGACCGATTTACGGAAGCGATCAAGACGCCCGCCAAACTGCCGAATATGATTGATTGCATCGCGCTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACcg                                                                                                                                                                                                                                                                          >  1:120472/1‑300 (MQ=255)                                  ccgccgACCGATTTACGGAAGCGATCAAGACGCCCGCCAAACTGCCGAATATGATTGATTGCATCGCGCTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGcc                                                                                                                                                                                                                                                             <  1:582551/300‑1 (MQ=255)                                       gaccgaTTTACGGAAGCGATCAAGACGCCCGCCAGACTGCCGAATATGATTGATTGCATCGCGCTTATTCATGGCCCACGCCGCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAacac                                                                                                                                                                                                                                                        <  2:395164/300‑1 (MQ=255)                                                 cGGAAGCGATCAAGACGCCCGCCAAACTGCCGAATATGATTGATTGCATCGCGCTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTc                                                                                                                                                                                                                                              <  2:200793/300‑1 (MQ=255)                                                 cGGAAGCGATCAAGACGCCCGCCAAACTGCCGAATATGATTGATTGCATCGCGCTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTc                                                                                                                                                                                                                                              <  1:32725/300‑1 (MQ=255)                                                     aGCGATCAAGACGCCCGCCAAACTGCCGAATATGATTGATTGCATCGCGCTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTACAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCg                                                                                                                                                                                                                                          >  2:339685/1‑300 (MQ=255)                                                      gCGATCAAGACGCCCGCCAAACTGCCGAATATGATTGATTGCATCGCGCTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGc                                                                                                                                                                                                                                         >  1:420787/1‑300 (MQ=255)                                                        gATCAAGACGCCCGCCAAACTGCCGAATATGATTGATTGCATCGCGCTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGccc                                                                                                                                                                                                                                       >  1:355356/1‑300 (MQ=255)                                                        gATCAAGACGCCCGCCAAACTGCCGAATATGATTGATTGCATCGCGCTTATCCATGGCCCACGCCTCCATGCAAGTGAACATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATAAGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGcac                                                                                                                                                                                                                                       >  2:668727/1‑298 (MQ=255)                                                                    cgccAAACTGCCGAATATGATTGATTGCATCGCGCTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATa                                                                                                                                                                                                                           >  1:685582/1‑300 (MQ=255)                                                                                  atatGATTGATTGCATCGCGCTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGttcttctc                                                                                                                                                                                                             <  1:448624/300‑3 (MQ=255)                                                                                  atatGATTGATTGCATCGCGCTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGc                                                                                                                                                                                                             >  1:91028/1‑300 (MQ=255)                                                                                            ttgCATCGCGCTTATTCATGGCCCACGCCTACATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCCGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGt                                                                                                                                                                                                   >  1:400796/1‑300 (MQ=255)                                                                                              gCATCGCGCTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAg                                                                                                                                                                                                 <  1:420092/300‑1 (MQ=255)                                                                                                    cgcTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGAc                                                                                                                                                                                           >  1:251429/1‑300 (MQ=255)                                                                                                     gcTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACt                                                                                                                                                                                          <  2:631679/300‑1 (MQ=255)                                                                                                      cTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCACGaatt                                                                                                                                                                                         >  1:57505/1‑297 (MQ=255)                                                                                                           tCATGGCCCACGCCTCCATGTAAGTGACCATCGCTCAGTGTGAGCATTCGATGTGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTAAAACATACGTAAAATGCCTTCCGACAGCGCGTCGTCCATGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGGTGTTCACCGCCCGAAAGCTGAATATGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATcc                                                                                                                                                                                    <  2:7785/300‑1 (MQ=255)                                                                                                           tCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGTAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATcc                                                                                                                                                                                    <  1:279208/300‑1 (MQ=255)                                                                                                           tCATGACCCACGCCTCCATTCAATTTACCATCGCTCAGGGTGAGCATGCAATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCTTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATATGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTGTcc                                                                                                                                                                                    <  2:95601/300‑1 (MQ=255)                                                                                                                gCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGACCGCCGCGAGATCAGGTTGATGTCGTGCGTTGCAATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGNCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAgcgc                                                                                                                                                                               >  2:655733/1‑300 (MQ=255)                                                                                                                          ccATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACAcc                                                                                                                                                                     <  2:359308/300‑1 (MQ=255)                                                                                                                                    gACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCCGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGAGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGCATAGGGAAGTTCTTCGCTCTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGaa                                                                                                                                                           >  2:245832/1‑300 (MQ=255)                                                                                                                                                gggTGAGCATGTGATAGGAACGCAGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGACAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGTTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTAGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCAGCCGACACCCTGCGGCGAATATCGTCACCGc                                                                                                                                               >  2:264655/1‑300 (MQ=255)                                                                                                                                                gggTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGc                                                                                                                                               >  1:711027/1‑300 (MQ=255)                                                                                                                                                                 gAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATc                                                                                                                              <  1:106209/300‑1 (MQ=255)                                                                                                                                                                                aGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACg                                                                                                               <  1:660072/300‑1 (MQ=255)                                                                                                                                                                                           gTGCGTTGCCATCAATACGGTTACCCCAACGCGTTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCATTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGAc                                                                                                    <  2:151446/300‑1 (MQ=255)                                                                                                                                                                                           gTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGAc                                                                                                    <  1:440023/300‑1 (MQ=255)                                                                                                                                                                                           gTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGAc                                                                                                    <  2:36771/300‑1 (MQ=255)                                                                                                                                                                                           gTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGNAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACANATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGAc                                                                                                    <  2:693466/300‑1 (MQ=255)                                                                                                                                                                                           gTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGGAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGAc                                                                                                    <  1:343400/300‑1 (MQ=255)                                                                                                                                                                                           gTGCGTTGCCATCAATACGGCTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGAc                                                                                                    <  2:332306/300‑1 (MQ=255)                                                                                                                                                                                           gTGCGTNGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGNAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGTCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGAc                                                                                                    <  2:611205/300‑1 (MQ=255)                                                                                                                                                                                                ttGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTAc                                                                                               <  2:201227/300‑1 (MQ=255)                                                                                                                                                                                                ttGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGGCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGTAAGTTCTTCGCTTTGTCCATTAGCCCGACTTTATCCAGCGCCGCCGACACCCGTCGACGAATATCGTCACCGCTGGCACCTGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTAc                                                                                               <  2:236561/300‑1 (MQ=255)                                                                                                                                                                                                   ccATCANTACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCNTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGt                                                                                            <  2:609734/300‑1 (MQ=255)                                                                                                                                                                                                   ccATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCGTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGt                                                                                            <  2:711039/300‑1 (MQ=255)                                                                                                                                                                                                   ccATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGt                                                                                            <  1:108630/300‑1 (MQ=255)                                                                                                                                                                                                          tACGGTTACCCCAACGCGGTTAAACTCTTCGAACAGACGTAAAATGCCTTCCGACAGCGCTTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGTCGACGAATATCTTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAg                                                                                     <  1:247630/300‑1 (MQ=255)                                                                                                                                                                                                          tACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCATTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAg                                                                                     <  2:357964/300‑1 (MQ=255)                                                                                                                                                                                                             ggTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGNCCGAAAGCTGAATAGGGAAGTTCTTCNCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAg                                                                                  <  2:501065/300‑1 (MQ=255)                                                                                                                                                                                                             ggTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATATGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAg                                                                                  <  1:140552/300‑1 (MQ=255)                                                                                                                                                                                                                  cctcAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGt                                                                             <  2:473245/297‑1 (MQ=255)                                                                                                                                                                                                                  ccccAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGt                                                                             <  1:391789/300‑1 (MQ=255)                                                                                                                                                                                                                    ccAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGa                                                                           <  1:40683/300‑1 (MQ=255)                                                                                                                                                                                                                       aCGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCTCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATcc                                                                        <  2:259042/300‑1 (MQ=255)                                                                                                                                                                                                                          cgGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCANCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTgg                                                                     >  1:494400/1‑300 (MQ=255)                                                                                                                                                                                                                                          aCAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTGACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCNTTGTCCAGTAGCCCGACTTTATCCAGNGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCt                                                     <  2:505861/300‑1 (MQ=255)                                                                                                                                                                                                                                             gACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTggc                                                  >  1:562065/1‑300 (MQ=255)                                                                                                                                                                                                                                                             cGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAAc                                  <  2:121462/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    gcgTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCAACACCGCGCGGGCAATGCCAACACGCTGCTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGATTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCCCCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGAAAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAAGCTGGCGGCGCAGAAACGGAACTTCACgg                           >  2:377235/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    gcatcgtcCAGGTTCCCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGTTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTTTTCGATGTGCCCAGTAGCCCGACTTTATCCAGCGCTGCCGACACCCGGCGACGAATATCGTCACCGCCGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGCTCCTGGAAAATCATGCAAATCTGGCGGCGCAGAAAAGGAATTTAACgg                           >  2:396973/4‑300 (MQ=255)                                                                                                                                                                                                                                                                       tcgtcCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGttt                        >  1:430057/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         gtcCAGGTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGttttt                      >  1:344232/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               gTTACCAGTCGGTTCGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGAcg                <  1:319231/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        cGGTTCGTCCGCCATTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACATGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTGATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCAATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTc       <  2:364930/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             cGTCCGCCAGTAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGc  <  1:387986/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                  CGTTTTGGTGCGTTACGACCGCCGTCGCCTGAGCCGCCGACCGATTTACGGAAGCGATCAAGACGCCCGCCAAACTGCCGAATATGATTGATTGCATCGCGCTTATTCATGGCCCACGCCTCCATGCAAGTGACCATCGCTCAGGGTGAGCATGCGATAGGAACGCCGCGAGATCAGGTTGATGTCGTGCGTTGCCATCAATACGGTTACCCCAACGCGGTTAAACTCTTCAAACAGACGTAAAATGCCTTCCGACAGCGCGTCGTCCAGGTTACCAGTCGGTTCGTCCGCCAGCAGTACCGCGGGCTTGTTCACCACCGCGCGGGCAATGCCAACACGCTGTTGTTCACCGCCCGAAAGCTGAATAGGGAAGTTCTTCGCTTTGTCCAGTAGCCCGACTTTATCCAGCGCCGCCGACACCCGGCGACGAATATCGTCACCGCTGGCACCGGCGATAATCAGCGGGATCGCCACGTTATCGTAGACAGTACGGTCCATCAGTAGATGGTGATCCTGGAAAATCATGCCAATCTGGCGGCGCAGAAACGGAACTTCACGGTTTTTCAGACGCGTGATGTCATGGC  >  NC_000913/3601977‑3602560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑